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PENGARUH PENGENDALIAN pH TERHADAP PEMBENTUKAN ETANOL DAN PERGESERAN PRODUK ASIDOGENESA DARI FERMENTASI LIMBAH CAIR INDUSTRI MINYAK SAWIT (The Influence of pH Control on Ethanol and Switch of Acidogenic Products Formation from Palm Oil Mill Effluent)

https://doi.org/10.22146/jml.18719

David Andrio(1*), Mindriany Syafila(2), Marisa Handajani(3), Dessy Natalia(4)

(1) Program Doktor Teknik Lingkungan, Fakultas Teknik Sipil dan Lingkungan, Institut Teknologi Bandung. Jln. Ganesha No. 10, Bandung, 40132.
(2) Kelompok Keahlian Rekayasa Air dan Limbah Cair, Fakultas Teknik Sipil dan Lingkungan, Institut Teknologi Bandung. Jln. Ganesha No. 10, Bandung, 40132.
(3) Kelompok Keahlian Rekayasa Air dan Limbah Cair, Fakultas Teknik Sipil dan Lingkungan, Institut Teknologi Bandung. Jln. Ganesha No. 10, Bandung, 40132.
(4) Kelompok Keahlian Biokimia, Fakultas Matematika dan Ilmu Pengetahuan, Institut Teknologi Bandung. Jln. Ganesha No. 10, Bandung, 40132.
(*) Corresponding Author

Abstract


ABSTRAK

Limbah cair industri minyak sawit memiliki potensi sebagai substrat pembentukan etanol. Pemanfaatan kultur campuran dalam pembentukan etanol memiliki keuntungan karena tidak memerlukan sterilisasi substrat, namun akan dihasilkan berbagai produk samping dan sebaliknya pada Saccharomyces cerevisiae. Penelitian ini bertujuan untuk mempelajari pengaruh pengaturan pH terhadap pembentukan etanol dan produk asidogenesa. Rancangan penelitian terdiri dari reaktor bakteri anaerob dan ragi dengan perlakuan pengendalian pH pada rentang 6-6,5 dan tanpa pengendalian pH dengan pH awal fermentasi 6-6,5. Hasil penelitian menunjukkan Degree Acidification (DA), Total Asam Volatil (TAV) dan etanol tertinggi berurutan sebesar 0,32; 808,03 mg/L dan 24,03 mg/L pada reaktor bakteri dengan pengendalian pH; 0,23; 522,43 mg/L dan 23,12 mg/L pada reaktor tanpa pengendalian pH; 0,25; 775,78 mg/L dan 34,11 mg/L pada reaktor ragi dengan pengendalian pH dan 0,32; 866,71 mg/L dan 29,17 mg/L pada reaktor ragi tanpa pengendalian pH. Pengendalian pH fermentasi meningkatkan pembentukan produk asetil-KoA dari 4,35% menjadi 7,34% pada reaktor bakteri dan dari 17,92% menjadi 18,78% pada reaktor ragi dan tidak berpengaruh terhadap pembentukan etanol. 

ABSTRACT

Palm oil mill effluent has potention for substrate to ethanol formation. Utilization of anaerobic mixed culture bacteria to form ethanol has advantages i.e not requiring sterilization of the substrate and vice versa in Saccharomyces cerevisiae, but resulting side products. The aims of this research are to study effect of controlling pH on ethanol formation and acidogenic products. Design experiment consisted of anaerobic bacteria and yeast reactor with the pH control in the range of pH 6 - 6.5 and initial pH 6-6.5 for without pH control treatment. The results showed the highest Degree Acidification (DA), Total Volatile Fatty Acid (TVFA) and ethanol are 0.32; 808.03 mg/L and 24.03 mg/L for bacteria reactor with pH control; 0.23; 522.43 mg/L and 23.12 mg/L for bacteria reactor without pH control; 0.25; 775.78 mg/L and 34.11 mg/L for yeast reactor with pH control and 0.32; 866.71 mg/L and 29.17 mg/L for yeast reactor without pH control. Controlling pH increasing acetyl-CoA product formation from 4.35% to 7.34% for bacteria reactor and from 17.92% to 18.78% for yeast one and not affect to rising ethanol formation.


Keywords


asam lemak volatil; bakteri kultur campuran anaerob; degree acidification; etanol; keasaman; Saccharomyces cerevisiae; acidity; degree acidification; ethanol volatile fatty acids; mixed culture anaerobic bacteria



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DOI: https://doi.org/10.22146/jml.18719

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