Biodegradation of Polychlorinated Biphenyls Using Acclimadzed Biofilmina Three-Phase Fluidized Bed Aerobic Reactor

Joseph Auresenia(1*), Donna Marie G. Taleon(2)

(1) Asian Regional Research Programme on Environmental College of Engineering, De La Salle University-Manila 2401 Taft Aven ue, 1004 Manila, PHILIPPINES
(2) Asian Regional Research Programme on Environmental College of Engineering, De La Salle University-Manila 2401 Taft Aven ue, 1004 Manila, PHILIPPINES
(*) Corresponding Author


This paper investigates the ability of microorganisms in a biofilm to degrade polychlorinated biphenyls (PCBs) using a three-phase fluidized bed aerobic biofilm reactor. Water was spiked with PCBs that contain mainly Aroclor 1260 to simulate PCB-contaminated water. The "contaminated" water was batch fed into the reactor to acclimatize the microorganisms in the biofilm. The degradation of PCBs was monitored through the decrease in concentration of Aroclor 1260. Samples were analyzed using gas chromatography equipped with an electron capture detector (ECD). Batch experimental runs with an initial concentration of around 70 ppm showed PCB degradation of up to 93% after 8 h. After the runs that usen "contaminated" water, the batch runs using feed that contain easily degradable organic chemicals were performed to determine the effect of contact with PCB on the biofilm. Their latter runs showed that the COD degradation rate had no significant difference with the COD runs before the PCB batch runs. These results showed that biofilm formed in a three-phase fluidized bed Aerobic is capable of degrading PCB in water and that the microorganisms are not significantly affected by exposure to PCB.


Acclimatized biofilm, biodegration, (bio)chemical oxygen demand (BOD/COD) reduction, polychlorinated biphenyls (PCBs), and three-phase fluidized aerobic bed bioreactor.


  1. APHA/AWWA/WEF. (1992), Standard methods for the examination of water and wastewater, 18th ed., American Public Health Association/American Water Works Association/Water Environment Federation, Washington D.C. 
  2. Auresenia, J. (2002). Mathematical modeling and analysis of the reaction kinetics and dynamics of the three-phase fluidized bed biofilm reactor for wastewater treatment. Unpublished Ph.D. Dissertation, Waseda University, Tokyo, Japan.
  3. Auresenia, J., Ang, S., Espinosa, D., and Juan, J. (2002). Formation of biofilm on different particle media using modified kitchen waste extract as substrate. Paper presented at RSCE, Malaysia. 
  4. Edgehill, R. U. (1996). "Degradation of pentachloropheno! (PCP) by Arthrobacter strain ATCC 33790 in biofilm culture.” Water Research, 30, 2, 357-63. 
  5. Erickson, M. P. (1997). Analytical chemistry of PCBs, 2nd ed., CRC-Lewis Publishers, New York 
  6. Estrellan, C. R. E. (2003). Asian Regional Research Programme on Environmental Technology (ARRPET): Gas chroma- tography-electron capture detector (GC ECD) analysis of polychlorinated biphenyls (PCBs) in soil, oil, and water matrices. Practicum Project, Graduate School of the College of Engineering, De La Salle University-Manila, Philippines. 
  7. Fathepure, B. Z., and Vogel, T. M. (1991). "Complete degradation of poly chlorinated hydrocarbons by a two-stage biofilm reactor," Applied and Environmental Microbiology, 57, 12, 3418-22 
  8. Gupta, A. B., and Gupta, S. K. (1999). "Simultaneous carbon and nitrogen removal in a mixed culture aerobic RBC biofilm," Water Research, 33, 2,555-61. 
  9. Hirata, A., Lee, H. S., Tsuneda, S., and Takai, T. (1997). "Treatment of photographic wastewater using anaerobic-aerobic biofilm reactor," Water Sci. Technol., 36, 91-99. 
  10. Mukerjee-Dhar, G., Shimura, M., and Kimbara, K. (1998). "Degradation of poly chlorinated biphenyls by cells Rhodococcus opacus strain TSP203 immobilized in aliginate and in solution," Enzyme and Microbial Technology 23, 34 41. 
  11. Okabe, S., Oozawa, Y., Hirata, K., and Watanabe, Y. (1996). "Relationship between population dynamics of nitrifiers in biofilms and reactor performance at various C:N ratios," Water Research, 30, 7, 1563–72. 
  12. Sanderson, S., and Stewart, P. S. (1997). "Evidence of bacterial adaptation to monochloramine in Pseudomonas aeruginosa biofilms and evaluation of biocide action model," Biotechnol. Bioengrg., 56, 2, 207-9. 
  13. Shen, C. F., and Guiot, S. R. (1996). "Long-term inpact of dissolved O, on the activity of anaerobic granules," Biotechnol. Bioeng., 49, 611-20. 
  14. Taleon, D. M. G., Borja, J. Q., and Auresenia, J. L. (2003), Preliminary study on the biodegradation of PCBs in water by acclimatized biofilm. Paper presented in the ARRPET National Workshop 2003. 
  15. Tartakovsky, B., Michotte, A., Cadieux, J-C. A., Lau, P. C.K., Hawari, J., and Guiot, S. R. (2001). Degradation of Aroclor 1242 in a single-stage coupled anaerobic/ aerobic bioreactor. PII: 90043 1354(01)00175-0. (Paper received thru e-mail by S.R. Guiot upon request). 
  16. Villaverde, S., FDZ-Polanco, F., and Garcia, P. A. (2000). "Nitrifying biofilm acclimation to free ammonia in submerged biofilters, startup influence," Water Research, 34, 2, 602-10. 
  17. Zhang, M., Tay, J. H., Qian, Y., and Gu, X. S. (1998). "Coke plant wastewater treatment by fixed biofilm system for COD and NH -N removal," Water Research, 32, 2, 519-27.


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