2024-03-29T05:32:32Z
https://journal.ugm.ac.id/index/oai
oai:jurnal.ugm.ac.id:article/2949
2015-11-18T02:04:30Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2949
2015-11-18T02:04:30Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 04 (2009)
Electrocardiographic pattern changed in hospitalized leptospirosis and its association with disease severity
Bambang Irawan, Anggoro Budi Hartopo
2015-11-18 09:04:30
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2949
id
Background: Electrocardiographic (ECG)changes have been observed in leptospirosis. The prevalence of ECG alterations may reach 80%. Despite of this finding,few studies have been conducted to assess ECGpatterns in only hospitalized leptospirosis patients in Indonesia. Objective: To reveal the prevalence and type of ECGalterations in hospitalizedleptospirosisand its association with disease severity. Methods: This was a cross-sectional study using medical record data. Patients hospitalized in Dr. Sardjito Hospital (January 2003 - December 2007) with leptospirosis (lCDXA27.9) were enrolled. Electrocardiogramstaken in 24 hours of admission were evaluated. Disease severity was determined based on clinical and laboratory findings. Association between disease severity with ECGrecordings were performed using Kruskal-Wallisand Chi square test. P value < 0.05 was significant. Results: Sixtysamples were analyzed.The prevalence of alterationof ECGrecordingswas 66.7%. Sinustachycardia was the most frequent (36.7 %). The most common arrhythmia was atrial fibrillation(10 %). Other abnormalities were alterationof ventricularrepolarization(8.3%1.extrasystoles (6.6%) and disorderof conduction (4.9%). Although not significant, those with atrial fibrillationtended to have the worst clinicaland laboratory findings. Conclusions: The prevalenceof alterationof ECGrecordingsin hospitalizedleptospirosiswas 66.7 %. Atrialfibrillation was the most frequent arrhythmia, and patients with this arrhythmia were likelyto have the most severe disease.
oai:jurnal.ugm.ac.id:article/2950
2015-11-18T02:04:30Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2950
2015-11-18T02:04:30Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 04 (2009)
Serum iron level after ingestion of repeated dose of iron shortly after and 2 hours after meal
Sri Kadarsih Soejono, Lukman Hakim, Erna Kristin, Mohammad Hakim,
2015-11-18 09:04:30
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2950
id
Background: The prevalence of iron deficiency anemia in pregnant women is estimated to be higher than nonpregnant women. Ironsupplementation program has been proven clinicallyto improvethe hemoglobin (Hb)level of pregnant women. The absorption of iron is affected by food, therefore iron has to be given 2 hours after meal. However, in practice, iron was given shortly after meal. The physiologicalchange in pregnant women affects drug absorption, distribution, and eliminationphases. Objective: To understand serum iron level after ingestion of repeated dose of iron shortly after and 2 hours after meal for 12 weeks in trimester 2 pregnant women with iron deficiency anemia. Method: The research design was a phase IIclinical trial. Subjects were 24 trimester 2 pregnant women with iron deficiency anemia, classified into two groups, who were treated as follows: The first group was consisted of 12 women with irondeficiency anemia, treated with twice-a-day ferrous sulphate tablet @300 mg orally,given shortly after meal for 12 weeks; the second group was consisted of 12 women with iron deficiency anemia, treated with twice-a-day ferrous sulphate tablet @300 mg orally, given 2 hours after meal for 12 weeks. Bloodsamples were taken in week 2, 4, 6, 8, 10, and 12 after treatment. Serum (ferric) iron level was measured with Vitros Fe Slides method. Result: Minimum,maximum, and average steady-state ironlevels (Css min, Css max, Css average) of treatment 1 were 104.1 ::I:14.03 ug/dL, 96.44::1:13.22 ug/dL, and 112.38::1:14.03 ug/dL (mean ::I:SEM),respectively; while minimum, maximum, and average steady-state iron levels (Css min, Css max, Css average) of treatment 2 were 125.77::1:9.31 ug/dL, 118.03::1:9.21 ug/dL, and 125.77::1:9.31 ug/dL (mean::l:SEM),respectively. No statistical significant difference was found within treatment in minimumsteady-state level between week 2, 4, 6, 8, 10, 12 after treatment. There was also no significant difference in minimumsteady-state level between treatment groups in week 2, 4, 6, 8, 10, and 12. Conclusion: There were no differences in serum iron level after ingestion of repeated dose of ironshortly after and 2 hours after meal for 12 weeks intrimester 2 pregnant women with iron deficiency anemia.
oai:jurnal.ugm.ac.id:article/2951
2015-11-18T02:04:30Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2951
2015-11-18T02:04:30Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 04 (2009)
Atypical carcinoid of the lung: A case report
Soeripto;, J Bras, Hanggoro Tri Rinonce;, Toddy Guntersah;,
2015-11-18 09:04:30
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2951
id
A 3D-year old woman came to Dr Sardjito Hospital with chief complain of dyspneu that she had since 1 month before her visit. Clinicalexamination revealed a dull percussion below. The second intercostal of the left chest. The chest X ray revealed massive pleural effusion in the left chest, could not exclude a mass in left lung. Thoracoscopic biopsy of the left lung mass was done. Macroscopic examination of lung tissue sample revealed fragmented tissue, with volumeof 0.25 cc, blackcolour, and rubberyconsistency. The diagnosis of atypicalcarcinoid with small cell carcinoma as a differential diagnosis was determined based on morphological pattern. Immunohistochemical staining examination showed expression of cytokeratin, CAM5.2, and synaptophysin, but showed negative expression of chromogranin, CD56, and TTF-1 .A diagnosis of an atypical carcinoid was confirmed based on the immunohistochemicalexamination. A very rare case of atypical carcinoid of the lung in a non-smoker 3D-yearoldwoman with morphologicaldiagnostic problem solved by immunohistochemical staining was reported.
oai:jurnal.ugm.ac.id:article/2952
2015-11-18T02:04:30Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2952
2015-11-18T02:04:30Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 04 (2009)
Fibroadenoma of the breast with florid epithelial cells hyperplasia: a case report
Soeripto;, J.L. Peterse, Irianiwati;, Rahmayani;, E. Soekarti;
2015-11-18 09:04:30
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2952
id
Fibroadenoma is a benign biphasic tumor of the breast, composed of an epithelial and a stroma component. Fibroadenoma with epithelial cells hyperplasia may contribute to the higher risk of breast cancer, especially for florid and atypical hyperplasia. The distinction between fibroadenoma with florid epithelial cells hyperplasia, in-situ carcinoma and invasive duct carcinoma of the breast ban be difficult morphologically. To solve the problem, immunohistochemical staining with specific andibodies will be helpfull in distinguishing usual duct hyperplasia from ductal carcinoma in situ. A case of fibroadenoma with florid epithelial cells hyperplasia has been reported. Morphologically, this tumor is difficult to be differentiated from ductal carcinoma. Immunohistochemical staining with High molecular weight cytokeratins 34BE12, Smooth Muscle Actin (SMA),E-cadherin could be used to determine the diagnosis of this tumor.
oai:jurnal.ugm.ac.id:article/2953
2015-11-18T02:04:30Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2953
2015-11-18T02:04:30Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 04 (2009)
The risk factor of urinarytract infection in patient with leukemia
Pungky Ardani Kusuma, Melati Dini Hari, Soetaryo,
2015-11-18 09:04:30
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2953
id
Background: Urinary tract infection (UTI) is one of most common infectious diseases in patients wuth leukemia. Some researchers showed that the prevalence of UTI was the third highest after pneumonia and gastrointestinal tract infection among children with leukemia. UTI may be affected by many factors, i.e. host, microorganism, and environment. E. coli, Klebsiella, dan Proteus are the most common bacterial etiology of UTI in leukemia. Objective: The aim of this research was to know the risk 'factor of UTI in children with leukemia. Method: This research used case-control design. Subjects of this study were children (0-15 years old) with leukemia who came to Department of Pediatrics, Dr. Sardjito Hospital since January st, 2000 until October 3", 2008. There were 38 patients in case group and 109 patients in the control group. Data collected from medical records were age, gender, nutritional status, type of leukemia, treatment phase of leukemia, and absolute neutrophil count. Risk factors analyzed were age, gender, nutritional status, and neutropenia. UTI was diagnosed by bacterial culture and bacterial count. Result: Univariate analysis showed that female gender was the risk factor of UTI (OR 2.35; CI 1.10- 5.00), meanwhile age less than 5 years old (OR 0.48; CI 0.22-1.06L poor-fair nutritional status (OR 1.20; CI 0.52-2.78), and neutropenia (OR 0.73; CI 0.35-1.55) were not risk factors of UTI. Multivariate analysis showed that female gender was the risk factor of UTI (OR 2.36; CI 1.08-5.14). Conclusion: Female gender was a risk factor of UTI in children with leukemia Keywords: leukemia - children - gender - urinary tract infection - risk factors
oai:jurnal.ugm.ac.id:article/2954
2015-11-18T02:04:30Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2954
2015-11-18T02:04:30Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 04 (2009)
The effect of metformin on proliferation and glucose uptake in keloid fibroblast culture
Yohanes Widodo Wirohadidjojo, Nur Dwita Larasati, Sunardi Radiono
2015-11-18 09:04:30
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2954
id
Background: Metformin as an antihyperglycemic agent has a potential effect in increasing type I collagen synthesis and decreasing MMP, so that it has a potential to be an antiaging agent. One of aging failure processes is the development of keloids. Keloids are formed due to hyperproliferation of fibroblasts, an increase of collagen synthesis, particularly type I and III, and a decrease in MMP-1 and MMP-2. Fibroblast proliferation process and collagen synthesis need glucose uptake. The study on metformin ability to aggravate or stimulate the formation of keloid has never been conducted before. Objective: The aim of this study was to know the difference of proliferation and glucose uptake between keloid fibroblasts given metformin and without metformin. Method: A simple experiment was conducted using 3rdpassage keloid fibroblasts culture. Keloid fibroblasts were divided into 2 groups, the first group was treated with metformin in the dose of 100 pg/mL, 200 pg/mL, 300 pg/ mL, 400 pg/mL, and control. Keloid fibroblasts proliferation in the first group was measured using spectrophotometer with MTT assay, and glucose uptake of keloid fibroblast in the other group was measured using glucometer. The difference in proliferation and glucose uptake of keloid fibroblast was analyzed using one-way anova. Result: The result of this study showed that the average keloid fibroblast proliferation in the metformin treatment groups was not increased compared to that in control group. Meanwhile, the average keloid fibroblast glucose consumption in metformin treatment group significantlyincreased, at the dose of 300 ig/mL (p =0.044) and 400 I!g/mL (p = 0.0081. Conclusion: Metformin could not increase keloid fibroblasts proliferation, but it could increase glucose uptake of keloid fibroblasts.
oai:jurnal.ugm.ac.id:article/2955
2015-11-18T02:04:30Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2955
2015-11-18T02:04:30Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 04 (2009)
The effect of zinc supplementation on collagen of periodontitisrat
Soeharyo Hadisaputro, Suryono, Praptiwi, Siti Fatimah Muis
2015-11-18 09:04:30
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2955
id
Background: Zinc,cofactors of DNA-and RNA-polymerase, having great role in tissue heal-ing. In periodontitis, collagen type 1 the main fiberconstituting periodontal structure is des-troyed and be the main cause of lost of teeth among adult in Indonesia. Zndeficiency is still a nutritional problem. The aim of the study is to obtain the influence of 500 pg Zn supplementation (Zn,1and 200 pg (Zn2)per day for 7 days to periodontal collagen of Periodontitis (PI rat through histologic feature. Method: inthe experimental study with factorial design, 29 adult male Wistar rat were used. Subjects were grouped simple randomly into 6 i.e Healthy (H)at the start, Hatthe end, p, P+Te-tracycline IT), ,P + T+ Zn" and P+ T+ Zn2.Subject other than Hgroups were induced periodontitisby Porphyromonasgingivalisbacteria. Zn concentration was measured by AAS Flame, Periodontal tissue was stained with Mallory,s. T test and ANOVAwas used to analyse difference between mean of Znconcentration of groups studies. Result: There were similarity in the feature of groups H at the start, Hat the end, and P+ T+ Zn" in sense of regularity, length, and solidity of collagen. P group had irregular, and short collagen. Groups of P+ T with shorter collagen, had similarity to Health at the end. P+ T+ Zn2with shorter collagen similar to P+T+ Znl. There was significant difference inZn concentrat-ion between Hat the end and Pgroup. Nosignificant differencies among Zn concentrations of Psubject groups. Great variety of Zn concentration found among subjects of groups prob-ably were the cause of absence of difference, although means of the Zn concentration values depicted it. Conclusion: Zn supplementation dosage 500 pg/day given to periodontitis rat beside Tetra-cycline, gives better effect to collagen structure compared to 200 pg/day.
oai:jurnal.ugm.ac.id:article/2956
2015-11-18T02:04:30Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2956
2015-11-18T02:04:30Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 04 (2009)
Human sexual dimorphism: from an evolutionary perspective to practical . overview
Suriyanto, Rusyad Adi
2015-11-18 09:04:30
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2956
id
This paper goal is to discuss human sexual dimorphism in evolutionary perspective and practical overview. Evolution of human sexual dimorphism has wide implication !n human biological study and many applications to medical field. Human sexual dimorphism has experienced changes and adaptations along its proceeding to current time and space. These changes are natural consequences. Understanding this problem as process and products of evolution, we can enter this problem into medicine and be a background. Understanding as a background, as human natural history, we will understand why human can be so now and why he! she will change, including sexual dimorphism problem. The understanding of background is needed to avoid creation of human with narrow vision, highly specialized, excessive fanatics for self competency, easily astonished, panic and immediately tricked in real life which is complex and continuously changing. Human anatomy knowledge, as one basic medical aspect, sexually presents understanding that there are changing and developing characteristics among men (males) and women (femalesl over time. The medical intentions are aspects of sexual dimorphism directly applied and related closely to medicine. The paper begins with sexual dimorphism discussion, and continued by evolutionary perspective discussion, and followed up by practical discourse. This continuity is an effort to illustrate that Homo sapiens species, like us, is always dynamic and changing, like nature which is never static neither stable.
oai:jurnal.ugm.ac.id:article/2957
2015-11-18T02:04:30Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2957
2015-11-18T02:04:30Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 04 (2009)
The effect of operating room design and behavior of operating room staff on surgical site infection (881) incidence
Iwan Dwiprahasto, Yulianto Widodo;,
2015-11-18 09:04:30
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2957
id
Introduction: In surgery, medical error is often related to surgical site infection (551).Santa Maria Hospital, Pemalang, is a private hospital which gives priority to quality and applies the patient safety principle. During 2006, there were 7 cases of 551 in 785 surgical cases in Santa Maria Hospital, Pemalang, but this number was considered as the tip of an iceberg. The high microorganism rate, operating room design which was not agreeable with the standard by Kepmenkes No 1204/MENKES/SK/X/2004, and the behavior of operating room staff were the risk factors of 55!. ObJective:To find out the effect of the change in operating room design and behavior of operating room staff on 551 incidence in Santa Maria, Pemalang, and to find out the risk factors of 55!. Method: It was a quasi-experimental study with pre- and post-test without control group design. Population were all operating room staff, while subject of this study was operating room design. Dependent variable was 551incidence, while independent variables were behavior of operating staff and operating room design. Data were analyzed with independent t-test, chi-square test, and multivariate logistic regression. Re.ult: The change in operating room design and operating room staff behavior has significantly decreased the incidence of 551 (OR = 3.646; 95%CI = 1.24 < OR< 10.77). Conclu.lon: Intervention on operating room design and staff behavior has decreased the incidence of 55!. Risk factors of 551were operating room design, operating room staff behavior, and duration of surgery.
oai:jurnal.ugm.ac.id:article/2958
2015-11-18T02:08:53Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2958
2015-11-18T02:08:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 03 (2009)
Optimal temperature for normal human skin fibroblast proliferation and glucose uptake, an in vitro study
Sunardi Radiono, Yohanes Widodo, DeviArtami Susetiati, LailyNoorQomariah
2015-11-18 09:08:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2958
id
Devi Artami Susetiati, Laily Noor Qomariah, Sunardi Radiono,Yohanes Widodo Wirohadidjojo -Optimal temperature for normal human skin fibroblast proliferation and glucose uptake; In vitro study Background: Wrinkles is caused by a decrease in collagen synthesis and an increase in collagen degradation. Collagen synthesis depends on fibroblast proliferation. Collagen synthesis needsglucose, so that collagen synthesis may be expressed by the increase in glucose uptake. Skin rejuvenation with heating may increase the collagen synthesis. The effect of short-term heating and optimal temperature on fibroblast proliferation and glucose uptake has not yet been defined. Objective: This study was aimed to determine the optimal temperature of short-term heating for normal human skin fibroblasts proliferation and glucose uptake. Methods: A simple experimental study was conducted on 3rd passage subculture of normal human skin fibroblasts culture, isolated from 2 patients. Normal human skin fibroblasts with complete DMEM were classified into 5 groups, and then heated for a minute with thermocycler-machine at 36°C, 46°C, 56°C, 66°C, and 72 °C. Those cells were incubated for 7 days in complete DMEM and subsequently fibroblasts proliferation was measured by MTT -assay. Glucose uptake was measured by glucometer Medisafe- Terumo. The differences in glucose uptake and fibroblasts proliferation were analyzed with one-way Anova. Result: Optimal temperature for fibroblasts proliferation was 46°C, and 66°C for collagen synthesis. Conclusion: Skin rejuvenation based on heating could be performed at two different temperatures, each cycle consisted of 66°C on first heating, and seven days later, at 46°C on second heating.
oai:jurnal.ugm.ac.id:article/2959
2015-11-18T02:08:53Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2959
2015-11-18T02:08:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 03 (2009)
Risk factors of neonatal mortality of referred babies with birthweight of 1000- < 2500 grams
Ekawaty Lutfia Haksari, Dian Murni Wardhani, Setya Wandita
2015-11-18 09:08:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2959
id
Background: Almost 16% from all of the total births in the developing countries are low birthweight babies. Low birthweight babies are an important contribution in neonatal mortality and morbidity worldwide, and contribute to almost 70% of the total neonatal deaths. Proportion of the low birthweight babies in the developing countries is 90% of the total low birthweight babies around the world, which is 20 million annually. Mortality of babies weighing 1000-< 2500 grams in Dr. Sardjito Hospital reached 51.4%, and that of referred babies was 74.6%. Objectives: This research was aimed to find out and measure the risk factors that contribute to the mortality of referred low birthweight babies in the neonatal period. Methods: A hospital-based research with case-control study design was done to discover the risk factors that related to the neonatal mortality of the referred babies with birthweight of 1000- < 2500 grams. Data were collected from babies' medical records treated in the Maternal-Perinatal Installation of Dr. Sardjito Hospital in 2005 to 2007. Target population of this research was the mentioned above who died at the age of 0-28 days. Variable analysis to find out the correlation between the risk factors and the mortality used odds.ratio (OR), 95% confidence interval (95% CI), and the significance level with Chi-square test. To measure the risk factors that contributed on the mortality multivariate logistic regression analysis was used. Result: This study involved 190 babies (95 cases and 95 control) weighing between 1000 and < 2500 grams, who were referred to Dr. Sardjito Hospital. Several subjects were excluded, 23 babies because of going home on selfrequest, 24 because of incomplete status, 76 because of severe congenital anomaly, and 34 were extremely low birthweight babies. Sepsis (OR: 5.054, 95%CI2.433-11.198), HMO(OR:4.461, 95%CI1.595-10.497), hypoglycemia (OR: 3.358, 95%CI1.679-6.715), and hypothermia (OR: 2.221, 95%CI1.082-4.560) were the risk factors of mortality. Conclusion: Sepsis, HMO, hypoglycemia, and hypothermia increased the mortality risk of babies weighing 1000-< 2500 grams referred to Dr. Sardjito Hospital, Yogyakarta. Key words: low birthweight baby -risk factor -referral -neonatal death
oai:jurnal.ugm.ac.id:article/2960
2015-11-18T02:08:53Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2960
2015-11-18T02:08:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 03 (2009)
Serum iron level shortly after iron supplementation shortly after and 2 hours after meal in women with iron deficiency anemia
Sri Kadarsih Soejono,,Lukman Hakim, ErnaKristin, Muhammad Hakimi
2015-11-18 09:08:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2960
id
Background: Incidence of anemia in women in developing countries is still high, that is, around 43%. This incidence rate is far more hig'her that that in industrial countries, which is ranged between 10-12 %. The prevalence of iron deficiency anemia is still high, particularly in developing countries. The cause of the high prevalence of iron deficiency anemia is not known, since it involves various factors. Two of the probable etiologic factors is variability in dosage administration, and the effect of co-administered food. Studies on the pharmacokinetic 'of iron after single dose iron tablet administration in women with anemia and pharmacokinetic of iron coadministered with food in healthy women have been done, but study on repeated dose has never been conducted. Objective: To understand serum iron level after ingestion of repeated dose of iron shortly after and 2 hours after meal for 12 weeks in women with iron deficiency anemia. Method: The research design was a fase II clinical trial. Subjects were 24 women with iron deficiency anemia, classified into two groups, who were treated as follows: the first group was consisted of 12 women with iron deficiency anemia, treated with twice-a-day ferrous sulphate tablet @ 300 mg orally, given shortly after meal for 12 weeks; the second group was consisted of 12 women with iron deficiency anemia, treated with twice-a-day ferrous sulphate tablet @ 300 mg orally, given 2 hours after meal for 12 weeks. Blood samples were taken in week 2,4, 6, 8, 10, and 12 after treatment. Serum (ferric) iron level was measured with Vitros Fe Slides method. Result: Minimum, maximum, and average steady-state iron levels (Css min, Css max, Css average) of treatment 1 were 108,78:t 13.79 ug/dL, 121 .44:t 15.79 ug/dL, and 115.11 :t 13.13 ug/dL (mean:t SEM), respectively; while minimum, maximum, and average steady-state iron levels (Css min, Css max, Css average) of treatment 2 were 115.15 :t 46.27 ug/dL, 141.36:t 61.36 ug/dL, and 124.92:t 53.43 ug/dL (mean:t SEM), respectively. No statistical significant difference were found within treatment in minimum steady-state level between week 2, 4, 6, 8, 10, 12 after treatment. There was also no significant difference in minimum steady-state level between treatment group in week 2, 4, 6, 8, 10, and 12. , Conclusion: There were no differences inserum iron level after ingestion of repeated dose of iron shortly after and 2 hours after meal for 12 weeks in women with iron deficiency anemia. Key Words: iron supplementation-serum iron-iron deficiency anemia-steady-state iron level
oai:jurnal.ugm.ac.id:article/2961
2015-11-18T02:08:53Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2961
2015-11-18T02:08:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 03 (2009)
The effectiveness of rivanol tampon" compared with burowi tampon in acute diffuse otitis externa (ADOE)patients
Bambang Udji Djoko Rianto, I Wayan Marthana Kedel, Edhie Samodra
2015-11-18 09:08:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2961
id
Background: Acute diffuse otitis externa (ADOE) is one of the common diseases in ENT Department, Dr. Sardjito Hospital, with the frequency of 9-1 2%. The most common microorganism found is Pseudomonas aeruginosa. Infection usually occurrs after taking a bath, washing hair, and scratching the external ear canal. Treatment of acute diffuse otitis externa which consists of topical preparations, Le. antibiotics and antiinflammation tampon or ear drop, is relatively expensive. A rationale, high recovery rate, cheap, and easily found preparation is needed. Burowi solution is one of the recommended treatment, but it is less popular. Objective: The goal of this study was to know the effectiveness of Rivanol" tampon compared with Burowi tampon in acute diffuse otitis externa. Methods: A randomized single-blind controlled trial (RCT)was performed at the outpatient clinicof ENTDepartment of DrSardjito General Hospital, Yogyakarta. Sixty-five subjects who were'diagnosed as ADOEwho met inclusion criteria were randomly allocated into two groups, that is, Rivanol"group and Burowi group. The ear canal was cleaned, and then Rivanol"tampon or Burowi tampon was applied. Evaluation was performed on day 3 and 5. Primary outcome was the recovery rate, and secondary outcomes were adverse reaction and marginal costeffectiveness. Results: Of 65 subjects, 33 were alloc'8ted into Rivanol"group and 32 were. allocated into Burowi group. The recovery rate in Rivanol"group was 75.4%, while in Burowigroups was 49.3%. The difference in recovery rate between the two groups was statistically significant (p: 0.018; RR4.350; 95% CI1.225-15.442). Adverse reaction in Rivanol"group was dampness and soggy feeling in the ear canal (1.5%), while in the Burowigroup the adverse events were pain and burnt sensation inthe ear canal (3.1%), but they could be tolerated and disappeared on day 5. Analysis on marginal cost-effectiveness showed that the cost to cure one acute diffuse otitis externa patient with Rivanol"tamponwas Rp 1,000. Conclusion: Rivanol"tampon was more clinicallyeffective and more cost-effective than Burowitampon for acute diffuse otitis externa treatment. Key words: acute diffuse otitis externa, Rivanol(!),Burowi,.clinical and cost effectiveness
oai:jurnal.ugm.ac.id:article/2962
2015-11-18T02:08:53Z
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v2
https://journal.ugm.ac.id/bik/article/view/2962
2015-11-18T02:08:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 03 (2009)
Autoimmune urticaria in Rumah Sakit Umum Pusat Dr. Moh. Hoesin Palembang
Thaha, M. Athuf
2015-11-18 09:08:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2962
id
Background: About 30% patients with chronic idiopathic urticaria (CIU)have circulating histamine-releasing autoantibodies against the a-subunit of high affinity IgEreceptor (FceRla),or IgE.This subgroup of patients has a disorder commonly referred as autoimmune urticaria. Objectives: This study was conducted to reveal the autoimmune urticariacases in Indonesian patients Methods: The autologous serum skin test (ASST)and histamine release assay (HRA)were conducted on 79 patients with CIU (53 females and 26 males). Patients with predominant physical urticaria and urticarial vasculitis were excluded from the study. Results: Seventeen patients had both positive autologous serum skin test and histamine release assay confirmative of autoimmune urticaria. Conclusion:CombinedpositiveASSTand HRAwere seen in 21.5% of CIUpatients, indicatingautoimmune urticaria. Key words: chronic idiopathic urticaria - anti-FceRla histamine-releasing autoantibodies -autoimmune urticaria - autologous serum skin test, histamine release assay.
oai:jurnal.ugm.ac.id:article/2963
2015-11-18T02:08:53Z
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v2
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2015-11-18T02:08:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 03 (2009)
EarlyWarning System (EWS) for Dengue in Indonesia and Thailand
Dana A. Focks, Mohammad Juffrie, Dana A. Focks
2015-11-18 09:08:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2963
id
Background: Dengue virus infection is an acute febrile disease caused by 4 sero-type viruses. The transmission via mosquito vector Ae. Aegypti. The morbidity of dengue virus infection is quite high and the mortality below 5%. The most dangerous form is dengue shock syndrome, the mortality is very high. The effort to reduce morbidity and mortality is improvement of the clinical management and control of vector. Today, most dengue control efforts are based on suppression of Aedes aegypti (L.) and not eradication. EWS would provide significant utility where mitigation methods were available. EWSs were possible for three reasons, an extensive time series on the disease incidence the available, dengue being a vector-borne disease, is significantly influenced by weather, in many sub-regions of SE Asia, weather anomalies are significantly influenced by and lag behind several months, sea surface temperature (SST) anomalies. Methods: Analytic cross sectional study was conducted. The dependant variable in this analysis, Epi.yr. is dichotomous and indicates whether an epidemic occurred during a particular year. The two independent (predictor) variables are sea surface temperature anomalies as reported by the Japanese Meteorological Association (JMA) and previous cases. The monthly number of cases were dengue and DHF in Yogyakarta, Indonesia and the metropolitan area of Bangkok, Thailand. Results: Yogyakarta, many years were very near the epidemic cutoff of 278 cases, yet only one year, 1992 with 237 cases, was incorrectly labeled. The false positive in 1992, had a probability of 0.64 of epidemic and 0.36 of no epidemic. Bangkok, the best three-month prediction gave 6 false indication in 35 years, 5 false negatives, 1 false positive. For two month prediction, 3 errors in 35 years were made, 2 false negatives, 1 false positive. Conclusion: The results presented in this study is very use full for predicting the incidence of dengue virus infection using weather data. This method would only require a simple calculator, or preferably a PC using the derived equation. Key words: dengue -incidence -early warning -weather - probability
oai:jurnal.ugm.ac.id:article/2964
2015-11-18T02:08:53Z
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https://journal.ugm.ac.id/bik/article/view/2964
2015-11-18T02:08:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 03 (2009)
The effect of vitamin C on fibroblast proliferation and VEGFexpression in fibroblast culture
Yohanes Widodo Wirohadidjojo, Munira, Sunardi Radiono
2015-11-18 09:08:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2964
id
Background: One of the factors that determine the success of efficient woul')d healing is wound healing rate, that can be achieved by increasingcell proliferation, angiogenesisor neovascularisation,and extracellular matrix production. Dermalfibroblast is a cell that plays an important role in wound healing. Fibroblast proliferation and neovascularisation are critical elements in granular tissue formation. Local hypoxia causes fibroblasts to express HIF-1a that will induce fibroblast VEGFexpression. The nature of vitamin C makes it easily oxidized. The addition of vitamin Con fibroblast culture medium is expected to produce local hypoxia condition that will induce fibroblast expression of HIF-1a, so that the expression of fibroblast VEGFwill be increased. Vitamin C may modulate the growth of various types of cells. The effect of vitamin Con normal fibroblast proliferation and fibroblast VEGFexpression is still unknown. Objective: This study was aimed to know whether vitamin Ccan increase normal human fibroblast proliferation and expression of VEGF. Method: A simple experimental study was conducted by using preputial skin fibroblast culture from 10-year-old donor, subculture passage 3. Fibroblast culture was divided into 6 groups, each group received vitamin C treatment with the dose of 50pg/mL, 100pg/mL, 150pg/mL, 200pg/mL, and 300pg/mL, and one group without treatment acting as control. Measurement of fibroblast proliferation was conducted by spectrophotometer using MTT, and fibroblast expression of VEGFwas measured using ELISA. The average of difference in fibroblast proliferation and VEGFexpression was analyzed with one-way analysis of variance. Result: There was a significant increase in fibroblast proliferation rate in the groups receiving vitamin C with the dose of 200 mg/mL (p = 0.016) and 300 mg/mL (p = 0.005), whereas in the group with the dose of 50 mg/mL, 100 mg/mL and 150 mg/mL there was no significant difference compared to the control (p = 0.933, p = 0.961, P = 0.301, respectively). Average fibroblast VEGFexpression between various concentrations of vitamin C compared to the control showed no significant difference (p > 0.05). Conclusion: Vitamin Ccould be considered to be used as an agent to accelerate wounds healing. Keywords: vitamin C, skin fibroblast culture, fibroblast proliferation, fibroblast VEGFexpression
oai:jurnal.ugm.ac.id:article/2965
2015-11-18T02:08:53Z
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https://journal.ugm.ac.id/bik/article/view/2965
2015-11-18T02:08:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 03 (2009)
The effect of topically extravirgin olive oil on the UVB-induced immunosuppression
AriefBudiyanto, Irianiwati, Vohanes Widodo Wirohadidjojo
2015-11-18 09:08:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2965
id
Background: UVBradiation may act as an immunosuppressive agent through Langernans cells (LCs)depletion correlated with cyclobuthane pyrimidine dimer (CPD), as the most mutagenic photoproducts. Other studies showed that olive oil can prevent various human cancers, which are defect of immune-surveillance. The effect of olive oil in the UVBinduced LCs depletion is still unclear. Objective: To discover the topical effect of extravirgin-olive-oil in the LCs depletion. Methods: A simple experimental study was performed on foreskins collected from 8 circumcised boys. Each of them was cut into 3 pieces of 0.5 cm2, grouped randomly to: (Olive oil+ UVB)group, (placebo + UVB)group, and unirradiated group. They were cultured in DMEM for 24 hours, irradiated with 100mJs/cm2 UVB, and olive oil or placebo were then applied immediately after irradiation. All specimens were fixed in buffered-formalin, paraffin blocked, and cut to be 2 slides, and each of them was stained with anti-CD 1a or anti-CPD antibody. The pixels of AEC signats were digitally counted based on Photoshop's selected color histogram.The pixel means of various groups were analyzed by Kruskal-Wallis test and post hoc analysis by Mann Whitney test. Results: Olive oil treated groups showed a very significant (P
oai:jurnal.ugm.ac.id:article/2969
2015-11-18T02:04:53Z
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2015-11-18T02:04:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 02 (2009)
The Difference of High Sensitivity-C Reactive Protein in the Diabetic Patient with depression and Diabetic Patiens without Depression
Hamzah Shatri, Deddy N.W.Achadiono, A. Siswanto, E. Mudjaddid
2015-11-18 09:04:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2969
id
Background: Both depression and diabetes mellitus have been related to a higher risk of developing coronary heart disease. Inflammation may be important in the pathogenesis of atherothrombosis. There are many markers of inflammation, one of them is high sensitivity-C reactive protein. Objective: To determine the difference of high sensitivity-C reactive protein in the diabetic patients with depression and in the diabetic patients without depression. Methods: This study used cross-sectional design and examined 64 diabetic patients, aged 30-60 years old. The patients were divided into two groups, Le. with and without depression group.We measured depression using the DSM IV. The diagnosis of diabetes mellitus used PERKENI criteria 2006. We measured the serum concentration of CRP with a high sensitivity assay. Other measured factors included gender, marital status, occupation, blood glucose, HbA 1 c, body mass index, smoking and duration of diabetes. The association between hyperglycemia and hs-CRP were also studied. Results: There were 32 diabetic patients with depression and 32 diabetic patients without depression. Body mass index were corelated with depression in diabetic patients (p = 0.008) but the other factors such as gender, age, marital status, occupation, blood glucose, HbA 1 c, duration of diabetes were not corellated with depression in diabetic patients (p > 0.05). The mean of hs-CRP consentration was higher in diabetic patients with depression (5183.44:t3974.66 vs 2103,17:t1502.94 mg II, p=0.001) than in diabetic patients without depression. There was no association between blood glucose control (HbA 1 c) and hs-CRP concentration. Conclusion: High sensitivity-C reactive protein concentration was higher in diabetic patiens with depression compared with diabetic patients without depression.Key words: hs-CRP - with and without depression diabetes mellitus type II
oai:jurnal.ugm.ac.id:article/2971
2015-11-18T02:04:53Z
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https://journal.ugm.ac.id/bik/article/view/2971
2015-11-18T02:04:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 02 (2009)
The Role of Hearing Aids use on Reading Ability in Deaf Students
Djoko Rianto, Bambang Udji
2015-11-18 09:04:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2971
id
Introduction: Reading is a very complex process, which includes the cognitive process, initialized by visual analysis and letter transformation into vocabulary form, word identification by letter sound mapping, correlating words and interpretation. Deafness causes difficulties in acquiring language information via hearing process thus inhibiting a successful conversation process. Hearing aids can amplify sound information to the auditory organs. Objective: To know the effect of hearing aids on deaf students. Methods: A historical cohort design was done. The reading ability of 35 deaf students with hearing aids who received early education were compared to those without hearing aids. The variables measured included age, age of school enrollment, length of studies, degree of deafness, sex, parental educational level. Results: Regression analysis showed that hearing aids plays a role to significantly increase reading ability statistically (p: 0.006; OR: 10.197; 95% CI: 1.939-53.628) compared to students without hearing aids, besides the length of studies variable (p: 0.002; OR: 38.250: 95% CI: 3.739-391.361). Conclusion: This study concluded that the use of hearing aids significantly play a role to increase reading ability in deaf students who receive early education. Key words: hearing aids - deaf student - reading ability.
oai:jurnal.ugm.ac.id:article/2972
2015-11-18T02:04:53Z
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2015-11-18T02:04:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 02 (2009)
The Role of Cervical Erosion on Vaginal Colonization of Candida sp. Study on the Intrauterine Contraceptive Device Users in Puskesmas Depok I Sleman
Satiti Retno Pudjiati, Erni Setyawati, Fajar Waskito
2015-11-18 09:04:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2972
id
Background: Candida sp is an opportunistic pathogenic yeast causing clinical diseases such as vulvovaginal candidiasis (VVC) under certain circumstances. Availability of erythrocytes in the vagina, as in cervical erosion, creates an ideal niche for yeast possessing erythrocyte-binding surface receptors to enhance colonization of Candida sp. Objective: To compare the number of vaginal Candida sp colonies among IUD users with and without cervical erosion. Methods: It was a cross-sectional study involving 45 IUD users. Subjects were classified into cervical erosion group and without cervical erosion group, who underwent vaginal lavage to obtain vaginal fluid samples. Candida sp were grown by inoculating 0.1 ml of vaginal fluid samples into CEA culture media and incubated at 25°C-30°C for 48 hours. Candida sp would grow as round and smooth colonies in blackish-brown color. The numbers of colonies (cfu/ml) in each CEA disk were measured manually, and multiplicated with the factors of dilution. Data were numeric variables, and analyzed with Student's t-test. Results: Average number of Candida sp colonies in the group with cervical erosion was 154.25:t 102.61 cfulml, while that in the group without cervical erosion was 47.00:t 39.08 cfulml. Conclusion: Average number of Candida sp colonies in IUD users with cervical erosion was significantly higher than in IUD users without cervical erosion (p < 0.05). Key words: cervical erosion - Candida sp - VVC - yeast possessing erythrocyte-binding receptors -IUD
oai:jurnal.ugm.ac.id:article/2973
2015-11-18T02:04:53Z
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https://journal.ugm.ac.id/bik/article/view/2973
2015-11-18T02:04:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 02 (2009)
Body Composition in Javanese Adult: Some Anthropometric Dimensions Related to Body Fat
Hastuti, Janatin
2015-11-18 09:04:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2973
id
Background: Body composition is an integral component of entirely health and physical fitness. Measurement of fat component in assessing body composition is necessary on account of its relation to several health risks. Some anthropometric measurements such as waist to hip ratio (WHR), waist and hip circumferences, skinfold thickness, and sagittal abdominal diameter are demonstrated to be valuable for evaluating body composition, and further for identifying some health risk factors. Objectives: This study is addressed to know body composition and some anthropometric measurements related to percent body fat in Javanese adults living in Yogyakarta province. Methods: As many as 201 (98 males and 103 females) Javanese adults living in Yogyakarta province, aged 18 to 65 years were entrolled in this study. Measurements of body weight, height, waist, abdominal, and hip circumferences, sagittal abdominal diameter, and total skinfold thickness of triceps, biceps, subscapular, suprailiac were performed on the subjects. Body composition was assessed based on those measurements included percent body fat, body density, and skeletal muscle mass. Body mass index and waist-to-hip ratio were determined and categorized. The data then were analysed using independent Hest, Pearson correlation of product moment, linear regression, and chi-square test. Results: The result indicated that in a general sense, Javanese adults in Yogyakarta province had thin to medium body constitution and risk factor categorized of low to medium risk for males and medium to high risk for females. Javanese adult males were considerably heavier, taller, had greater means of body density, WHR, and muscle mass. By contrast, Javanese adult females were significantly higher in BMI, percentage of BF, and hip circumference. Statistically, there is no significant difference in total skinfold, abdominal circumference, and SAD, however, females indicated to a certain greater than the males. In the same manner, males had larger waist circumference. Conclusions: All of anthropometric dimensions including skinfold thickness, body density, waist circumference, abdominal circumference, WHR, sagittal abdominal diameter, and muscle mass are strongly correlated with body composition assessed with BMI and % BF. However, associations with BMI are fairly better than those of with percentage of BF. Among those measurements WHR showed the weakest correlation. There was also a different tendency in the power of correlation between anthropometric dimension with BMI and percentage of BF in males and females. Yet, all the measurements were supposed to be performed to incorporate evaluation on human body composition. Key words: body composition - Javanese adults - anthropometric measurements - percentage of body fat.
oai:jurnal.ugm.ac.id:article/2974
2015-11-18T02:04:53Z
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2015-11-18T02:04:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 02 (2009)
The Comparison of Hydration Effect of 1% Aloe Vera Extract Cream and 10% Urea Cream as Moisturizer for Non Dermatotic Skin
Fajar Waskito, Laily Noor Qomariah, Sunardi Radiono,
2015-11-18 09:04:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2974
id
Background: Xerotic or dry skin is a condition with manifestation of scaly, coarse skin with itchy sensation. Moisturizers have been used as treatment to alleviate xerotic skin. Aloe vera is a traditional medicinal plant which has been used as skin moisturizer for a long time, however, the hydration effect of this material had not been studied. Urea is a humectant material that has been widely used as moisturizer. Objective: This study was aimed to compare the hydration effects of 1 % Aloe vera extract cream with 10% urea cream as moisturizer for non-dermatotic dry skin. Methods: This research was a single-blinded randomized controlled trial. Thirty research subjects who met inclusion criteria were given 2 types of interventions (1 % Aloe vera extract cream or 10% urea cream). Randomization was conducted to determine which cream was applied on the subject's lower arm. Cream was given as a single application. Evaluation of stratum corneum hydration level was conducted objectively using corneometer instrument: before application, 30 minutes, 1 hour, 2 hours, 3 hours and 4 hours after single application. Data of the two groups were analyzed with paired t-test. Results: There was no significant difference of stratum corneum hydration level between 1 % Aloe vera extract cream treatment and 10% urea cream at each time of measurement (p > 0.05). Conclusion: 1 % Aloe vera extract cream showed similar or equivalent hydration effect with 10% urea cream as moisturizer for non-dermatotic dry skin. Key words: aloe vera - urea - skin moisturizer - skin hydration.
oai:jurnal.ugm.ac.id:article/2975
2015-11-18T02:04:53Z
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2015-11-18T02:04:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 02 (2009)
The Efficacy of the Combination of 2% Lidocaine and 0,5% Bupivacaine as Preemptive Analgesia for post-operative pain in vitrectomy with or without scleral buckle under genral anasthesia
Angela Nurini Agni, Marlyna Afifudin, Hartono,
2015-11-18 09:04:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2975
id
Background: Post-operative pain is one of the main problems in the surgical services so that analgesia is very much needed, even intravenously given. The concept of preemptive analgesia in surgical intervention is the provision of analgesia before the occurence of nociceptive stimulus. One of the agents for providing preemptive analgesia is local injection is local injection with local anasthetic, single or in combination. Objective: This study was aimed to evaluate the efficacy of combination of lidocain and bupivacain as preemptive analgesia in vitrectomy with or without sclera buckle under general anesthesia. Methods: Twenty eight patients who were scheduled for vitrectomy were included in this study. The patients were randomly classified into two equal groups. The surgery was conducted under general anesthesia for both groups, but in Group 1, subtenon anesthesia with local anesthetic was given as preemptive analgesia after induction anesthesia and before the start of surgery. Pain intensity was the primary outcome of this study. The pain intensity was measured in 3 hours and 24 hours after surgical procedure. Results: The baseline characteristics between the two groups were similar. The pain intensity was lower in the treatment group, but the difference was not statistically significant (1.00:t 1.71 vs 1.50:t 2.16, p = 0.109). The proportion of pain-free patients were similar between the two groups in 3 hours after procedure (65% vs 65%, p = 1.00). The proportion of pain-free patients were higher in the treatment group in 24 hours after procedure (100% vs 73%, p = 0.222, Fisher's Exact Test}. The subtenon injection procedure was safe. There was insignificant difference in the incidence of side effects between the 2 groups. Conclusion: This study did not show any additional benefit of subtenon injection for vitrectomy. The pain intensity was similar between two groups. There were no increases on side effects after the procedure. Key words: vitrectomy - local anesthetic - post-operative pain - preemptive analgesia
oai:jurnal.ugm.ac.id:article/2976
2015-11-18T02:04:53Z
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2015-11-18T02:04:53Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 02 (2009)
The Relation Between the Expression of latent Membrane Protein-1
Sofia Mubarika H, Soeripto, Murtono C,
2015-11-18 09:04:53
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2976
id
Background: In vitro studies reveal that LMP1 induces the expression of many factors for invasion and metastasis. Objective: The aims of this study were to investigate the role of LMP1 expression on the status of Nand M that take place either directly or sequentially through its relation with the expression IL4, IL6. IL 10. IFN-gamma. Gran2yme B and with protease MMP2. MMP9. either in the serum or in the tumour tissue. Methods: Forty one 41 EBER-RISH confirmed NPC cases with parallel serum were used for this study. Immunohistochemistry was used to study the expression of proteins in the tissue. The serum level of proteins were determined with ELISA. Statistical analysis used Lisrel 8.50. Results: No correlation between the expression of LMP1 with the status of Nand M of the tumour either directly or that sequentially took place through the expression of the cytokines and MMPs either in the tumour tissue or the serum level. The serum level of MMP9 was correlated with the serum level of IL6, the status of N. and the expression of IL 10. Status N was correlated with serum level of MMP2. Conclusion: There is a process that sequentially took place through the increase of the cytokines. MMPs, either in the tumour tissue or serum level and N. M status. LMP1 expression was not involved in the process. Key words: NPC - metastasis - MMP - cytokine
oai:jurnal.ugm.ac.id:article/2977
2016-04-29T07:05:37Z
bik:ART
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2016-04-29T07:05:37Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 01 (2009)
The Change of Retinal Sensitivity in Diabetic Retinopathy after Photocoagulation
Angela Nurini Agni, Arif Budiman, Hartono, Angela Nurini A
2015-11-18 09:05:01
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2977
id
Background: Panretinal photocoagulation is used to destroy new blood vessel growth which develops on large areas of retina. Burns that occur would cause scars, that would stop bleeding. Laser has shown benefit in decreasing advance visual loss. Several studies stated that panretinal photocoagulation also decreased the light sensitivity, peripheral visual acuity, and night visual, paracentral scotoma, choroidal neovascularization (rare). submacular fibrosis, and laser injury cycatrical widening, Study on this problem is needed. Objective: To find out the influence of panretinal photocoagulation on retinal sensitivity in diabetic retinopathy patients. Methods: This study used pretest-posttest design. Subjects were diabetic retinopathy patients who visited Ophthalmology Outpatient Clinic Unit Dr. Sardjito Hospital and Dr. Yap Eye Hospital, Yogyakarta from March 2008 to January 2009. All subjects underwent panretinal laser photocoagulation and visual field was examined before treatment and 1 month (4 weeks) after treatment. Data were analyzed descriptively. The difference of retinal sensitivity before and after treatment were analyzed with paired Hest. Result: 19 patients (34 eyes) were included in the study. Average duration of diabetes mellitus in the subjects was 10.44 + 5.26 years. Most (82.4%) subjects had no history of hypertension. The number of laser given to subjects were 826-1850 burns (the average was 1164+218.95). There was improvement in the retinal sensitivity after photocoagulation, although it was statistically insignificant (p > 0.05). There was no significant difference between old and new parameters on visual acuity improvement (p = 0.72 Conclusion: There was no significant change in retinal sensitivity between before and 4 weeks after panretinal photocoagulation in diabetic retinopathy patients. Key words: retinal sensitivity - panretinal photocoagulation - diabetic retinopathy
oai:jurnal.ugm.ac.id:article/2978
2016-04-29T07:05:45Z
bik:ART
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https://journal.ugm.ac.id/bik/article/view/2978
2016-04-29T07:05:45Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 01 (2009)
Comparison of daily vs. weekly single-dose ferrous sulphate treatment in female junior high students with iron deficiency anemia
Madarina Julia, Dedy Afandi, Sri Mulatsih,
2015-11-18 09:05:01
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2978
id
Background: Complianc~ of daily treatment of iron deficiency anemia in children is still low. The compliance will be increased if the iron supplementation is given weekly. Previous study showed that there was no significant difference in the increase in hemoglobin level between daily and weekly treatment in prepubertal children. Objective: To study the difference in the increase in hemoglobin and serum ferritin levels between daily and weekly sirigle-dose treatment of ferrous sulphate in female junior high school students with iron deficiency anemia after menarche. Methods: This was a quasi-experimental study recruiting 179 and 174 anemic female students in the weekly and daily group, respectively. They receive weekly or daily single-dose of ferrous sulphate capsules. Hemoglobin levels were measured before and after the 12 weeks treatment, while serum ferritin levels were measured before and after in a subset of the study subjects. Result: Mean:t SD levels of hemoglobin before and after iron supplementation were 11.18:1: 0.51 and 12.79:t0.63 g/dl (p=0.001) in the weekly group, and 11.17:1:0.61 and 12.68:1:0.57 g/dl (p=0.001) in the daily group. Mean:l: SD levels of ferritin before and after iron supplementation were 6.95:1: 1.85 and 41.5:1:33.93 ng/ml (p=0.001) in the weekly group, and 6.61 :1:2.17 and 40.7:1:22.73 ng/ml (p=0.001) in the daily group. The prevalence of anemia after supplementation is similar in both groups, i.e. 6.7% in the weekly group and 8.0% in the daily group (p = 0.631. There were no significance difference in the occurrence of side effects of diarrhea and nausea in both groups (p>0.05). Conclusion: This study concluded that daily vs. weekly ferrous sulphate supplementation did not result in significantly different level of both hemoglobin and serum ferritin after treatment. The difference in the occurrence of side effects was also not statistically significant. Key words: anemia iron deficiency - hemoglobin level - serum ferritin level - ferrous sulphate
oai:jurnal.ugm.ac.id:article/2979
2016-04-29T07:05:54Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2979
2016-04-29T07:05:54Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 01 (2009)
Giant fetus in fetu in a female 'child with fever: A case report
Rochadi, Mei Neni Sitaresmi, Soeripto, Hanggoro Tri Rinonce Pieri Kumaladewi, Siti Iqbalwati
2015-11-18 09:05:01
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2979
id
Fetus in fetu is a form of monozygotic diamniotic twin pregnancy where the parasitic twin installs and grows in the body of its partner. It is a rare condition, with a reported incidence of 1 in 500.000 live births. The majority of these cases occur in children younger than 18 months with the chief complaint of an intraabdominal mass, and have a 2:1 male predominance. The size and weight of the fetus vary, from 4 cm to 24.5 cm and from 1.2 g to 1.8 kg. Whether this entity represents a highly differentiated teratoma versus a parasitic twin remains controversial. The objective of this paper was to report a rare condition of giant fetus in fetu in female child with fever, especially to emphasize the importance of proper examination. Although fetus in fetu is very rare, it should remembered as a possibility in an intraabdominal. In some cases further detailed examinations should be done to confirm the diagnosis. A rare case of giant fetus in fetu in a 20th month female patient with fever was reported. Intrabdominal mass was found as the first sign related to fetus in fetu but was neglected by the parents. USG, plain abdominal photo and CT-scan had revealed the diagnosis preoperatively. Proper examination of the fetus in fetu was done including grossly by doing extensive dissection, and detailed histological tissue examination from several sites to make diagnosis completely. Key words: giant fetus in fetu - fetiform teratoma - congenital abnormality - retroperitoneal, fever
oai:jurnal.ugm.ac.id:article/2980
2019-11-12T01:28:45Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2980
2019-11-12T01:28:45Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 01 (2009)
The influence of stigma and depression on quality of life of leprosy patients
Agnes S. Siswati, Jenny MC Siagian, Carla R Marchira
2015-11-18 09:05:01
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2980
id
Background: Stigma attached to leprosy-affected individuals is one of the strongest stigma of diseases. Depression is often occurred to many lepers. Stigma and depression are two factors which affect the quality of life of the lepers. Objective: To determine whether stigma and depression affect the quality of life of leprosy patients in Dermatovenereology outpatient clinic at RSUP Dr Sardjito Hospital. Methods: It was a quantitative and cross-sectional research. The quantitative analysis was conducted with chi-square and multiple regression methods. Result: There was no significant correlation between stigma and quality of life of leprosy patients. The factors which significantly correlated with quality of life were gender, income, and depression score (p < 0.051. Depression score significantly affected the quality of life (B 1.876; P < 0.05; CI 1.166-36.5661. Conclusion: Depression affected the quality of life more than deformity and demographic factors among leprosy patients in Dermatovenereology outpatient clinic at RSUP Dr Sardjito Yogyakarta. Key words: stigma - depression - lepro
oai:jurnal.ugm.ac.id:article/2981
2016-04-29T07:06:11Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2981
2016-04-29T07:06:11Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 01 (2009)
The influence of endometriosis peritoneal fluid on the development of mouse embryo in in vitro pre-implantation
Hasto Wardoyo, Ita Fauziah, O. Wahyu, Djaswadi Dasuki
2015-11-18 09:05:01
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2981
id
Background: Endometriosis was associated with various immunologic changes detected both in the endometrial eutopic and blood edge sample. Some researchers reported that women with endometriosis have an increased amount of peritoneum fluid. Endometrial environment and peritoneal fluid diffusing to the ovarial tube may affect sperm-ovum interaction, initial phase of embryonic development and reduce endometrial receptability. The correlation between endometriosis and early in vitro embryonic development has been reported in some studies showing controversial results; therefore it is necessary to carry out researches to clarify the influence of endometriosis on the early embryonic development. Objective: to identify the influence of peritoneal fluid of infertile women with endometriosis and normal peritoneum fluid of fertile women on the development of mouse embryo in in-vitro pre-implantation. Methods: Prospective, randomized controlled trial subjects are 2 cells of the Swiss albino strain embryos super-ovulating procedure on mouse, 2 embryonic cells resulted from in-vivo fertilization were taken out and in-vitro culture was done with the exposure of 10% or 2.5% cell-free supernatant peritoneum fluid of infertile women with endometriosis and 10% or 2.5% cell-free supernatant peritoneum fluid of normal fertile women as the control. The blastocyst growth was observed in the 72 hour culture and hatching blastocyst culture in 96 hours. Results: An amount of 231 embryos of 2 cells was obtained. After 72 hours, there were 103 blastocyst cultures in both groups. Blastocyst development rate (BDR) culture with to endometriosis peritoneum fluid exposure was lower compared with the normal peritoneum fluid exposure (32.2% vs 56.9%, RR 0.5, 95% CI: 0.42-0.77). Hatching rate (HRI of blastocyst culture with endometriosis peritoneum fluid was lower than that of normal peritoneum fluid (35.1 % vs 92.4%, RR 0.38, 95%CI: 0.24-0.59). The exposure to 10% endometriosis peritoneum fluid lowered BDR significantly compared with the normal peritoneum fluid, 7.7% vs 43.9% (RR 0.18,95% CI: 0.07-0.42); while the exposure to 2.5 % BDR was significantly different, (64% vs 74%, RR 0.87,95% CI: 0.66-1.13). Exposure to 10% compared to 2.5% normal endometriosis peritoneum fluid showed significantly decreased BDR (p 0.05). There was a significant interaction between type of peritoneum fluid (endometriosis vs normal) and the concentration of peritoneum fluid (10% vs 2.5%1 in decreasing BDR (RR 0.17,95% CI: 0.04-0.65). Conclusion: Exposure to endometriosis peritoneum fluid lowered the blastocyst development rate and hatching rate as compared with that to normal peritoneum fluid on the development of the in- vitro mouse embryo model. Key words: embryonic development - peritoneum fluid - endometriosis
oai:jurnal.ugm.ac.id:article/2982
2019-06-17T07:29:19Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2982
2019-06-17T07:29:19Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 01 (2009)
Standardization of Immunocytochemical method for the diagnosis of Dengue Viral Infection in Aedes aegypti Linn Mosquitoes (Diptera Culicidane)
Umniyati, Sitti Rahmah
2015-11-18 09:05:01
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2982
id
methods for virus detection in the mosquito, such as the direct fluorescent-antibody test on head squashes. However, it has the disadvantages of being labor-intensive and requiring fluorescent microscope as well as cryofreezer. Newer methods involving enzyme conjugates such as peroxidase in conjunction with either polyclonal or monoclonal antibodies are greatly improved. With new methods of immunocytochemistry, it is now possible to detect dengue viral antigen in a variety of tissues. Objective: This study was aimed to standardize an immunocytochemical streptavidin-biotin-peroxidasecomplex assay for diagnosis of dengue infection in Aedes aegypti using monoclonal antibody DSSC7. Methods: The infected mosquitoes were held in small cylindrical cages covered with mosquito netting, and incubated at 27:t 1°C ami at relative humidity of 88:t 6 %. The specificity of the immunocytochemical procedure was validated by negative and positive controls showing that the antibody was bound to an appropriate structure. The sensitivity and specificity were also evaluated based on Herrmann's Formula. The presence of dengue antigen on head squash preparation was detected based on ISBPC assay using monoclonal antibody against dengue. The validity and reliability of the measurement were evaluated based on kappa values, according to Landis and Koch. Result: Positive result was detected as discrete brownish granular deposits throughout most visual fields of brain tissue. Dengue viral antigen was immunolocalized to the cytoplasm of brain cells. The immunocytochemical test under light microscope at magnification of 400x was 86.67% sensitive, 96.00% specific, and the kappa value is 0.64. Meanwhile the kappa value between two observers was 0.92, with sensitivity and specificity of 96% and 97% respectively at magnification of 1000x. Conclusion: The monoclonal antibody DSSC7 was sensitive, specific, valid, and reliable as primary antibody to detect dengue viral infection in Ae. aegypti head squash preparation based on immunocytochemical streptavidin-biotin-peroxidase-complex assay under light microscope. Key words: antigen - denguevirus - Aedes aegypti - immunocytochemistry - monoclonal antibody DSSC7
oai:jurnal.ugm.ac.id:article/2983
2016-04-29T07:06:28Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2983
2016-04-29T07:06:28Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 41, No 01 (2009)
The effect of Pandanus conoideus Lam. oil on pancreatic (i-cells and glibenclamide hypoglycemic effect of diabetic Wistar rats
Maliyah Madiyan, Nur Anisah, Winarto
2015-11-18 09:05:01
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2983
id
Background: Oxidative stress has been implicated in pathogenesis and progression of many degenerative disorders including naturally occuring and chemically-induced diabetes mellitus. In addition to the increased production of free radicals, antioxidant defense systems are disturbed in diabetes mellitus. As a result, oxidative stress may cause oxidative damage of cellular, including Ii-cells of pancreas. Objective: This study was aimed at investigating the effect of P. eonoideus Lam. on hypoglycemic activity of glibenclamide and histochemical changes in pancreatic islets. Methods: Thirty male Rattus norvegicus rats were used in the study, and randomly selected. The animals were divided into two groups, each group consisted of 15 rats. Each group was devided into 3 subgroups, the first subgroups were subgroup 1; 2, 3 and the second was subgroup 4, 5, 6. Streptozotocin was injected intraperitoneally at a single dose of 60 mg/kg for induction of diabetes in the second group. Subgroup 1 and 4 did not receive anything, subgroup 2 and 5 received glibenclamide 0.09 g/kgBW/day and subgroup 3 and 6 received P. eonoideus Lam. 0.3 mLlkgBW/day and glibenclamide 0.09 g/kgBW/day. Rats were given P. eonoideus Lam. and glibenclamide everyday for 14 days. Hypoglycemic activity was evaluated in overnight fasted at day 1, 7 and 14, and the acute hypoglycemic activity was evaluated at time 0, 1, 2, 4, and 6 hour aher treatment at day 14. Blood was collected from orbital sinuses. Blood glucose level was determined using GOD-PAP method. At 15 day rat were killed, the pancreas was removed. The data was compared statistically using one-way analysis of variance (AN OVA) followed by post-hoc test and student unpaired t-test. For the analysis of immunohistochemical data, a non parametric test (Kruskal-Wallis) was used. Results: The combination of P. eonoideus Lam. and glibenclamide produced significant reduction in blood glucose in the serial study at day 1, 7 and 14 and comparable with that of standard drug, glibenclamide also control groups in normal and diabetic rats. It also produced significant reduction of blood glucose in the serial study at time 0, 1, 2, 4, and 6 hour after treatment at day 14. Significant increasing histoscore, number and diameter of Langerhans islets were observed in P. eonoideus Lam. treated rats. Conclusion: P. eonoideus Lam. could increase hypoglycemic effect of glibenclamide and histoscore, number, and diameter of Langerhans islets in diabetic rats. Key words: minyak buah merah - pancreas B-cells - glibenclamide hypoglycemic effect - diabetes
oai:jurnal.ugm.ac.id:article/2990
2015-11-18T02:05:17Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2990
2015-11-18T02:05:17Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 04 (2008)
Human papillomavirus (HPV) as the causal factor of tympanal cholesteatoma in malignant-type chronic suppurative otitis media
Rianto, Bambang Udji Djoko
2015-11-18 09:05:17
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2990
id
Background: Tympanal cholesteatoma is an uncoordinated, uncontrolled hyperproliferative epidermic epithelial cells keratinized with aggressive, erosive and destructive development to the mucous membrane and the bone of external auditory canal and tympanic cavity. This process is invasive and may migrate to the surrounding tissue. Ethiopathogenesis of tympanic cholesteatoma is unknown. Histological examination shows that there are hyperproliferative development, papillomatous, koilocyte clusters and destruction of the bone. These abnormalities are characteristics of papillomavirus.Objective: To know the whether HPV-18 is the risk factor in the occurrence of tympanic cholesteatoma ini malignant-type chronic suupurative otitis media.Methods: A case-control study was conducted on 31 tympanal cholesteatoma specimens from malignanttype chronic suppurative otitis media as the case group, and 31 mucosal tympanic cavity specimens from benign-type chronic suppurative otitis media as the control group. For detecting Polymerase chain reaction (PCR) examination was used to detect DNA HPV-18, that performed in Parasitology laboratorium Faculty of Medicine, Gadjah Mada University.Results: Polymerase chain reaction (PCR) examination showed that there were 21 (67.7%) DNA HPV-18 positive specimens in the case group, while in the control group there were 5 (16.1 %) DNA HPV-18 positive specimens. This difference was statistically significant (p = 0.0001; Odds ratio: 1 0.92; 95 % confidence interval = 2.83-45.29). Immunohistochemistry examination was used to identify host immune response to HPV, by observing the interleukin-1 IIl-1) and interferon g IIFN g) expressions. Both Il-1 and IFN g cytokines were highly expressed in tympanic cholesteatoma samples, compared to both in the control group. The difference was statistically significant (p < 0.05, Odds ratio 14.29; 95% confidence interval: 3.64-60.5 and p< 0.05, Odds ratio:10.2; 95% confidence interval: 2.74-40.35, respectively). Conclusion: It can be concluded that HPV-18 DNA was iidentified, and was one of the multiple risk factors in the occurence of tympanic cholesteatoma in malignant-type chronic suppurative otitis media..Key words: tympanal cholesteatoma - human papillomavirus - malignant-type chronic suppurative otitis media
oai:jurnal.ugm.ac.id:article/2991
2015-11-18T02:05:17Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2991
2015-11-18T02:05:17Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 04 (2008)
On-admission serum soluble CD40 ligand level predicts subsequent myocardial damage in acute myocardial infarction
Putrika Prastuti Ratna Gharini, Budi Yuli Setianto Anggoro Budi Hartopo
2015-11-18 09:05:17
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2991
id
Background: Platelet activation plays an important role in plaque rupture and thrombus formation. Activated platelets release soluble CD40 ligand (sCD40LI. It has coagulation and inflammation properties. Enhanced sCD40L activity has been found in acute myocardial infarction, especially in ST-elevation myocardial infarction. The impact of this enhancement on the degree of myocardial damage remains uncertain, although evidence suggests association between sCD40L level and high burden coronary thrombus.Objective: The aim of this study was to find out whether serum sCD40L level on-admission is predictive of subsequent myocardial damage following acute myocardial infarction.Methods: This study was a cross-sectional study. We consecutively enrolled patients with acute myocardial infarction admitted to ICCU Dr. Sardjito Hospital with onset < 24 hours. Blood collection for analysis of sCD40L was withdrawn on-admission before revascularization and heparinization. Serial measurement of creatine kinase (CK-MBllevel in first 24 hours of admission was conducted, the highest level was considered a peak level that reflected myocardial damage. Troponin I was measured in single sample during 72 hours of admission. The association between serum sCD40L and peak CK-MB level was analyzed with Pearson correlation test. Statistical significancy was determined by p value < 0.05.Result: We enrolled 54 consecutive subjects, among them 37 subjects was STEM!. There was a significantly positive correlation between on-admission sCD40L and peak CK-MB level (r = 0.320 ; p value = 0.0201. Nonetheless, sCD40L did not correlate with troponin I level. In STEM I, this correlation was stronger (r = 0.424; p value = 0.0101 and even more stronger in patients with STEMI who underwent revascularization therapy (r = 0.515 ; p value = 0.0291.Conclusion: On-admission serum sCD40L level is predictive of subsequent myocardial damage following acute myocardial infarctionKey words: soluble CD40 ligand - platelet activation - acute myocardial infarction - peak CK-MB myocardial damage
oai:jurnal.ugm.ac.id:article/2992
2015-11-18T02:05:17Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2992
2015-11-18T02:05:17Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 04 (2008)
Diabetes mellitus type 1 in congenital rubella syndrome: a case report
E.S. Herini, Carina Lisa Madarina Julia
2015-11-18 09:05:17
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2992
id
Corina Lisa, Madarina Julia, E.S. Herini . Diabetes Mellitus Type 7 in Congenital Rubella SyndromeDiabetes mellitus type 1 has been reported as a very rare delayed manifestation of congenital rubella syndrome. We reported a confirmed case of congenital rubella syndrome that developed diabetes mellitus at the age of seven years. The girl had been complaining of diabetes symptoms since six months before admission, but had been misdiagnosed. The patient finally came with symptoms of diabetic ketoacidosis, a life threatening complication of diabetes mellitus. Diabetes mellitus type 1 as a delayed manifestation of congenital rubella syndrome has to be kept in mind to prevent missing the diagnosis.Key words: rubella infection - congenital rubella syndrome - diabetes mellitus type 1ABSTRAKCorina Lisa, Madarina Julia, E.S. Herini - Diabetes Mellitus Tipe 7 pada Sindroma Rubella KongenitalDiabetes melitus adalah salah satu manifestasi lambat sindrom rubella kongenital yang jarang, tetapi perlu diwaspadai. Kami melaporkan satu kasus sindrom rubela kongenital yang mengalami diabetes melitus tipe 1 pad a usia 7 tahun. Meskipun gejala diabetes telah dikeluhkan sejak enam bulan sebelum masuk rumah sakit, penyakit diabetes tetap tidak terdiagnosis. Penderita akhirnya datang dengan gejala dan tanda ketosidosis diabetikum, suatu komplikasi diabetes mellitus yang mengancam jiwa. Manifestasi lambat sindrom rubella yang berupa diabetes mellitus tipe I harus diwaspadai untuk mencegah tidak terdiagnosanya penyakit tersebut.
oai:jurnal.ugm.ac.id:article/2993
2015-11-18T02:05:17Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2993
2015-11-18T02:05:17Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 04 (2008)
The effectiveness of rectal and oral paracetamol in decreasing fever in children
Sasmito Nugroho, Gama AF Isnaeni Djauhar Ismail
2015-11-18 09:05:17
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2993
id
Background: The common treatment to reduce fever is administration of paracetamol orally. However, oral route is sometimes not possible due to some problems, such as vomiting, irritability, unconsciousness, and convulsion and therefore, paracetamol should be administered rectally.Objectives: To know the effectiveness of rectal compared to oral administration of paracetamol in febrile children.Methods: This study was a parallel randomized, double-blind, controlled trial, comparing the effectiveness of rectal paracetamol to oral paracetamol. The study subjects were children with fever. Consecutive sampling was used to collect samples, and block randomization was used for random allocation. The study was conducted from November 1, 2006 to April 30, 2008. The body temperature was measured just before taking the drug (to), and was monitored after 30 minutes (t1), 60 minutes (t2), 90 minutes (t3), 120 minutes (t4), 150 minutes (t5), and 180 minutes (t6). Inferential statistic tests used were chi square, t-test and Mann Whitney U test.Results: A total of 176 subjects eligible for the study were randomly assigned to receive either paracetamol 12.5 mg/kgBW rectally (88 subjects) or paracetamol 12.5 mg/kgBW orally (88 subjects). There were 6 subjects who were drop-out and 1 subject failed from rectal group, and 6 subjects were drop-out and 2 subjects failed from oral group. Paracetamol average dose for each group was 12.5 mg/kgBW. The proportion of the decrease in body temperature to 38°C in the first 30 minutes was significantly higher ??? in oral group. The change in body temperature from initial body temperature between oral and rectal routes after 30 minutes was significantly different (to-t1: p=0.003), but later the decrease was notstatistically different (to-t2: p = 0.471, to-t3: p = 0.488, to-t4: p =0.824, to-t5: p = 0.790, to-t6: p = 0.493): the differences between two periods of measurement were not significant (t1-t2: p=0.073, t2t3: p=0.791, t3-t4: p=0,171, t4-t5: p=0.199, t5-t6: p=0.426), except from to-t1 (p=0.005).Conclusion: Rectal paracetamol 12.5 mg/kgBW had a higher effectiveness compared with oral paracetamol to reduce fever in children.Key words: fever - rectal paracetamol - oral paracetamol - effectiveness
oai:jurnal.ugm.ac.id:article/2994
2015-11-18T02:05:17Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2994
2015-11-18T02:05:17Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 04 (2008)
The effect of chronic ethanol administration on total number of pyramidal cells in CA 1 and CA3 of hippocampus and spatial working memory in adolescent rats (Rattus norvegicus)
Mustofa, Muh. Ihwan Nurwanto Soejono Aswin
2015-11-18 09:05:17
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2994
id
Background: Adolescent brain may be more susceptible to damage by ethanol. Chronic ethanol administration may cause morphological changes in the hippocampus, followed by deficit in the hippocampal function, including the impairment of memory.Objective: The aim of this study were to investigate the changes in the total number of pyramidal cells in CA 1 and CA3 of hippocampus, and the relationship between these changes with spatial working memory of rats after chronic ethanol administration at adolescent age.Methods: Twenty five adolescent (30 days of agel male rats (Rattus norvegicusl were classified randomly into 5 groups, that is, namely control without treatment (C1 I, control with treatment (C21, treatment 1(11 I, treatment 2 (T2), and treatment 3 (T31 groups. Each group consisted of 5 rats. C2 group was given physiological saline, while 11, T2, and T3 groups were given ethanol at various doses of 1, 2, and 3 gl kgBW/day, repectively, for 30 days by intraperitoneal injection. After chronic ethanol administration, the spatial working memory of 5 groups were tested using 8 arms radial maze for 12 days. At the end of the experiment, the rats were sacrified and the brains were taken out for histological examination, by paraffin method and Cresyl violet staining.Result: Chronic ethanol administration on adolescent rats caused a decrease in spatial working memory (especially in group T31 (p < 0.051. The total number of pyramidal cells in CA 1 of group T3 was fewer than those of control groups (p < 0.05), but the total numbers of pyramidal cells in CA3 of treatment groups were not different with those of control groups (p > 0.051. Analysis with product moment correlation test showed that there was a positive correlation between the total number of pyramidal cells in CA 1 of hippocampus with spatial working memory of the rats after chronic ethanol administration at adolescent age (p < 0.051.Conclusion: Chronic ethanol administration on adolescent rats caused a decrease in the spatial working memory and the number of pyramidal cells in CA 1 of hippocampus. There was a positive correlation between the number of pyramidal cells in CA 1 and spatial working memory of the rats after chronic ethanol administration at adolescent age.Key words: ethanol - pyramidal cells - spatial working memory - adolescent
oai:jurnal.ugm.ac.id:article/2995
2015-11-18T02:05:17Z
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v2
https://journal.ugm.ac.id/bik/article/view/2995
2015-11-18T02:05:17Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 04 (2008)
Age-Related Variation On Somatotypes Of Javanese People In Y Ogyakarta Province
Kumi Ashizawa, Nani Trilusiana Rahmawati Janatin Astuti
2015-11-18 09:05:17
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2995
id
Background: Body form has been related to a variety of behavioral, occupational, health, and physical performance variables, and somatotype is an effective technique for the study of anthropometric variations, which possess constantly recognizable characteristics and are the functional end products of the whole genetic and the developmental complex.Objective: The objective of this cross-sectional study was to describe age-related variations of somatotype in a cross sectional sample of healthy adult and elderly subjects from Yogyakarta Province Indonesia, and to promote the use of technique for the study of somatotype variations in aged populations.Methods: The study groups, consisted of 426 healthy Javanese people (185 males and 241 females) of age 18 - 90 years. The men and women samples were further divided into four groups: 18 - 20 years, 21 - 40 years, 41 - 60 years, and 61 - 90 years. All subjects were born and resident in Yogyakarta Province. Heath-Carter somatotypes were determined for all subjects.Result: The height and weight are significantly greater in males than in females. There was the relationship between age and somatotype component. The mean somatotype of the overall Javanese sample (18-90 years) is 3.0 - 3.2 - 2.9 or center type (SO: 1.2 - 1.3 - 1.3) in males and 4.4 - 3.3 - 2.2 or mesoendomorphic type (SO: 1.2 - 1.5 - 1.3) in females.Conclusion: In general, endomorphy and ectomorphy showed a week and even negative correlation with age group, while mesomorphy show a low but positive correlation. Significant sex differences in somatotype appeared only in endomorphy where females were higher than males. Javanese males somatotypes were less endomorphic and ectomorphic, but more ectomorphic than Javanese females.Key words: somatotype component - body size - variation - Javanese people
oai:jurnal.ugm.ac.id:article/2996
2015-11-18T02:05:17Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2996
2015-11-18T02:05:17Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 04 (2008)
Effect of homocysteine and antioxidants on peroxidation lipid of essential hypertension in Central Java Indonesia
Rizka H Asdie, Mohammad Hakimi, Sunarti Ria Maudisa
2015-11-18 09:05:17
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2996
id
Background: The increase in the homocysteine level is related to the heightening of blood pressure and hypertension risk. Hyperhomocysteinemia encourages the production of free radical which can oxidi2e lipid. Lipid peroxidation can be prevented by several antioxidant such as vitamin C and E.Objective: This study is aimed at finding out the nature of the interaction effect between homocysteine and antioxidant vitamins on malondialdehyde level in essential hypertension.Method: This study was a case-control nested to NSurveiliance of Non-Communicable DiseasesN in Purworejo, Central Java. The subjects were men, essential hypertension and 20-60 years old that have normal value of urine creatinine, blood glucose, cholesterol and triacylglycerol. They have been take antihypertension drugs. The blood glucose, cholesterol, triacylglycerol, urine creatinine, vitamin C and malondialdehyde (MDA) levels were measured by spectrophotometer. Whereas the total plasma homocysteine and vitamin E were determined by HPLC.Result: The homocysteine level in the cases was higher than that of the control and was significantly different (P = 0,027). There were not significantly different of the vitamin C, vitamin E, and MDA between the cases and the control (P=0.538, P=0.390, and P=0.536) respectively. The MDA was positively correlated with homocysteine (P=O.OOOI and negatively correlated with vitamin E (P=0.029), but it was not negatively significant correlated with vitamin C (P=0.0671. The correlation had r= 0.417; R2=0.174 and F=7.713.Conclusion: The high homocysteine level is one of hypertension risk factors and its effect through oxidative stress. The effect can be prevented by antioxidant vitamins, specially vitamin E.Key words: homocysteine - antioxidants - peroxidation lipid - essential hypertension - avanese
oai:jurnal.ugm.ac.id:article/2997
2015-11-18T02:05:17Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2997
2015-11-18T02:05:17Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 04 (2008)
Correlation of interleukin-6 and monocyte count to troponin I in acute coronary syndrome
Usi Sukorini, Teguh Triyono Joni Parinding
2015-11-18 09:05:17
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2997
id
Background: Acute coronary syndrome (ACS) becomes an important disease, because of its increased prevalence and mortality rates. Makers of systemic inflammation, including interleukin-6 and monocyte count, have been shown to predict future cardiovascular events. However, the association between these inflammatory markers with cardiac injury is still a controversy.Objective: To evaluate the relationship between IL-6 and monocyte count as inflammation markers and troponin I as cardiac injury.Methods: This was a cross-sectional study. Subjects were patients suspected of ACS admitted to the emergency department of Sardjito Hospital Yogyakarta. Diagnoses were performed based on WHO criteria. Troponin I and interleukin-6 levels were measured from sera using sandwich ELISA method (Roche). Monocyte counts were determined by automatic hematology analyzer (Sysmex). Kruskal-Wallis test and Spearman's correlation test were used to assess the difference and correlation between parameters.Results: Sixty one (61) subjects were included in the study. Age of the subjects were 51-60 years, and diagnosed as ST elevation myocardial infarct (STEMI). Significant differences of IL-6 and troponin I levels were determined among unstable angina pectoris (UAP), non-ST elevation myocardial infarct (NSTEMI), and ST elevation myocardial infarct (STEMI) subjects. There was a moderate correlation (r = 0.54; p = 0.001) between troponin I and Interleukin-6, but not with monocyte count.Conclusion: Interleukin-6, but not monocyte count, was correlated with troponin I in acute coronary syndromes.Key words: acute coronary syndrome - interleukin-6 - monocyte count - troponin I
oai:jurnal.ugm.ac.id:article/2998
2015-11-18T02:05:43Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2998
2015-11-18T02:05:43Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 03 (2008)
Low-dose short-course metformin decrease the risk of atherosclerosis in the non-obese prediabetic individuals
Djoko Hardiman, Djoko Hardiman
2015-11-18 09:05:43
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2998
id
Background: The establishment of macrovascular (cardiovascular) event is initiated in the period of prediabetic. Metformin plays role in the carbohydrate and lipid metabolism as well as vascular protection. The mechanism of the cardiovascular event risk increased in the pre-diabetic individual has not been clearly established.Objective: The study was to determine if insulin resistance control by low-dose short-course metformin administration in the non-obese prediabetic individual might decrease the risk of atherosclerosis.Methods: It was a prospective experimental double blind study with pretest-posttest control group design. It was conducted on the first degree relatives of type-2 Diabetes Mellitus patients who met the criteria of non-obese prediabetic individuals. Subjects were randomly classified into treatment group (designed for metformin administration), and the control group (designed for placebo administration). The prediction of atherosclerosis risk was based on the hsCRP, PAI-1, VCAM-1, and fibrinogen levels.Result: The results showed that the margin between pre- and post-metformin administration term were 1.89 vs. 0.06 mg/L (p=0.001), 1.42 vs. 0.84 IU/mL (p=0.0151. 180.85 vs. 03.81 mg/L (p=0.061) and 80.15 vs. 31.42 mg/dL (p=0.001) for hsCRP, PAI-1, VCAM-1, and Fibrinogen levels in the treatment and control group, respectively.Conclusion: The study concluded that the low-dose short-course metformin administration might decrease the atherosclerosis risk significantly in the non-obese prediabetic individuals.Key words: metformin - non-obese prediabetics - hsCRP - PAI-T- Fibrinogen - VCAM-T
oai:jurnal.ugm.ac.id:article/2999
2015-11-18T02:05:43Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/2999
2015-11-18T02:05:43Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 03 (2008)
Prevalence and risk factors of hand dermatoses among pandan handicrafters
Fajar Waskito, Erni Setiyawati Devi Artami S, Sri AwaliaFebriana,
2015-11-18 09:05:43
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/2999
id
Background: Pandan handicraft is one of the industrial sectors which has a significant role in employing people in Kulonprogo, Yogyakarta. Development in this industry makes higher material exposure to the hands during the production procedures, that makes it essential to study the risk of suffering from hand dermatoses among the handicrafters.Objective: To determine the prevalence and risk factors of hand dermatoses among pandan handicrafters. Methods: Cross-sectional study was conducted on 80 subjects who was taken randomly from Pandan handicrafter population in Tanjungharjo village, Kulonprogo, Yogyakarta. Prevalence data were analyzed descriptively. Risk factors were analyzed using estimation of prevalence ratio (pr), 95% Confident Interval (el), and chi-square test.Results: Out of 74 subjects fulfilled the criteria, 58 (78.4%) were suffered from hand dermatoses. Callus was the most common disease occurred in 49 (66.2%) subjects, followed by irritant contact dermatitis (lCD) in 18 (24.3%) subjects. History of atopy was proven as risk factor for ICD (p = 0.025, pr = 2.872; 95%el = 0.913-9.032). Types and duration of work were not proven as risk factors, as well as no gloves protection during work.Conclusion: Callus was the most common hand dermatosis occurred among pandan handicrafters, followed by ICD. History of atopy was identified as a risk factor for ICD.Key words : pandan - handicrafter - hand dermatosis - occupational-atopy
oai:jurnal.ugm.ac.id:article/3000
2015-11-18T02:05:43Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3000
2015-11-18T02:05:43Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 03 (2008)
The use of bacteriophage therapy for curing the Escherichia coli 0157 infection in mice
Metha Restu, Rio Rendy, Hera Nirwati, Susi Iravati, Mova Aria, Ida Ayu Putu
2015-11-18 09:05:43
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3000
id
Background: Diarrhea is still a problem in public health, especially in developing country such as Indonesia. Escherichia coli 0157:H7 {Enterohemorrhagic Escherichia coli (EHEC)} is one of the important strains as the etiology of bloody diarrhea with sistemic complications such as hemolytic uremic syndrome and hemorrhagic colitis. The increase in the discovery of E. coli 0157:H7 resistance to antibiotic is a worldwide problem that must be solved. Bacteriophage application can be a promising alternative therapy. In addition, bacteriophage can also be used as diagnostic tool for bacterial identification and as biocontrol agent in bacterial water pollution.ObJective: The aim of this study was to isolate and identify a specific bacteriophage using a specific strain of E. coli 0157 and use this bacteriophage to cure the E. coli 0157 infection in mice.Methods: E. coli was isolated and identified from faecal samples of diarrheal patients from many Primary Health Centers in Yogyakarta, using McConkey Agar and biochemical media. E. coli 0157 was determined using sorbitol McConkey Agar and agglutination test. Toxins of these strains were detected using hemolysis assay method. Bacteriophage was isolated using one of E. coli 0157 strain (E. coli K-151) from river water of Kali Mambu. The therapeutic effect of this bacteriophage was studied using eighteen threemonth-old male mice of Swiss strain. They were classified randomly into three groups (6 groups). Mice in group A and B were infected with 0.5 ml of 108 CFU of E. coli suspension orally. Only mice in group A were treated with bacteriophage 1.10"pfu/mL, while those in group B were not treated, and group C was used as control.Results: Three strains of E. coli 0157 (K-151, K-840 and K-854) were isolated among 70 E. coli isolates. Bacteriophage K-151 was isolated from the river water. Average cure duration in the group who was given phage K-151 therapy was 34.17 hours, and average cure duration in the group that was not given phage K-151 therapy was 72.7 hours. The mortality rate of group A was 0%, while in group B was 17%. Conclusion: Bacteriophage is effective as alternative therapy against E. coli infection in mice.Keywords: E. coli 0157 - bacteriophage - hemolysis assay - diarrhea
oai:jurnal.ugm.ac.id:article/3001
2015-11-18T02:05:43Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3001
2015-11-18T02:05:43Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 03 (2008)
The different expressions of urokinasetype plasminogen activator, urokinasetype plasminogen activator receptor, and plasminogen activator inhibitor-1. Several scores of cell pleomorphism, number of mitosis and keratiniza
Totok Utoro, Irianiwati, Ahmad Ghozali
2015-11-18 09:05:43
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3001
id
Background: Urokinase plasminogen activator (uPA) system plays important role in cancer invasion and metastasis. The real function of PAI-1 is to protect extracellular matrix from degradation. But, there are still arguments about the function of PAI-1. In colorectal cancer, breast cancer, and liver cancer, high expressions of uPA and PAI-1 are related to poor prognosis of the patients. In squamous cell carcinoma of the cervix, the role of uPA system in its relation with histological grading (consists of several scores of cell pleomorphism, number of mitosis and keratinization index) are still unclear.Aim: To find out the different expressions of uPA, uPAR, PAI-', and ratio of uPA!PAI-1 among several scores of cell pleomorphism, number of mitosis and keratinization index of cervical cancerMethods: Thirty five paraffin blocks of squamous cell carcinoma of the cervix were grouped histologically based on the score of cell pleomorfism, number of mitosis, and keratinization index. The specimens were stained immunohistochemically with MoAb against uPA, uPAR, and PAI-1 with chromogen DAB and counterstain Meyer Hematoxyllin. The expression of uPA, uPAR, PAI-1, and ratio of uPA/PAI-1 were counted based on the number of positive expression tumor cells!100 tumor cells in five microscopic fields. The different expression of each variable will be analyzed with One Way Anova analysis.Result: There were statistically insignificant differences between the expressions of uPA, uPAR, PAI-1, and ratio of uPA/PAI-' based on the score of cell pleomorfism (p = 0.463, 0.731, 0.541, 0.460, respectively), keratinization index (p = 0.207, 0.062, 0.231, 0.576, respectively), and nlimber of mitosis (p = 0.359, 0.125, 0.428, 0.340, respectively) of squamous cell carcinoma of the cervix.Conclusion: The result suggested that the expressions of uPA, uPAR, PAI-1, and ratio of uPA/PAI-1 did not have important roles in cells pleomorfism, number of mitosis, and keratinization index of cervical cancer.Key words: uPA - uPAR - PAI-' - ratio of uPA!PAI-1 - cell pleomorphism - number of mitosis - keratinization index - cervical cancer
oai:jurnal.ugm.ac.id:article/3002
2015-11-18T02:05:43Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3002
2015-11-18T02:05:43Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 03 (2008)
The effect of mahkota dewa (Phaleria macrocarpa (Scheff) Boerl) leaf etanolic extract on splenic NK 1.1 cells activity
Sofia Mubarika Haryana , Sismindari, Muhammad Ghufron Marsetyawan Soesatyo
2015-11-18 09:05:43
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3002
id
Background : Mahkota dewa (Pheleria macrocarpa (Scheff, Boerl) is an herbal medicine which has been used since many years as traditional medicine in Indonesia against infections.Objective: The study was aimed to know the effect of mahkota dewa extract on cellular immune response, in particular NK1.1 cell activities.Methods: Forty C57BL/6 mice used in this study were divided into 8 groups. In this study, different doses of etanolic extract of mahkota dewa leaf were used i.e. 1.05 mg, 2.1 mg, and 4.2 mg/20g BW. Groups I, II, and III were treated with 1 mg, 2.1 mg, and 4.2 mg/20 g BW of the extract daily for 30 days. In group IV, V, and VI the extract was given simultaneously during 30 days, then the mice were infected with 104 cfu of Listeria monocytogenes (A TCC-191151 for stimulating their immune responses. Whereas group VII was untreated control group, and group VIII received only Listeria monocytogenes. All mice were then sacrificed 48 hours after the last treatment. Splenocyte NK1.1 cells were collected then cultured with YAC-1 (ATCC:TIB 1601 target cells for killing activity assay, expressing NKG2D, CD122 and IFNy assay. Results: The results showed that an oral administration of the extract significantly increased the killing activity of splenic NK 1.1 cell against the target, Y AC-1 cell. Moreover, the extract promoted the secretion of IFN-y from NK1.1 cells, and also induced expression of both surface molecule NKG2D and CD 122. The strongest effect stimulation was on the dosis of 2.1 mg/20 g BW.Conclusion: The extract had effect to augment splenic NK1.1 cell activities, as indicated by increasing their killing activity, expression of surface molecules and IFN-y production.Key words: pha/eria macrocarpa - splenic NK1.1 cell activity - YAC-1 cell - IFNy - NKG2D and CD122 surface molecules
oai:jurnal.ugm.ac.id:article/3003
2015-11-18T02:05:43Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3003
2015-11-18T02:05:43Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 03 (2008)
Premenstrual syndrome (PMS) and premenstrual dysphoric disorder (PMDD) in Indonesian women
Ova Emilia, Ova Emilia Ova Emilia
2015-11-18 09:05:43
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3003
id
Background: Data from epidemiologic surveys showed that the burden of illness caused by premenstrual syndrome could not be underestimated. It has been estimated that the frequency of premenstrual symptoms was quite high (80-90%1, and in some women, the symptoms were so severe that they might interfere with daily activities. Most epidemiological studies of PMS were conducted in Western women, though a few small sample-size studies of Chinese and Japanese women indicated that they were affected by PMS. Objective: To find out the characteristic features of premenstrual symptoms in Indonesian women.Methods: Four hundred and sixty women aged between 20 and 49 yrs. with regular menstrual cycles, who were seen at a Women Health Clinic, were assessed regarding their premenstrual symptoms using the Premenstrual Symptoms Screening Tool (PSSTI. They were able to provide informed consent. The study was conducted in July 2007 until January 2008.Results: As many as 95% of these women were found to have at least premenstrual symptoms. The rates of prevalence of moderate to severe PMS and PMDD in this study were 3.9% and 1.1 %, respectively, which were lower than those in Western or Chinese and Japanese women. Only 5 women with moderate to severe PMS and PMDD were treated and 10.5% women seek herbal medicine to reduce premenstrual symptoms.Conclusion: Severe to moderate premenstrual symptoms and PMDD were suffered by 5% women and mostly affected 20-29 year old women. Medical treatment was rarely sought, but herbal medicine was often used to relieve premenstrual symptoms.Key words: PMS - PMDD - Indonesian women
oai:jurnal.ugm.ac.id:article/3004
2015-11-18T02:05:43Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3004
2015-11-18T02:05:43Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 03 (2008)
Hantavirus infection in clinically suspected dengue fever patients
Hera Nirwati, Praseno
2015-11-18 09:05:43
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3004
id
Background: Hantavirus has been found in many parts of the world, including newly isolated virus in Indonesia. Although infection with the virus can result in specific signs and symptoms known as hemorrhagic fever with renal syndrome (HFRS), clinical presentation of the disease may be similar to those of dengue or other viruses infection. Diagnosis of both dengue and hantavirus infection should be established by laboratory test for the detection of specific antibodies.Objective: The aim of this study was to determine the presence of hantavirus infection in patients suspected to have dengue fever.Methods: Sera were prepared from venous blood of patients. Specific IgG and IgM anti hantavirus in sera from clinically suspected dengue fever patients were examined by an indirect immunoflourescence antibody technique.Results: Eight percent of sera samples were positive for both specific IgG and IgM anti hantavirus, whereas 1 2 percent of samples were positive for IgG only.Conclusion: It is concluded that the recent infection with hantavirus have been found in 8 percent of clinically suspected dengue fever patients, whereas 12 percent of the patients were infected with the virus some time in the past.Key words: hantavirus - immunofluorescence antibody technique - dengue fever - specific IgG and IgM anti hantavirus
oai:jurnal.ugm.ac.id:article/3005
2015-11-18T02:05:43Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3005
2015-11-18T02:05:43Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 03 (2008)
The effect of fitness dance for elderly on stature/arm span ratio of non-trained old women in Y ogyakarta
M. Mansyur Romi, Santosa Budiharjo Djoko Prakosa
2015-11-18 09:05:43
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3005
id
Background: Aging process causes changes of the body composition, body posture, and physical performance. In elderly people, the decrease in muscle mass, muscle strength, and flexibility of body segments reduce the maximum height at the upright standing position.Objectives: This study was aimed to find out the effects of fitness dance for elderly (FDE) on stature/arm span ratio of non-trained old women in Yogyakarta.Methods: Pretest and posttest control design was used. The samples consisted of 30 non-trained healthy old women of Yogyakarta, aged 60-70 years. They were classified into three groups, each consisted of 10 women, namely: Group I (GI) was given FDE 3 days a week. Group II (Gill was given FDE 2 days a week, and non-treatment control group (CG). FDE is a low impact aerobic exercise, created by Association of Indonesian Sports Women, Yogyakarta Branch. The FDE in this research was'the D series (low impact aerobic). The treatment was performed for 8 weeks, 40-54 minutes in each session. The intensity of exercise was maintained at 70-79% of maximal heart rate by controlling the musical beat. Before and after treatment (in the 2nd, 4th, 6th, 8th week), the subjects were measured using the stature and arm length anthropometry. This study was conducted in April-June 2003 at Kotagede, Yogyakarta.Results: The one way variance analysis among groups revealed that the ratio of stature/arm span was significantly improved (p
oai:jurnal.ugm.ac.id:article/3006
2015-11-18T02:05:51Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3006
2015-11-18T02:05:51Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 02 (2008)
The combination effect of triamcinolone acetonide and tamoxifen citrate on fibroblast populated collagen lattice contractions
Pranoto, Agung
Pudjiati, Satiti Retno
Wirohadidjojo, Yohanes Widodo
2015-11-18 09:05:51
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3006
id
Background: Keloid is caused by fibroblast hyperproliferation stimulated by transforming growth factor-IH ITGF-131 I, and it is usually treated with triamcinolone acetonide (TAl, which has the ability to inhibit TGF131 synthesis. However, the clinical results is still unsatisfied. Another drug that may inhibit keloid fibroblast TGF-131 synthesis is tamoxifen citrate (TCI, but the effect of the combination on keloid fibroblast activities has never been published.Objective: To find out the effect of combined triamcinolone acetonide and tamoxifen citrate on fibroblast keloid activities in vitro.Methods: It was a parallel post-test only study. The third passage keloid fibroblasts were isolated from a patient with keloid, cultivated in collagen lattice, and treated with several combinations of 5, 10, and 20 pM TA and 10, and 20 pM TC. Lattice contractions were measured based on digital image using scion image.Results: Among TA groups, the best inhibition of lattice contraction was found among 20 pM treated group and among TC groups. The best inhibition of lattice contraction was found among 20 pM TC. The best combination was found in the combination of 20 pM TA plus 20 pM TC.Conclusion: The result indicated that a combination of triamcinolone acetonide and tamoxifen citrate had a significant role in suppressing fibroblast activity, better than triamcinolone acetonid or tamoxifen citrate alone.Key words: tamoxifen - triamcinolone - collagen lattice - keloid fibroblast.
oai:jurnal.ugm.ac.id:article/3007
2015-11-18T02:05:51Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3007
2015-11-18T02:05:51Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 02 (2008)
The Effecct of cisplatin on male white mice testicular tissue
Dick Moch. Rizal, Dick Moch. Rizal
2015-11-18 09:05:51
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3007
id
Background: Cisplatin is a potent anticancer drug. In the therapy of various kinds of tumours. such as testicular, ovarian, bladder and lung cancer, cisplatin is used. It has cytotoxic and antimitotic effect on cell and tissue. Some studies reported the negative effect of cisplatin on male reproductive system.Objective: To know the effect of cisplatin on testicular tissue of male white miceMethods: Ten male white mice of DDI strain, 2 months old, 28.8-33.0 grams were classified into control group (5 mice) and treatment group (5 mice). The animal models were given standard feeding LPPOM, while those the control group were given CMC-Na 0.5% orally for 7 days. Cisplatin was given to the treatment group intravenously in the dose of 3 mg/kgBW for 7 days. At the end of the experiment, the animal model was killed by decapitation and the testicle was taken out. The testicle was placed in 10% formalin solution and processed for histology preparation with hematoxilin (Meyer) eosinand analyzed under binocular microscope.Results: All mice in the treatment group showed mild to severely damaged testis. There were serious spermatogenic and Sertoli cell disruption in severely damage testis.Conclusion: Intravenous injection of 3 mg/kgBW cisplatin for 7 days had deleterious effects on testicular tissue of male white mice, resulting in mild to severe damage.Key words: cisplatin - spermatogenesis - tubulus seminiferous - testicular damage
oai:jurnal.ugm.ac.id:article/3008
2015-11-18T02:05:51Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3008
2015-11-18T02:05:51Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 02 (2008)
Evaluation of substrate metabolism in critically ill pediatric patients
Antonius H Pudjiadi, Nurnaningsih
2015-11-18 09:05:51
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3008
id
Background: Critically ill pediatric patients are characterized by a hypermetabolic state, a catabolic response, and a decreased capacity to utilize substrate. Metabolic state of critically ill pediatric patients can be detected by analyzing substrate utilization.Objective: To determine the metabolic state of critically ill pediatric patients using PRISM III Score, blood glucose level, and urinary nitrogen excretion.Methods: A cross sectional study was conducted in Pediatric Intensive Care Unit (PICU) of Cipto Mangunkusumo Hospital Jakarta between May and September 2006. All admitted PICU patients, except those with renal or hepatic failure, burns, diabetic ketoacidosis and died in the first 24 hours, were included. PRISM III Scores were measured in the first 24-hours of admission. Blood glucose level was observed at random within the first hour of hospitalization, while urinary nitrogen excretion was measured using urine collected in the second 24-hours of admission.Results: Fifteen cases were eligible for this study. Their age ranged between 1 to 96 months. The median (01 ;03) for PRISM III Scores, random blood glucose, and urinary nitrogen excretion were 6.0 (3.0;11.0), 123.0 (100.0;227.0), and 279.4 (124.3;408.4) mg/dL, respectively. There were no significant correlations between PRISM III Scores and random blood glucose (r=0.40;p=0.14), and between PRISM III Scores and urinary nitrogen excretion (r = -0.27;p = 0.33). There was significant correlation between random blood glucose and urinary nitrogen excretion (r=0.56;p=0.03).Conclusion: Critically ill pediatric patients had a catabolic state that had no association with PRISM III Scores. Random blood glucose level within the first hour of admission predicted the increasing protein catabolism.Key words: critically ill pediatric patients - blood glucose level - urinary nitrogen excretion - PRISM III Score.
oai:jurnal.ugm.ac.id:article/3009
2015-11-18T02:05:51Z
bik:ART
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https://journal.ugm.ac.id/bik/article/view/3009
2015-11-18T02:05:51Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 02 (2008)
Psychosocial stress and bacterial vaginosis in adult women
Ova Emilia, Ova Emilia
2015-11-18 09:05:51
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3009
id
Objectlve:The purpose of this study was to assess the association of bacterial vaginosis with psychosocial stress with in adult women.Methods: A cross sectional design was used. Adult women (n = 320) aged 15 to 44 years were recruited at routine health care visits. Assessments were conducted on clinical symptoms, and an extensive selfreport interview.Results: Psychosocial stress was associated with overall prevalence of bacterial vaginosis (ratio prevalence, 1.22; 95% CI, 1.13-1.34). Factors that may influence the association were age, frequency of douching, frequency of vaginal intercourse and use of hormonal contraceptive. The association was not changed appreciably by the control for behavioral and demographic characteristics.Conclusion: Increased psychosocial stress is associated with greater bacterial vaginosis prevalence independent of other risk factors.Key words: bacterial vaginosis - stress - immune functioning
oai:jurnal.ugm.ac.id:article/3010
2015-11-18T02:05:51Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3010
2015-11-18T02:05:51Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 02 (2008)
Diagnostic test of mammography in a palpable mass of the breast
Bambang Udji Djoko Rianto, Rosalia Sri Sulistijawati Henry Kusumo Husodoputro
2015-11-18 09:05:51
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3010
id
Background: Breast cancer is the most common cancer in Indonesia after cervical cancer. The diagnosis of breast cancer is based on triple diagnostics, clinical diagnostic, mammograph, and fine needle aspiration biopsy (FNAB). Mammograph is a non-invasive radiologic imaging to demonstrate microcalcification as an early sign of breast cancer.Objective: The aim of this study was to measure the validity of mammograph in a palpable mass of the breast.Methods: Study design for this study was a diagnostic test that compared mammograph with histopatology result as the gold standard. Study was performed between March 2006 and September 2007 in Sardjito General Hospital, Yogyakarta. There were 79 palpable masses of the breast that were included as the subjects of this study. They were mammographed and examined histopatologically. Descriptive analysis with McNemar non parametric test with 2x2 table was performed by a computer software program to calculate sensitivity, specificity, positive predictive value (PPV), negative predictive value (NPV), positivelikelihood ratio (positive LR) and negative likelihood ratio (negative LR). .Results: Sensitivity, specificity, PPV, NPV, positive LR and negative LR of mammograph in diagnosing a breast palpable mass were 87.18%,87.50%,87.18%,87,50%,7, and 0.146, respectively Conclusion: Mammograph had a high validity in diagnosing breast palpable masses.Key words: breast palpable mass - mammograph - diagnostic test - validity.
oai:jurnal.ugm.ac.id:article/3011
2015-11-18T02:05:43Z
bik:ART
v2
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2015-11-18T02:05:43Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 03 (2008)
Retinal sensitivity changes after retinal surgery between new onset and long-term rhegmatogenous retinal detachment
Retno Ekantini, Rose Angela Nurini Agni
2015-11-18 09:05:43
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3011
id
Background: Rhegmatous retinal detachment is an emergency case which will had to blindness if it is not managed properly. It could managed mostly by surgery. Many factors influence the success of the management.Objective: To know the difference of central retinal sensitivity changes after retinal surgery in new onset and long-term rhegmatogenous retinal detachment (RRD)Methods: A prospective cohort study. Eyes with RRD which showed anatomically successful surgery were included in this study. RRD developed more than 24 days prior to surgery was considered as long-term RRD. Sample size was calculated based on the assumption that clinically significant central retinal sensitivity change was 5 decibels. Central retinal sensitivity was examined with Goldmann manual kinetic perimetry before and 3 months after surgery.Results: There were 35 eyes included in this study, consisted of 19 new onset RRDs and 16 long-term RRDs. The macula were involved in all of the subjects. The extent of retinal detachment ranged between 4 clock hours and 12 clock hours. PVR was found in 4 eyes. Two eyes (5.7%) underwent scleral buckling (SB), one eye (2.9%) underwent SB and posterior vitrectomy using silicone oil, and 32 eyes (91.4%) underwent SB and posterior vitrectomy using gas. Retinotomy was performed in 2 eyes and internal limiting membrane (lLM) peeling in 1 eye. Three months after the surgery, the mean change of central retinal sensitivity for both groups was 8.60 :I: 6.02 dB (range: -3 dB to + 23 dB), the new RRD was 6.9 :I: 5.57 dB, and the long-term RRD (p= 0.067) was 10.63 :I: 6.07 dB.Conclusion: The difference of central retinal sensitivity changes between new onset and long-term RRD was not significant clinically and statistically. However, both groups showed improvement of central retinal sensitivity.Keywords: retinal sensitivity - new onset rhegmatogenous retinal detachment - long-term rhegmatogenous retinal detachment - Goldmann manual kinetic perimeter
oai:jurnal.ugm.ac.id:article/3012
2015-11-18T02:05:51Z
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https://journal.ugm.ac.id/bik/article/view/3012
2015-11-18T02:05:51Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 02 (2008)
Natural Killer (NK) cells and severity of dengue infection among children hospitalized in Dr. Sardjito Hospital
Ida Savitri Laksanawati, Umi S. Intansari
2015-11-18 09:05:51
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3012
id
Background: Dengue infection isONE OF the most frequent infectious diseases in Indonesia. The manifestation of dengue infections varies from asymptomatic to live-threatening, dengue shock syndrome. It is hypothesized that immune responses contribute to immunopathogenesis of severe dengue infection. Natural killer INK) cells are innate lymphocytes which play a crucial role in anti-pathogen host defense and are particularly important in mediating resistance to viral infections. However, only a few studies of NK cells have been conducted in vivo, especially in Indonesia.ObJectives: The objectives of this study were to find the correlation between the number of NK cells with severity of the disease, and to find out the profile of NK cells absolute numbers in acute and convalescent dengue infection patients among children hospitalized n Dr. Sardjito hospitalMethods: An observational prospective study was conducted. Clinical and laboratory data were followed from the day of admission until the day of discharge. The severity of the disease was graded according to the WHO criteria for Dengue Hemorrhagic Fever IDHF). The absolute number of NK cells was measured by flow cytometer using monoclonal antibody anti-CD3, anti-CD16 + 56, and anti-CD45, with dual platform method. Student t-test was used to find out the difference of NK cell absolute number between acute and convalescent phases. The correlation between NK cells and the severity of the disease was analyzed using Spearman correlation test.Results: NK cell absolute number tended to be lower in acute compared to convalescent phase, but the difference was not statistically significant. There was an inverse correlation between the NK cell absolute number and the severity of dengue infection Ir=-O.322; p=O.017).Conclusion: Absolute number of NK cells were decreased during acute phase of dengue infection, and the decrease was more pronounced in severe than in mild dengue infection.Key words: NK cells absolute number - mild and severe dengue infection - acute and convalescent phases
oai:jurnal.ugm.ac.id:article/3013
2015-11-18T02:05:59Z
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2015-11-18T02:05:59Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 01 (2008)
The Effecttiveness of topical cyclosporine 0.05% compared to topical fluorometholone 0.1% in the therapy of vernal keratoconjunctivitis
Agus Supartono, Edy Ariston Suhardjo
2015-11-18 09:05:59
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3013
id
Background: Vernal keratoconjunctivitis (VKCI is a chronic and often severe form of bilateral tarsal and or bulbar conjunctivitis. Several studies have reported that topical corticosteroid therapy was effective and cyclosporine was very effective in the treatment of moderate and severe VKC. In this study we wanted to know the effectiveness of topical cyclosporine 0.05% compared to topical fluorometholone 0.1 % in VKCObjective: The objective of this research was to know the effectiveness and side effect on the raise of intraocular pressure of 0.05% cyclosporine eyedrop compared 0.1 % fluorometholone on patients suffered from vernal keratoconjunctivitisMethods: A double blind randomized controlled trial was performed on the subjects suffered from vernal keratoconjunctivitis who fulfilled inclusion and exclusion criteria. The subjects were allocated into two groups by block randomization. The first group was given 0.05% cyclosporine eye drops, whereas on the other group was given 0.1 % fluorometholone eye drop. All the subjects were examined for the clinical symptoms and signs, intraocular pressure, eosinophil count with Giemsa staining before and one month after treatment. The cyclosporine group consisted of 21 subjects whereas the 0.1 % fluorometholone group was 22 subjects. The ages of both groups were within the age range of 4-36 years old.Results: Both groups showed improvement of symptoms and signs and were not statistically significant difference (p = 0.311; 95% CI: 0.102-0.3131. xxxx The use of 0.1% fluorometholone eyedrops for one month was not associated with any increase of lOP compared to 0.05% cyclosporine (p = 0.11 and p = 0.12). There was improvement in the eosinophil count in both groups and no statistical significant difference (p = 0.301. The side effect of pain was more in 0.05% cyclosporine administration and the difference was statistically significant (p=0.0011 with number needed to harm (NNHI of 2.69.Conclusion: It was concluded that the use of 0.05% cyclosporine eyedrop was as effective as the use of 0.1 % fluorometholone but the side effect of pain occured more often. No statistical difference was found between either the intraocular pressure increase or the eosinophil count in the use of 0.05% cyclosporine eyedrop compared to 0.1 % fluorometholone.Keywords: Vernal keratoconjunctivitis - cyclosporine 0.05% - fluorometholone 0.1 % - side effects, eosinophil count
oai:jurnal.ugm.ac.id:article/3014
2015-11-18T02:05:59Z
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https://journal.ugm.ac.id/bik/article/view/3014
2015-11-18T02:05:59Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 01 (2008)
High number of granzyme B expressing CTLs predicts worst prognosis of nasopharygeal carcinoma patients
Harijadi, Harijadi
2015-11-18 09:05:59
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3014
id
Background: Nasopharyngeal carcinoma (NPC) characteristically harbors many tumor-infiltrating lymphocytes. In biopsies of Hodgkin or anaplastic large cell lymphoma many activated CTLs are related to a very poor clinical outcome, suggesting that in these cases with a strong CTL mediated anti-tumor cell response, selection occurs for tumor cells that have become resistant to CTL and chemo and/or radiotherapy induced apoptosis. Only activated CTLs and natural killer cells express granzyme B.Objective. Since, similar to lymphomas, the prognosis of NPC patients depends primarily on the sensitivity of tumor cells to radio- and/or chemotherapy, this study investigated whether the presence of many tumor-infiltrating activated CTLs in tumor biopsies also predicts poor prognosis in NPC patients.Methods: The study investigated 39 specimens of Indonesian NPC patients that fulfilled the following criteria; no evidence of distant metastases at the time of diagnosis, and complete remission following complete radio- and/or chemotherapy. Number of tumor-infiltrating activated CTLs was detected using a combination of antibodies against granzyme Band CD3, CD8 and CD56.Results: The presence of a high number of tumor infiltrating activated CTLs expressing granzyme B appeared to be a very strong predictor of a rapid fatal clinical outcome. Its prognostic value was stronger than and independent from the other prognostic makers; age and clinical TNM stage at presentation. Prognosis was found to decline strongly with increasing percentage of activated CTLs. The most informative cut-off value was found to be 25%. The median overall survival time of patients with
oai:jurnal.ugm.ac.id:article/3015
2015-11-18T02:05:59Z
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https://journal.ugm.ac.id/bik/article/view/3015
2015-11-18T02:05:59Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 01 (2008)
Herpes simplex encephalitis (A review)
Satiti Retno Pudjiati, Herning Setijowati
2015-11-18 09:05:59
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3015
id
Herpes simplex encephalitis (HSEI is an inflammation of the brain caused by herpes simpleks virus (HSVI. HSE includes serious types. HSE can influence all age group, but most common in those under 20 and over 40 years old. Clinical diagnosis based on clinical presentation, laboratory examination of cerebrospinal fluid (CSFI, even by computerized tomography scan (CT-scan)/magnetic resonance imaging (MRII, electro encephalogram (EEGI is difficult that resulted in nonspecific diagnosis. Polymerase chain reaction (PCRI, and brain biopsy are the best diagnostic test. The main treatment of HSE is non spesific i.e. acyclovir. An early management may decrease the risk of mortality from 80% - 25%. While without treatment the mortality.can reach 70-80% and the prognosis might unfavourable.Key words: encephalitis - HSE - CSF - CT-scan - MRI - EEG
oai:jurnal.ugm.ac.id:article/3016
2015-11-18T02:05:59Z
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https://journal.ugm.ac.id/bik/article/view/3016
2015-11-18T02:05:59Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 01 (2008)
Diabetic retinopathy diagnostic with ophthalmoscope by trained doctor
Hartono, Imam Masduki Angela Nurini Agni
2015-11-18 09:05:59
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3016
id
Introduction. Diabetes mellitus patient is in high risk to be complicated by retinopathy. Visual loss and decrease of visual acuity become a significant social burden. Early detection is essensial for treatment. Ophthalmoscopy was highly effective for screening, easily used and accessed by ophthalmologist and other physician and has been applied in many developed countriesPurpose : This study was aimed to know the validity of ophthalmoscopic test done by a trained doctor in diagnosing diabetic retinopathy..Materials and methods : The subjects were diabetic patients without cataracts. The examiner was a resident of Internal Medicine Department who had been trained in ophthalmoscopy. After the training, the resident was assessed and the result was compared with the trainer and showed agreement with kappa 1.00.Results: The study was done from February to May 2007. Total subjects were 98 patients (196 eyes I consisted of 50 females (51%1 and 48 males (49%1 with mean age of 61 :t7.92 years old. Mean diabetes duration was 6.6:t 5.94 years and all of them were diabetes type 2. Some patients had hypertension (44.9%1, dyslipidemia (17.7%1, heart disease (6.1%), and kidney disease (5.1%1. From 196 eyes, 6 (6.1 %1 eyes had cataract and were excluded. Mean visual acuity was 0.7726 :to.31 08. Diabetic retinopathy was found in 41 eyes among 190 eyes (21.57%1, consisted of 22 eyes with mild NPDR (53.65%1, 7 eyes with moderate NPDR (17.07%), 7 eyes with severe NPDR (17.07%1, 5 eyes with advance PDR (12.19%1. Sensitivity, specificity, PPV, NPV and accuracy resulted by 5 hour trained doctor examinations were 63.41%, 67.78%, 36.62%, 67.78%, and 66.78% respectively R (+1 and LR (-I were 1.968 and 0.5397, respectively.Conclusion: Direct ophthalmoscopic examination by a trained doctor to detect retinopathy is promisalble in Indonesia.Keywords : diabetic retinopathy, direct ophthalmoscopy, diagnostic value, traineddoctor
oai:jurnal.ugm.ac.id:article/3017
2015-11-18T02:05:59Z
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https://journal.ugm.ac.id/bik/article/view/3017
2015-11-18T02:05:59Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 01 (2008)
Validity of BI-RADS System Mammography to detect breast cancer at Dr Sardjito Hospital Yogyakarta
Teguh Aryandono, Indrasto Histopaedianto Lina Choridah
2015-11-18 09:05:59
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3017
id
Background: Breast cancer is the second most common cancer among women worldwide and the sixth common cause of mortality in Indonesia. The diagnosis of breast cancer may be established by physical and imaging examinations, but definitive diagnosis should be verified by histopathologic examination. Mammography is an easy non-invasive imaging method to detect breast cancer with good accuracy. The lack of uniformity in mammography reporting has led to confusion as to the interpretation and management recommendation. The American College of Radiology Breast Imaging Reporting and Data System (BIRADS) has provided a standardized reporting system for mammography. The validity of this system at Dr Sardjito Hospital Yogyakarta has not been evaluated.Objective: To know the sensitivity, specificity, positive and negative predictive values, and accuracy of the BI-RADS System Mammography in diagnosing breast cancer.Methods: This study was a diagnostic test with cross sectional design on breast cancer diagnosis based on BI-RADS system mammography. All medical record files of patients with breast lump who underwent mammography and histopatologic examination, at Dr Sardjito Hospital Yogyakarta between December 2003-March 2008, were included in the analysis.Results: BI-RADS System Mammography at Dr Sardjito Hospital Yogyakarta had sensitivity of 87.5%, specificity of 93.7%, positive predictive value of 96.5%, negative predictive value 78.95%, positive likelihood ratio 14, negative likelihood ratio 0.13, prevalence 66.6%, and accuracy 89.6%.Conclusion: Mammography examination using BI-RADS System in Sardjito Hospital Yogyakarta was favourable in detecting breastcancer.Key words: breast cancer - BI-RADS mammography - histopathology - diagnostic value
oai:jurnal.ugm.ac.id:article/3018
2015-11-18T02:05:59Z
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2015-11-18T02:05:59Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 01 (2008)
Effect of vitamin C and vitamin E supplementation on lipid peroxide of lactating woman
Prasetyastuti, Prasetyastuti; Laboratory of Biochemistry Faculty of Medicine, Gadjah Mada University, Yogyakarta
Sri Sunarsih, Endang; Faculty of Medicine, Diponegoro University, Semarang
2015-11-18 09:05:59
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3018
id
Background: Vitamin C and vitamin E are antioxidants that trapp free radical that is continuously formed in aerobic organism as an effect of aerobic respiration in mitochondria and substrate oxidation. The quantity and quality of breast feeding (ASH are influenced by nutritional status and health of the mother. The ASI vitamin C level is very much related to everyday intake and plasma vitamin C of the mother.Objective: To know the effect of vitamin C and vitamin E supplementation on peroxide lipid (MDA) of lactating women.Methods: This was pretest and post test experimental design. The subjects were twenty three lactating women who live in Pakem District, Sleman 19-38 year olds they received 75 mg vitamin C and 2 mg vitamin E every two days for a period of one month. Blood sample was taken from cubit vein before and after supplementation. Determination of vitamin E level used spectrofluorometer, whereas vitamin C and MDA level were determined by spectrophotometer. The data was analyzed with Pearson correlation test Results: the correlation of vitamin C vs MDA; vitamin E vs MDA and vitamin C together with vitamin E vs MDA were r - 0.528; r square 0.276 and p = 0.05; r -0.671, r square 0.451, p = 0.009 and r 0.690; r square 0.476 and p= 0.028, respectivelyConclusion: the effect of vitamin E to MDA was stronger than vitamin CKeyword.: vitamin C - vitamin E - peroxide lipid - free radical - lactating mother
oai:jurnal.ugm.ac.id:article/3019
2015-11-18T02:05:59Z
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2015-11-18T02:05:59Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 01 (2008)
The role of red blood cell indices to identify iron deficiency in anemic pulmonary tuberculosis patient
Ratnaningsih, Tri
2015-11-18 09:05:59
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3019
id
Background: iron deficiency anemia (IDA) is often associated with infectionlinflammation disease, such as pulmonary tuberculosis. The biochemical parameters tests of iron metabolism are expensive relatively and not always available in some health centers. Objective: The aim of this study was to know the role of red blood cell indices to identify iron deficiency on pulmonary tuberculosis patient with anemia using a sTfR-based biochemical classification of ACD and ACD with iron deficiency (COMB!). Methods: The patient population consisted of 68 consecutive anemic pulmonary tuberculosis patients at the Sardjito Hospital and Balai Pengobatan Penyakit Paru-Paru 0.1. Yogyakarta, Indonesia. Result: Of 68 subjects, 53 had anemia of chronic disease (ACD) and 15 had iron deficiency anemia (COMBI). It was founded a significant difference between ACD group and COMBI group. There were significant correlations between red blood cell indices and sTfR. The most valid parameter to diagnose 10 is MCHC < 31.55 g/dL (Sn= 67%, Sp= 75%), followed by MCV < 81.65 fL (Sn= 62%, Sp= 73%), MCH < 25.75 pg (Sn= 67%, Sp= 70%), and lastly ROW> 14.95 (Sn= 73%, 57%). The diagnostic performances of this parameter at levels commonly used: MCV < 76 fL (Sn= 13%, Sp= 85%), MCH < 27 pg (Sn= 100%, Sp= 45%), MCHC < 31g/dL (Sn= 13%, Sp= 85%), and RDW > 15 (Sn= 67%, 60%). Conclusion: The usage of red blood cell indices can help us to identify iron deficiency in anemic pulmonary tuberculosis patients. For practical reason, more convenience if we use the value that is widely accepted. However, the red blood cell indices cannot be used solely, but must be combined for a greater accuracy.
oai:jurnal.ugm.ac.id:article/3020
2015-11-18T02:05:59Z
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2015-11-18T02:05:59Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 40, No 01 (2008)
The Combination of suprakeloidal flap and pulsed light heat energy in keloid management: a Case report
Wirohadidjojo, Yohanes Widodo
Etnawati, Kristiana
Adiwinarni, Dwi Retno
2015-11-18 09:05:59
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3020
id
The role of chronic tissue hypoxia in the keloid patho-mechanism has been widely accepted. Whereas, the pulsed-light heat energy (LHE) has been developed which has the capacity to generate reactive oxygen species on exposed skin. Although the supra keloidal flap technique has a high recurrence rate, it was used because of its capacity to prevent suturing hypoxia, thereby the formation of lager recurrent keloid after surgery.The combination of supra keloidal flap and pulsed light heat energy was done I the treatment of postvaricella keloid on the right ear lobe of a 9 year old girl. The keloid was excised two times a year ago, but observation one month after the surgery showed a recurrent larger keloid. The performance of supra keloidal technique followed by pulsed-light heat energy treatment in dose 2.5 J/cm2, was administered on day 3r
oai:jurnal.ugm.ac.id:article/3021
2015-11-18T02:06:06Z
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2015-11-18T02:06:06Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 03 (2007)
Nonmosaic 45, XO karyotype in a woman with Turner syndrome without any cognitive, psychosocial or behavioral deficiencies (A Case report)
Djaswadi Dasuki, Enny S Pamuji
2015-11-18 09:06:06
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3021
id
A 22 year old woman with primary amenorrhea came to The Reproductive Endocrinology and Infertility Centre, University of Gadjah Mada, Dr.Sardjito Hospital. She had no abnormalities in cognitive, psychosocial or behavioral capacities, she had almost completed her university degree. On examination she had no secondary sexual development, no pathognomonic abnormal phenotype related to abnormal karyotype. There was no indication of family history associated ,;pecifically to the abnormal karyotype, phenotype feature of Turner syndrome and a specificThe patient karyotype analysis of blood sampling indicated 45XO, and it failed to demonstrate any mosaicism. Laparoscopic examination revealed a hypoplastic-lobulated-unicorns uterus, as streak gonad at the left side and no ovary at the other side.Keywords: Turner syndrome, non behavioral deficiencies. nonmosaic 45, XO karyotype, normal hormone level
oai:jurnal.ugm.ac.id:article/3022
2015-11-18T02:06:06Z
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2015-11-18T02:06:06Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 03 (2007)
Expression of CD5, CD1 0, Bcl-2 and Bcl-6 in diffuse large B cell lymphoma based on International Prognostic Index
Guntersah, Indrawati Tody
2015-11-18 09:06:06
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3022
id
Background: Diffuse large B cell lymphoma is the most common type of non-Hodgkin lymphoma. This tumor has heterogeneous clinical feature, morphology, genetic and molecular alterations. Diffuse large B cell lymphoma with a germinal centre ( GC ) pattern of gene expression including CD10 and Bcl-6 has a more favorable outcome. CD5 and Bcl-2 expression has been shown to have an adverse effect on the outcome in diffuse large B cell lymphomaObjective: To investigate the difference of CD5, CDlO, Bcl-2 and Bcl-6 expression based on International Prognostic Index IIPI) in diffuse large B cell lymphomaMethods: Fourty five cases of B cell diffuse large B cell lymphoma from embedding parafin tissue were constituted the basis of the study. Immunohistochemical examination using CD20, CD5, CD10, Bcl-2 and Bcl-6 monoclonal antibody was done. The difference of CD5, CD10, Bcl-2 and Bcl-6 expression between high and low IPI of diffuse large B cell lymphoma were analized by chi square testResult: There Vlias significant difference between the number of cases with positivity of CD5 and CD10 expression in high and low IPI of diffuse large B cell lymphoma. The result supported that immunophenotyping related B cell differentiation can help to predict clinical behavior and prognosis in diffuse large B cell lymphoma.Conclusion: Conclusion: There was significant difference between the number of cases with positivity of CD5 and CD10 expression in high and low IPI of diffuse large B cell lymphoma. The result supported that immunophenotyping related B cell differentiation can help to predict clinical behavior and prognosis in diffuse large B cell lymphoma.Key words : diffuse large B cell lymphoma, CD5, CD 10, IPI
oai:jurnal.ugm.ac.id:article/3023
2015-11-18T02:06:06Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3023
2015-11-18T02:06:06Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 03 (2007)
he correlation between HPV infection and p53, pRb, KI-67 as well as tunnel expression of cervical carcinoma
Endang Soetristi, Irianiwati Ahmad Ghozali
2015-11-18 09:06:06
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3023
id
Background: HPV infection has an important role in cell proliferation and apoptosis of malignant neoplasia through the binding of their E6 and E7 protein to the p53 and pRb. In cervical cancer the role of HPV infection in cells proliferation and apoptosis has not yet been defined up to now, since mutation of p53 gene and Rb gene in cervical cancer is very rare.Methodes: Thirty-eight block parafin cases of squamous cell carcinoma of the cervix were stained immunohistochemically with polyclonal antibody against HPV and monoclonal antibody against p53, Rb, Ki-67 and Tunnel. with chromogen DAB and counterstain Hematoxyllin Meyer. The expression of HPV, p53, pRb, Ki-67 and Tunnel were counted based on the number of positive cells/100 tumor cells in five microscopic fields. The correlation between HPV expression and p53, pRb, Ki-67, Tunnel expression respectively were analyzed with Pearson correlation analysis.Results: There was a significant correlation between HPV expression with either p53 expressionR: 0.491, P:0.002) and or Ki-67 expression (R:0.397, P:0.014), but there was no significant correlation between HPV expression with pRB expression (R:0.3000, P:0.067) and Tunnel expression ( R:O,888, P:0,599).Conclusion: This research showed that HPV infection had important role in cell proliferation but not in cell apoptosis of cervical cancer.Key words: HPV, proliferation, apoptosis, p53, pRb, Ki-67, Tunnel, cervical carcinoma
oai:jurnal.ugm.ac.id:article/3024
2015-11-18T02:06:06Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3024
2015-11-18T02:06:06Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 03 (2007)
Comparasion of Sport Urine Protein Creatinine From Three Different Time Urine Samples in Children With Primary Nephrotic
Pungky Ardani, Jusuf Hartanto Mohammad Juffrie
2015-11-18 09:06:06
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3024
id
Background: First voiding of morning urine protein/creatinine ratio on a single voided urine sample correlates well with 24 hour urine collection and can be used to detect and monitor proteinuria in children. Objective: Aim of this study was to determine whether the random urine samples can be used as reliable indicator for spot urine protein/creatinine ratio.Methods: 40 primary nephrotic syndrome children aged 1-16 years admitted to the Department of Pediatric, Dr Sardjito hospital were included in this study. Urine samples from three different times were collected: first voiding of morning urine, noon voiding and night voiding. The three samples were analyzed for protein level, creatinine level and protein/creatinine ratio of spot urine. The protein/creatinine ratio was compared and correlation was calculated according to the three different time collections.Results: There was no significant difference of noon and night protein/creatinine ratio compared with first voiding of morning urine protein creatinine ratio (p> 0.05). The correlation of protein/creatinine ratio between the three samples was significantly high (r=0.95, 0.96 and 0.98, p < 0.01).Conclusions: For quantifying proteinuria, there is convincing evidence that the random urine samples can be used to predict the level of protein excretion as measured in a 24-hour sample.Keywords: Proteinuria, protein, creatinine ratio, primary nephrotic syndrome
oai:jurnal.ugm.ac.id:article/3025
2015-11-18T02:06:06Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3025
2015-11-18T02:06:06Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 03 (2007)
ytotoxic effect of n-hexane insoluble fraction obtained from kloroform extract of kembang bulan leaves [Tithonia diversifolia (Hemsley) A. Gray] on HeLa cells and mechanism detection by Hoechst 33342
Zany sari Vindawati, Rita Rakhmawati, Mae S.H Wahyuningsih, Rul Afiyah Syarif,
2015-11-18 09:06:06
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3025
id
Background: Tithonia diversifolia (Hemsley) A.Gray is a plant traditionally used to cure various diseases. The previous study indicated that n-hexane insoluble fraction of chloroform extract of T. diversifolia's leaves showed cytotoxic effect on Hela cells. Separation of n-hexane insoluble fraction into several fractions was conducted in order to simplify the components. Therefore, compounds responsible for cytotoxiceffect and their mechanism of action can be determined. .Objectives: To determine fraction having the most cytotoxic effect and its mechanism of action obtained from n-hexane insoluble fractions of T.diversifolia leaves on Hela cells.Materials and methods: Fractionation of n-hexane insoluble fraction from chloroform extract of T. diversifolia leaves was done by using vacuum liquid chromatography. By MTT assay, the cytotoxic effect of fractions on Hela cells in vitro was determined. and doxorubicin was used as positive control. Each group was tested for 3 times In = 3) and the growth inhibition percentage of Hela cells was counted. The data was analyzed by probit regression, and mechanism of action of the active fraction is conducted by staining with hoechst 33342.Results: Fractionation gave five fractions IF1-5). The ICso levels obtained of those fractions were : F1 having ICso = 32.168 :!: 12.513; F2 = 11.416 :!: 0.535; F3 III = 6.223 :!: 1.303; F4 = 94.308 :!: 7.843; and F5 = 41.740 :!: 6.381Ig/ml; while the ICso value of doxorubicin was 0.241 :!: 0.170 Iglml. Conclusions: Fraction 3 of the n-hexane insoluble displayed the highest cytotoxic effect on Hela cells with ICso= 6.223:!:: 1.303 Iglml. Fraction 3 at the concentration of 3.93 uglml caused chromatin condensation and fragmentation of DNA on Hela cells.Key words: T. diversifolia, cytotoxic effect. Hela cells, insoluble n-hexane fractions
oai:jurnal.ugm.ac.id:article/3026
2015-11-18T02:06:06Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3026
2015-11-18T02:06:06Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 03 (2007)
Relation between vitamin C and vitamin E levels with malondialdehyde in elderly
Irianti Puji Lestari, Prasetyastuti Sunarti, Dyah Arum Retnaningstyas
2015-11-18 09:06:06
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3026
id
Background: Aging is a natural process during life. One of the aging theories is related to free radical production. Increase of free radical production can trigger lipid peroxidation that result in peroxide lipid such as malondialdehyde (MDA). The lipid peroxidation can be inhibited by body defence system such 8S vitamin C and vitamin E.ObJective: to understand the relation between plasma vitamin C, vitamin E and MDA levels in elderly Method: This was an observational research with cross sectional design. The subjects were thirty two elderly who live in Pakem district, Sleman. Determination of vitamin C and peroxide lipid level used spectrophotometer, whereas vitamin E level was determined by spectrofluorometer.Reaulta : The concentration of vitamin C , vitamin E and MDA of the subjects were 0.50 + 0.26 mglml, 26.38 ..:!:. 7.95 pg/ml and 0.23 ..:!:. 0.13 nmol/ml respectively. The correlation of both vitamin C vs MDA and vitamin E vs MDA were not significant, r = -0.012 and -0,108, respectively.Conclualon: There were no correlation between vitamin C, vitamin E and MDA levels.Keyword: elderly ; free radical;vitamin C; vitamin E; malondialdehyde.
oai:jurnal.ugm.ac.id:article/3029
2015-11-18T02:06:10Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3029
2015-11-18T02:06:10Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 02 (2007)
The Effect of Topical Extravirgin Olive Oil on Ultraviolet B-Induced 8-Hydroxy-2'-Deoxyguanosine Formation in Human Skin
Arief Budiyanto, Irianiwati, Catharina Sagita Yohanes Widodo Wirohadidjojo
2015-11-18 09:06:10
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3029
id
Background: Ultraviolet B (UVBI may cause DNA damage due to photooxydative reaction and form 8hydroxy-2'-deoxyguanosine (8-0HdGI as a genotoxic product. Topically extra virgin olive oil (EVOOI can decrease 8-0HdG formation among UVB irradiated-hairless mice epidermal cells. Such effect in human skin has never been discovered.Objective: To know the effect of topically EVOO in UVB induced-8-0HdG formation in human epidermal cells.Method: A simple experiment was performed on cultured human foreskins. The foreskins were divided into 3 groups: group was treated with 100mJ/cm2 UVB + topical EVOO, group B with 100mJ/cm2 UVB, and group C as control. All skin was cultured in completed DMEM and fixation was performed at one and 24 hours. 8-0HdG expression was determined immunohistochemically with antibody anti 8-0HdG and measured based on histogram of selected colour of Adobe photoshop.Results: The mean of 8-0HdG expression in experimental groups: (8.02:t 13.81, (26.95:t 23.231. and 14.96:t 7.141, respectively (p =0.0001 at an hour; (14.91 :t30.63I, (37.58:t 25.631. and (8.76:t 10.941 respectively (p = 0.0001 at 24 hours.Conclusion: Extra virgin olive oil was proved to reduce the 8-0HdG formation in exposed human skin-UVB. and may be developed to be anti-photocarcinogenesis active ingredient topically.Kata kunci:Extra virgin olive oil, UVB, 8-OHdG, human Skin
oai:jurnal.ugm.ac.id:article/3030
2015-11-18T02:06:10Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3030
2015-11-18T02:06:10Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 02 (2007)
Pharmacokinetic profile of iron serum after ingestion of ferrous sulfate tablet 2 300 mg taken shortly after meal and 2 hours after meal in women with iron deficiency anaemia
L.Hakim, E.Kristin M.Hakimi, S.K.Soejono
2015-11-18 09:06:10
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3030
id
Background: Iron supplementation is a common strategy used to control iron deficiency anaemia in developing countries. Despite of that, the prevalence of anaemia is still high. Several factors causing the failure of iron supplementation program were reported. The increase in haemoglobin concentration after iron supplementation was determined by several factors, that is, the dose, iron absorption, body iron store, diet, food, and pregnancy. There had been studies on iron pharmacokinetic profile after ingestion of iron tablet taken shortly after meal compared to 2 hours after meal in healthy women, but there was no study on women with iron deficiency anaemia.Aim: The aim of this study was to find out the effect of ferrous suphate @ 300 mg taken shortly after meal and 2 hours after meal on the iron pharmacokinetic profile in women with iron deficiency anaemia.Materials and method: This study was conducted with cross-over design, so that every subject had 2 interventions with an interval (washed-out period) of 7 days. In intervention 1, ferrous sulfate tablet @ 300 mg was taken shortly after meal, and in intervention 2, the tablet was taken 2 hours after meal. Serum samples were taken serially at 0, 0.25, 0.5, 1, 1.5. 2, 2.5, 3, 4, 6, 8, 10 and 12 hours after taking iron tablets. Ferric iron concentration in serum was determined by Vitros Fe Slides method, and iron pharmacokinetic profile was determined using non-compartmental method.Result: The values of pharmacokinetic parameter from the first and second interventions were compared by t test, and the result (mean:t SEMI were: Cmox 274.25 :t 28.18 and 232.92 :t 33.25 ug/dl, T mox 3.21:t 0.30 and 4.25 :t 0.64 hours, K.0.437 :t 0.0011 and 0.3808 :t 0.0065 hour'" Kel 0.1375 :t 0.0132 and 0.1855 :t 0.0048 hour". T'/2e' 5.76 :t 0.74 and 5.81 :t 1.16 hours and AUCO-,2 2157.37 :t 264.55 and 1869.54 :t 330.60 ug/dl.hours. There are no significant differences in all pharmacokinetic parameters between both interventions.Conclusion: There are no differences in iron pharmacokinetic profiles after ferrous sulfate tablet @ 300 mg taken after meal and 2 hours after meal in women with iron deficiency anaemiaKata kunci:iron concentration, pharmacokinetics, ferrous sulfate, anemia, effect of food
oai:jurnal.ugm.ac.id:article/3031
2015-11-18T02:06:10Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3031
2015-11-18T02:06:10Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 02 (2007)
The Evaluation of Vaginal Hysterectomy Using Spinal Anaesthesia
Ibnu Pranoto, Ibnu Pranoto
2015-11-18 09:06:10
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3031
id
Background. Vaginal hysterectomy can be performed on patient under general or spinal anesthesia. There are some advantages of spinal anesthesia compared to general anesthesia, although some prefer general anesthesia. We have done frequently vaginal hysterectomy using spinal anesthesia and we are reporting our experience.Objective:To know the spinal anethesia efficency compared to general anesthesia in terms of duration of vaginal hysterectomy operation, amount of bleeding and length of stay.Design: Cross sectional descriptive studySetting: Sardjito HospitalMaterials and methods: Patients underwent vaginal hysterectomy in Dr. Sardjito Hospital from March 2005 until February 2007Results: 50 patients underwent vaginal hysterectomy that consisted of 44 patients with spinal anesthesia and 6 patients with general anesthesia. Vaginal hysterectomy with spinal anesthesia had shorter duration of operation (1.93 hours) compared to general anesthesia (1.95 hours). Patients length of stay was shorter with spinal anesthesia (5.8 days) compared to general anesthesia (9 days). The average amount of blood loss in spinal anesthesia was (250.34 mil compared to general anesthesia (625.0 mil.Conclusion: This study suggests that spinal anesthesia reduces length of hospital stay and amount of blood loss in patients with vaginal hysterectomy compared to general anesthesiaKey Words: vaginal hysterectomy, spinal anesthesia, general anesthesia, duration of operation, amount of bleeding and length of stay.
oai:jurnal.ugm.ac.id:article/3033
2015-11-18T02:06:10Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3033
2015-11-18T02:06:10Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 02 (2007)
Pigmented Gallstone in Children With Thalassemia Syndrome
Sustiyanto, Nenny SM Sumadiono
2015-11-18 09:06:10
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3033
id
Background: Pigmented gall stones in children frequently occur in spherocytosis and sickle cell anemia. The most common congenital hemolytic anemia in Indonesia is thalassemia. There is only a few studies concerning pigmented gall stones in thalassemia in the world and none in Indonesia. This study was aimed to know the prevalence of pigmented gall stones in patients with thalassemia in Pediatric Department of Dr. Sardjito Hospital.Materials and method: All patients with major thalassemia visiting Dr. Sardjito Hospital who consented to take part in the study underwent ultrasonography to identify the stone or sludge. As controls, children with no jaundice were examined.'Results. There was 8,57% gallstone in thallasemia group and none in the control group.Conclusion: The occurrence of pigment gallstone in the thalassemia syndrome children in Yogyakarta the same as in other places. The risk factors of pigment gallstone should be investigated.Key words: gall stone - prevalence - thalassemia syndrome - ultrasonography.
oai:jurnal.ugm.ac.id:article/3034
2015-11-18T02:06:10Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3034
2015-11-18T02:06:10Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 02 (2007)
The role of Various Factors in the Therapeutic Response of Calcipotriol in Mild to Moderate Plaque Type Psoriasis Patients
Hardyanto Soebono, Siti Aminah Tri Susila Estri Sunardi Radiono
2015-11-18 09:06:10
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3034
id
Background: Psoriasis is a chronic skin inflammation and proliferation disease. Natural history of psoriasis depends on the genetic, age, sex, history of treatment and psychosocial stressor. Calcipotriol is suitable for long-term therapy with good efficacy and safety and clinical response variability but its activity is influenced by ultraviolet.Objective: To know the factors that may influence treatment response of calcipotriol ointment on mild to moderate plaque type psoriasis.Method: Longitudinal study of two times daily calcipotriol ointment 0.005% treatment in the psoriasis patients. Factors that may influence the natural course (age, sex, history of psoriasis in family, history of therapy, MED, working location and psychological stressor) were identified. Evaluation of therapy was performed in the 8th weeks and based on the PASI and PDI. Correlation between treatment responses with various factors were analyzed by using student t-test, Pearson and Spearman correlations and multiple linear regression.Result: At the end of study, PASI was shown to decrease 38.45%, while PDI was 28.70% (p
oai:jurnal.ugm.ac.id:article/3035
2015-11-18T02:06:10Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3035
2015-11-18T02:06:10Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 02 (2007)
Effect of C677T Mutation in Methiylenetetrahydrofolate Reductase Gene and Blood Folate on Hypertension Risk
Mohammad Hakimi, Abdul Salam M Sofro, Sunarti Ahmad Husain Asdie
2015-11-18 09:06:10
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3035
id
Background: The prevalence of hypertension risk factor varies among race or ethnic groups. The varied prevalence is associated with culture or genetic differences or interaction between genetic and environment or nutrition factorsObjective: The aim of this study was to identify relationship between polymorphism of C677T in MTHFR gene and folate status towards the risk of hypertension among Javanese population.Methods: This study was case-control nested, in "Surveillance of Non-Communicable Diseases" in Purworejo, Central Java. The subjects were men with essential hypertension and 20-60 years old that had normal value of urine creatinine, blood glucose, cholesterol and triacylglycerol. They didn't take antihypertension drugs. The analysis of C677T polymorphism in MTHFR gene was done according to Frosst et al. (1995) method. The blood folate level was determined by Delfia-Folate Kit (PerkinElmer). The methods of Pfeiffer et al. (1999) was used to identify plasma total homocysteine level.Results: Odds ratio for subjects with CT genotype was 1.36 ICI: 95%:0.50;3.70); folate deficiency was 1.40 ICI: 95%:0.57;3.451; and both CT genotype and folate deficiency was 2.30 (CI: 95%:0.45;15.001. Homocysteine level of the cases with both folate deficiency and CT genotype was 45.28 .:!:. 30.39; and only folate deficiency was 17.94.:!:. 12.77; whereas in the control with both folate deficiency and CT genotype was 21.44.:!:. 10.71 and only folate deficiency was 11.79.:!:. 5.28.Conclusions: The effect of folate deficiency to homocysteine level and hypertension risk was slighly stronger than the genetic effect of C677T in MTHFR gene. In subjects with normal folate level, the CT genotype did not influence homocysteine level.Kata kunci:polymorphism, methylenetetrahydrofolate reductase, folate, hypertension
oai:jurnal.ugm.ac.id:article/3036
2015-11-18T02:06:10Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3036
2015-11-18T02:06:10Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 02 (2007)
The Effect of Narrow and Broad Band Ultraviolet B Onto Keloid Fibroblast-VEGF Expressions
Ishandono Dahlan, Yohanes Widodo Wirohadidjojo
2015-11-18 09:06:10
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3036
id
Background: Collagenase inactivation of keloid lesions is due to plasminogen activator inhibitors which are synthesized under stimulation of vascular endothelial growth factor (VEGF), a released protein under hypoxia conditions. On the other hand, ultraviolet B (UVB) may generate various oxidative molecules of irradiated chromophores. The effect of UVB in VEGF synthesis is unclear.ObJective. To know the effect of narrow and broad band ultraviolet-B on keloid fibroblast-VEGF expression. Materials and methods: Materials in this study were keloid materials collected from dermatosurgery and plastic surgery keloid revision. A parallel simple experiment study was performed to compare the effect of 0, 50, 75, and 100mJ/cm2 of broad band (BBUVB) as well as narrow band UVB (NBUVB) in VEGF synthesis of passage-3 keloid fibroblasts isolated from 4 patients. Samples were stained with monoclonal antibody anti VEGF. The selected DAB-brown colors of cytoplasm were computed based on Photoshop software histogram.Results: Compared to untreated group, all of various NBUVB showed a very significant (P0.05) between NBUVB and BBUVB groups.Conclusion: NBUVB as well as BBUVB can suppress VEGF synthesis among irradiated keloid fibroblasts. The 50 mJ/cm2 of NB UVB as well as 75 mJ/cm2 of BBUVB may be developed as the modality in keloid prevention or treatment.Keywords: keloid-fibroblasts, broad-band UVB, narrow-band UVB, VEGF
oai:jurnal.ugm.ac.id:article/3037
2015-11-18T02:06:13Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3037
2015-11-18T02:06:13Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 01 (2007)
Cytotoxic Activity of /V-alkyl and /V-benzyl 1,10-phenathroline derivatives in Human Cancer Cell Lines
https://journal.ugm.ac.id/bik/article/download/3037/8443
Isnatin Miladiyah, Esti Nurwening Sholikhah Mustofa
2015-11-18 09:06:13
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3037
id
Background: In our study on the antiplasmodial activity of 1,1 O-phenanthroline derivatives, we found some compounds possessing a potential cytotoxic in normal cell line.Objective: In this study we tested these derivatives in human cancer lines in order to know their in vitro anticancer activity.Materials and methods: Six derivatives of 1,1 O-phenanthroline, 4 derivatives of N-alkyl and 2 derivatives of N-benzyl 1,1 O-phenanthroline were tested on two human cells cancer, myeloma (NS-1) and HeLa cells line. Cytotoxic activity was evaluated by trypan blue exclusion assay and their activity was expressed by the concentration inhibiting 50% of the cell growth (lCso)' The ICso of each compound was determined by probit analysis.Results: The results showed that the ICso values of 1,1 O-phenanthroline derivatives ranged from 4.68 to 15.63 pM on myeloma cell and from 2.82 to 16.89 pM on HeLa cell. The 4-Bromo-3(2-bromoetyll-2metyl-1 , 1 O-phenantroline (3) and (1 )-N-(4-benzyloxy-3-methoxy-benzyll-1 ,1 O-phenanthrolinium chloride (6) with ICso values ranged from 4.68 to 4.72 pM on myeloma cell showed the same (p>0.05) cytotoxicity with doxorubicin, with ICso values ranged from 2.82 to 3.08 pM on HeLa cell showed the higher (p
oai:jurnal.ugm.ac.id:article/3038
2015-11-18T02:06:13Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3038
2015-11-18T02:06:13Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 01 (2007)
Sexual dimorphism of pelvic girdle: pelvimetry and pelvic types in Javanese
Etty Indriati, Etty Indriati
2015-11-18 09:06:13
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3038
id
Background: Each population has certain phenotypic traits that are unique compared to other populations. Pelvis's size and morphology are two of them. A western formula of obstetric conjugate = diagonal conjugate - 1.5 to 2.0 em; may not be applicable to Javanese population; and sexual traits in Western pelves may not entirely be the same with Indonesians.Objective: to get a formula for obstetric conjugate, and conduct various pelvic measurements in order to find out the metric pattern as well as the distribution of pelvic types in Javanese.Material and Methods: 27 pelves consisting of 6 females and 21 males housed at the Department of Anatomy, Embryology and Anthropology were studied by connecting the sacrum with the right and left pelvis forming pelvic girdles. Superior inlet, diagonal conjugate, angle of greater sciatic notch, diameter and width of pelves were among the metric studied.Results: Widely used traits such as greater sciatic notch that are used in Western literature to differentiate male and female pelves in Caucasian is not consistent for Javanese pelves. The most consistent skeletal traits for sexing female pelvis are wide subpubic angle (> 75 degree), preauricular sulci, flat posterior pubic symphisis, and short ischiopubic rami. The common type of ginecoyd pelvis in Javanese (48%) is in accord with western pelvis reaching 50%. Compared to the western obstetric conjugate, Javanese obstetric conjugate has a slightly different formula = diagonal conjugate - 1 to 2.6 cm.Conclusions: Several traits in sexual dimorphic of pelvic bone in Javanese do not match with skeletal traits widely used in Western literature, portraying the importance of studying regional human biological variation. The obstetric conjugate of Javanese pelves is not entirely the same with Western formula, though the difference is not significant.Key words: Javanese, pelves, sexual dimorphism, obstetric conjugate
oai:jurnal.ugm.ac.id:article/3039
2015-11-18T02:06:13Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3039
2015-11-18T02:06:13Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 01 (2007)
Mutagenicity Activity of Jatropha Curcas L. (Euphorbiaceae) Latex
I.Kristiani, Fazwishni Siregar
2015-11-18 09:06:13
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3039
id
Background: Folklore uses of Jatropha curcas L. latex are to cure toothache, as a mouth rinse to treat bleeding gums, as a hemostatic and a wound dressing and many others. Even though the latex has been used traditionally as a plant medicament, scientific investigation including toxicological studies was very limitedObjective: The purpose of this study was to evaluate the mutagenicity of J. curcas latex by Ames method. Materials and Methods: Five increasing concentrations of diluted latex (312-5000 !-tg) were mixed with phosphate buffer or 59 mix and cultured bacteria, incubated, and then added to top agar solution. The mixture was poured into a Petri dish on top of minimal glucose agar. After incubation for 2 days, the reverted coloni were counted. If the number of revertant increases with the dose and the number are more then twice as many as that of spontaneous revertant per plate, the compound should be judged to be positive.Result: Results showed that there were no increased numbers of colonies treated with increasing concentration of J. curcas latex. This finding is observed in all bacteria used. Conclusion: J. curcas latex is not mutagenic assessed by Ames method.Key words: Jatropha curcas latex, mutagenicity test, Ames test, traditional medicament, toxicology.
oai:jurnal.ugm.ac.id:article/3040
2015-11-18T02:06:13Z
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https://journal.ugm.ac.id/bik/article/view/3040
2015-11-18T02:06:13Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 01 (2007)
Comparison of the Diagnostic Test of Leukocyte Count, Percentage of Neurtrophyl, and C-Reactive protein (CRP) in Adult Patients With Simple and Complicated Acute Appendicitis
https://journal.ugm.ac.id/bik/article/download/3040/0
Ishandono Dachlan, Imam Sofii Agus Barmawi
2015-11-18 09:06:13
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3040
id
Background: acute appendicitis is one of the acute abdomen conditions that needs an emergency surgical procedure to prevent severe complication. If the perforation is present, the complications are general peritonitis, abscess and postoperative complication such as fistula and operative wound infection. Late diagnosis can increase the mortality and morbidity. There are about 11.2%-30% cases with intestinal perforation caused by late diagnosis. The leukocyte count, percentage of neutrophyl and C-reactive protein (CRP) are valuable informations to diagnose the perforation and nonperforation acute appendicitis in adult patients.Method: a prospective cross sectional study was held on diagnostic test from patient's database for acute appendicitis cases in Digestive Surgery Subdivision of Dr Sardjito Hospital from December 2005 until October 2006. The data was classified as simple and complicated acute appendicitis based on the histopathology result. Eighty two were found for both groups which fulfilled the inclusion and exclusion criteria. The diagnostic test Le. sensitivity, specificity, Positive Predictive Value (PPV), Negative Predictive Value (NPV), Likelihood Ratio (LR +), Likelihood Ratio (LR-I, accuracy and receiver operating characteristics (ROC) with area under the curve (AUC) were analyzed for the leukocyte count, neutrophyl, and C-reactive protein (CRP) in each group with histopathology result as a gold standard.Result: the leukocyte count, neutrophyl and C-reactive protein (CRP) had respectively sensitivity of: 70,07%; 74,54%; 89,09%; specificity 70,37%; 70,37%; 81,48%; Positive Predictive Value (PPV): 82,97%; 83,67%; 90,74%; Negative Predictive Value (NPV) 54,28%; 57,57%; 78,57%; Likelihood Ratio (LR+) 2,36%; 2,51 %; 4,56%, Likelihood Ratio (LR-) 0,42%; 0,22%; 0,13%, area under the curve (AUC) 0,797; 0,744; 0,891, and accuracy 70,73%; 73,17%, 86,56%.Conclusion: C-reactive protein (CRP) was a better diagnostic test to differ simple from complicated acute appendicitis in adult patient compared to leukocyte count and neutrophy!.Key words: simple and complicated acute appendicitis, leukocyte cout, neutrophyl, C- reactive protein
oai:jurnal.ugm.ac.id:article/3041
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https://journal.ugm.ac.id/bik/article/view/3041
2015-11-18T02:06:13Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 01 (2007)
Body Constitution, grip stregth, and Vital Capacity of Children 15 to 18 Years in Samigaluh Highland and Galur Lowland Kulonprogo Yogyakarta
Janatin Hastuti, Janatin Hastuti
2015-11-18 09:06:13
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3041
id
Background: Growth of children at age of 15 to 18 years old has an important influence on providing body constitution in their adult. Grip strength and vital capacity express functional effort of organs or parts of the body. Therefore, it certainly relates to the shape and size of the body or the body constitution. Different environment in highland and lowland affects the body constitution and functional capacity in growth period.ObJective: The aims of this research were to know the differences of body constitution, grip strength, and vital capacity of 15-18 year old children in Samigaluh highland and Galur lowland in Kulonprogo, Yogyakarta Province, and the relationship between body constitution, grip strength, and vital capacity of both populations. Methods: The research was done on 422 boys and girls of 1 5-18 years, which consisted of 152 children living in Samigaluh and 270 children living in Galur Kulonprogo. The children were examined with respect to body weight, height, grip strength of right and left hands, and vital capacity. Body Mass was Index then calculated from weight and height. Statistical analysis of three way ANOV A, t-test, Pearson product moment correlation, and linear regression were used.Results: Body constitution, grip strength, and vital capacity of boys differed significantly from girls. Boys were heavier and taller than girls. Grip strength and vital capacity were also greater in boys. Children living in Galur were heavier and taller than those living in Samigaluh, but they were similar in vital capacity. Grip strength was different at age of 15-16 years. There was no difference in Body Mass Index between boys and girls, as well as between Samigaluh children and Galur children.Conclusions: Body constitution, grip strength, and vital capacity of boys and girls were different significantly, also between Samigaluh and Galur children as well. Concerning Samigaluh children were shorter and lighter, it was said that Samigaluh children have better physical performance. Grip strength and vital capacity of 1 5 to 1 8 year old children living in Samigaluh highland and Galur lowland were stronger affected by body constitution than by age.Key words: body constitution; grip strength; vital capacity; 1 5-18 year old boys and girls
oai:jurnal.ugm.ac.id:article/3042
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https://journal.ugm.ac.id/bik/article/view/3042
2015-11-18T02:06:13Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 01 (2007)
The effect of fructooligosaccharide (FOS) in Children With Diarrhea
https://journal.ugm.ac.id/bik/article/download/3042/8446
Yati Soenarto, Mohammad Juffrie Yunri Istari, Nenny Sri Mulyani
2015-11-18 09:06:13
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3042
id
Latar belakang: Penyakit diare masih merupakan penyebab penting kesakitan dan kematian bayi dan anak di negara berkembang. Penyakit diare ini disebabkan oleh ketidakseimbangan flora usus yang dipengaruhi oleh jumlah bakteria yang menguntungkan . FOS dapat menstimulasi pertumbuhan bakteri yang menguntungkna dan menghambat bakteri pastogen yang menyebabkan infeksi saluran cerna Tujuan: Mengetahui efekFructooligosaccharide pada diareBahan dan cara: penelitina kendali acak dilakukan di 8 puskesmas di Yogyakarta. Seratus delapan belas sampel ikut dalam penelitian ini, 93 masuk dalam kelompok FOS yang mendapat FOS 2,5 gram atau 5 gram per hari sesuai umur, dan 25 masuk dalam kelompok plasebo. Lamanya diare dan keasaman tinja diukur selama penelitianHasil: Lama diare lebih pendek secara bermakna pada kelompok FOS dibanding plasebo p
oai:jurnal.ugm.ac.id:article/3044
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2015-11-18T02:06:13Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 39, No 01 (2007)
Antiplasmodial Activity of Fractions Isolated from Methanolic Extrac of Meniran Herb (Phyllanthus niruri L) traditionally Used to Treat Malaria
https://journal.ugm.ac.id/bik/article/download/3044/8447
Subagus Wahyuono, Mustofa Esti Nurwening Sholikhah
2015-11-18 09:06:13
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3044
id
Latar belakang: Tanaman meniran (P.niruri L.) secara tradisional telah digunakan untuk mengobati malaria. Penelitian pendahuluan terhadap berbagai ekstrak tanaman meniran menunjukkan bahwa ekstrak metanol adalah aktif terhadap P.falciparum. Namun demikian, fraksi manakah dalam ekstrak metanol yang mempunyai aktivitas antiplasmodium belum pernah dikaji.Tujuan: untuk mengetahui aktivitas antiplasmodium fraksi aktif tanaman meniran.Bahan dan cara: Fraksinasi yang mengacu pada bioassay dilakukan dengan menggunakan pelarut yang sesuai untuk memisahkan fraksi aktif dari ekstrak metanol. Pada awalnya, ekstrak metanol dipisahkan dengan etil asetat sehingga diperoleh fraksi larut etil asetat dan tidak larut etil asetat. Fraksi tidak larut etil asetat selanjutnya dipisahkan dengan kolom kromatografi dengan fase diam Sephadex LH-20 dan fase gerak n-hexan : metanol : asam asetat (7,5 : 12,5 v/v : 10 tetes) sehingga diperoleh 4 fraksi untuk diuji aktivitasnya. Aktivitas antiplasmodium pada strain P. falciparum ditunjukkan dengan nilai IC50 yaitu kadar fraksi aktif yang mampu menghambat pertumbuhan parasit hingga 50%. Identifikasi kandungan senyawa dalam fraksi aktif dilakukan dengan metode kromatografi lapis tipis (KLT).Hasil:Hasil penelitian menunjukkan bahwa fraksi tidak larut etil asetat leih aktif (IC50, 2,2-2,4 mg/mL) dari pada fraksi larut etil asetat (IC50, 4,3-4,8mg/mL). Dan pemisahan fraksi tidak larut etil asetat diperoleh 9 fraksi yang dapat dikelompokkan menjadi 4 fraksi berdasarkan gambaran KLTnya. fraksi III (FIII) merupakan fraksi paling aktif dengan IC50, 3,4-4,1 mg/mL. Identifikasi kandungan aktif F III dengan KLT menunjukkan adanya senyawa polifenol. Penelitian lanjut akan dilakukan untuk mengisolasi dan memurnikan senyawa polifenol dan mengkaji aktivitas antiplasmodiumnya.Simpulan: Fraksi III dari ekstrak metanol meniran yang mengandung senyawa polifenol merupakan fraksi paling aktif secara in vitro terhadap P.falciparum.Key words: Phyllanthus niruri, malaria, antiplasmodial activity, in vitro, polyphenol
oai:jurnal.ugm.ac.id:article/3184
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https://journal.ugm.ac.id/bik/article/view/3184
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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 02 (2012)
Onset of menarche: differences between urban and rural community in Tangerang District, Banten Province, Indonesia
Suryono Yudha Patria, Dewi Darmawati Sunartini Hapsara
2015-11-18 09:03:49
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3184
id
The transition of physical and sexual maturation in becoming an adult occurs in adolescenceperiod. This transition runs in parallel with the development of cognitive, emotional, socialcultural and adaptation ability. Girls sexual maturity is marked by first menstruation (menarche)which is the most important phase of reproductive development. The relationship betweenmenarche and growth rate of body height is very close. The menarche occurs when the growthrate of the body height begins to decline. Many factors influence the onset of menarche i.e.physical activity, nutritional intake, socio economic and environmental conditions and availabilityof medical services. These factors vary between rural and urban area. Girls in urban area tend tohave modest life style compared with their counterpart in rural area. The purpose of this studywas to compare the onset of menarche of girls in urban and rural area, and to identify factorsthat influence the onset of menarche. This was a cross sectional study involving 220 girlsstudents of elementary school from both urban and rural area in Tangerang District, BantenProvince. The mean onset of menarche of all subjects was 11.46 ± 0.99 years, while the meanonset of menarche of girls who live in rural area (11.87±0.99 years) was significantly slowerthan girls who live in urban area (11.05±0.80 years) (p=0.01). The birth body weight and birthbody length, physical activity and environment condition significantly influenced the onset ofmenarche. The girls who live in urban areas had an earlier predictor factors to achieve for onsetof menarche than those who live in rural areas (OR: 3.34 [95% CI: 1.911 to 5.823] and aOR:2.95 [95% CI: 1.410 - 6.012]).Keywords: menarche - growth spurt - sexual maturity - urban - rural community
oai:jurnal.ugm.ac.id:article/3185
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https://journal.ugm.ac.id/bik/article/view/3185
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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 02 (2012)
Efficacy of ketamine gargle 40 mg and benzydamine HCl 0.075% in reducing post intubation sore throat
Djayanti Sari, Eko Bambang Subekti MuhdarAbubakar
2015-11-18 09:03:49
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3185
id
Sore throat is a frequent complication in postoperative patients under general anesthesia whichis performed with endotracheal tube intubation. This effect occurs because of mucosal irritationand damage and airways inflammation due to a lubricant gel on laryngoscopy or an emphasis ofendotracheal tube cuff. This study was conducted to compare the efficacy of ketamine 40 mggargle with benzydamine HCl 0.075% gargle in reducing post intubation of sore throat prior tothe insertion of endotracheal tube. A prospective randomized double blind controlled clinicaltrial enrolled patients aged 18-50 years with physical status of ASA I and II whounderwent elective surgery with general anesthesia in Dr. Sardjito General Hospital, Yogyakarta.Subjects were divided into 2 groups. 50 patients of group A were given 30 mL ketamine gargle40 mg and 51 patients of group B were given 30mL benzydamine HCl gargle 0.075%. The sorethroat was measured whenever the patients were fully awake, 2 hours and 4 hours post-extubationwith Canbay score (no pain, mild pain, moderate pain and severe pain). There was a significantdifference (p<0.01) in sore throat incidence of both groups especially at fully awake and 2hours post-extubation; 20% in group A versus 66.7% in group B at fully awake and 16% ingroup A versus 58,8% in group B at 2 hours of post-extubation. However, there was not anysignificant difference in sore throat incidence of both groups at 4 hours post-extubation(p=0.394). In conclusion, giving ketamine gargle 40 mg 5 minutes before intubation is moreefficient in reducing sore throat after endotracheal tube-general anesthesia compared withbenzydamine HCl 0.075% at fully awake and 2 hours post-extubation.Keywords : endotracheal tube - sore throat - ketamine gargle - benzydamine HCl gargle
oai:jurnal.ugm.ac.id:article/3186
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https://journal.ugm.ac.id/bik/article/view/3186
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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 02 (2012)
Polymorphism of vascular endhothelial growth factor (VEGF) gene insertion/ deletion -2549 as risk factor of diabetic retinophathy in Javanese patients with type 2 diabetes
Maliyah Madiyan, Jems Kifen Roget Maay Ahmad Hamim Sadewa
2015-11-18 09:03:49
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3186
id
Diabetic retinopathy (DR) is a visual disorder caused by the diabetic microvascular complications.Genetic polymorphism in the vascular endothelial growth factor (VEGF) gene plays an importanrole in the susceptibility of DR. The aim of this study was to evaluate the association of thepolymorphism of VEGF gene insetion/deletion (I/D) -2549 with DR in Javanese type 2 diabetesmellitus (DM) patients. This was a case control study involving 40 Javanese type 2 DM patientswith DR as case subjects and 40 Javanese type 2 DM patients without DR as control subjects.Type 2 DM patients with DR were recruited from Eye Polyclinic, whereas type 2 DM patientswithout DR were recruited from Endocrine Polyclinic of Dr. Sardjito General Hospital, Yogyakarta.Genotyping of VEGF gene I/D-2549 was conducted using PCR-RFLP method. Plasma VEGFlevels were measured using Enzym-Linked Immunosorbent Assay (ELISA). The genotype distributionof DD (67.5%) and the allele frequency of D (82.5%) in type 2 DM patients with DR was higherthan those without DR (27.5% for DD genotype and 56.3% for D allele). The OR of DD and IDgenotypes versus II genotype between type 2 DM patients with DR and without DR was 6.882(95%CI: 0.789-60.060; p=0.048), whereas OR for the D allele versus I allele between type 2 DMpatients with DR and without DR was 3.667 (95%CI: 1.773-3.667; p=0.000). The plasma VEGFlevels of DD genotype (92.16±49.73 pg/mL) were significantly higher than ID genotype(42.70±33.29 pg/mL) in type 2 DM patients (p=0.000). In conclusion, the polymorphism ofVEGF gene I/D -2549 is associated with DR in Javanese type 2 DM patients. The DD genotypeand D allele of the VEGF gene polymorphism are the risk factor of DR in those patients. Theassociation of the polymorphism of VEGF gene with DR may be explained with the high plasmaVEGF level.Keywords: vascular endothelial growth factor (VEGF) - polymorphism - diabetes - retinopathy- Javanese patients
oai:jurnal.ugm.ac.id:article/3187
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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 02 (2012)
Assessment of maximal urinary flow rate (Qmax) of urethral stricture patients three weeks post internal urethrotomy Sachse in Dr. Sardjito General Hospital Yogyakarta
Ishandono Dachlan, Juni Ariston Tambunan Prawito Singodimedjo
2015-11-18 09:03:49
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3187
id
Urethral stricture is a common urologic problem in developing countries including Indonesia dueto its high prevalence. Internal urethrotomy is still the gold standard to return patients to a stateof normal voiding. To evaluate the outcome of the internal urethrotomy, uroflowmetry assessmentcan be conducted with its principal variable of maximal urinary flow rate (Qmax). Since 1985, inDr. Sardjito General Hospital Yogyakarta, the internal urethrotomy has been used as the maintreatment modality to manage the urethral stricture. However, its outcome has not beenevaluated. The aim of this study was to evaluate Qmax of urethral stricture patients postinternal urethrotomy Sachse in Dr. Sardjito General Hospital. This was a cross-sectional studyperformed starting from November 2009 to April 2010. The Qmax was assessed using theuroflowmeter three weeks after internal urethrotomy. The length and the locations of the patients’stricture, as well as its correlation with Qmax were also measured and evaluated. Among 24patients selected, 13 patients who fulfilled the inclusion and exclusion criteria were involved inthis study. The mean of the Qmax of patients was 22.3±6.7 mL/s.The mean of Qmax ofpatients who had the length of urethral stricture of d” 2 cm (14.8±3.8 mL/s) was significantlyhigher than patients who had length of à 2 cm (6.4±2.6 mL/s) (p=0.03), whereas patients whohad the location of urethral stricture on anterior (12.4±5.4 mL/s) were not significantly differentcompared to patients who had those on posterior (8.5±4.9 mL/s) (p=0.398). In conclusion, themajority of patients returned to a state of normal urinary tract function post internal urethrotomy.The Qmax of urethral stricture patients after internal urethrotomy are influenced by the lengthof the stricture but not by its location.Keywords: urethral stricture - urethrography - Sachse - uroflowmetry - Qmax
oai:jurnal.ugm.ac.id:article/3188
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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 02 (2012)
Clinical signs as diagnostic test to assess hypoxemia in children with acute asthma exacerbation
Endy Paryanto Prawirohartono, Latifah Hanum Roni Naning
2015-11-18 09:03:49
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3188
id
Asthma attack can cause hypoxemia. One of the methods to detect hypoxemia is by using pulseoximetry. However, this tool is not always available in some health care centres. Therefore, amore rapid and simple diagnostic tool is needed as an alternative method to detect hypoxemia.This study aimed to assess signs and symptoms as diagnostic tools for hypoxemia in childrenwith asthma. This was an analytical observational with cross-sectional design performed inDepartment of Pediatrics, Dr. Sardjito General Hospital/Faculty of Medicine, Universitas GadjahMada, Yogyakarta. The study was started in Februari 2010 until the sample size was sufficient.Seventy seven children with asthma between 0 – 18 years old who were presented to EmergencyDepartment and Respiratory Outpatient Clinic were involvoled in this study. All subjects wereexamined for clinical signs and oxygen saturation as the gold standard. The prevalence ofhypoxemia in children with asthma in this study was 18.2%. The best single clinical predictor ofhypoxemia was tachycardia that yielded a sensitivity of 86% (95%CI: 67 – 100%) and specificityof 59% (95%CI: 49 – 71%), and nasal flaring yielded a sensitivity of 79% and specificity of79%. The combination of 2 clinical signs namely chest wall retraction-nasal flaring increased asensitivity of 79% and specificity of 71%, chest wall retraction-tachycardia increased a sensitivityof 86% and a specificity of 76%, chest wall retraction-tachypnoe increased a sensitivity of 86%and a specificity of 51%, tachycardia-tachypnoea increased a sensitivity of 79% and a specificityof 76%. The combination 3 clinical sign namely chest wall retraction-tachycardia-tachypnoeayielded a sensitivity of 79% and specificity of 79%. In conclusion, chest wall retraction andtachycardia have higher diagnostic score than other clinical signs to assess hypoxemia in childrenwith asthma on acute exacerbation.Keywords: asthma - hypoxemia - clinical signs - children - assessment
oai:jurnal.ugm.ac.id:article/3189
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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 02 (2012)
Multiple mini punch grafts for extensive ulcer: a case report
Wirohadidjojo, Yohanes Widodo
2015-11-18 09:03:49
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3189
id
Multiple mini punch grafts is the placing of mini size of full thickness skins on to ulcer bed. Theyconsist of epidermal and dermal component composed with hair follicles and other skin appendiceswhere epidermal stem cells are located. The epidermal stem cells are the best source of epidermalcells in reconstruction of skin equivalent that is usually used for replacing classic split thicknessskin graft in recovering extensive ulcer. In this article, the application of multiple mini punchgrafts onto extensive ulcer is reported. A case of extensive ulcer was suffered by a 6-year-oldboy whose left foot is injured in a traffic accident. His toes had already been amputated bysurgeon but a classic skin graft failed to recover the ulcer. Multiple mini punch grafts had beenharvested from his inguinal and buttock skin and they were placed onto his ulcer. Pre and postmini punch grafting photographs were reviewed. After eight weeks, placed multiple mini punchtissues onto large ulcer reveals lateral extensions and more than 90% of epithelialization. Multiplemini punch grafts can be used as a method to cover large ulcer.Key words: mini punch grafts-large ulcer-epithelialization-epidermal-stem cells
oai:jurnal.ugm.ac.id:article/3191
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https://journal.ugm.ac.id/bik/article/view/3191
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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 02 (2012)
The effect of food supplementation on nutritional status of severemalnourishment children aged 12-59 months in Sleman District, Yogyakarta Special Region
Soeroyo Machfudz, Ahmad Lubaid Endy Paryanto Prawirohartono
2015-11-18 09:03:49
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3191
id
Malnutrition and malnourishment are still big problems among children in Indonesia. Age between12 to 59 months is the most important and critical time periods for the physical and intelligencedevelopment of children. Therefore, the children should obtain a good health care and nutritionaccording to their needs. The purpose of this study was to evaluate the influence of foodsupplementation on the nutritional status of children in Sleman District, Yogyakarta SpecialRegion. This was a quasi experimental study involving 60 severe malnourishment children betweenthe age of 12-59 months from Sayegan Sub Districts. Body weight and height of the childrenwere measured before and after food supplementation. Food supplements were given for 100days. The outcome of this study was the change of nutritional status based on Z-score accordingto the Ministry of Health classification adopted from WHO-NCHS (World Health Organization-US National Center for Health Statistics) recommendations. According to Z score for weight-forage(WAZ), the food supplementation did not improve the nutritional status of children. Althoughthe nutritional status of 25.87% children increased and the mean of WAZ significantly increased1.04 to be -3.29 after supplementation (p<0.05), the mean of nutritional status of the childrenwas still the worst (WAZ <-3SD). According to Z score height-for-age (HAZ), foodsupplementation did not improve nutritional status of the children either. The mean of HAZbefore food supplementation (-2.53) was not signifantly different compared to after foodsupplementation (-2.45). Moreover, mean of nutritional status of the children after foodsupplementation was still short (HAZ <-2SD). According to Z score weight-for-height (WHZ),food supplementation improved the nutritional status in 86.21% children. The mean of WHZsignificantly increased 1.13 from -3.35 to be -2.22 after food supplementation (p<0.05) indicatingthe change of nutritional status from wasted (WHZ <-3SD) to thin (WHZ <-2SD to -3SD). Inconclusion, food supplementation on children aged 12-59 months succeeds to improve theirnutritional status according to WHZ.Keywords: poor family - nutritional status - children - severe malnourishment - Z-score
oai:jurnal.ugm.ac.id:article/3192
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v2
https://journal.ugm.ac.id/bik/article/view/3192
2015-11-18T02:03:49Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 02 (2012)
Expression of receptor advanced glycation end products (RAGE) and histological picture of pancreatic -cells of streptozotocin-induced diabetic rats after yellow soybean powder suspension (Glycine max) administration
Sri Kadarsih Soejono, Dominggos Gonsalves Sri Lestari Sulistyo
2015-11-18 09:03:49
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3192
id
Diabetes mellitus is a multisystemic disease characterized by hyperglycemia due to defects ininsulin secretion and action, or both. Hyperglycemia is the most important factor in the onsetand progress of diabetic complications. Hyperglycemia increases the expression of receptor foradvanced glycosylation end products (RAGE) which leads to pancreatic -cells damage. Yellowsoybean (Glycine max) is reported to contain isoflavones which have various biological propertiesincluding antidiabetes. Dietary soybean can prevent the progression of diabetic complications.This study was conducted to investigate the effects of yellow soybean powder suspension onexpression of RAGE and pancreatic -cells damage of diabetic rats. Thirty streptozotocin (STZ)-induced diabetic Sprague Dawley male rats aged 11-12 weeks with body weight 200-250 g wereused in this study. The rats were divided into 5 groups with 6 rats in each group. Group 1 wasnon diabetic rats. Group 2 was diabetic rats without treatment. Group 3-5 were given yellowsoybean powder suspension of 400, 800 and 1600 mg/kg BW for four weeks, respectively. Atthe end of the experiment, pancreases tissues were removed for examination of RAGE expressionand pancreatic -cells. The results showed that yellow soybean powder suspension ingestionsignifantly decreased blood glucose level of diabetic rats toward normality (p<0.05). Moreover,the percentage of RAGE expression on Group 3 (50.01±2.75%) and Group 2 (53.03±4.02%)were not significantly different (p>0.05). Meanwhile, the percentage of RAGE expression onGroup 4 (42.43±4.08%) and Group 5 (40.62±3.42%) were significantly lower than Group 2(p<0.05). The percentage of pancreatic -cells on Group 2 (10.04±1.56%) was not significantlydifferent compared to Group 3 (8.61±0.81%) (p>0.05), whereas the percentage of pancreatic-cells in Group 4 (16.78±7.79%) and in Group 5 (22.03±11.51%) were significantly higherthan Group 2 (p<0.05). In conclusion, yellow soybean powder suspension can decrease RAGEexpression and prevent pancreatic -cells damage on STZ-induced diabetic rats.Keywords: pancreatic cells - Langerhans islets - streptozotocine - diabetic rats - soybeans
oai:jurnal.ugm.ac.id:article/3193
2015-11-18T02:03:49Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3193
2015-11-18T02:03:49Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 02 (2012)
The effect of a formulation containing honey, black cumin, propolis and royal jelly on blood glucose level and pancreatic -cells of streptozotocin-induced diabetic rats
Setyo Purwono,Sitarina Widyarini,Mustofa, Sunyoto, Umi Mahmudah, Sindu Oktivasari,Eti Nurwening Sholikhah
2015-11-18 09:03:49
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3193
id
A formulation containing 40% of honey, 30% of black cumin, 20% of propolis and 10% of royaljelly has been available in the market for the treatment of diabetes. Although each content ofthe formulation is proven to possess antioxidant and antihyperglycemic activities, its combinationeffect has not been evaluated yet. The aim of this study was to evaluate the effect of thisformulation on blood glucose level and pancreatic -cells of streptozotocin (STZ)-induced diabeticrats. Thirty six male Wistar rats (Rattus novergicus) aged 11 weeks with body weight 100-150 gwere used in this study. The rats were divided into 6 groups with 6 rats in each group. Group 1was non diabetic rats that were given aquadest. Group 2 was diabetic rats that were givenaquadest. Group 3 was diabetic rats that were given metformin at dose of 45 mg/kg BW. Group4-6 were diabetic rats that were given formulation tested at dose of 3, 6 and 12 mL/kg BW,respectively. All rats were induced by intraperitoneal injection of STZ at 60 mg/kg BW anddiabetic rats were then orally administered the formulation tested or metformin twice daily for14 days. Blood glucose level was monitored on day 10 and 17 after STZ induction. Rats weresacrificed and pancreas samples were taken for histopathological examination. The results showedthat the blood glucose level decreased significantly after seven days of treatment with metforminor fomulation tested and continued after 14 days of treatment. The blood glucose level ofdiabetic rats after 14 days of treatment returned to the normal level. The vacuolization of thepancreatic -cells of diabetic rats treated with metformin or with formulation tested were lowerthan untreated diabetic rats but still higher than non diabetic rats. In conclusion, the formulationtested has antihyperglicemic and protective effect on -cells damage in diabetic rats.Keywords: diabetic rats - honey - black cumin - propolis - royal jelly - antihyperglicemic
oai:jurnal.ugm.ac.id:article/3194
2015-11-18T02:03:49Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3194
2015-11-18T02:03:49Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 02 (2012)
Candida albicans biofilm: formation and antifungal agents resistance
Wibawa, Tri
2015-11-18 09:03:49
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3194
id
Candida sp are the most common fungal pathogens causing fatal health care associated infections.Among the genus of Candida, Candida albicans is the most frequent species isolated frompatients. The notorious C. albicans infection is the ability of this dimorphic fungus to formbiofilm. Biofilm has been pointed as a dynamic phenotypic switching in bacteria and fungi,which may result in higher morbidity and mortality in human beings. This review addresses thebasic explanation of biofilm formation which is characterized by the antifungal agents resistance.The factors that influence C. albicans biofim formation and antifungal agents resistance arediscussed.Key words: Candida sp – antifungal – resistance – biofilm - pathogenecity
oai:jurnal.ugm.ac.id:article/3253
2016-03-28T03:56:25Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3253
2016-03-28T03:56:25Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 47, No 2 (2015)
The effect of proton pump inhibitors and clopidogrel combination to the risk of recurrent stroke
Ngatidjan, Endang Mahati Jarir Atthobari
2016-03-28 10:38:04
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3253
Recurrent stroke is very common among stroke survivors. Clopidogrel is one of the drugs used in the prevention of recurrent stroke. Because clopidogrel may cause gastrointestinal bleeding, the use of proton pump inhibitors may elicite the side effect, but unfortunately this combination may interfere the antiplatelete activity of clopidogrel since both are metabolized by the same enzyme, liver CYP2C19. The aim of this study was to know the effect of proton pump inhibitors on the efficacy of clopidogrel in the prevention of recurrent stroke in Bethesda Hospital, Yogyakarta. This study was a case control study. The sample size of treatment case was 392 patients and control case was 784 patients. The case group was patients with recurrent stroke and the control group was the patients who come to outpatient clinics of Department of Neurology. In this study, matching was made for treatment and control groups on sex, age and their index date. The exposure of the combination was investigated in the last 6 months and categorized as current use (<2 month before index date) and recent use (2-6month before index date). Multivariate analysis showed that there was no association between clopidogrel and proton pump inhibitors to the risk of recurrent stroke OR 1.05 (95% CI 0.56-1.785). Omeprazole was the most used, although there was no significantly increased risk OR 0.46 (95% CI 0.56-3.70). The effect of proton pump inhibitors to the efficacy of clopidogrel has not been known yet in this study, because the amount of total sample that used the combination of this drug was too small.Keywords: clopidogrel - proton pump inhibitors - recurrent stroke - interaction
oai:jurnal.ugm.ac.id:article/3266
2015-11-18T02:03:56Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3266
2015-11-18T02:03:56Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 01 (2012)
Neuroprotective effect of vitamin D3 toward apoptosis induced by ethanol in CA1 pyramidal cells of rat hippocampus
Dwi Cahyani Ratna Sari, Junaedy Yunus Djoko Prakosa
2015-11-18 09:03:56
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3266
As an antioxidant, Vitamin D3 can protect neurons from damage caused by oxidative stress.Ethanol is known to have neurotoxic effects by inducing an increase in oxidative stress. One ofthe brain regions that is most sensitive to neurotoxic effects induced by ethanol is hippocampus,especially its CA1 region. This study was aimed to determine the neuroprotective effects ofvitamin D3 in preventing the apoptosis in CA1 hippocampal pyramidal cells induced by ethanol.Fifteen male Wistar rats (Rattus norvegicus) were randomly divided into three groups. The controlgroup was given daily normal saline solution intraperitoneally. The ethanol group was given20% ethanol solution at a dose of 3 g/kg BW/day intraperitoneally. The vitamin D3 group wasgiven vitamin D3 1 μg/kg BW/day in 20% ethanol solution at a dose of 3 g/kg BW/dayintraperitoneally. After 30 days, the rats were sacrificed, their brains were perfused with PBSfollowed by fixative and the hippocampus was dissected for histological preparations.Immunohistochemical staining for caspase was performed. Percentage of apoptotic CA1hippocampal pyramidal cells was calculated. The results showed there was no significant difference(p> 0.05) on the total number of pyramidal cells between the control group (20.52 ± 1.31), theethanol group (19.02 ± 1.60), and the vitamin D3 group (21. 06 ± 0.70) per field of view.However there was a significant increase (p<0.05) in the percentage of apoptotic CA1hippocampal pyramidal cells in in the ethanol group (16.09 ± 0.67%) compared to the controlgroup (10.60 ± 0.95%). Vitamin D3 significantly (p<0.05) prevented an increase in the percentageof apoptotic CA1 hippocampal pyramidal cells in the vitamin D3 group (10.82 ± 0.64%). Inconclusion, vitamin D3 had a neuroprotective effect to prevent an increase in apoptosis in CA1hippocampal pyramidal cells to the neurotoxic effects induced by ethanol.
oai:jurnal.ugm.ac.id:article/3267
2015-11-18T02:03:56Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3267
2015-11-18T02:03:56Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 01 (2012)
Activity of -terpineol as a potential anticancer candidate: cytotoxicity, proapoptotic and antiproliferative evaluation in TD47 cell lines
Mustofa, Puguh Indrasetiawan Indwiani Astuti
2015-11-18 09:03:56
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3267
Previous study has shown that -terpineol has a cytotoxic effect on NCI-H69 cancer cell line.This study aimed to evaluate cytotoxicity, proapoptotic, and antiproliferative activities of -terpineol in T47D cell lines. The cytotoxic activity of -terpineol was determined using MTT cellviability assay, while apoptosis was tested by the acridine orange-ethidium bromide stainingmethod. The cell cycle was evaluated by flowcytometry method. The results showed that -terpineol had cytotoxic effect on the T47D cells with the IC50 value of 135.00 ± 8.74 M.Furthermore, -terpineol induced the cells apoptosis in a dose-dependent manner. Flowcytometryanalysis showed that -terpineol induced the T47D cell distribution in Go/G1 phase, howeverinhibited cell distribution in S phase. This study suggests that -terpineol has a potential anticanceractivity.
oai:jurnal.ugm.ac.id:article/3268
2015-11-18T02:03:56Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3268
2015-11-18T02:03:56Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 01 (2012)
Serum testosterone level and active caspase-3 of Leydig cells of diabetic Sprague-Dawley male rats after administration of soybean (Glycin max) powder suspension
Sri Kadarsih Soejono, Wiyadi Dicky Moch. Rizal
2015-11-18 09:03:56
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3268
Diabetes mellitus (DM) is known as the main death cause in the world. This disease causes acuteand chronic complication. The common chronic complication on male reproduction system isthe decrease of testosterone level. This hormone is produced in the Leydig cells. Soybeans(Glycin max) has been used in the management of DM to maintain blood glucose level. However,the effect of soybean on serum testosterone level is still unclear. The aim of this study was toevaluate serum testosterone level and caspase-3 active of Leydig cells of diabetic Sprague-Dawley (SD) male rats model induced by streptozotocine (STZ) after oral ingestion of soybeanpowder suspension. This was an experimental study with pre and post test control group design.Thirty SD male rats, aged 11-12 weeks with body weight (BW) of 200-250 g were divided into 5groups with 6 rats in each group. The first group (G1) was normal rat control and the secondgroup (G2) was diabetic rat control. The third to fifth group (G3, G4 and G5) were diabetic rattreatment with oral ingestion of soybean powder suspension that was given once a day during 4weeks with dose of 400; 800 and 1600 mg/kg BW, respectively. The serum testosterone levelwas measured by an ELISA and active caspase-3 of Leydig cells were measured by animmunohistochemistry method. The result showed that the serum testosterone level and activecasapse-3 Leydig cells of diabetic rats model induced by STZ were not significantly differentafter oral ingestion of soybean powder suspension with dose of 400, 800 and 1600 mg/kg BW(p>0.05). In conclusion, soybean powder suspension did not affect the serum testosteronelevel and active caspase-3 Leydig cells in diabetic rats.Keywords: diabetes - soybean - phytoestrogen - testosterone - active caspase-3
oai:jurnal.ugm.ac.id:article/3269
2015-11-18T02:03:56Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3269
2015-11-18T02:03:56Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 01 (2012)
Comparison of postthawing sperm motility recovery between cryopreserved with and without cryoprotective agent using 4 different cryopreservationmethods
Irwan Taufiqur Rachman, Hilwah Nora Shofwal Widad
2015-11-18 09:03:56
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3269
Frozen-thawed human spermatozoa are routinely used for many assisted reproduction program.However, cryopreserved spermatozoa was reported to yield lower pregnancy rates compared tofresh semen in both intra uterine inseminations and in vitro fertilization/intracytoplasmic sperminjection (IVF/ICSI) due to the reduction of sperm motility and viability induced by cryopreservationprocedures. This study was aimed to evaluate the influence of cryoprotective agent (CPA) andcryopreservation methods on human sperm motility. This was a quasi experimental study. Thirtyseven normozoospermic semen samples collected in Permata Hati Infertility Clinics of Dr. SardjitoGeneral Hospital, Yogyakarta were recruited. Four different cryopreservation methods were appliedusing and without CPA (TEST-yolk buffer). In simple two steps freezing, cryostraw were graduallyfrozen from 8 to -4°C. In simple graduated freezing, cryostraus were directly frozen at -4°C. Invapor phase freezing method, the samples in cryostraw were placed 1 cm above liquid nitrogen.In the last method, the samples were directly submerged into liquid nitrogen. Thawing wasconducted by incubation at 37°C for 5 minutes. The sperm motility recovery after cryopreservationin the 4 different cryopreservation methods was evaluated and analyzed by analysis of variance(ANOVA). The fresh sperm motility before cryopreservation was 52.9 ± 4.50%. The recovery ofmotile sperms was 17.00 ± 7.83%, 20.96 ± 5.81%, 15.06 ± 8.55% and 15.68 ± 8.3%, whenusing CPA and 5.63 ± 4.63%, 5.47 ± 3.95%, 4.45 ± 4.46% and 6.08 ± 5.06% when withoutCPA following direct plunge to liquid nitrogen freezing, vapor liquid nitrogen freezing, simplegraduated freezing and simple 2-steps freezing, respectively. Among methods using CPA, thevapor phase method resulted in highest sperm motility recovery. In methods without CPA, nosignificant difference of sperm motility recovery was observed among the 4 differentcryopreservation methods. In conclusion, the use of CPA for cryopreservation improves spermmotility recovery.
oai:jurnal.ugm.ac.id:article/3270
2015-11-18T02:03:56Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3270
2015-11-18T02:03:56Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 01 (2012)
Effect of temperature and storage duration of Aedes aegypti mosquito specimens artificially infected with dengue-3 virus on the results of immunohistochemical examination
Tri Baskoro Tunggul Satoto, Susilawati Sitti Rohmah Umniyati
2015-11-18 09:03:56
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3270
To confirm the presence of any dengue viral in a mosquito, mosquito’s head can be squashed ona slide and stained with immunohistochemical staining. The remaining samples then can bestored in the cryo freezer at -80ºC to avoid specimen damage. However, for laboratories withlimited facilities, with only a refrigerator with a temperature range of -20ºC to 4ºC is available,examination to evaluate whether the dengue antigen can still be detected in specimens storedat these temperature is necessary. It was a quasi-experimental study. Three to five-day-old adultfemale Aedes aegypti mosquitoes were injected intrathoracically with dengue-3 (DENV-3) strainH-87 virus and then maintained for about 7 days. The dengue viral antigen on mosquitoes wasidentified using immunohistochemical method after stored at a temperature of 4oC, -20oC, -80oCfor 2, 4 and 8 weeks. Mosquito specimens that were not stored were used as a positive control.Kappa value was counted to analyze level of agreements between two observers. Two-wayAnova was used to analyze mean positive rates. Kappa value showed poor agreement (0.00-0.16) between two observers when the specimens were stored at 4oC for 2-8 weeks, and showedgood agreement (Kappa value of 0.77), when stored at -20oC for 4 weeks. The kappa valueshowed very good agreement (0.90-0.92) when the specimens were stored at -20oC for 2 weeks,and at -80oC for 2-8 weeks. Mean positive rates of the specimens stored at 4oC were significantlylower (p<0.005) than stored at -20oC and -80oC, but there were no significant differencesbetween specimens stored at -20oC and -80oC (p>0.05). In conclusion, availability of thedengue viral antigen on mosquito specimens was influenced by temperature and storage durationof the specimens.Keywords: dengue virus - head squash - immunohistochemistry - kappa agreement - temperature
oai:jurnal.ugm.ac.id:article/3271
2015-11-18T02:03:56Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3271
2015-11-18T02:03:56Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 01 (2012)
The correlation between TAM,MVD, VEGF andMMP-9 expressions among various histological progression, histological grading and staging of breast cancer
Angelina Septiana, Brian Arditya M, Irianiwati, Harijadi Surendra Prabawa, Yulius Hermanto
2015-11-18 09:03:56
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3271
Breast cancer may progress from an atypical lesion. Angiogenesis has an important role in thegrowth, invasion and metastasis of breast cancer, which can be reflected through the microvasculardensity (MVD). Tumor associated macrophages (TAMs) are stromal cells that can produce proangiogenicfactor such as vascular endothelial growth factor (VEGF), and induce matrixmetalloproteinase-9 (MMP-9) for degrading extracellular matrix and basement membrane.Angiogenesis in breast cancer progression and its relationship with histological grading as wellas its staging need to be defined and thus, cancer therapy and prognosis can be determinedmore accurately. The aim of this study was to investigate the correlation between TAM, MVD,VEGF and MMP-9 expressions among fibrocystic lesion, atypical lesion and breast cancer, andits correlation with histological grading and staging of breast cancer. Using a cross-sectionalstudy, a total of 50 paraffin embedded tissues of fibrocystic lesion, atypical lesion and breastcancer were chosen in this study. Those specimens were stained immunohistochemically withmonoclonal antibody (MoAb) anti CD68, von Willebrand factor (vWF), VEGF and MMP-9. Theexpressions of VEGF and MMP-9 were counted from the mean numbers of positive tumor cells.TAMs were counted from numbers of macrophages which expressed CD-68. MVDs were countedfrom numbers of microvessels whose endothelial cells expressed vWF, using the AverageMicrovessels Count ( AMC) method. The correlation of both markers and different type of breastlesions were analyzed by using Pearson correlation. There were statistically significant correlationsbetween TAM (r=0.760; p=0.000), MVD (r=0.659; p=0.000), and MMP-9 (r=0.518; p=0.000),among several breast lesions and histological grade of breast cancer. The highest of theirexpressions was found in the poor grade of cancers. There were statistically significant correlationsbetween TAM (r=0.581; p=0.000), VEGF (r=0.443; p=0.001) and MVD (r=0.566; p=0.000)among fibrocystic, atypical lesion and stage II - III of breast cancer. VEGF expression was notsignificantly correlated with several histological grade of breast cancer and the highest of itsexpression was only found in atypical lesion. This study has suggested that TAM, MVD, VEGFand MMP-9 expressions might play an important role in the histological progression, histologicalgrading and staging of breast cancer. The highest expression of VEGF in atypical breast lesionsupported the fact that angiogenic switch already started in the early stage and grade of breastcancer.
oai:jurnal.ugm.ac.id:article/3272
2015-11-18T02:03:56Z
bik:ART
v2
https://journal.ugm.ac.id/bik/article/view/3272
2015-11-18T02:03:56Z
Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Vol 44, No 01 (2012)
The droplet size changes of 1% propofol before and after the storage procedure for 6 and 24 hours periods
Djayanti Sari, Basuki Rahmat Bambang Suryono S
2015-11-18 09:03:56
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) by Universitas Gadjah Mada is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.
url:https://journal.ugm.ac.id/bik/article/view/3272
Propofol (2,6-diisopropylphenol) is a popular anesthetic agent for induction and anestheticmaintenance. Propofol preparation is formulated as a lipid macroemulsion that is thermodynamicallyunstable and degraded over time, causing the enlargement of droplet size. The degradation is fasterwhen propofol emulsion preparation package has been opened. This droplet enlargement results indecreasing propofol releasing-rate and its concentration. The risk of embolism occurs whenever themean droplet size (MDS) exceeds Food and Drug Administration (FDA) limit (<0.45) and fat globulepopulation percentage >5 m (PFAT5) is <0.05%. In the daily practice, some propofol emulsionpreparation are often used although they are already opened and saved more than 24 hours. The aimof this study was to evaluate the droplet size changes of propofol emulsion preparation duringstorage. This was an observational study using cohort prospective design. The droplet size changesof sixteen propofol preparation samples were evaluated before and after storage procedure for 6 and24 hours in the refrigerator at the temperature of 4ºC. The droplet diameter was determined under alight microscope. Mean droplet size before and after storage were calculated and analyzed by oneway analysis of variance (ANOVA), followed by Tukey’s post hoc test with 95% confidence interval(p<0.05). The droplet diameter of propofol emulsion preparation increased significantly (p<0.05)after storage procedure for 6 h (MDS value were: 247 ± 22 nm) and 24 h (278 ± 29 nm) comparedto before storage (225 ± 24 nm). The PFAT5 at each interval time was 0%. There was no color andhomogeneity changing at each interval time (n=16/100% each time interval). In conclusion, there isa change of propofol emulsion preparation droplet size after storage procedure for 6 to 24 hours attemperature of 4ºC compared to before storage.
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