2024-03-29T10:57:52Z
https://journal.ugm.ac.id/index/oai
oai:jurnal.ugm.ac.id:article/1823
2013-05-08T07:32:11Z
jfps:ART
"130326 2013 eng "
2339-0948
2089-7200
dc
The Role of Chemical Analysis in the Halal Authentication of Food and Pharmaceutical Products
Mursyidi, Achmad
Food and pharmaceutical products (drugs and cosmetics) are one of the essential human needs since the human civilization. It is an obligation for Muslim to consume and use the halal consumer goods. Currently, there are numerous food and pharmaceutical products available in the market supplied to consumers in which the actual contents of these products are not clear; consequently, the halal verification and authentication of these are highly required. Non-halal items commonly found are pig derivatives such as pork, lard, and gelatin as well as alcohol (khamr), blood, dead meats, and the allowed animals to be consumed which are not slaughtered according to Syariah law. One of the ways to authenticate the halalness of the food and pharmaceutical products is chemical analysis which is relied to find the specific markers present or absent in the products they contain. Currently, some analytical techniques have been proposed and continuously developed for the authentication of halal products such as Fourier transform infrared (FTIR), chromatography-based methods, differential scanning calorimetry, electronic nose, and DNA-based methods. However, these methods can not verify the non-halal items which are not physical, chemical, or biological in nature like dead animal. Consequently, another method should be performed, i.e. direct and monitoring the production step (from farm to table). Besides, to succed the verification and authentication of halal products, passing the legislation of “Halal products assurance” by Indonesian parliament is urgently needed.
Keyword: chemical analysis, food, pharmaceutical, halal authentication
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-08 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1823
Journal of Food and Pharmaceutical Sciences; Vol 1, No 1 (2013): J.Food Pharm.Sci (January-April)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1835
2013-05-08T07:32:11Z
jfps:ART
"130508 2013 eng "
2339-0948
2089-7200
dc
Analysis of Canola Oil in Virgin Coconut Oil Using FTIR Spectroscopy and Chemometrics
Man, Yaakob B. Che
Universiti Putra Malaysia, 43400 Serdang, Selangor
Rohman, abdul
Faculty of Pharmacy,
Gadjah Mada University, Yogyakarta http://acadstaff.ugm.ac.id/abdulrohman
Virgin coconut oil is a relative new oil comer in the industry of fats and oils, especially in ropical regions. In this study, we have investigated the possibility to use of Fourier transform infrared (FTIR) spectroscopy in combination with multivariate calibrations of partial least square (PLS) and principle component regression (PCR) as well as discriminant analysis (DA) for quantitative analysis and discrimination of canola oil (Ca-O) in virgin coconut oil (VCO). VCO, Ca-O, and the mixture of both oils was FTIR-spectroscopically scanned at mid infrared region (4,000 – 650 cm-1) using horizontal attenuated total reflectance. Several frequency regions as well as normal and derivative FTIR spectra were compared in order to obtain the best model for quantitative analysis. Finally, FTIR normal spectra at combined frequency regions of 1200-900 and 3027- 2985 cm-1 were selected for quantification of Ca-O due to their capabilities to provide the high correlation between actual and predicted values of CaO in VCO and low error values, either in calibration or validation models. Furthermore, DA was able to discriminate VCO and that adulterated with Ca-O. This result indicated that FTIR spectroscopy was possible to quantify and to discriminate Ca-O in VCO for authentication studies.
Keywords: FTIR spectroscopy, canola oil, virgin coconut oil, multivariate calibration, discriminant analysis
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-08 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1835
Journal of Food and Pharmaceutical Sciences; Vol 1, No 1 (2013): J.Food Pharm.Sci (January-April)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1837
2013-05-08T07:32:11Z
jfps:ART
"130508 2013 eng "
2339-0948
2089-7200
dc
Detection of nptII Gene and 35SCaMV Promoter in Tomatoes (Solanum lycopersicum L.)
Suratman, A.
Universitas Gadjah Mada, Yogyakarta
Ughude, J. O.
Universitas Gadjah Mada, Yogyakarta
., Sismindari
Faculty of Pharmacy, Gadjah Mada University, 55281 Yogyakarta http://sismindari.staff.ugm.ac.id/?page_id=18
The detection of nptII (kanamycin resistance) as a transgenic marker gene and 35SCaMV as promoter in tomatoes has been carried out. DNA from tomatoes samples was isolated using PureLinkTM plant total DNA purification kit. The purity of DNA samples was estimated using UV-Vis spectrophotometry at 260 nm and 280 nm. They gave an absorbance ratio (A260/A280) of 1.74-1.79 which indicated its purities. The quality of the DNA was confirmed by a clear and thick band, as analyzed in 0.8% agarose gel electrophoresis. In order to identify the transgenic tomatoes, a 786-bp fragment of the nptII gene and a 86-bp fragment of 35SCaMV promoter were amplified using polymerase chain reaction (PCR). PCR reaction was prepared at optimum condition, namely annealing temperature at 56°C and 55°C for nptII gene and 35SCaMV promoter, respectively and 300 ng of DNA template. The PCR results were visualized on 2% agarose gel electrophoresis. The results showed that one of three tomatoes (code ST2) contains 35SCaMV promoter and no tomatoes contain nptII gene, indicating that ST2 is transgenic tomato.
Key words: tomatoes, PCR, nptII, 35SCaMV
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-08 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1837
Journal of Food and Pharmaceutical Sciences; Vol 1, No 1 (2013): J.Food Pharm.Sci (January-April)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1838
2013-05-08T07:42:30Z
jfps:ART
"130508 2013 eng "
2339-0948
2089-7200
dc
Effect of Taurine on The Respiratory System of Rats
E.M, Ammer
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, 35516, Mansoura, Egypt
A.A., Shaaban
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, 35516, Mansoura, Egypt
H.A., Ghonem
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, 35516, Mansoura, Egypt
H.A., Elkashef
Effect of Taurine on The Respiratory System of Rats
The present study was designed to investigate the effect of taurine on isolated trachea and pulmonary artery of rats and the possible mechanism(s) of action. The possible antioxidant effect of taurine was also studied by measuring its protective effect against cyclophosphamide induced lung injuiry. Taurine produced a concentration dependent relaxation in the isolated tracheal strips and pulmonary arterial rings precontracted by serotonin (2x10-4
mM). The relaxing effect of taurine was not influenced by pretreatment
with nitric oxide synthase inhibitor (L-NAME) , cysteinyl leukotreines receptor 1 blocker (montelukast) , H1 receptor blocker (chlorpheniramine) , β-adrenoceptor blocker (propranolol), potassium channel blocker (amiodarone) , cyclo-oxygenase inhibitor
(indomethacin) or muscarinic receptor blocker (atropine). Preincubation with adenosine receptor blocker (aminophylline) significantly potentiated the relaxing effect of taurine in the tracheal strips and pulmonary arterial rings. Cyclophosphamide (CYP, 150 mg/kg) administerated i.p. in a single dose was used to produce lung injuiry in rats. CYP caused marked increase in lung lipid peroxides (MDA) and decrease in lung reduced glutathione (GSH). Administration of taurine (1% in drinking water) starting 7 days before CYP and continuing throughout the duration of the experiment (24 hours) improved significantly
the lung GSH and MDA. It can be concluded that taurine relaxes precontracted rat tracheal strips and pulmonary arterial rings probably by direct effect on the smooth muscles. Also, the observed antioxidant activity of taurine which may contribute to its relaxant effect
suggesting the usefulness of turine in pulmonary hypertension.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-08 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1838
Journal of Food and Pharmaceutical Sciences; Vol 1, No 2 (2013): J. Food Pharm. Sci (May-August)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1839
2013-05-08T07:32:11Z
jfps:ART
"130508 2013 eng "
2339-0948
2089-7200
dc
The Effect of Size Reduction and Preparation Duration on The Antioxidant Activity of White Saffron (Curcuma mangga Val.)
Pujimulyani, Dwiyati
Faculty of Agroindustry, Mercu Buana University, Yogyakarta, Indonesia
Raharjo, Sri
Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta, Indonesia
Marsono, Y
Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta, Indonesia
Santoso, Umar
Faculty of Agricultural Technology, Gadjah Mada University, Yogyakarta, Indonesia
Food processing is usually conducted through preparation stages such as peeling, slicing, or cutting. The purpose of this research was to determine the correlation between the antioxidant activity and the total phenolic compounds of white saffron as affected by different level of size reduction and the duration of rhizome preparation at room temperature. White saffron rhizomes were peeled, washed, and cut into 1x1x1 cm3. The whole white saffron was sliced diagonally at thickness of 4 x 2 mm, and grated. The antioxidant activity of the white saffron was determined by radical scavenging activity using 2-2-diphenyl-1-picrylhydrazyl (DPPH) radical and by ferric reducing antioxidant power FRAP) method. The total phenol and total flavonoid contents were also determined at 2, 4, and 6 hours, respectively. The result showed that the antioxidant activities, total phenolic and flavonoid contents due to the preparation of whole white saffron for 6 hour are not statistically different compared to those of fresh white saffron. The preparation of white saffron sliced at 2 mm and grated for 2 hour showed a significant decrease in the antioxidant activities, compared to those of fresh white saffron.
Keywords: white saffron, preparation duration, antioxidant activity, total phenol
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-08 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1839
Journal of Food and Pharmaceutical Sciences; Vol 1, No 1 (2013): J.Food Pharm.Sci (January-April)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1840
2013-05-08T07:42:30Z
jfps:ART
"130508 2013 eng "
2339-0948
2089-7200
dc
Some Physico-chemical Properties of Red Fruit Oil (Pandanus Conoideus Lam) from Hexane and Chloroform Fractions
Arumsari, Novita Inar
Faculty of Pharmacy, Gadjah Mada University http://acadstaff.ugm.ac.id/abdulrohman
Riyanto, Sugeng
Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281 Indonesia
Rohman, Abdul
Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281 Indonesia
As functional food oil, red fruit (Pandanus conoideus Lam) oil has been believed by local community to threat several degenerative diseases like cancer. Red fruit oil (RFO) has commanded high price value in Indonesian market. Therefore, the objective of the present
study was to investigate several physico-chemical properties of RFO. Some parameters of RFO obtained from hexane and chloroform fractions have been evaluated. Such parameters include acid value, saponification value, anisidine value, conjugated dienes and trienes as well as p-anisidine value. Besides, absorptivity coefficient, fatty acid composition, and volatile compounds were also determined. Acid value and saponification value of RFO from chloroform fraction were higher than those in RFO from hexane fraction. In addition, RFO in hexane fraction has the higher iodine and anisidine values than that in chloroform fraction. Hexane is the best solvent to be used for analysis of RFO, as indicated by the highest absorptivity coefficient of RFO in hexane. The main fatty acid composed of RFO was oleic acid followed with palmitic acid. The main volatile compound present in RFO of hexane and chloroform fractions was 9-octadecenoic acid accounting of 41.57 % and 65.06 %, respectively.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-08 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1840
Journal of Food and Pharmaceutical Sciences; Vol 1, No 2 (2013): J. Food Pharm. Sci (May-August)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1841
2013-05-08T07:32:11Z
jfps:ART
"130508 2013 eng "
2339-0948
2089-7200
dc
Changes in Gibberellic Acid (GA3) Content in Oryza sativa Due to Paclobutrazol Treatment
Syahputra, Bambang S.A.
Faculty of Agriculture, Pancabudi University, Medan, North Sumatera, Indonesia
Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang
Sinniah, Uma Rani
Department of Crop Science, Universiti Putra Malaysia, 43400 UPM Serdang
SR, Syed Omar
Department of Land Management, Faculty of Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang.
Ismail, Mohd. Razi
Institute of Tropical Agriculture, Universiti Putra Malaysia, 43400 UPM Serdang.
The objective of this study was to determine the level of plant hormone gibberellic acid (GA3) in paddy due to the treatment of Paclobutrazol (PBZ) treatment using high performance liquid chromatography (HPLC) with UV-vis detection at 208 nm. The separation was achieved using reversed column Crestpak C18 (150 mm x 4.6 mm i.d; 5 µm) at 30 ± 1° C using mobile phase of acetonitrile-water (30:70%; v/v), pH 6.80. The treatment of PBZ with different concentration of 100, 200, 400, and 600 mg/L reduced the concentration of GA3 in paddy. The level of GA3 in paddy treated with 100 mg/L of PBZ did not show significant difference from untreated one. However, the level of GA3 in paddy treated with other concentrations (200, 400, and 600 mg/L) of PBZ was significantly different (P < 0.05) from untreated paddy.
Keywords: analysis, gibberellic acid, paclobutrazol, HPLC
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-08 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1841
Journal of Food and Pharmaceutical Sciences; Vol 1, No 1 (2013): J.Food Pharm.Sci (January-April)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1842
2013-05-08T07:44:29Z
jfps:ART
"130508 2013 eng "
2339-0948
2089-7200
dc
Myocardial Depression and Inhibition of Positive Inotropic Effect of Digoxin by Rosiglitazone
Said, Shehta A.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, 35516, Mansoura, Egypt
Nader, Manar A.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, 35516, Mansoura, Egypt
This study was taken to investigate the effect of rosiglitazone (RGN) on the contractility of
the isolated rat atrium as well as its possible inhibitory effect on positive inotropic effect of digoxin. RGN significantly increased the dose of digoxin required to produce cardiac arrest in anesthetized rats. RGN (10, 20, 40, 60, 80 & 100 µM) produced concentration dependant depressant effect on atrial contractility in both diabetic and non-diabetic animals with the depressant effect was more pronounced in diabetic rats than in non-diabetic ones. RGN also inhibited the positive inotropic effect of digoxin (0.1, 0.3 & 1µM) in isolated rat right atrium treated with 4 mg/kg orally daily for 21 days. Moreover, RGN also produced significant increase in serum K+
and decrease in Na+ levels in rats. These results indicate that RGN has a negative inotropic effect on the heart especially in diabetics and it inhibits the positive inotropic effect of digoxin.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-08 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1842
Journal of Food and Pharmaceutical Sciences; Vol 1, No 2 (2013): J. Food Pharm. Sci (May-August)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1843
2013-05-08T07:46:00Z
jfps:ART
"130508 2013 eng "
2339-0948
2089-7200
dc
Analysis of Melamine Using Electronic Nose, Gas Chromatography-Time of Flight - Mass Spectrometry and High Performance Liquid Chromatography
Rohman., Abdul
Faculty of Pharmacy, Gadjah Mada University http://acadstaff.ugm.ac.id/abdulrohman
Che Man, Yaakob B.
Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
Hafidz, R. M.
Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia
An electronic nose (E-Nose) has been used as rapid analytical technique for melamine detection in food samples. The parameter used is melamine retention index (MRI) in E-Nose chromatogram. The value of MRI was also confirmed using two dimensional gas
chromatography-time of flight-mass spectrometry (GCxGC-TOF-MS). The value of MRI using E-Nose and GCxGC-TOF-MS was nearly close i.e 1486 and 1540 respectively. HPLC has been used for quantitative analysis of melamine in some milk products suspected to contain melamine using column Nova-Pak® C18;(150 x 3.9 mm i.d; 4 µm) at 30°C. The mobile phase
was 5 mmol L−1 NaH2PO4 delivered isocratically at flow rate of 1 mLmin-1 . Melamine was linear at concentration range of 0.0625 – 50.0 µg/ml with coefficient of determination (R2 ) of 0.999.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-08 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1843
Journal of Food and Pharmaceutical Sciences; Vol 1, No 2 (2013): J. Food Pharm. Sci (May-August)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1844
2013-05-08T07:47:41Z
jfps:ART
"130508 2013 eng "
2339-0948
2089-7200
dc
Extraction and Stability Test of Anthocyanin from Buni Fruits (Antidesma Bunius L) as an Alternative Natural and Safe Food Colorants
Amalia, Fera
Faculty of Pharmacy, Gadjah Mada University
Afnani, Galih Nur
Faculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281, Indonesia
Synthetic dyes have widely been usedin food products. However, the safety issues regarding the use of synthetic dyes arise. Buni (Antidesma bunius) which contains anthocyanins can be an alternative of synthetic dyes. Anthocyanins are red, blue, or violet natural colorants from many types of plants. The aim of the study was to determine the
best extraction process and to evaluate the stability of anthocyanins extracted from the Buni fruits. Different combinations of the solvents namely aquadest, ethanol, hydrocloride acid, and citric acid were used to extract the anthocyanins. The most appropriate combination of solvent was determined by the analysis of total anthocyanins content and
color intensity using UV-Visible spectrophotometer. The stability of anthocyanins was evaluated using different pH values and temperature. Ethanol at 70% and citric acid at 3% was the most appropriate combination for the extraction with the highest total concentration of anthocyanin (0.58%) and color intensity (0.324). The stability of the anthocyanins from Buni was affected by the value of pH, which the most stable condition
was set at pH values of 4-5.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-08 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1844
Journal of Food and Pharmaceutical Sciences; Vol 1, No 2 (2013): J. Food Pharm. Sci (May-August)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1845
2013-05-09T06:32:42Z
jfps:ART
"130509 2013 eng "
2339-0948
2089-7200
dc
Review Article: Analysis of Emulsifier in Food Using Chromatographic Techniques
Rohman., Abdul
Faculty of Pharmacy, Gadjah Mada University http://acadstaff.ugm.ac.id/abdulrohman
Che Man, Yaakob B
Halal Products Research Institute, Universiti Putra Malaysia, 43400 UPM, Serdang, Selangor, Malaysia.
Noviana, Eka
Emulsifiers so far are important class of additives used in food products. Food industries and regulatory authorities have striven for the continuous development of analytical methods to determine the emulsifiers in foods. Chromatography is one of the powerful analytical techniques used in the analysis of food components due to its capability for the separation and quantitative analyses of emulsifiers. This article describes some chromatographic techniques, namely gas chromatography (GC), high performance liquid chromatography (HPLC), supercritical fluid chromatography (SFC), and planar chromatography for detection and quantification of emulsifiers in food. Sample preparation involved in the analysis of emulsifiers has been also highlighted.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-09 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1845
Journal of Food and Pharmaceutical Sciences; Vol 1, No 3 (2013): J. Food Pharm. Sci (September-December)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1846
2013-05-09T06:32:42Z
jfps:ART
"130509 2013 eng "
2339-0948
2089-7200
dc
Some Physicochemical Properties of Iranian Native Barberry Fruits (abi and poloei): Berberis integerrima and Berberis vulgaris
Ardestani, Samira Berenji
Department of Food Technology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
Sahari, Mohammad Ali
Department of Food Technology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
Barzegar, Mohsen
Abbasi, Soleiman
Owing to a combination of medicinal and nutritional values (functional food); barberry plants are of interest to researchers. Barberry is a valuable native Iranian plant which is cultivated as abi and poloei varieties (Berberis integerrima - B. vulgaris). Amounts of ash (1.0671- 0.7363%), fat (2.9674 - 0.6173%), fiber (12.1059 - 2.6222%), protein (0.5043 - 0.1200%), reducing sugars (8.8426 - 6.6671%), total sugar (13.8573 - 9.4827%) and pH (3.160 -3.060) were higher in integerrima whereas moisture content (56.27 - 75.01%), Brix (11.1666 - 17.3333) and colour indexes (L*: 16.8500 - 20.8200, a*: 5.6866 - 34.8400 and b*: -1.0066 - 18.9066) were higher in vulgaris. The amounts of P, Zn, Fe, Na and K (ICP technique) in vulgaris were significantly higher than those of integerrima (whole and seedless fruits) and the highest amounts of Mn, Mg, and Cu, Ca were recorded in whole and seedless fruits of integerrima, respectively. Total phenolic and total anthocyanin contents were 8530 and 183.51 mg in 100 g fresh fruits of B. integerrima (as major anthocyanin delphinidin-3-glucoside equivalent), 3450 and 14.8 mg in 100 g fresh fruits of B. vulgaris (as major anthocyanin pelargonidin-3-glucoside equivalent), respectively.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-09 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1846
Journal of Food and Pharmaceutical Sciences; Vol 1, No 3 (2013): J. Food Pharm. Sci (September-December)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1847
2013-05-09T06:32:42Z
jfps:ART
"130509 2013 eng "
2339-0948
2089-7200
dc
Differentiation of Bovine and Porcine Gelatin Based on Spectroscopic and Electrophoretic Analysis
Hermanto, Sandra
Department of Chemistry, Faculty of Science and Technology,, Syarif Hidayatullah Jakarta State Islamic University, Indonesia
Sumarlin, La Ode
Fatimah, Widya
This study was conducted to explore the differentiation of bovine and porcine gelatins before and after pepsin hydrolysis based on peptide pattern from spectroscopic and electrophoretic analysis due to development of the halal food products analysis. In this study, pepsin (EC 3.4.23.1) was used to hydrolyze the two sources of gelatin with consideration to its ability to digest up to 20% of ingested amide bonds by cleaving preferentially after the N-terminal of aromatic amino acids such as phenylalanine, tryptophan, and tyrosine. In this study, we expect to produce the fragment of gelatins with differentiation in relative molecular weights. Gelatins fragments then analyzed by UV-Vis and FTIR spectroscopy to characterize the functional groups on each source of gelatins, followed by SDS-PAGE (Sodium Duodecylsulphate Polyacrylamide Gel Electrophoresis) to identify the molecular weight of the resulting fragments. In UV-Vis spectroscopy, both gelatin source before and after hydrolysis had different absorbance at 229 nm and 240 nm showing the proportion of C=O amida and differences in two-dimensional conformation of the peptide. In terms of FTIR spectra, both gelatin have wavenumber at 3300-3400 cm-1 (NH stretching), 1600 cm-1 (C=O stretching, amida), 1500 cm-1 (C-N stretching), and at 620-767 cm-1 (OCN bending). This indicates that the relative amino acid compositions from two sources of gelatins were relatively different. In contrast, SDS-PAGE analysis does not give a real differentiation, except for porcine gelatin, that fragments which on 2 hour incubation show two peptide fragments with molecular sizes below 36,2 kDa and 28.6 kDa.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-09 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1847
Journal of Food and Pharmaceutical Sciences; Vol 1, No 3 (2013): J. Food Pharm. Sci (September-December)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1848
2013-05-09T06:32:42Z
jfps:ART
"130509 2013 eng "
2339-0948
2089-7200
dc
Physico-Chemical, Mineral, Amino Acid Composition, in Vitro Antioxidant Activity and Sorption Isotherm of Pithecellobium dulce L. Seed Protein Flour
Rao, Galla Narsing
Council of Scientific and Industrial Research - Central Food Technological Research Institute - Resource Centre, Habshiguda, Uppal Road, Hyderabad-500 007, India
Pithecellobium dulce L. seed protein flour (PSPF) was evaluated for the chemical, mineral, amino acid composition, in vitro antioxidant activity and sorption isotherm. Protein content was found to be 39.22% PSPF. Calcium (48 mg) and phosphorus (542 mg/100 g) were observed in major quantities. Major amino acids were determined as glutamic acid, arginine, aspartic acid, lysine, valine, threonine and leucine. The ratio of essential to nonessential amino acids was observed to be 0.61. Essential amino acids were higher than the reported amounts for 70 kg person (100 g seed protein flour) as per FAO/WHO/UNU requirements. DPPH, Inhibition (25-73%) and ferric reducing power OD (0.136-0.523) are increased with increasing concentration of PSPF from 3-15 mg. SDS-PAGE of PSM and PSPF showed similar polypeptides with molecular weights from 205 kDa to 12 kDa. The IMC (10.34%) of PSPF, which equilibrated at 69% RH, indicated the non-hygroscopic nature.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-09 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1848
Journal of Food and Pharmaceutical Sciences; Vol 1, No 3 (2013): J. Food Pharm. Sci (September-December)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1849
2013-05-10T02:38:54Z
jfps:ART
"130510 2013 eng "
2339-0948
2089-7200
dc
Estimation of Naturally Produced Water-Soluble Vitamins in Different Asavas and Arishtas Using Liquid Chromatography
Singh, Nikhil Kumar
Sharma, Alok
Dabur Research & Development Centre, Dabur India Limited, Ghaziabad, India
Sharma, Meena
Singh, Rahul
Dabur Research & Development Centre, Dabur India Limited, Ghaziabad, India
Katiyar, Chandrakant
Self fermented polyherbal Ayurvedic preparations are called Asavas and Arishtas and are efficacious classical preparations. Insignificant efforts have been made to delve deep into scientific rationale of this ancient dosage form of Ayurveda. An investigative analysis using reversed phase chromatography was conducted to evaluate and estimate the presence of water-soluble vitamins in seven Asavas and Arishtas namely Drakshasava, Ashokarishta, Dashmulrishta, Khadirarishta, Lauhasava, Arjunarishta and Ashwagandharishta. The study revealed that these preparations contained water soluble vitamins B1, B2, B3 & B6 in significant concentrations. Reversed phase high performance liquid chromatography was carried out on an Agilent 1200 DAD system. Separation was carried out on an Inertsil ODS 3 V column (250 mm x 4.6 mm, 5 µm) at wavelength of 265 nm.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-09 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1849
Journal of Food and Pharmaceutical Sciences; Vol 1, No 3 (2013): J. Food Pharm. Sci (September-December)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1850
2013-05-12T11:31:14Z
jfps:ART
"130510 2013 eng "
2339-0948
2089-7200
dc
Differentiation of Bovine and Porcine Gelatins in Soft Candy Based on Amino Acid Profiles and Chemometrics
Raraswati, Mita Amalia
Faculty of Pharmacy, Gadjah Mada University http://acadstaff.ugm.ac.id/abdulrohman
Triyana, Kuwat
., Triwahyudi
Rohman, Abdul
Research center of Halal Products, Gadjah Mada University, Yogyakarta 55281 Indonesia
Gelatin is widely used in some food products, including soft candy (one of food products preferred by children). Most of the gelatin available in the market derived from pigs. Some religions like Islam and Jews prohibited their followers to consume any food products containing pig derivatives, including porcine gelatin. Therefore, it is necessary to develop some rapid and reliable methods for detection of porcine gelatin in soft candy. The purpose of this study was to differentiate and classification the gelatin sources (porcine or bovine) in soft candy based on amino acid profiles combined with chemometrics of principal component analysis (PCA). Separation and determination of amino acid was conducted by reversed-phase HPLC using a fluorescent detector, after being derivatized with ortho-phtalaldehyde in 2-mercaptoethanol (OPA/2-MCE). Parameters of peak height percentage of each amino acids from each sample were analyzed by PCA. Based on PC1 and PC2, porcine and bovine gelatins in soft candy could be apparently distinguished.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-10 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1850
Journal of Food and Pharmaceutical Sciences; Vol 2, No 1 (2014): J. Food Pharm. Sci (January-April)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1857
2013-05-12T11:31:14Z
jfps:ART
"130512 2013 eng "
2339-0948
2089-7200
dc
Paracetamol Supplementation Does Not Alter The Antitumor Activity and Lung Toxicity of Bleomycin
Suddek, Ghada M.
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Egypt.
Salem, Hatem A.
Badary, Osama A.
Gameil, Nariman M.
El-Kashef, Hassan A.
Bleomycin (BLM) is well known by its antitumor activity both in vitro and in vivo. However, pulmonary fibrosis has been considered the dose limiting toxicity of the drug. Hyperpyrexia following injection of BLM was reported thus, paracetamol is sometimes administered with BLM as antipyretic drug. Actually, paracetamol was found to interfere with cytotoxicity of some drugs. This study was conducted to investigate the effect of paracetamol administration on the antitumor and lung toxicity of BLM. The antitumor activity was evaluated both in vitro and in vivo using Ehrlich ascites carcinoma (EAC) cells. Paracetamol did not alter the antitumor effect of BLM in vitro or in vivo. The lung toxicity of BLM was evidenced by decrease in the body weight, increase in the lung/body weight ratio, decrease in the response of pulmonary arterial rings to 5-hydroxytryptamine (5-HT) and increase in the contractility of tracheal smooth muscles induced by acetylcholine (ACh). The toxicity was also confirmed biochemically by marked increases in hydroxyproline and lipid peroxidation in rat lung and the decrease in reduced glutathione (GSH) level. Pretreatment with paracetamol did not significantly change lipid peroxidation, GSH level, percent survival of rats or the response of pulmonary arterial rings and tracheal smooth muscles to 5-HT and ACh respectively. The results of the present study indicated that paracetamol neither modified the antitumor effect of BLM nor changed drug-induced lung toxicity.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-10 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1857
Journal of Food and Pharmaceutical Sciences; Vol 2, No 1 (2014): J. Food Pharm. Sci (January-April)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1858
2013-05-12T11:31:14Z
jfps:ART
"130512 2013 eng "
2339-0948
2089-7200
dc
Physico-Chemical, Functional and Antioxidant Properties of Roe Protein Concentrates from Cyprinus carpio and Epinephelus tauvina
Rao, Galla Narsing
Council of Scientific and Industrial Research, Central Food Technological Research Institute, Resource Centre, Habshiguda, Uppal Road, Hyderabad 500 007, India
Roe protein concentrates prepared from Cyprinus carpio (CRPC) and Epinephelus tauvina (ERPC) were investigated for physico-chemical characteristics, functional properties and antioxidant activity. Cyprinus and Epinephelus roes yielded 19.5% and 21.5% of protein concentrates possessing 70.71% and 85.9% protein, respectively. Moisture sorption isotherms of roe protein concentrates indicated the non-hygroscopic nature of CRPC with initial moisture content (IMC) of 8%, which equilibrated at 63% RH and hygroscopic nature of ERPC with IMC of 4.9%, which equilibrated at 42% RH. Water absorption capacity, oil absorption capacity, foam capacity and lower foam stability and emulsifying capacity were found in ERPC than in CRPC respectively. Maximum solubility of protein was 17.79% and 16.89% at pH 12, observed in CRPC and ERPC respectively. Higher buffer capacity was observed in both roe protein concentrates in alkali medium. Antioxidant activity determined by the DPPH radical scavenging activity was higher, and ferric reducing power was observed to be lower in ERPC.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-10 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1858
Journal of Food and Pharmaceutical Sciences; Vol 2, No 1 (2014): J. Food Pharm. Sci (January-April)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1859
2013-05-12T11:31:14Z
jfps:ART
"130512 2013 eng "
2339-0948
2089-7200
dc
Analytical Method Validation of Selected Organochlorine Pesticides in Fortified Rice Using Gas Chromatography with Electron Capture Detector
Wardani, Mutia Kusuma
Faculty of Pharmacy, Gadjah Mada University
Sutriyanto, Bambang
Lukitaningsih, Endang
Rohman, Abdul
Gas chromatography with electron capture detector (GC-ECD) has been validated for simultaneous determination of selected organochlorine pesticides namely heptachlor, endosulfan, dieldrin, dan p,p’-dichlorodiphenyltrichloroethane (p,p’-DDT) using aldrin as standard internal. Some parameters for analytical method validation were evaluated, i.e. linearity, precision, sensitivity expressed with limit of detection and limit of quantification, and recovery. GC-ECD was linear for determination of these pesticides with r values ≥ 0.99. The values of LOD obtained were 2.50, 2.97, 1.61, and 2.54 ng/mL for heptachlor, endosulphan, dieldrin, and p,p’-DDT, respectively. Furthermore, the relative standard deviation (RSD) values obtained for the assessment of analytical precision were lower than those required by Horwitz. Finally, the used GC-ECD method was accurate for determination of these organochlorine psticides in fortified rice samples which were indicated by the acceptable recovery percentages.
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-10 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1859
Journal of Food and Pharmaceutical Sciences; Vol 2, No 1 (2014): J. Food Pharm. Sci (January-April)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/1860
2013-05-12T11:31:14Z
jfps:ART
"130512 2013 eng "
2339-0948
2089-7200
dc
Differentiation of Lard from Other Edible Fats by Gas Chromatography-Flame Ionisation Detector (GC-FID) and Chemometrics
Dahimi, Omar
Institute of Halal Research and Management (IHRAM), Universiti Sains Islam Malaysia, 71800, Nilai, Negeri Sembilan, Malaysia http://acadstaff.ugm.ac.id/abdulrohman
Hassan, Mohd. Sukri
Rahim, Alina Abdul
Institute of Halal Research and Management (IHRAM), Universiti Sains Islam Malaysia, 71800, Nilai, Negeri Sembilan, Malaysia
Abdulkarim, Sabo Mohammed
Mashitoh A., Siti
The presence of lard or its derivatives in any food products is a serious religious issue among Muslim and Judaism. Thus, the objective of this study was to investigate the use of gas chromatography with flame ionisation detector (GC-FID) coupled with chemometrics techniques such as Principle Components Analysis (PCA) and K-mean cluster analysis to differentiate lard adulteration at very low concentrations in beef and chicken fats. The measurements were made from the pure lard, beef tallow, pure chicken fat; and beef tallow (BT), chicken fat (CF) adulterated with different concentrations of lard (0.5%-10% in BT and CF). The data were first scaled into standardisation before PCA is performed to each of the scaled data using Unscrambler software. The Scores plots and loadings plots of each scaled data were compared and studied. The results showed that lard contains higher fatty acid (FA) of C18: 2cis and low C16:0 FA, but oppositely for beef tallow and chicken fat. The amount of C4:0, C14:0, and C18:0 FAs are approximately similar for all fats. Others FAs are small in amount and nearly similar for both. Additionally, PCA was able to significantly identify lard, beef fat, chicken fat and the mixtures of lard and beef tallow, lard and chicken fat, even at lower concentration level (0.5 % lard-99.5% beef tallow / chicken fat (w/w). K-mean cluster only able to classify the pure lard (LD), pure chicken fat (CF) and pure beef tallow (BT)
Fakultas Farmasi, Universitas Gadjah Mada
2013-05-10 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/1860
Journal of Food and Pharmaceutical Sciences; Vol 2, No 1 (2014): J. Food Pharm. Sci (January-April)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/2347
2014-06-16T01:13:46Z
jfps:ART
"130730 2013 eng "
2339-0948
2089-7200
dc
Antioxidant Activity of Stevia(Stevia rebaudianaL.) Leaf Powder and A Commercial Stevioside Powder
Rao, Galla Narsing
CSIR - Central Food Technological Research Institute, Resource Centre, Habshiguda, Uppal Road, Hyderabad - 500 007, India http://acadstaff.ugm.ac.id/abdulrohman
Stevia leaf powder (SLP) and a commercial stevioside powder (CSP) were analysed for their polyphenol content, isothermal sorption behavior and their antioxidant activity was evaluated by DPPH radical scavenging activity, ferric reducing power and ABTS assay. Polyphenol contents were higher (5.6%) in SLP than in CSP (2.3%). It was observed that the SLP was non-hygroscopic and CSP was hygroscopic in nature as seen from sorption isotherms. The equilibrium relative humidity of SLP and CSP respectively was 62 and 24%. The antioxidant activity of the methanolic extracts were determined and DPPH radical scavenging activity for SLP showed 30.33% for 20 µg/ml and 52.46% inhibition at 100 µg/ml, which were slightly higher compared to CSP stevioside, which showed 29.5% and 47.64% inhibition for similar concentrations.Higher ferric reducing power was seen in CSP (0.632) than SLP (0.166) for 100 µg.The antioxidant activity measured by ABTS assay also indicated higher activity (37.5% inhibition for10 µg/ml) for SLP than CSP (39.66% for 40 µg/ml).
Fakultas Farmasi, Universitas Gadjah Mada
2014-06-11 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/2347
Journal of Food and Pharmaceutical Sciences; Vol 2, No 2 (2014): J. Food Pharm. Sci (May-August)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/2432
2014-06-16T01:13:46Z
jfps:ART
"131015 2013 eng "
2339-0948
2089-7200
dc
Protein Profilesof Beef (Bos indicus), Pork (Sus domesticus),and SausagesBy Using SDS-PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis) Method
Zilhadia, Zilhadia
SyarifHidayatullah Islamic State University, Jakarta, Indonesia
A research has been done to analyze the protein profile in fresh beef, fresh pork, and 10 beef sausage by using SDS PAGE (Sodium Dodecyl Sulphate Polyacrylamide Gel Electrophoresis) with 2 plate gel electrophoresis. From this research, we found several protein bands that become distinctive protein bands. On raw beef protein we found three bands that are not found in pork. They are protein band with molecular weight (MW) of 144,54 kDa, 81,28 kDa and 58,88 kDa respectively. On the raw pork, we found 5 protein bands that are not found in raw beef, namely protein bands with MW 154,88 kDa; 146,55 kDa; 83,18 kDa; 69,18 kDa and 61,66 kDa. There is a band on pork protein found on the second plate on MW gel is 69,18 kDa. Whereas in 10 samples of beef sausages we did not found any specific protein bands. This is presumably due to the difference in manufacturing process performed by the manufacturer.
Fakultas Farmasi, Universitas Gadjah Mada
2014-06-11 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/2432
Journal of Food and Pharmaceutical Sciences; Vol 2, No 2 (2014): J. Food Pharm. Sci (May-August)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/4844
2014-06-16T01:13:46Z
jfps:ART
2339-0948
2089-7200
dc
Mitigating Role of Quercetin Against Cyclophosphamide-Induced Lung Injury in Rats
A. Asry, Nora
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Mansoura University, Egypt.
Quercetin (Qur), a polyphenolic flavonoid compound present in large amounts in vegetables and fruits, plays important roles in human health through its antioxidant activity. This study was conducted to investigate the possible modulatory effect of Qur against cyclophosphamide (CP)-induced lung oxidative damage and to highlight the underlying mechanisms of such effect. Male Sprague-Dawely rats were divided into four groups. Group I was control. Group II received Qur (100 mg/kg/d. p.o.) for 14 consecutive days. Group III was injected once with CP (150 mg/kg, i.p.). Group IV received Qur for 7 consecutive days, before and after CP injection.
A single i.p. injection of CP markedly increased the level of serum biomarkers; total protein, LDH. Cyclophosphamide significantly increased the lung content of lipid peroxides and decreased levels of reduced glutathione. Treatment of rats with Qur for 7 days prior to and 7 days after cyclophosphamide significantly ameliorated the alterations in lung and serum biomarkers associated with inflammatory reactions. Moreover, Qur attenuated the secretion of pro-inflammatory cytokine, TNF-α in rat serum. In addition, Qur slightly ameliorated CP-induced histopathological changes in lung tissue.
Our results suggest that Qur produces a protective effect against CP-induced lung injury and suggest a role of oxidative stress and inflammation in the pathogenesis.
Fakultas Farmasi, Universitas Gadjah Mada
2014-06-11 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/4844
Journal of Food and Pharmaceutical Sciences; Vol 2, No 2 (2014): J. Food Pharm. Sci (May-August)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/4845
2014-06-16T01:13:46Z
jfps:ART
2339-0948
2089-7200
dc
The Antiradical Activity of Insoluble Water Suji (Pleomele angustifolia N.E. Brown) Leaf Extract and Its Application as Natural Colorant in Bread product
Jokopriyambodo, Wahyu
Currently, there are some synthetic colouring agents present in food products. The synthetic colouring agents are supposed to give deleterious effect to human health; therefore, natural coloring agents derived from plant is continuously explored to replace the synthetic ones. Suji (Pleomele angustifolia) leaf is one of the potential plant to be used as natural coloring agents. The aim of this study is to explore the natural colour potency and antiradical activity of insoluble water extract of suji leaf from three different regions. Furthermore, green components obtained from suji leaf extract (SLE) was used for coloring food product (bread product), and bread added with extract was subjected to preference test, namely color and taste preferences by trained panelist. The result showed that region and maturity level affect the intensity of green components obtained from SLE. The highest level of green components (2.57 %) among three regions evaluated was observed from Jumantono. The water insoluble extract of suji leaf can also make coloring effects toward bread and exhibits low radical scavenging against 2,2-diphenyl-1-picrylhidrazyl. The preference test against color and taste of bread added with synthetic coloring agents and mixed with SLE showed that the coloured bread is less preferred than that without coloring agent.
Fakultas Farmasi, Universitas Gadjah Mada
2014-06-11 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/4845
Journal of Food and Pharmaceutical Sciences; Vol 2, No 2 (2014): J. Food Pharm. Sci (May-August)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/4865
2014-06-16T01:13:46Z
jfps:ART
2339-0948
2089-7200
dc
Control of Aspergillus flavus Growth in Tomato Paste by Cinnamomum zeylanicum and Origanum vulgare L. Essential Oils
Kalantary, F
This study was conducted to evaluate the antifungal activities of cinnamon (Cinnamomum zeylanicum) and oregano (Origanum vulgare L.) essential oils against Aspergillus flavus in culture media and tomato paste. The chemical compositions of the essential oils were determined by Gas Chromatography-Mass Spectroscopy (GC-MS). Trans- cinnamaldehyde was found to be the main constituent of Cinnamomum zeylanicum essential oil (CZEO), followed by methyl eugenol, δ- cadinene and γ- cadinene. The major components of Origanum vulgare L. essential oil (OVEO) were limonene, caryophyllene oxide, α-ionone, germacrene– D, γ- terpinene, β- pinene and terpinene-4-ol. For evaluating antifungal activities of CZEO and OVEO, A. flavus PTCC: 5006, was inoculated in Sabouraud Dextrose Broth (SDB) and tomato paste, then 0, 50, 100, 200, 300, 400, 500 and 600 ppm of essential oils were added to each sample and incubated at 25±0.5oC for 30 and 60 days, respectively. The antifungal activity was measured by Agar Dilution method. The EOs at all tested concentrations had inhibitory effect against A. flavus growth. 200 ppm of CZEO and 500 ppm of OVEO completely inhibited A. flavus growth in culture media, while in tomato paste 300 ppm of CZEO and 200 ppm of OVEO had the same effect. Test panel evaluations were carried out in tomato ketchup base and samples with 100 and 200 ppm CZEO were accepted by panelists. The results may suggest the potential replacement of antifungal chemicals by CZEO as natural inhibitor to control A. flavus growth in tomato paste.
Fakultas Farmasi, Universitas Gadjah Mada
2014-06-11 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/4865
Journal of Food and Pharmaceutical Sciences; Vol 2, No 2 (2014): J. Food Pharm. Sci (May-August)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/4866
2014-06-16T01:13:46Z
jfps:ART
2339-0948
2089-7200
dc
Influence of Drying on the quality of Ashwagandha (Withania somnifera)
Agrawal, A
Department of Crop and Herbal Physiology College of Agriculture Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur (M.P.), India
An experiment was carried out to adjudge the effect of different drying techniques on colour and withanolides content of Withania somnifera. Withania somnifera (Ashwagandha) is a plant used in medicine from the time of Ayurveda, the ancient system of Indian medicine and belong to the family Solanacae. The dried roots of the plant are used in the treatment of nervous and sexual disorders. From chemistry point of view, the drug contains group of biologically active constituents known as withanolides. Today there is much interest in natural products. The fresh Ashwagandha roots were dried in shade, sun and cabinet dryer. It was found that in cabinet drying about 49 to 52% moisture loss was observed in only 3.15 hours of drying. The drying condition does not affect much on colour but the effect of temperature plays an important role in withanolides content due to which maximum withanolides was observed in shade drying and minimum in case of cabinet drying (air velocity 1.8 - 2.0 ms-1).
Fakultas Farmasi, Universitas Gadjah Mada
2014-06-11 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/4866
Journal of Food and Pharmaceutical Sciences; Vol 2, No 2 (2014): J. Food Pharm. Sci (May-August)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/5369
2014-10-07T02:27:38Z
jfps:ART
2339-0948
2089-7200
dc
Simultaneous Determination Of Metamizole, Thiamin And Pyridoxin In Multicomponent Tablet by RP-HPLC
Chotimah, Chusnul
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
The aim of the study was to develop and validate high performance liquid chromatography (HPLC) assay for the simultaneous determination of Metamizole, Thiamine and Pyridoxin in multicomponent tablet dosage form. The experimental procedure involved reversed-phase-HPLC with a Atlantis T3 C18 column (5 μm particle size, 4.6 ID x 150 mm), PIC solution-metanol-acetic acid volumetric solution (700: 300 : 4, v/v/v) mobile phase, UV detection at 275 nm. The flow rate of the mobile phase was 1.2 mL/min. The method was validated with respect to specificity, precision, accuracy and linearity. Due to its simplicity and accuracy, the assay method is suitable for routine analysis of multiomponen tablet formulation.
Key words : metamizole, thiamine, pyridoxin, HPLC
Fakultas Farmasi, Universitas Gadjah Mada
2014-06-16 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/5369
Journal of Food and Pharmaceutical Sciences; Vol 2, No 3 (2014): J. Food Pharm. Sci (Sept-December)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/5370
2014-10-07T02:27:38Z
jfps:ART
2339-0948
2089-7200
dc
Determination Of Paracetamol, Guaiphenesin, Chlorpheniramine Maleate And Phenylpropanolamine Hydrochloride In Cough And Cold Tablet Using High Performance Liquid Chromatography
Ardiyanti, Yani Abdul
The the National Agency of Drug and Food Control, district of Palangkaraya, Central Kalimantan, Indonesia
The method of choice for analysis of drugs in multi-component preparations is chromatographic based technique such as high performance liquid chromatography (HPLC). A liquid chromatographic method was applied for the analysis paracetamol (PCT), guaiphenesin (GG), chlorpheniramine maleate (CTM) and phenylpropanolamine HCl (PPA) using three different system as described in the USP XXXVI/NF 31. The method depends on a separation on reversed-phase C8, C18 and Phenyl column for analysis PCT, GG, and CTM-PPA respectively with mobile phase water-methanol-acetic acid glacial (79:20:1) for PCT, water-methanol-acetic acid glacial (60:40:1,5) for GG and methanol-water (60:40) containing 0.34 g of monobasic potassium phosphate, 0.05 g of triethylamine hydrochloride, 0.25 g of sodium lauryl sulfate, and 0.1 mL of phosphoric acid in each 100 mL for CTM-PPA. Quantitation was achieved with UV detection at 280, 276 and 214 nm respectively for PCT, GG, and CTM-PPA based on peak area. The methods were validated in terms of system suitability test, specificity, precision, accuracy, linearity, and the results were found to be satisfactory.
Keywords: RP-HPLC, Paracetamol, Guaiphenesin, Chlorpheniramine maleate, Phenylpropanolamine HCl.
Fakultas Farmasi, Universitas Gadjah Mada
2014-06-16 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/5370
Journal of Food and Pharmaceutical Sciences; Vol 2, No 3 (2014): J. Food Pharm. Sci (Sept-December)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/5372
2014-10-07T02:27:38Z
jfps:ART
2339-0948
2089-7200
dc
Influence of Drying on the quality of Ashwagandha (Withania somnifera)
Agrawal, Ravi
Deptt. of Post Harvest Process and Food Engineering, College of Agricultural Engineering, Jawaharlal Nehru Krishi Vishwa Vidyalaya, Jabalpur (M.P.)
An experiment was carried out to adjudge the effect of different drying techniques on colour and withanolides content of Withania somnifera. Withania somnifera (Ashwagandha) is a plant used in medicine from the time of Ayurveda, the ancient system of Indian medicine and belong to the family Solanacae. The dried roots of the plant are used in the treatment of nervous and sexual disorders. From chemistry point of view, the drug contains group of biologically active constituents known as withanolides. Today there is much interest in natural products. The fresh Ashwagandha roots were dried in shade, sun and cabinet dryer. It was found that in cabinet drying about 49 to 52% moisture loss was observed in only 3.15 hours of drying. The drying condition does not affect much on colour but the effect of temperature plays an important role in withanolides content due to which maximum withanolides was observed in shade drying and minimum in case of cabinet drying (air velocity 1.8 - 2.0 ms-1).
Key words: Withania somnifera, withanolides, drying, Ashwagandha.
Fakultas Farmasi, Universitas Gadjah Mada
2014-06-16 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/5372
Journal of Food and Pharmaceutical Sciences; Vol 2, No 3 (2014): J. Food Pharm. Sci (Sept-December)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/5384
2014-10-07T02:27:38Z
jfps:ART
2339-0948
2089-7200
dc
Application of Primer LEP in Detecting Pork Adulteration in Meat Burger Using Hot-Start Real-Time Polymerase Chain Reaction Combined with Melting Curve Analysis
Tjondro, Fortunella
Faculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281, Indonesia.
., Sismindari
Faculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281, Indonesia.
The development of pig species detection in food is increasing due to pork adulteration. Hitherto, the strongest method to detect and quantify pig presence in food is Real-Time Polymerase Chain Reaction. The object of this study is to know whether published primers that amplified leptin gene (LEP primer) could be used to detect and quantify pig’s presence in meat burger using Real-Time Polymerase Chain Reaction with dye intercalator- based detection. Genomic DNA isolation was done by proteinase K digestion. Porcine DNA was amplified using LEP primer with Hot-Start Real-Time Polymerase Chain Reaction combined with Melting Curve Analysis. Condition of Real-Time PCR used in this experiment could amplify not only 152bp porcine leptin gene fragment with Tm value of 83.5oC but also 205bp cow’s leptin gene fragment with Tm 80oC. The result suggests that LEP primer is not a species-specific primer so it can’t be used to quantify pig’s presence in meat burger using Real-Time Polymerase Chain Reaction with dye intercalator-based detection.
Fakultas Farmasi, Universitas Gadjah Mada
2014-06-16 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/5384
Journal of Food and Pharmaceutical Sciences; Vol 2, No 3 (2014): J. Food Pharm. Sci (Sept-December)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/5385
2014-10-07T02:27:38Z
jfps:ART
2339-0948
2089-7200
dc
Studies on Effect of Light Source on the Stability and In Vitro Antioxidant Activity of Dyes and Extracts from Annatto (Bixa orellana L.) Seed
Pamidighantam, Prabhakara Rao
CSIR-Central Food Technological Research Institute (Council of Scientific and Industrial Research), Resource Centre, Habshiguda, Uppal Road, Hyderabad-500 007, India
Isolation of the oleoresin, total lipid, hexane soluble matter (fat), bixin powder and norbixin formulation from annatto (Bixa orellana.L) seed was carried out and the effect of exposure to natural sun light and artificial incandescent light on the stability of bixin, norbixin and their solutions was evaluated. The results could be correlated with data on in-vitro antioxidant activity of the extracts by Ferric reducing power and ABTS assays. Solvent extraction of annatto seed by different methodologies yielded by oleoresin (6.1%), total lipid (5.93%), oil (3.0%) and a natural red dye (1.2%). The stability was higher in dye powders than in solutions when exposed to artificial incandescent light or sunlight. The order of activity as determined by ferric reducing power was, seed oil > oleoresin> total lipid> norbixin> bixin. However, the order of antioxidant activity according to ABTS assay was oleoresin > seed oil > norbixin > total lipid > bixin.
Keywords: bixin, norbixin, annatto extracts, effect of light, antioxidant activity, ferric reducing power, ABTS assay
Fakultas Farmasi, Universitas Gadjah Mada
2014-06-16 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/5385
Journal of Food and Pharmaceutical Sciences; Vol 2, No 3 (2014): J. Food Pharm. Sci (Sept-December)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/5386
2015-12-18T03:44:25Z
jfps:ART
2339-0948
2089-7200
dc
Design and Evaluation of Gastro retentive Drug Delivery System of Anti Ulcer Drug
Patil, J S
Department of Pharmaceutics, SVERI s College of Pharmacy, Gopalpur-Ranjani Road, Gopalpur, Pandharpur-413 304, Maharashtra, India.
Floating matrix tablets of Lansoprezol were developed to prolong gastric residence time, leading to sustained action of the drug. Tablets were prepared by wet granulation technique, using hydroxypropylmethyl-cellulose as a polymer in two different grades, HPMC K4M and HPMC K15M. Tablets were evaluated for their physical characteristics, viz., hardness, thickness, friability, mass variation, drug content and floating properties. Further, tablets were studied for in vitro drug release characteristics for 24 hours. The tablets exhibited controlled and prolonged drug release profiles while floating over the dissolution medium. Non-Fickian diffusion was confirmed as the drug release mechanism from these tablets, indicating that water diffusion and polymer rearrangement played an essential role in drug release. The best formulation (F4) was selected based on in vitro characteristics and was used in vivo radiography studies by incorporating barium sulphate. These studies revealed that the tablets remained in the stomach for 12 hours in fasting rabbits and indicated that gastric retention time was increased by the floating principle, which was considered desirable for the absorption window drugs.
Keywords: Lansoprezol; floating tablets; gastric residence time; gastroretentive drug delivery system
Fakultas Farmasi, Universitas Gadjah Mada
2014-11-20 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/5386
Journal of Food and Pharmaceutical Sciences; Vol 3, No 1 (2015): J. Food Pharm. Sci (January-March)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/5637
2016-09-20T06:06:34Z
jfps:ART
"160609 2016 eng "
2339-0948
2089-7200
dc
Antimicrobial and Volatile Compounds Study of Four Spices Commonly Used in Indonesian Culinary
Hamad, Alwani
University of Muhammadiyah Purwokerto
Mahardika, M. Gigih Panji
University of Muhammadiyah Purwokerto
Istifah, Istifah
University of Muhammadiyah Purwokerto
Hartanti, Dwi
University of Muhammadiyah Purwokerto
The n-hexane extracts of the aerial parts of Ocimum x citriodorum and the leaves of Cymbopogon citratus, Syzygium aromaticum and Syzygium polyanthum were evaluated for their antimicrobial activity against some food borne microorganisms. Their volatile compounds were analyzed by gas chromatography/mass spectrometry (GC/MS). All extracts inhibited the growth of Bacillus subtilis. The extract of S. polyanthum showed the strongest inhibitory activity against Salmonella typhimurium. The growth of Staphylococcus aureus, Escherichia coli and Vibrio cholera were not inhibited by all extracts. The major volatile constituent of O. x citriodorum, C. citratus, S. aromaticum and S. polyanthum crude extracts were citral, germacrene D-14-ol, p-eugenol and squalene, respectively.
Fakultas Farmasi, Universitas Gadjah Mada
2016-06-09 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/5637
Journal of Food and Pharmaceutical Sciences; Vol 4, No 1 (2016): J. Food Pharm. Sci (January-April)
eng
Copyright (c) 2016 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/6081
2016-09-20T06:06:34Z
jfps:ART
"160920 2016 eng "
2339-0948
2089-7200
dc
Evaluation of Microbial Recovery from Raw Materials for Pharmaceutical Use
Eissa, Mostafa
HIKMA Pharma
Mahmoud, Ahmed
Microbiological quality of raw materials used in pharmaceutical manufacturing is critical attribute that affects the bioburden level of the final product. If the preliminary treatment for the microbiological analysis of the material is not suitable to recover actual microbial content, false estimation of microbial presence and/or count in raw materials may impact final product quality and safety. Accordingly a survey study was conducted on 12 selected materials that are used extensively in the manufacturing facility in order find suitable technique to recover low-level inoculums of standard strains. The basic processing and/or neutralization technique was dilution alone or in combination with chemical neutralization or less frequently filtration. Dill oil was the only raw material required combination of dilution and chemical neutralization for both of microbial enumeration and detection while this combination was necessary only in enumeration for Cetostearyl Alcohol. Although dilution method may be adopted alone for Sorbitol solution 70% yet filtration was done in combination to facilitate the visualization of commonly found yeast contaminant from the suppliers of the raw materials. Balsam Peru required the highest dilution among the tested materials for enumeration. The applied neutralization techniques were effective in detecting low level contamination in raw materials.
Fakultas Farmasi, Universitas Gadjah Mada
2016-06-09 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/6081
Journal of Food and Pharmaceutical Sciences; Vol 4, No 1 (2016): J. Food Pharm. Sci (January-April)
eng
Copyright (c) 2016 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/6116
2015-12-18T03:44:25Z
jfps:ART
2339-0948
2089-7200
dc
The use of Fourier Transform Infrared Spectroscopy (FTIR) and Gas Chromatography Mass Spectroscopy (GCMS) for Halal Authentication in Imported Chocolate with Various Variants
Suparman, Suparman
Faculty of Pharmacy, University of Muhammadiyah Purwokerto Jl. Raya Dukuhwaluh P.O.BOX 202, Purwokerto 531187, Indonesia.
The analysis using FTIR and GCMS spectrophotometry for halal authentication on several variants of imported chocolate products circulating on the market has been performed. FTIR spectra analysis result of lard and chocolate in the wave number region of 4000-650 cm-1 shows a typical lard-specific difference at wavenumber region 3006.8 cm-1; 1118.84 cm-1; 1097.42 cm-1. Analysis of PCA and PLS calibration models in the fingerprint region 999.053 - 1190.638cm-1 can be used for lard identification in chocolate fat. The relationship between the actual value and the predicted value of lard in chocolate yields the equation Y= 1,000x-0,0378, (R2 0,997 and RMSEC 1,563) with a minimum limit of detection at a concentration of 4%. Based lard chromatogram, it shows the peak appeared at a retention time of 38,8 minutes. After being compared with library WILLEY7, it shows eikosadienoat 11.14 acidic compounds. Eikosadienoat 11.14 acid is a marker of the presence of lard appearing with a mixture of lard concentration at ≥ 10%. FTIR spectra and analysis results using PCA and PLS on samples of 6 imported chocolate variants show a lard content which is marked by the appearance of eikosadienoat 11.14 acid at a retention time of 38,8 minutes in the chromatogram. PLS quantitative analysis shows lard content in the sample is 43.6%; 73.5%; 61.7%; 63.0%; 37.0%; and 30.4%.
Keywords: FTIR, GCMS, Eikosadienoat, Chocolate
Fakultas Farmasi, Universitas Gadjah Mada
2014-11-20 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/6116
Journal of Food and Pharmaceutical Sciences; Vol 3, No 1 (2015): J. Food Pharm. Sci (January-March)
eng
Copyright (c)
oai:jurnal.ugm.ac.id:article/6163
2015-12-18T03:44:25Z
jfps:ART
2339-0948
2089-7200
dc
Application of chemometrics in combination with Fourier Transform Mid Infrared spectroscopy for authentication of avocado oil
Lumakso, Fajar A.
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Riyanto, Sugeng
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
SA, Shahrel Ahmad
Centre of Research for Fiqh Science and Technology (CFIRST), Universiti Teknologi Malaysia, Skudai, Malaysia.
Rosman, Arieff Salleh
Centre of Research for Fiqh Science and Technology (CFIRST), Universiti Teknologi Malaysia, Skudai, Malaysia.
Yusoff, Farahwahida Mohd.
Centre of Research for Fiqh Science and Technology (CFIRST), Universiti Teknologi Malaysia, Skudai, Malaysia.
Rohman, Abdul
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Centre of Research for Fiqh Science and Technology (CFIRST), Universiti Teknologi Malaysia, Skudai, Malaysia.Research Center of Halal Products, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
This recent study was carried out to develop a rapid, simple, and accurate analytical method for authenticity determination of avocado oil (AO). Fourier transform-mid infrared (FT-MIR) spectroscopy technique aided with Partial Least Square (PLS) were optimized for that purpose to binary mixtures of AO with grapeseed oil (GO) and sesame oil (SeO). The calibration models were constructed at following selected MIR region with normal spectra treatment at combination 1006-902; 1191-1091; and 1755-1654 cm-1 (GO in binary mixture with AO) and 4000-650 cm-1 for analysis of SeO in binary mixture with AO. The high value of coefficient of determination (R2) of 0.9994 with low root mean square error of calibration (RMSEC) value of 0.86 %v/v was revealed in GO quantification. Meanwhile, R2 of 0.9997 with RMSEC 0.73 %v/v were obtained for analysis of SeO in binary with AO. The given value of root mean square error of pprediction (RMSEP) during model validation were 0.52 %v/v (GO) and 0.53 %v/v (SeO), respectively. The high value of R2 and low value of RMSEC and RMSEP during calibration and validation were associated with the accuracy and precision of the used method.
Keywords: avocado oil, FT-MIR spectroscopy, authentication, partial least square.
Fakultas Farmasi, Universitas Gadjah Mada
2014-11-20 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/6163
Journal of Food and Pharmaceutical Sciences; Vol 3, No 1 (2015): J. Food Pharm. Sci (January-March)
eng
Copyright (c) 2015
oai:jurnal.ugm.ac.id:article/6452
2015-12-18T03:44:50Z
jfps:ART
"150501 2015 eng "
2339-0948
2089-7200
dc
Nutritional Composition of Momordica dioica fruits: As a wild vegetable
Salvi, Jyotsna
Department of Botany,University College of Science, Mohan Lal Sukhadia University, Udaipur, Rajasthan, India
Samples of Momordica dioica Roxb. ex Willd fruits were collected from Jhadol Tehsil of southern Rajasthan. The fruits were washed, ground into powder and analyzed for the proximate, vitamins, mineral and Fatty acid compositions. The values (% DW) of moisture, ash, crude protein, crude fiber, crude fat and carbohydrate available were 87, 14, 52.06, 15.36, 4 and 14.58, respectively. Levels (g/100g DW) of vitamins A, B1, B2, B6, H and K were 2.5, 1.8, 3.5, 4.3, 6.5 and 15 respectively. The values (mg/100g DW) of minerals potassium, sodium, calcium, zinc, Copper and magnesium were 370, 58, 26000, 8.5, 1.7 and 14000 respectively. Comparing the mineral and vitamin composition with recommended dietary allowances (RDA), the result indicated that Momordica dioica fruits could be good supplement for vitamin B1, B2, B6, B9, Ca and Mg. The values (% DW) of fatty acids i.e. Oleic acid, Palmitic acid, Linoleic acid, Myristic acid and Stearic acid of fruits were 56.253, 12.157, 22.511, 3.589 and 3.547 respectively. The results indicated that Momordica dioica fruits could be a good supplement for some nutrients, minerals and fatty acids such as fiber, protein, carbohydrates, calcium, magnesium, Oleic acid, and Linoleic acid. The fruit could be promoted as a mineral and vitamin supplement for cereal-based diets in poor rural communities.
Fakultas Farmasi, Universitas Gadjah Mada
2015-10-21 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/6452
Journal of Food and Pharmaceutical Sciences; Vol 3, No 2 (2015): J. Food Pharm. Sci (April-June)
eng
Copyright (c) 2015 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/6508
2016-03-29T07:01:53Z
jfps:ART
"160329 2016 eng "
2339-0948
2089-7200
dc
DETERMINATION OF NUTRIENT COMPOSITIONS, GLYCEAMIC INDEX AND ANT-DIABETIC POTENTIALS OF MULTI-PLANT BASED FUNCTIONAL FOODS IN RATS
Steve, Ijarotimi Oluwole
Department of Food Science and Technology, Federal University of Technology, Akure, Nigeria http://www.futa.edu.ng
Background: Nutrition transition, that is, increase in consumption of high energy-dense foods, with low consumption of fruits and vegetables, has been implicated as the major factors responsible for the increase in prevalence of diet-related diseases such as diabetes and cardiovascular diseases worldwide. Evidence has shown that consumption of plant-based foods prevents the risk of these chronic diseases, hence, the present study formulated and evaluated nutrient compositions and antidiabetic potentials of multi-plant based functional foods from locally available food materials.
Methods: Food materials (popcorn, moringa leaves, wonderful kola and defatted soybean) were obtained from reputable farms and markets in Akure, Nigeria. The food materials were processed as raw, blanched and fermented flour samples, and blended to obtain nine samples, i.e., R1, B1 and F1 (popcorn 60%, soybean 10%, moringa 20% and groundnut oil 10%), R2, B2 and F2 (popcorn 60%, soybean 10%, wonderful kola 20% and groundnut oil 10%) and R3, B3 and F3 (popcorn 60%, soybean 10%, moringa 10%, wonderful kola 10% and groundnut oil 10%) using Nutri-Survey software. Proximate compositions of the blended samples were determined using AOAC methods, and glyceamic index and anti-diabetic potentials were determined using rat models.
Results: Proximate compositions (g/100g) of the formulated multi-plant based functional foods were as follows: moisture contents ranged from 6.29 in F3 to 8.27 in R3, crude fiber contents from 2.79 in F2 to 4.68 in B3 and crude protein contents from 23.22 in B1 to 30.39 in F3, while carbohydrate content of the formulations ranged from 52.10 in F3 sample to 56.94 in B2 sample, while energy values were between 421.1 in R1 and 433.7 kcal in B1. Glycaemic index (GI) of the formulated functional foods ranged from 5.8% in R3 to 28.9% in R1 sample, and were significantly (<0.05) lower than in glucose (a reference sample). The glycaemic load (GL) of R4 sample had the lowest value (3.3), while F4 sample had the highest value (15.1). The percentage blood glucose reduction of diabetic-induced rats fed with R1samples (63.8%) had the highest values; while those rats fed with B2 sample (24.1%) had the lowest blood glucose reduction. Statistically, the percentage blood glucose reduction of the formulated functional foods, particularly R1 and F1, were comparable to metformin (a synthetic anti-diabetic drug) in terms of antidiabetic activities.
Conclusion: The study reported on the nutritional profile of multi-plant based functional foods from popcorn, soybean, wonderful kola and moringa leaves. Findings showed that these functional foods contain appreciable amount of protein, fiber, carbohydrate content within the recommended value for diabetic patients, low glycaemic index and glycaemic load properties and with antidiabetic activities. Hence, the formulated functional foods may be suitable for individuals at risk of diabetes or diabetic patients.
Fakultas Farmasi, Universitas Gadjah Mada
2016-03-29 14:01:55
application/pdf
https://journal.ugm.ac.id/jfps/article/view/6508
Journal of Food and Pharmaceutical Sciences; Vol 3, No 3 (2015): J. Food Pharm. Sci (September-December)
eng
Copyright (c) 2016 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/7391
2015-12-18T03:44:50Z
jfps:ART
"151218 2015 eng "
2339-0948
2089-7200
dc
Physico-chemical Amino acid composition, fatty acid profile, functional and antioxidant properties of Spinacia oleracea L. leaf
Pamidighantam, Prabhakara Rao
CSIR Central Food Technological Research Institute, Resource Centre, Hyderabad, Telangana, India
Galla, Narsing Rao
CSIR CFTRI Resource Centre, Hyderabad
Galla, Narsing Rao
CSIR CFTRI Resource Centre, Hyderabad
Guruguntla, Sulochanamma
CSIR CTRI Resource Centre, Hyderabad
Guruguntla, Sulochanamma
CSIR CTRI Resource Centre, Hyderabad
Akula, Satyanarayana
CSIR CFTRI Resource centre, Hyderabad
Akula, Satyanarayana
CSIR CFTRI Resource centre, Hyderabad
Spinach (Spinacia oleracea L.) leaf powder (SLP) was prepared and its physicochemical (proximate composition, colour, mineral content, EMC-RH, protein solubility, amino acid content and fatty acid profile), functional (water absorption, fat absorption, emulsification and foam capacities) and antioxidant (DPPH, ABTS and Iron reducing power) properties were studied. SLP possessed a protein content of 31.72%, calcium (1335 mg/100g), iron (29 mg/100g) and phosphorous (278 mg/100g). Moisture sorption isotherms of SLP indicated its non-hygroscopic nature. Solubility of SLP protein in distilled water was 39.66%, which got enhanced to 72.83% in 0.1M NaCl at pH 12. The spinach leaf lipid was found to be rich in ω-3 fatty acids (67.4%). Leucine (11.87%), phenylalanine (5.9%) and valine (9.24%) were the major essential amino acids observed in SLP. Functional properties of SLP exhibited good water absorption, fat absorption and foam capacities. Antioxidant activity of SLP determined by the 2,2-azino-bis (3-ethyl benzothiazoline 6- sulphonic acid (ABTS) assay was found higher, when compared to the activities determined by diphenyl picrylhydrazyl (DPPH) and ferric reducing power assays. Protein solubility, amino acid content, fatty acid profile, functional properties and antioxidant characteristics encouraging for food and pharmaceutical applications.
Fakultas Farmasi, Universitas Gadjah Mada
2015-10-21 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/7391
Journal of Food and Pharmaceutical Sciences; Vol 3, No 2 (2015): J. Food Pharm. Sci (April-June)
eng
https://journal.ugm.ac.id/jfps/article/download/7391/13047
Copyright (c) 2015 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/7507
2015-12-18T03:44:50Z
jfps:ART
"151218 2015 eng "
2339-0948
2089-7200
dc
“Effect of various drying methods on quality and flavor characteristics of mint leaves (Mentha spicata L.)”
Kripanand, Sathiyamala
Guruguntla, Sulochanamma
korra, srinivasulu
CSIR-Central food Technological Research Institute
Mint leaves were dehydrated by hot air, shade as well as microwave drying and the respective drying time found, to lower the moisture content from (88%) to around (5%). The qualities of the dried products were assessed by determining the moisture, chlorophyll, carotenoid, polyphenols, color and volatile oil. In microwave drying, despite the less drying time, there were appreciable losses of volatile oil, chlorophyll and other components when compared to the fresh mint leaves. Results showed that Hot Air Drying (HAD) at 45°C followed by Micro Wave Drying (MWD) at 900 Watts possessed better quality parameters in the dried products suggesting that Hot Air Drying (HAD) was most suitable although it took more time compared to Micro Wave Drying (MWD).
Fakultas Farmasi, Universitas Gadjah Mada
2015-10-21 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/7507
Journal of Food and Pharmaceutical Sciences; Vol 3, No 2 (2015): J. Food Pharm. Sci (April-June)
eng
Copyright (c) 2015 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/7607
2015-12-18T03:44:50Z
jfps:ART
"151218 2015 eng "
2339-0948
2089-7200
dc
Development and validation of atomic absorption spectrometry for the determination of zink and mercury analyzer for determination of Mercury in cream cosmetics
Rohman, Abdul
Wijayanti, Erni
2 Faculty of Pharmacy, Gadjah Mada University, Yogyakarta 55281, Indonesia
An atomic absorption spectrometry for the quantification of zink (Zn) and mercury analyzer for the quantification of mercury (Hg) levels in cream cosmetic were developed and validated. The method was validated for linearity and range, precision, accuracy, limit of detection (LOD) and limit of quantification (LOQ). The standard curves were linear over the concentration of 0.1-1.2 µg/mL (Zn) and 0.05-2.0 µg/L (Hg) with coefficient of correlation (r) > 0.99. The detection limits obtained were 2.8614 µg/g sample (Zn) and 0.4749 ng/g sample. The quantification limit obtained were 9.5281 µg/g sample (Zn) and 1.5827 ng/g sample. The relative standar deviation (RSD) values found to be 8.67 % for its intraday precision and 9.89 % for its interday precision (Zn) and 12.69 % for its intraday precision and 7.17 % for its interday precision (Hg). These RSD values are lower than those required by RSD Horwitz unction. The mean recovery percentage was 94.28 % (for Zn) and 78.65% (for Hg). These developed methods were succesfully used for determination of Zn and Hg in cream cosmetics products.
Key words: mercury, zink, atomic absorption spectroscopy, mercury analyzer, cream cosmetic
Fakultas Farmasi, Universitas Gadjah Mada
2015-10-21 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/7607
Journal of Food and Pharmaceutical Sciences; Vol 3, No 2 (2015): J. Food Pharm. Sci (April-June)
eng
Copyright (c) 2015 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/8774
2016-03-29T07:01:53Z
jfps:ART
"160329 2016 eng "
2339-0948
2089-7200
dc
Tiron Mitigates Thioacetamide-Induced Acute Liver Injury
Shoeib, Amal Mahmoud
Faculty of Pharmacy, Mansoura University
Said, Eman
Pharmacology and Toxicology dep., Faculty of Pharmacy, Mansoura University
Nader, Manar Ahmed
Pharmacology and Toxicology dep., Faculty of Pharmacy, Mansoura University
Salem, Hatem Abd-Elrahman
Pharmacology and Toxicology dep., Faculty of Pharmacy, Mansoura University
Ammar, Elsayed Mohamed
Pharmacology and Toxicology dep., Faculty of Pharmacy, Mansoura University
Acute liver injury is a crippling disorder accompanied by extensive impairment of liver’s synthetic, metabolic and detoxifying functions. Tiron is a synthetic vitamin E analog, proven to be anti-oxidant. This study was undertaken to investigate the protective activity of tiron against thioacetamide (TAA)-induced acute liver injury. Rats were orally treated with tiron (300 mg/kg) for eight days prior to I.V. TAA either (70 mg/kg) or (280 mg/kg) to induce acute liver injury. Biochemical evaluation of hepatotoxicity indices, oxidative stress, cytotoxicity and inflammatory marker: interleukin-6 (IL-6) was conducted along with histopathological examination. Meanwhile, tiron was found to mitigate the TAA-induced elevation of ALT, AST and ALP. However, serum albumin levels mildly improved. Tiron restored liver GSH contents and maintained liver SOD activity. Moreover, tiron significantly reduced the level of serum IL-6. In context, histopathological examination revealed that tiron slightly reduced the extent of TAA-induced necrosis. Tiron has manifested the observed hepatoprotective effect probably by manipulating inflammatory response of liver to injury via downregulating the expression of inflammatory IL-6 and alleviating oxidative stress.
Fakultas Farmasi, Universitas Gadjah Mada
2016-03-29 14:01:55
application/pdf
https://journal.ugm.ac.id/jfps/article/view/8774
Journal of Food and Pharmaceutical Sciences; Vol 3, No 3 (2015): J. Food Pharm. Sci (September-December)
eng
Copyright (c) 2016 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/8916
2016-03-29T07:01:52Z
jfps:ART
"160329 2016 eng "
2339-0948
2089-7200
dc
ELECTRICAL STUNNING AND PRE-SLAUGHTERING TIME INTERVAL EFFECT IN EXPRESSION OF HEART BROILER CHICKENS PROTEIN
Hermanto, Sandra
Syarif Hidayatullah State Islamic University
Pre-slaughtering process by transporting broiler chickens from farm to slaughterhouse and treatment of electrical stunning could be one of halal critical point. Stressing on chickens could be trigger a specific protein expression or increase its expression as a result of changes in metabolism that occurs. The purpose of this study was to identify the effect of treatment of electrical stunning and the pre-slaughtering interval time on broiler chicken liver protein expression through the analysis of protein profile by SDS-PAGE. Experiments conducted on 7 broiler chickens aged 35 days were given pre-slaughter treatment are different. Treatments include electrical stunning of 0 V, 25 V and 250 V as well as the pre-slaughtering time interval 0 hours, 2 hours, 2 hours + 12 hours (fasting) and 2 hours + 12 (fasting) with electrical stunning 250 V. Each of samples (chicken liver protein) isolated by homogenized in Trs-HCl buffer and the protein concentration determined by Lowry method. Identification of the protein profile was done by observing the differences in the intensity of the electrophoresis results followed by densitometry analysis. The results showed significant differences in outcomes electrical stunning treatment as indicated by the appearance of protein bands at around 109 kDa and do not appear on other treatments. The difference is predicted from particular protein expressed induced by cell metabolism responsive in chicken liver cell.
Fakultas Farmasi, Universitas Gadjah Mada
2016-03-29 14:01:55
application/pdf
https://journal.ugm.ac.id/jfps/article/view/8916
Journal of Food and Pharmaceutical Sciences; Vol 3, No 3 (2015): J. Food Pharm. Sci (September-December)
eng
Copyright (c) 2016 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/12184
2016-09-20T06:19:39Z
jfps:ART
"160920 2016 eng "
2339-0948
2089-7200
dc
Optimization of Self-nanoemulsifying Drug Delivery System for Pterostilbene
Puspita, Oktavia Eka
Universitas Brawijaya
Suwaldi, Suwaldi
Universitas Gadjah Mada
Nugroho, Akhmad Kharis
Universitas Gadjah Mada
Solubility is prerequisite for drug absorption across absorptive cell lining the small intestine. It is a problem for poor water soluble drug because limiting its bioavailability when administered by oral route. Lipid based delivery system such as self-nanoemulsifying delivery system (SNEDDS) can be utilized in improving its solubility so that better bioavailability is achieved. Pterostilbene has extremely low solubility in water then become its limiting factor for the bioavailability. This research developed SNEDDS for oral delivery of pterostilbene. Optimum composition of SNEDDS formulation was judged by its dispersion efficiency and clarity when dispersed in water. The efficiency of this formula in enhancing bioavailability was assessed by in vitro digestion model to predict its bioavailability by determining its bioaccessibility. The result showed that optimum composition of SNEDDS was achieved by soybean oil-Croduret® 50-Span 80-PEG 400 in ratio of 16.37 %, 32.07 %, 11.56 %, and 40 %, respectively. This formula has bioacessibility of 91.48 ± 2.18 %, and it is much higher compared to pterostilbene that was not formulated into SNEDDS, i.e 4.63 ± 1.11 %. Determined by dynamic light scattering, this optimum formula has droplet size of 31.8 nm when dispersed in water.
Fakultas Farmasi, Universitas Gadjah Mada
2016-09-20 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/12184
Journal of Food and Pharmaceutical Sciences; Vol 4, No 2 (2016): J. Food Pharm. Sci (May-August)
eng
Copyright (c) 2016 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/12872
2016-11-28T01:36:15Z
jfps:ART
"160920 2016 eng "
2339-0948
2089-7200
dc
Antioxidant Activity Test Of Total Flavonoids Mistletoe Leaves (Dendrophthoe pentandra (L.) Miq) From False Ashoke Tree
Fahmi, Aliyah
USU http://orcid.org/0000-0002-1825-0097 http://orcid.org/0000-0002-1825-0097
NST, Rumondang Bulan
Chemistry of Math & Science Faculty USU, North Sumatera
N, Hamonangan
Chemistry of Math & Science Faculty USU, North Sumatera
Research on the antioxidant activity test of total flavonoids mistletoe leaves (Dendrophthoe pentandra (L.) Miq) from false ashoke tree (Polyalthia longifolia) had been done. This study aimed to determine antioxidant activity of total flavonoids mistletoe leaves based on reduction method of free radicals 2.2- diphenyl-1-picrylhydrazyl (DPPH) 200 ppm using a UV Visible Spectrophotometer at maximum wavelength 516 nm was obtained Inhibition Concentration (IC50) amount 6,16 mg / L with the reduction percentage of 98.61% at 100 ppm which means it had a very strong antioxidant activity.
Keywords: mistletoe leaves, Dendrophthoe pentandra (L.) Miq, antioxidant activity ,false ashoke tree
Fakultas Farmasi, Universitas Gadjah Mada
2016-09-20 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/12872
Journal of Food and Pharmaceutical Sciences; Vol 4, No 2 (2016): J. Food Pharm. Sci (May-August)
eng
https://journal.ugm.ac.id/jfps/article/download/12872/25756
https://journal.ugm.ac.id/jfps/article/download/12872/25762
Copyright (c) 2016 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/13045
2016-09-20T06:19:39Z
jfps:ART
"160920 2016 eng "
2339-0948
2089-7200
dc
Determination of Rifampicin, Isoniazid, Pyrazinamide and Ethambutol Hydrochloride in 4FDC Tablet by FTIR Spectrophotometry in Combination with Multivariate Calibration
Kurniati, Zakiah
National Agency of Drug and Food Control, district of Medan, Indonesia
Departement of Pharmaceutical Chemistry, Faculty of Pharmacy Gadjah Mada University, Yogyakarta 55281, Indonesia
Riyanto, Sugeng
Departement of Pharmaceutical Chemistry, Faculty of Pharmacy Gadjah Mada University, Yogyakarta 55281, Indonesia
Rohman, Abdul Abdul
Departement of Pharmaceutical Chemistry, Faculty of Pharmacy Gadjah Mada University, Yogyakarta 55281, Indonesia
Research Centre of Halal Products, Gadjah Mada University Yogyakarta 55281, Indonesia.
This study was aimed to develop a fast, inexpensive, simple, and not involving hazardous reagent for the determination of rifampicin (RIF), isoniazid (INH), pyrazinamide (PZA) dan ethambutol hydrochloride (ETH) in 4FDC tablet based on infrared spectrophotometry in combination with chemometrics of partial least square (PLS) calibration. The analytical procedure involves three steps, namely calibration, validation and sample assay. The multivariate calibration model is evaluated by determining the precision and accuracy parameters, as suggested by IUPAC. The results showed that the optimized wavenumber range for the determination of RIF was the combined wavenumbers of 433 – 1552 cm-1 and 1756 – 3412 cm-1, the wavenumber of 433 – 873 cm-1 was selected for quantification of INH, 1714 – 2756 cm-1 for PZA, and 1552 – 2970 cm-1 for ETH. The predicted residual error sum of square (PRESS) values of RIF, INH, PZA and ETH contents were 0.00392%, 0.011724%, 0.007604%, and 0,003368%, respectively. The root mean square error of prediction (RMSEP) values obtained for analysis of RIF, INH, PZA and ETH were 0.01979%, 0.03424%, 0.027575% and 0.018351%, respectively. The values of coefficient of determination (R2) for the relationship between actual values of these drugs and calculated values of these drugs using FTIR spectroscopy were 0.990, 0.977, 0.992 and 0.998 for RIF, INH, PZA and ETH, respectivelty. The developed method was succesfully applied for the determination of 4FDC tablet. The contents of RIF, INH, PZA and ETB in 4FDC tablets were in agreement with those specified in monograph of Indonesian Pharmacopeia.
Keywords: infrared spectrophotometry, rifampicin, isoniazid, pyrazinamide, ethambutol hydrochloride, partial least square.
Fakultas Farmasi, Universitas Gadjah Mada
2016-09-20 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/13045
Journal of Food and Pharmaceutical Sciences; Vol 4, No 2 (2016): J. Food Pharm. Sci (May-August)
eng
Copyright (c) 2016 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/17246
2017-10-17T07:28:49Z
jfps:ART
"170103 2017 eng "
2339-0948
2089-7200
dc
OPTIMATION OF COMPOSTION OF SPAN 80-CRODURET 50-PROPYLENE GLYCOL AS EMULSIFIER OF STRAWBERRY JUICE (Fragaria vesca L.) EMULGEL AND TRANSPORT TEST THROUGH SHED SNAKE SKIN MEMBRANE
ERMAWATI, DIAN EKA
DEPARTEMEN OF PHARMACEUTICS, FACULTY OF PHARMACY, GADJAH MADA UNIVERSITY, YOGYAKARTA http://dianekaerma http://orcid.org/0000-0002-8633-9261
Strawberry (Fragaria vesca L.) contains many nutrients that are beneficial to health because rich of antioxidant such as ascorbic acid, quercetin-3-β-D–glucoside, anthocyanin pelargonidin-3-O-glucoside and cyanidin-3-glucoside. Amount of anthocyanins and flavonoids in strawberry during process will decline respectively 53% for pelargonidin-3-O-glucoside and 40% for flavonoid quercetin. Using water-oil emulsion (w/o) topically less convenient, so it needs to be dispersed in the matrix gel to help sustain the permeation of the active components of strawberry juice into the stratum corneum. Emulsion consisting of water and oil components are not mixed with each other, thus requiring additional emulsifier to create a system that is homogeneous and stable.
The proportion of combination of emulsifier as span 80, croduret 50, propylene glycol are optimized, to produce an optimum formula with good physical stability. The optimization method was Simplex Lattice Design by using Design Expert® software 7.1.5. The results of the response of the assessed physical parameter were viscosity, separation ratio (F), and diameter of emulsion globule. The optimum formula have measured physical stability test, active ingredient of flavonoid and anthocyanins, which then was conducted transport test through the membrane of shed snake skin by Franz’s model diffusion cells with the flavonoid concentration parameters that were able to pass through the membrane.
Emulsion w/o that use combination of 2,00% Span 80; 4,00% croduret 50, and 2,00% of propylene glycol which produce a physically stable emulsion base on Simplex Lattice Design [p-value> 0.05]. Strawberry juice contains 23,3 % of flavonoid, 13,23% of anthocyanin, and IC50 331 µg/mL. The optimum formula of emulgel contains 20% of flavonoids and 11.67% of anthocyanin. The cumulative amount of flavonoid which was transported through the shed snake skin membrane was 117,14 µg/cm2 during five hours. The permeability of membrane shed snake skins is 2,84x10-5 µg/cm2and flux value is 6,6x10-5 µg/secon.
Keywords : strawberry juice, emulsifier, Simplex Lattice Design, emulgel, flavonoid
Fakultas Farmasi, Universitas Gadjah Mada
2016-11-28 00:00:00
https://journal.ugm.ac.id/jfps/article/view/17246
Journal of Food and Pharmaceutical Sciences; Vol 4, No 3 (2016): J. Food Pharm. Sci (September-December)
eng
https://journal.ugm.ac.id/jfps/article/download/17246/33759
Copyright (c) 2017 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/27326
2016-09-20T06:06:34Z
jfps:ART
"160812 2016 eng "
2339-0948
2089-7200
dc
Toxicity Test Pediocin N6 Powder Produced from Isolates Pediococcus Pentosaceus Strain N6 on White Mice
Ketaren, Nurjama'yah Br.
Department of Agriculture, Faculty of Agriculture, Al- Azhar University Medan, Medan 20142, Indonesia
Marlida, Yetti
Department of Agriculture, Faculty of Animal Husbandry, Andalas University, Padang 25163, Indonesia
Arnim, Arnim
Department of Agriculture, Faculty of Animal Husbandry, Andalas University, Padang 25163, Indonesia
Yuherman, Yuherman
Department of Agriculture, Faculty of Animal Husbandry, Andalas University, Padang 25163, Indonesia
Rusmarilin, Herla
Department of Agriculture Technology, Faculty of Agricultural, University of North Sumatra, Medan 20155, Indonesia
Pediocin N6 powder is a bacteriocin the heat resistant derived from isolates Pediococcus pentosaceus strain N6. These were isolated from water source heat Rimbo Panti West Sumatra. Pediocin N6 powder has high antimicrobial activity, so the potential to be used as biopreservatif on meat and food processing industry which involves heating. Toxicity test was conducted to determine the effects of the toxic effect of a single dose of oral Pediocin N6 powder in test animals male white mice to determine the LD50 and see changes in body weight of mice for 15 days of treatment. Changes in body weight of mice was determined by using non factorial experiment in a completely randomized design consists of 4 treatments and 5 replications. The test animals were divided into 4 groups and each treatment consisted of 5 mice. The treatments tested consisted of Pediocin N6 powder 5000 mg/kg body weight, 10,000 mg/kg body weight, 15,000 mg /kg and 20,000 mg /kg body weight of mice. The test results showed that the Pediocin N6 powder up to a dose of 20 000 mg/kg in a single oral dose administration, there are no death of mice to 15 days of treatment. Based on the LD50 value of a single oral dose can not be calculated, based on it can be stated LD50 value pseudo Pediocin N6 powder greater than 20,000 mg /kg in male mice. The average changes in body weight of mice at a dose of Pediocin N6 powder treatment of up to 20,000 mg/kg every 2 days weighing from day 1 to day 15 of 2.1 gr. Based on these tests Pediocin N6 powder safe used as industry biopreservatif on meat and food processing involves heating.
Key word: Toxicity Test, Pediocin N6 Powder, White Mice, Biopreservatif on Meat
Fakultas Farmasi, Universitas Gadjah Mada
2016-06-09 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/27326
Journal of Food and Pharmaceutical Sciences; Vol 4, No 1 (2016): J. Food Pharm. Sci (January-April)
eng
Copyright (c) 2017 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/27336
2017-10-17T07:28:49Z
jfps:ART
"170708 2017 eng "
2339-0948
2089-7200
dc
Optimum Dose and Formulation of Centella asiatica L. Urban Extract Against IgG of Wistar Strain Male Mices which Induced by BCG Vaccine
Ermawati, Dian Eka
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
Sasmito, Ediati
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
Mufrod, Mufrod
Department of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
ND., Pramitha Esha
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
UA., Ni Putu
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
KD, Anggi
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
H., Muchammad
Department of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
S., Aini
Department of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
Objective: Centella asiatica L.Urban contains triterphenoid saphonin, flavonoid, and pectin that believed to have an immunomodulatory effect toward the Immunoglobulin-G in Wistar strain male mices which Induced by BCG vaccine. A study was conducted to find out the optimum dose and formulation of in enhancing on the level of IgG. It’s therefore expected that Cantella asiatica L.Urban can be used as an immunomodulatory suplement in both animals and human.
Methods: Centella asiatica L.Urban were macerated with ethanol 50%. This was an experimental study with the post-test control group design. The samples in this study were 35 Wistar strain male mices which divided into 7 groups: negative control [Aquadest 0,5mL/20g BW]; control immunosupresant [Prednison® 0,06mg/20g BW]; control immunostimulant [Levamisol® 0,445mg/20g BW]; ethanolic extract of Cantella asiatica L.Urban with a dose of 50mg; 100mg; 150mg; 200mg/Kg B.W were administered orally fo 10 day, after the treatments implemented, BCG [Bacillus Calmette Guerin] vaccine was infected intraperitoneally on day 10th, 14th, 21st, and 28th. The most effective doses of ethanolic extract of Cantella asiatica L.Urban was further studied for its effect on hormonal assay using ELISA reader which compared with the effects of Prednison® and Levamisol®. Based on simplex lattice design to find the optimum proportion of coefficients a, b, and ab of the equation Y = a (A) + b (B) + ab (A) (B), where A is lactose and B is amylum.
Results : Oral administration of ethanolic extract of Cantella asiatica L.Urban at dose of 50mg and 100mg/Kg B.W increased the immunity which showed elevated levels of IgG in the blood serum in Wistar strain male mices that had been induced by BCG vaccine. The optimum formula capsul of Centella asiatica L.Urban extract that use combination of 80% Amylum and 20% Lactose which produce the optimum parameters of disintegration time (≤15minutes) and CV of weight uniformity (≤5%)with the test results had no significant difference to the prediction results of Simplex Lattice Design [p>0.05].
Keywords: Centella asiatica L.Urban extract, Immunomodulator, simplex lattice design
Fakultas Farmasi, Universitas Gadjah Mada
2016-11-28 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/27336
Journal of Food and Pharmaceutical Sciences; Vol 4, No 3 (2016): J. Food Pharm. Sci (September-December)
eng
Copyright (c) 2017 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/27337
2017-10-17T07:28:49Z
jfps:ART
"170327 2017 eng "
2339-0948
2089-7200
dc
Optimum Dose and Formulation of Centella asiatica L. Urban Extract Against IgG of Wistar Strain Male Mices which Induced by BCG Vaccine
Ermawati, Dian Eka
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
Sasmito, Ediati
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
Mufrod, Mufrod
Department of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
Esha ND., Pramitha
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
Putu UA., Ni
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
KD, Anggi
Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
Muchammad H., Muchammad
Department of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
Aini S., Aini
Department of Pharmaceutics, Faculty of Pharmacy, Gadjah Mada University, Yogyakarta
Objective: Centella asiatica L.Urban contains triterphenoid saphonin, flavonoid, and pectin that believed to have an immunomodulatory effect toward the Immunoglobulin-G in Wistar strain male mices which Induced by BCG vaccine. A study was conducted to find out the optimum dose and formulation of in enhancing on the level of IgG. It’s therefore expected that Cantella asiatica L.Urban can be used as an immunomodulatory suplement in both animals and human.
Methods: Centella asiatica L.Urban were macerated with ethanol 50%. This was an experimental study with the post-test control group design. The samples in this study were 35 Wistar strain male mices which divided into 7 groups: negative control [Aquadest 0,5mL/20g BW]; control immunosupresant [Prednison® 0,06mg/20g BW]; control immunostimulant [Levamisol® 0,445mg/20g BW]; ethanolic extract of Cantella asiatica L.Urban with a dose of 50mg; 100mg; 150mg; 200mg/Kg B.W were administered orally fo 10 day, after the treatments implemented, BCG [Bacillus Calmette Guerin] vaccine was infected intraperitoneally on day 10th, 14th, 21st, and 28th. The most effective doses of ethanolic extract of Cantella asiatica L.Urban was further studied for its effect on hormonal assay using ELISA reader which compared with the effects of Prednison® and Levamisol®. Based on simplex lattice design to find the optimum proportion of coefficients a, b, and ab of the equation Y = a (A) + b (B) + ab (A) (B), where A is lactose and B is amylum.
Results : Oral administration of ethanolic extract of Cantella asiatica L.Urban at dose of 50mg and 100mg/Kg B.W increased the immunity which showed elevated levels of IgG in the blood serum in Wistar strain male mices that had been induced by BCG vaccine. The optimum formula capsul of Centella asiatica L.Urban extract that use combination of 80% Amylum and 20% Lactose which produce the optimum parameters of disintegration time (≤15minutes) and CV of weight uniformity (≤5%)with the test results had no significant difference to the prediction results of Simplex Lattice Design [p>0.05].
Keywords: Centella asiatica L.Urban extract, Immunomodulator, simplex lattice design
Fakultas Farmasi, Universitas Gadjah Mada
2016-11-28 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/27337
Journal of Food and Pharmaceutical Sciences; Vol 4, No 3 (2016): J. Food Pharm. Sci (September-December)
eng
Copyright (c) 2017 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/28161
2017-09-08T07:30:12Z
jfps:ART
"170908 2017 eng "
2339-0948
2089-7200
dc
Physico-chemical Properties, biological activities and authentication of cod liver oil
Rohman, Abdul
Department of Pharmaceutical chemistry, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Research Center of Halal Products, Universitas Gadjah Mada, Yogyakarta 55281, Indonesia
Cod liver oil (CLO), currently, has attracted public awareness and researchers as functional food oils. It is predicted that CLO will experience a great market in the fats and oils industry. CLO has been known to have several biological activities and its price is much higher (10–15 times) than common edible oils, thus CLO is subjected to adulteration with lower priced oils, therefore its authentication is highlighted in this review. This article highlighted some of the reported activities of CLO like the prevention of coronary heart disease. In addition, physico-chemical properties of CLO were also described in this review. Some analytical methods, especially spectroscopy and chromatographic based techniques along with its variation have been used for authentication of CLO from oil adulterants. FTIR spectroscopy along with multivariate calibrations have been reported to be used for authentication of CLO from animal fats and vegetable oils fruitfully. Because of its capability to separate specific components in CLO, chromatographic techniques are suitable for such authentication using specific markers.
Keywords: cod liver oil, physico-chemical properties, biological activity, authentication.
Fakultas Farmasi, Universitas Gadjah Mada
2017-09-08 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/28161
Journal of Food and Pharmaceutical Sciences; Vol 5, No 1 (2017): J. Food Pharm. Sci (January-April)
eng
Copyright (c) 2017 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/28162
2017-09-08T08:55:13Z
jfps:ART
"170220 2017 eng "
2339-0948
2089-7200
dc
Authentication of Wild Boar Meat in Meatball Formulation Using Differential Scanning Calorimetry and Chemometrics
Guntarti, Any
Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281, Indonesia
Faculty of Pharmacy, Ahmad Dahlan University, Yogyakarta, 55166, Indonesia
Rohman, Abdul
Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281, Indonesia
Research Center of Halal Products, Gadjah Mada University, Yogyakarta, 55281, Indonesia
Martono, Sudibyo
Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281, Indonesia
Yuswanto, Agustinus
Faculty of Pharmacy, Gadjah Mada University, Yogyakarta, 55281, Indonesia
Bakso or meatball is one of the Indonesian favorite foods, commonly made from beef. This food is quite popular among Indonesian societies. Due to the high price of beef, unethical producers may adulterate beef with wild boar meat (WBM). In this study, the potential use of differential scanning calorimetry (DSC) combined with multivariate calibration was used to verify adulteration of WBM in meatball formulation. Oil extracted from WBM is characterized by significantly different cooling and heating DSC thermal profiles. The change of characteristic exothermic and endothermic event in oil with increasing crystallization, melting enthalpy and developing both process over a narrower temperature range is investigated. In this research, we developed DSC and multivariate calibration of Partial Least Square (PLS) calibration to analyze WBM in beef meatball. Meanwhile, the chemometrics of Principle Componen Analysis (PCA) is used to classify WBM and beef in the meatball. The validation model using crystalization profiles yield the coefficient of determination (R2) of 0.999 for the correlation between actual value of WBM (x-axis) and DSC predicted value (y-axis) with equation of y= 0.9999 x + 0.0027, root mean square error of cross validation (RMSECV) of 0.380%, and root mean square error of prediction (RMSEP) of 0.203%. PCA is successfully used for classification of WBM in beef meatball. DSC in combination with PLS and PCA can be an alternative technique for analysis of WBM in meatball.
Key words: Differential scanning calorimetry, Wild bear meat, Crystallization profile, Melting profile, Partial least square.
Fakultas Farmasi, Universitas Gadjah Mada
2017-09-08 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/28162
Journal of Food and Pharmaceutical Sciences; Vol 5, No 1 (2017): J. Food Pharm. Sci (January-April)
eng
Copyright (c) 2017 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/30449
2017-11-21T03:19:20Z
jfps:ART
"170822 2017 eng "
2339-0948
2089-7200
dc
Progress in Synthesis of Monoglycerides for Use in Food and Pharmaceuticals
Rarokar, Nilesh Ramesh
Department of Pharmaceutical Sciences Rashtrasant Tukadoji Maharaj Nagpur University Nagpur (M.S.), India 440033
Menghani, Sunil
Department of Pharmaceutical Sciences Rashtrasant Tukadoji Maharaj Nagpur University Nagpur (M.S.), India 440033
Kerzare, Deweshri
Department of Pharmaceutical Sciences Rashtrasant Tukadoji Maharaj Nagpur University Nagpur (M.S.), India 440033
Khedekar, Pramod Bhujangrao
Department of Pharmaceutical Sciences Rashtrasant Tukadoji Maharaj Nagpur University Nagpur (M.S.), India 440033
Glycerides are lipid esters of the glycerol molecule and fatty acids. Their primary function is the storage of energy. Due to its structure and properties, glycerol participates in the formulation or synthesis of many compounds such as food products, cosmetics, pharmaceuticals, liquid detergents. Monoglycerides (MGs) can be formed by both industrial chemical glycerolysis and biological or enzymatic processes. Chemical glycerolysis bring issues of low MGs yield, high operating temperature, formation of undesirable by-products and high energy consumption. On the other hand enzymatic processes have advantages of mild reaction conditions and high purity of MGs. But, several purification steps are required to obtain food or pharmaceutical grade MG, such as neutralization of the reaction media and discoloration followed by expensive molecular distillation. The purpose of this article is to review the main challenges in the synthesis of MGs from triglycerides (TGs) contained in the various fixed oils and application thereof in the food and pharmaceuticals.
Keywords: Catalyzation, glycerolysis, molecular distillation, monoglycerides, solid catalysts.
Fakultas Farmasi, Universitas Gadjah Mada
2017-11-21 10:19:20
application/pdf
https://journal.ugm.ac.id/jfps/article/view/30449
Journal of Food and Pharmaceutical Sciences; Vol 5, No 2 (2017): J. Food Pharm. Sci (May-August)
eng
Copyright (c) 2017 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/30456
2017-11-21T04:06:42Z
jfps:ART
"171121 2017 eng "
2339-0948
2089-7200
dc
Biochemistry and Bioactive Compounds on Bamboo Shoots as the Main Component in Lumpia Semarang
Ainezzahira, Ainezzahira
Study program of Food Technology, Faculty of Biosciences, Universitas Surya Unity Tower building, Summarecon Serpong, Tangerang, Banten, Indonesia 158101
Mudhita, Cynthia
Study program of Food Technology, Faculty of Biosciences, Universitas Surya Unity Tower building, Summarecon Serpong, Tangerang, Banten, Indonesia 158101
Giovani, Ivana
Study program of Food Technology, Faculty of Biosciences, Universitas Surya Unity Tower building, Summarecon Serpong, Tangerang, Banten, Indonesia 158101
Buntoni, Metta Santika
Study program of Food Technology, Faculty of Biosciences, Universitas Surya Unity Tower building, Summarecon Serpong, Tangerang, Banten, Indonesia 158101
Magdasari, Junita
Study program of Food Technology, Faculty of Biosciences, Universitas Surya Unity Tower building, Summarecon Serpong, Tangerang, Banten, Indonesia 158101
Semarang style lumpia (or spring roll) is a typical culinary in Semarang city that uses bamboo shoots as filling. The objective of this study is to determine biochemical contents and bioactive compounds in bamboo shoots as the main filling component of Semarang Style Lumpia. This journal will discuss bamboo shoots in terms of the physical characteristics, chemistry, nutrition, sensory, bioactive compounds that exist in them. The result shows that bamboo shoots have physical characteristics, i.e soft texture, crunchy, and generally yellow. The chemical compositions of raw bamboo shoots per 100 grams are 2.6% of protein, 0.3% of fat, 5.2% of carbohydrate, and 91% of water. Bamboo-shoots are sources of food that have low fat but high fiber and minerals so bamboo shoot can be a source of healthy food. Bamboo shoots also contain 17 amino acids, 8 of which are essential for human body. The flavors found in bamboo shoots are influenced by total sugar content, amino acids, and tannins. In general, bamboo shoots have a spicy flavor that comes from cyanide. Bamboo-shoots contain some phytochemical groups that have roles for health, one of them is phenolic compounds. These compounds provide positive health effects such as anti-oxidants, anti-inflammatory, anti-allergy, and anti-microbial. The antioxidant type of phthiocerol on bamboo shoots can lower cholesterol and protect from free radicals.
Keywords : Biochemistry, Bioactive, Characteristic, Semarang Style Lumpia, Bamboo Shoots.
Fakultas Farmasi, Universitas Gadjah Mada
2017-11-21 11:06:42
application/pdf
https://journal.ugm.ac.id/jfps/article/view/30456
Journal of Food and Pharmaceutical Sciences; Vol 5, No 3 (2017): J. Food Pharm. Sci (September-December)
eng
Copyright (c) 2017 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/30461
2017-11-21T04:06:42Z
jfps:ART
"171128 2017 eng "
2339-0948
2089-7200
dc
Investigation of Effect of Extensively Used Polymers on Thermoreversible Properties of Pluronic ® Tri-Block Polymer
Rarokar, Nilesh Ramesh
Department of Pharmaceutical Sciences Rashtrasant Tukadoji Maharaj Nagpur University Nagpur (M.S.), India 440033
Saoji1, Suprit D
Department of Pharmaceutical Sciences Rashtrasant Tukadoji Maharaj Nagpur University Nagpur (M.S.), India 440033
Khedekar, Pramod
Department of Pharmaceutical Sciences Rashtrasant Tukadoji Maharaj Nagpur University Nagpur (M.S.), India 440033
This investigation presents a study on the effect of various polymers on gelling properties of tri-block (Pluronic®) copolymers and increasing the stability parameter of in situ gelling system by altering their composition. The tri-block copolymers finds their importance in fabrication of in situ gelling system for the delivery of various kinds of drugs, which can be administered by topical, ophthalmic or parenteral routes. Pluronic®, is a category of non-toxic, water soluble, biodegradable poly (ethylene oxide)/poly (propylene oxide)/poly ethylene oxide), tri-block copolymers which have application in formulation of various in situ gelling systems. This formulation undergo thermo-reversible gelation, where it exists as a free flowing liquid at low temperature and gels in the range of body temperature to form stable depot in aqueous environment. Gelling system was prepared according to the 'Cold Method' using different concentration of polymers (15% to 20% w/v) and subjected to the determination of gelation temperature (GT), viscosity study and effect of various polymers on the strength of gelation. Overall study on the gelation of system at particular temperature is the important parameter for formulation of in situ drug delivery system. It was established that addition of 0.5% w/v of HPMC K4M into gelling system make it stable for forming gel in the range of body temperature whereas methyl cellulose, carbopol 934P, and HPMC E-5 restrict the gel formation.
Key words: Tri-Block Copolymer; Gelation Temperature; Gelling System; Depot.
Fakultas Farmasi, Universitas Gadjah Mada
2017-11-21 11:06:42
application/pdf
https://journal.ugm.ac.id/jfps/article/view/30461
Journal of Food and Pharmaceutical Sciences; Vol 5, No 3 (2017): J. Food Pharm. Sci (September-December)
eng
Copyright (c) 2017 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/30466
2017-11-21T04:06:42Z
jfps:ART
"171110 2017 eng "
2339-0948
2089-7200
dc
Piptadenia Africana: Enhances Weight Gain, Oxidative Stress, Hyperlipidemia in Normal and Hypercholesterolemic Male Wistar Rat
Onyenibe, Nwozo Sarah
Nutrition and Industrial Biochemistry Unit, Biochemistry Department, FBMS, College of Medicine, University of Ibadan, Ibadan, Nigeria
Emmanuel, Oyinloye Babatunji
Nutrition and Industrial Biochemistry Unit, Biochemistry Department, FBMS, College of Medicine, University of Ibadan, Ibadan, Nigeria
Department of Biochemsitry, College of Science, Afe Babalola University, Ado Ekiti, Nigeria
Poyphenol rich stem bark of Piptadenia africana (PA) is used to treat gastric pain, fever, cough and we evaluated the effect of daily, continual intake of PA for eight weeks in hypercholesterolemic rats. Thirty six rats were divided into six equal groups, A (positive-control); B (cholesterol) (negative-control); C (100 mg/kg bwt PA); D (200 mg/kg bwt PA); E (cholesterol+100 mg/kg bwt PA) and F (cholesterol+200 mg/kg bwt PA). Corn oil served as vehicle for both cholesterol (40 mg/kg/0.3ml) and PA. PA caused increased weight-gain, no enlarged organs but decreased their protein concentrations relative to control. Serum triglyceride, total cholesterol and LDL-cholesterol were elevated in PA only and PA co-treated cholesterol rats relative to both control groups, culminating in high atherogenic index. Lipidperoxidation increased dose dependently while glutathione-peroxidase, glutathione-S-transferase, reduced glutathione, superoxide dismutase and catalase decreased in PA treated groups relative to controls. Histological examination revealed necrosis of hepatocytes in groups D and moderate coagulation of necrosis of tubules of renal medulla in C. Continuous usage of PA may not be totally safe as it enhances weight gain, unhealthy lipid profile, increase chances of cardiovascular disease, elicits oxidative stress and induces organ toxicity.
Key words: atherogenic-index, lipidperoxidation, lipid profile, organ toxicity, Piptadenia africana.
Fakultas Farmasi, Universitas Gadjah Mada
2017-11-21 11:06:42
application/pdf
https://journal.ugm.ac.id/jfps/article/view/30466
Journal of Food and Pharmaceutical Sciences; Vol 5, No 3 (2017): J. Food Pharm. Sci (September-December)
eng
Copyright (c) 2017 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/33521
2019-04-25T04:01:40Z
jfps:ART
"181106 2018 eng "
2339-0948
2089-7200
dc
Renoprotective Effect of Agmatine Against Cyclosporin A- Induced Nephrotoxicity in Rats
El-Kahef, Dalia Hassan
Faculty of Pharmacy, Mansoura University
El-kenawi, Asmaa
Faculty of Pharmacy, Mansoura University
Suddek, Ghada
Faculty of Pharmacy, Mansoura University
Salem, Hatem
Faculty of Pharmacy, Mansoura University
In this study, the modulator effect of agmatine on the oxidative nephrotoxicity of cyclosporin (CsA) in the kidneys of rats was investigated by determining indices of lipid peroxidation and the activities of antioxidant enzymes, as well as by histological analyses. Furthermore, the effect of agmatine on CsA induced hypersensitivity of urinary bladder rings to ACh was estimated. Twenty-four male Sprague-Dawley rats were randomly divided into three groups, namely control, CsA and CsA plus agmatine. At the end of the study, all rats were sacrificed and then blood, urine samples and kidneys were taken. CsA administration caused a severe nephrotoxicity which was evidenced by an elevation of serum creatinine, blood urea nitrogen, serum lactate dehydrogenase and protein in urine with a concomitant reduction in serum albumin and creatinine clearance as compared with the controls. Moreover, a significant increase in renal contents of malondialdehyde, myeloperoxidase and tumor necrosis factor-alpha together with a significant decrease in renal reduced glutathione, superoxide dismutase activities and nitric oxide content was detected upon CsA administration with increasing the sensitivity of isolated urinary bladder rings to ACh. Agmatine protected kidney tissue against the oxidative damage and the nephrotoxic effect caused by CsA treatment. In addition, agmatine significantly reduced the responses of isolated bladder rings to ACh. The results from our study indicate that agmatine supplement attenuates CsA -induced renal injury via the amelioration of oxidative stress and inflammation of renal tubular cells. Exposure to CsA caused vacuolated tubular cells and thickened wall vessels, which was found to be prevented by agmatine concurrent treatment. Our study indicates that agmatine administration with CsA attenuates oxidative-stress associated renal injury by reducing oxygen free radicals and lipid peroxidation and inhibiting inflammatory mediators such as TNF-α.
Keywords: CsA; Agmatine; Nephrotoxicity; Urinary bladder; TNF-α; Nitric oxide
Fakultas Farmasi, Universitas Gadjah Mada
2018-11-06 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/33521
Journal of Food and Pharmaceutical Sciences; Vol 6, No 2 (2018): J. Food Pharm. Sci (May-August)
eng
Copyright (c) 2018 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/35739
2018-05-23T01:59:02Z
jfps:ART
"180124 2018 eng "
2339-0948
2089-7200
dc
Enzymes Involving in Chocolate Processing
Kiyat, Warsono El
Department of Nutrition and Food Technology, Faculty of Life Sciences, Surya University, Jl. MH Thamrin KM. 2.7, Tangerang, Banten 15143, Indonesia
Monica, Audrey
Department of Nutrition and Food Technology, Faculty of Life Sciences, Surya University, Jl. MH Thamrin KM. 2.7, Tangerang, Banten 15143, Indonesia
Qomariyah, Noor
Department of Nutrition and Food Technology, Faculty of Life Sciences, Surya University, Jl. MH Thamrin KM. 2.7, Tangerang, Banten 15143, Indonesia
Manurung, Brian Saputra
Department of Biotechnology and Neuroscience, Faculty of Life Sciences, Surya University, Jl. MH Thamrin KM. 2.7, Tangerang, Banten 15143, Indonesia
Indonesia is one of the largest cocoa-producing countries in the world, but the productivity of Indonesian cocoa is relatively low compared to other cocoa-producing countries. Many cocoa beans are exported in raw form (raw cocoa beans) so they do not have high economic value. In addition, cocoa beans also can not be consumed directly and must be processed first. The processing of cocoa beans includes curing stage, breaking the fruit, fermentation, soaking and washing, drying, sorting, and storage to producing cacao almonds. The fermenting process of cocoa beans involves the role of the polyphenol oxidase to produce flavor precursor compounds. Furthermore, to improve the quality of the resulting chocolate, protease and carboxypeptidase can be used in the processing of cacao almonds to enhance flavor precursor compounds forming to produce chocolate with stronger flavor characteristics. Processed chocolates can be developed into confectionery with a soft or liquid filler by utilizing invertase to obtain a solid filler during processing that may turn into liquid during storage.
Keywords : cocoa, chocolate, enzyme, processing.
Fakultas Farmasi, Universitas Gadjah Mada
2018-05-23 08:59:02
application/pdf
https://journal.ugm.ac.id/jfps/article/view/35739
Journal of Food and Pharmaceutical Sciences; Vol 6, No 1 (2018): J. Food Pharm. Sci (January-April)
eng
Copyright (c) 2018 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/35743
2018-05-23T01:59:02Z
jfps:ART
"180303 2018 eng "
2339-0948
2089-7200
dc
Subchronic Toxicity Test of Kebar Grass Extract of n-Hexane (Biophytum Petersianum Klotzsch) on Weight, ADG and Vital Organ Weight of Rat (Rattus Norvegicus)
Sambodo, Priyo
Faculty of Animal Husbandry, Papua University
Nurhayati, Dwi
Faculty of Animal Husbandry, Papua University
Purwaningsih, Purwaningsih
Faculty of Animal Husbandry, Papua University
Susmiati, Trini
Department of Biochemestry, Faculty of Veterinary Medicine, Gadjah Mada University of Yogyakarta
Airin3, Claude Mona
Department of Physiology, Faculty of Veterinary Medicine, Gadjah Mada University of Yogyakarta
The recent study was carried out to determine the effect of Kebar grass extract of N-hexane (Biophytum petersianum Klotzsch) for 28 days peroral on weight, ADG and weight of vital organs of white rat. Forty male and female rats of Sprague Dawley strain aged 8 weeks were examined. The formulated rats were divided into four groups, group I (control), group II, III, IV (treatment), in which each consisted of five rats. The feed were given as much as 15 g/rat/day, while drinking ad libitum. In the control group, the rats were given solvent extract, group II, III and IV were given fraction of n-hexane of Kebar grass extract at 1 mg/kg BW, 5 mg/kg BW and 10 mg/kg BW peroral, respectively. Test preparation was given daily for 28 days, observation of toxic symptoms was performed every day while weighing was performed once every two weeks. Based on results obtained in this line of research, the findings indicated that Kebar extract administration for 28 days did not cause toxic symptoms in both male and female rats either in dose II, III or IV. Weight and ADG of male and female rats did not indicate significant differences between the control group and the treatment group.
Keywords: kebar grass, rats, subcronic toxicity test, OECD 407
Fakultas Farmasi, Universitas Gadjah Mada
2018-05-23 08:59:02
application/pdf
https://journal.ugm.ac.id/jfps/article/view/35743
Journal of Food and Pharmaceutical Sciences; Vol 6, No 1 (2018): J. Food Pharm. Sci (January-April)
eng
Copyright (c) 2018 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/37324
2019-04-25T04:01:40Z
jfps:ART
"181106 2018 eng "
2339-0948
2089-7200
dc
Monitoring of Some Chemical Changes in Turkish Uslu Monocultivar EVOO During 12 Months of Storage
Ghanbari Shendi, Esmaeil
Department of Food Engineering, Hacettepe University, Ankara, Turkey https://orcid.org/0000-0002-6086-0956
Ozay, D. Sivri
Department of Food Engineering, Hacettepe University, Ankara, Turkey
Ozkaya, M. T.
Ankara University, Department of Horticulture, 06110 Ankara, Turkey
Ustunel, N. F.
Nar Doğal Ürünler Tur. Tic. San. A.Ş., 34770 Ümraniye-Istanbul, Turkey
In this research, a Turkish olive cultivar named as “Uslu” locally grown in Akhisar was used for production of monocultivar extra virgin olive oil (EVOO) by using Mobile Olive Oil Processing Unit” (TEM Oliomio 500-2GV, Italy). Olive oil samples were bottled and stored up to 12 months. Some chemical properties such as free fatty acid content, peroxide value, moisture content and UV absorbance value, minor and major components (tocopherols, total phenol compounds and phenolic composition), were determined during storage for 12 months. Chemical parameters such as free fatty acid content, peroxide value of “Uslu” olive oil samples were in agreement with the trade standards of International Olive Council (IOC). Results showed that color values of EVOO changed from green to yellow. UV absorbance values altered during storage. EVOO samples had very low free fatty acidity (0.2%) values which are unusual for commercial olive oils at the end of storage time. Uslu EVOO samples had high content of total phenols (359.33 ppm) and α-tocopherol (265.80 ppm). These values decreased approximately 25.37-15.48 % at the end of storage, respectively. Luteolin was the most abundant phenolic compound and its concentration decreased from 289.54 to 257.00ppm during storage. Results showed that tocopherol isomers, total phenols and phenolic compounds content decreased during 12 months storing.
Fakultas Farmasi, Universitas Gadjah Mada
2018-11-06 00:00:00
application/pdf
https://journal.ugm.ac.id/jfps/article/view/37324
Journal of Food and Pharmaceutical Sciences; Vol 6, No 2 (2018): J. Food Pharm. Sci (May-August)
eng
Copyright (c) 2018 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/41330
2020-02-27T02:33:51Z
jfps:ART
"200227 2020 eng "
2339-0948
2089-7200
dc
The Characteristic of Lada Powder wrapping with various of packaging (biodegradable film of composite film from Sodium CarboxymethylCellulose which syntesized from Pineapple crown, Biodegradable film of Commercial Sodium CarboxymethylCellulose, and Plastics) during storages
Susana, Susana
Pontianak State Polytechnic https://scholar.google.co.id/citations?user=h-dW95UAAAAJ&hl=en
Mangunsong, Lamria
Pontianak State Polytechnic
Iskandar, Dodi
Pontianak State Polytechnic
Purwandani, Ledy
Pontianak State Polytechnic
ABSTRACT
This study aimed to evaluate the use of the composite film Sodium Carboxymethyl Cellulose on the crown of the pineapple to volatile substances of Lada powder, include piperin content (HPLC), Steps being taken in this research is the manufacture of composite films NaCMC and application in Lada powder. The stages in this research are cellulose extraction step and cellulose modification into CMC, making and characterizing composite film as spice packing and its application as spice packer. Cellulose extraction from pineapple crown with 12% NaOH and carboxymethylation with sodium monocloroacetate. Films of sodium carboxymethylcellulose, polyethylene glycol 400 as plasticizer and emulsifier and coconut oil as hydrophobic component to obtain composite film as a spice packing with mechanical properties such as seal strength, thickness, WVTR and solubility in hot water suitable for application as spice packing with thickness 0.085mm) and 350ºC sealing temperature variations. The result of the study of carboxymethyl group of pineapple crown CMC synthesis with HPLC showed that plastic packaging is best compared to using CMC comercial and pineapple crown to lada powder wrapping.
Fakultas Farmasi, Universitas Gadjah Mada
2020-02-27 09:33:52
application/pdf
https://journal.ugm.ac.id/jfps/article/view/41330
Journal of Food and Pharmaceutical Sciences; Vol 6, No 3 (2018): J. Food Pharm. Sci (Sept-December) In Press
eng
Copyright (c) 2020 Journal of Food and Pharmaceutical Sciences
oai:jurnal.ugm.ac.id:article/42724
2020-02-27T02:33:51Z
jfps:ART
"200227 2020 eng "
2339-0948
2089-7200
dc
Chemical Characterisation, Organic Acids by HPLC, Fatty Acids by GC-GCMS and Antioxidant Activity of Commonly Consumed Leafy Vegetables in India
Pamidighantam, Prabhakara Rao
CSIR CFTRI Resource Centre, Habshiguda Uppal Road, Hyderabad 500007
Chemical, organic acids, fatty acid composition and antioxidant activity of commonly consumed leafy vegetables such as Hibiscus cannabinus, Rumex vesicarius, Basella rubra and Alternanthera sessilis were investigated. High performance liquid chromatography (HPLC) was used to quantify organic acids in leaf powders. Protein and fibre contents of Alternanthera sessilis leaf powder were the highest (31.2, 12.5% respectively) and lowest in Hibiscus cannabinus leaf powder (20.86 and 3.94%) among the leaf powders analysed. Oxalic acid was found to be the dominant acid in all the leafy vegetable powders and it was maximum in Alternanthera sesilis (10733 mg/100g) and Basella rubra (23331 mg/100g) powders. Gas and mass chromatography (GC-MS) analysis of leaf powder lipids were rich in palmitic (14.6 – 24.2%), linolenic (25.6 -56.9%), linoleic (15.4-21.1%) and oleic (2.6 - 13.7%) acids. Hibiscus leaf powder exhibited maximum inhibition of 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical with 95% inhibition at 2.8 mg/ml concentration and assay 92% of ABTS (2, 2-Azinobis-3-ethyl Benzothiazoline-6-Sulfonic acid) diammonium salt) at 0.6mg/ml concentration.
Fakultas Farmasi, Universitas Gadjah Mada
2020-02-27 09:33:52
application/pdf
https://journal.ugm.ac.id/jfps/article/view/42724
Journal of Food and Pharmaceutical Sciences; Vol 6, No 3 (2018): J. Food Pharm. Sci (Sept-December) In Press
eng
https://journal.ugm.ac.id/jfps/article/download/42724/115961
Copyright (c) 2020 Journal of Food and Pharmaceutical Sciences