Development of Crack-Free Alumina Sol-gel/Poly(vinyl Alcohol) Membranes for Glucose Oxidase Immobilization

https://doi.org/10.22146/ajche.50128

Fui Ling Wong(1*), Azila Abdul Aziz(2)

(1) Department of Bioprocess Engineering, Faculty of Chemical and Natural Resources Engineering, Universiti Teknologi Malaysia (UTM) 81300 UTM Skudai, Johor, MALAYSIA
(2) Department of Bioprocess Engineering, Faculty of Chemical and Natural Resources Engineering, Universiti Teknologi Malaysia (UTM) 81300 UTM Skudai, Johor, MALAYSIA
(*) Corresponding Author

Abstract


A simple procedure to incorporate PVA into alumina sol-gel membrane was investigated as a suitable support material for glucose oxidase. The alumina sol was prepared using aluminum iso-propoxide via the sol-gel process. PVA was employed as the organic binder to enhance the mechanical strength of the fragile sol-gel membranes. The ability of the hybrid membrane to retain glucose oxidase and the apparent enzyme activities were studied. The resulting composite membranes were found to be crack-free, stable, and still very active after 60 days. However, the enzyme leakage period was observed to be quite long. The enzyme was still leaking from the membrane after more than 10 days albeit at a very low level.

Keywords


Glucose Oxidase (GOD), PVA, alumina sol-gel, composite membranes, immobilization, enzyme leakage



References

  1. Abdul-Aziz, A. (2001). Amperometric Glucose Biosensors: Systematic Material Selection and Quantitative Analysis of Performance. The Johns Hopkins University, Baltimore, Maryland: Ph.D. Dissertation.
  2. Ananthakumar, S., Manohar, P., and Warrier, K.G.K. (2004). “Effect of boehmite and organic binders on extrusion of alumina,” Ceram Int, 30, 837-842. 
  3. Andrew, M., David, A., and Pankaj, V. (1996). “Diffusion restricting outer membranes for greatly extended linearity measurements with glucose oxidase enzyme electrodes,” Anal Chim Acta, 333, 223-231.
  4. Brahim, S., Narinesingh, D., and Guiseppi- Elie, A. (2002). “Polypyrrole-hydrogel composites for the construction of clinical important biosensors,” Biosens Bioelectron, 17, 53-59.
  5. Brinker, C.J. and Scherer, G.W. (1990). Sol-Gel Science: The Physics and Chemistry of Sol- Gel Processing. Academic Press, Inc, USA.
  6. Chen, X., Hu, Y., and Wilson, G.S. (2002). “Glucose microbiosensor based on alumina sol-gel matrix/electropolymerized composite membrane,” Biosens Bioelectron, 17, 1005-1013.
  7. Dave, B.C., Dunn, B., Valentine, J.S., and Zink, J. I. (1994). “Sol-gel encapsulation methods for biosensors,” Anal Chem, 66, 1120A-1127A.
  8. Doretti, L., Ferrara, D., Gattolin, P., and Lora, S. (1997). “Amperometric biosensor with physically immobilized glucose oxidase on a PVA cryogel membrane,” Talanta, 44, 859- 866.
  9. Forzani, E.S., and Solis, V.M. (2000). “Electrochemical behavior of polyphenol oxidase immobilized in self-assembled structures layer by layer with cationic polyallylamine,” Anal Chem, 72, 5300- 5307.
  10. Glezer, V., and Lev, O. (1993). “Sol-gel vanadium pentaoxide glucose biosensor,” J. Am Chem Soc, 115, 2533-2534.
  11. Lambert, C.K., and Gonzalez, R.D. (1999). “Effect of binder addition on the properties of unsupported γ–Al2 O3 membranes,” Mater Lett, 38, 145-149.
  12. Lev, O., Tsionsky, M., Rabinovich, L., Glezer, V., Sampath, S., Pankaratov, I., and Gun, L. (1995). “Organically modified sol-gel sensors,” Anal Chem, 67, 22A-30A. Li, J., Tan, S.N., and Ge, H. (1996). “Silica sol- gel immobilized amperometric biosensor for hydrogen peroxide,” Anal Chim Acta, 335, 137-145.
  13. Lillis, B., Grogan, C., Berney, H., and Lane, W.A. (2000). “Investigation into immobilization of lactate oxidase to improve stability,” Sensor Actuat B, 68, 109-114.
  14. Liu, Z., Liu, B., Zhang, M., Kong, J., and Deng, J. (1999). Al2 O3 sol-gel derived amperometric biosensor for glucose,” Anal Chim Acta, 392, 135-141.
  15. Miao, Y., and Tan, S.N. (2001). “Amperometric hydrogen peroxide biosensor with silica sol- gel/chitosan film as immobilization matrix,” Anal Chim Acta, 437, 87-93.
  16. Onda, M., Ariga, K., and Kunitake, T. (1999). “Activity and stability of glucose oxidase in molecular films assembled alternately with polyions,” J Biosci Bioeng, 87, 69-75.
  17. Pfeifer, D. (1997). “Commercial Biosensors for Medical Application.” In: Scheller, F. W.,
  18. Schubert, F., Fedrowitz, J. eds. Frontiers in Biosensorics—II. Practical Applications. Birkhaüser Verlag.
  19. Venkatesh, R., and Ramanan, S.R. (2000). “Effect of organic addition on the properties of sol-gel spun alumina fibers,” J. Eur Ceram Soc, 20, 2543-2549.
  20. Wang, B., Li, B., Deng, Q., and Dong, S. (1998). ”Amperometric glucose biosensor based on sol-gel organic–inorganic hybrid material,” Anal Chem, 70, 3170-3174.
  21. Wong, F.L., and Azila, A.A. (2004, December 8-9). “Immobilization of glucose oxidase on poly(vinyl alcohol): The effect of immobilization temperature on apparent enzyme activity.” 1st National Postgraduate Colloquium (NAPCOL), The Gurney Resort Hotel & Residence, Penang, Malaysia.
  22. Yağar, H., and Sağiroğlu, A. (2002). Noncovalent immobilization of quince (Cydonia oblonga) polyphenol oxidase on alumina,” Acta Chim. Slov, 49, 893-902.
  23. F.-L. Wong and Abdul-Aziz 67 Yang, W.P., Shyu, S.S., Lee, E. S., and Chao, A.C. (1996). “Effects of PVA content and calcination temperature on the properties of PVA/boehmite composite film,” Materials Chemistry and Physics, 45, 108-113.
  24. Yang, X. H., Hua, L., Gong, H. Q., and Tan, S.N. (2003). “Covalent immobilization of an enzyme (glucose oxidase) onto a carbon sol-gel silicate composite surface as a biosensing platform,” Anal Chim Acta, 478, 67-75.
  25. Yoldas, B.E. (1975). “Alumina sol preparation from alkoxides,” Ceramic Bulletin, 54, 289- 290.



DOI: https://doi.org/10.22146/ajche.50128

Article Metrics

Abstract views : 24

Refbacks

  • There are currently no refbacks.