γ-Alumina Doped Alginate Gel for Cell Immobilization in Fermentation Processes

https://doi.org/10.22146/ajche.50089

Jirawan Mongkolkajit(1), Jiranan Pullsirisombat(2), Seeroong Prichanont(3), Veerapat Tantayakom(4), Phatthanon Prasitchoke(5), Muenduen Phisalaphong(6*)

(1) Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, 10330, Thailand
(2) Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, 10330, Thailand
(3) Department of Chemical Engineering, Faculty of Engineering, Chulalongkorn University, Bangkok, 10330, Thailand
(4) PTT Chemical Public Company Ltd, Thailand
(5) PTT Chemical Public Company Ltd, Thailand
(6) PTT Chemical Public Company Ltd, Thailand
(*) Corresponding Author

Abstract


γ-Alumina (γ-Al2O3) doped alginate gel (AEC) was developed as a cell carrier in fermentation processes of Saccharomyces cerevisiae M.30 for ethanol production and Clostridium butyricum DSM 5431 for 1,3-propanediol production. In a single batch system of ethanol fermentation, the final ethanol concentration of suspended cell (SC), immobilized cell on γ-Al2O3 (AC) and AEC cultures were 82.4, 77.1 and 74.6 g/l, respectively. In 4-cycle repeated batch fermentation, the AEC culture demonstrated a good potential of reusability. Its ethanol production and conversion yield of the 1st, 2nd and 3rd repeated batch were comparable to those of the SC and AC cultures with the immobilization yield of 86%. AEC was also found to be effective for the cell immobilization of C. butyricum with the immobilization yield of 83%. However, the strong inhibition effect of cell-γ-Al2O3 immobilization towards 1,3-propanediol production was observed. Moreover, 1, 3-propanediol fermentation stability in the SC, AC and AEC systems tended to be lowered during the repeated batch fermentation. Interfering of positive charge of γ-Al2O3 on the cell membrane was thought to be the cause of the inactivity of C. butyricum DSM 5431 in 1,3-propanediol production.

Keywords


γ-Alumina, alginate, Clostridium butyricum, immobilization, Saccharomyces cerevisiae



References

  1. Arasaratnam, V. (1994). “Nutrients along with calcium in glucose feed enhance the life of alginate entrapped yeast cells,” Process Biochem., 29, 253-256.
  2. Bai, F.W., Anderson, W.A., and Moo-Young, M. (2008). “Ethanol fermentation technologies from sugar and starch feedstocks,” Biotech. Adv., 26, 89-105.
  3. Eggersdorfer, M., Meyer, J., Eckes, P. (1992). “Use of renewable resources for non-food materials,” FEMS Microbiol Rev., 103, 355- 364.
  4. Kana, K., Kanellaki, M., Papadimitriou, A., Psarianos, C., Koutinas, A.A. (1989) “Immobilization of Saccharomyces cerevisiae on γ-Alumina Pellets and Its Ethanol Production in Glucose and Raisin Extract Fermentation,” J. Ferment. Bioeng., 68, 213- 215.
  5. Kanellaki, M., Koutinas, A.A., Kana, K., Nicolopoulou, M., Papadimitriou, A., Lycourghiotis, A. (1989). “Ethanol production by Saccharomyces cerevisiae promoted by γ-Alumina,” Biotechnology and Bioengineering, 34, 121-125.
  6. Kourkoutasa, Y., Bekatoroua, A., Banatb, I.M., Marchantb, R., and Koutinasa, A.A. (2004). “Immobilization technologies and support materials suitable in alcohol beverages production: a review,” Food Microbio., 21, 377-397
  7. Koutinas, A.A., Kanellaki, M., Lycourghiotis, A., Typas, M.A., Drainas, C. (1988). “Ethanol production by Zymomonas mobilis entraped in alumina pellets,” Appl. Microbiol. Biotechnol., 28, 235-239.
  8. Meesters, P. A. E. P., Huijberts, G. N. M., Eggink, G. (1996). “High cell density cultivation of the lipid accumulating yeast Cryptococcus curvatus using glycerol as a carbon source,” Appl. Microbiol. Biotechnol., 45, 575-579.
  9. Miller, G. L. (1959). “Use of dinitrosalicylic acid reagent for determination reducing sugar,” Anal. Chem., 31, 426–428.
  10. Nguyen, T.L.T., Gheewala, S.H., Garivait, S. (2008). “Full chain energy analysis of fuel ethanol from cane molasses in Thailand,” Applied Energy, 85, 722-734.
  11. Phisalaphong, M., Sriratana, N., Tanthapanichakoon, W.(2006).”Mathematical modeling to investigate temperature effecton kinetic parameters of ethanol fermentation”. Biochem. Eng. J., 28, 36–43.
  12. Phisalaphong, M., Budiraharjo, R., Bangrak, P., and Mongkolkajit, J. (2007). “Alginate-Loofa as carrier matrix for ethanol production,” J. Bioscience and Bioengineering, 104, 214-217.
  13. Verbelen, P.J., De Schutter, D.P., Delvaux, F., Verstrepen, K.J., and Delvaux, F.R. (2006). “Immobilized yeast cell systems for continuous fermentation applications,” Biotechnol. Lett., 28, 1515–1525.
  14. Yu, J., Zhang, X., and Tan, T. (2007). “An novel immobilization method of Saccharomyces cerevisiae to sorghum bagasse for ethanol production,” J. Biotechnol., 129, 415–420.



DOI: https://doi.org/10.22146/ajche.50089

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