2024-03-29T11:25:30Z
https://journal.ugm.ac.id/index/oai
oai:ojs.journal.ugm.ac.id:article/7744
2015-11-06T03:32:50Z
TradMedJ:ART
oai:jurnal.ugm.ac.id:article/7745
2015-11-18T03:23:01Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
ANALGESIC EFFECT OF COMBINATION OF ESSENTIAL OIL Curcuma xanthorriza Roxb. AND ITS CURCUMINOIDS IN MICE
Wicaksono, Arko Jatmiko
Faculty of Pharmacy, Universitas Gadjah Mada
Yuniarti, Nunung
Faculty of Pharmacy, Universitas Gadjah Mada
Pramono, Suwijiyo
Faculty of Pharmacy, Universitas Gadjah Mada
ginger, curcuminoid, analgesic, pain
Penelitian dilakukan untuk mengetahui efek analgetik dari minyak atsiri rimpang temulawak (Curcuma xanthorriza Roxb.), kurkuminoid, dan kombinasi keduanya. Penyarian kurkuminoid dilakukan dengan pelarut etil asetat dan heksan. Minyak atsiri diperoleh dari hasil destilasi air-uap. Uji analgetik dilakukan dengan metode formalin tes (20µL formalin 1%, intraplantar) terhadap mencit jantan galur Swiss (30±5g), umur 2 bulan. Kelompok uji meliputi CMC-Na 0,5 % p.o. (kontrol negatif), indometasin 4 mg/kgBB p.o. dan i.p. (kontrol positif), kurkuminoid 48 mg/kgBB p.o., minyak atsiri 24mg/kgBB p.o, serta kombinasinya p.o. Injeksi formalin dilakukan 1 jam setelah pemejanan sampel. Data licking time diambil pada fase I (menit 0-5) dan fase II (10-30menit) setelah injeksi formalin. Fase I mengindikasikan nyeri yang terjadi pada daerah susunan saraf pusat (SSP) sedangkan fase II pada daerah perifer. Hasil uji terhadap fase I menunjukkan daya analgetik indometasin p.o sebesar (4,96±3,26 %) dan i.p. (4,54±1,65%). Kurkuminoid dan minyak atsiri masing-masing (1,22±0,89%) dan (28,8±8,48%), sedangkan campuran (42,14±5,6%). Kurniawan (2007) melaporkan bahwa besarnya daya analgetik fase I pada morfin 5 mg/kgBB p.o. adalah (69,68±2,17%). Pada fase II minyak atsiri memiliki daya analgetik sebesar (74,63±1,97%), campuran minyak atsiri (24mg/kgBB) dan kurkuminoid (48 mg/kgBB) memiliki daya analgetik sebesar (67,56±0,59%), indometasin i.p. (49,46±1,08%) dan p.o (49,82±0,91%), serta kurkuminoid (44,80±1,46%).
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 10:23:01
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/7745
Majalah Obat Tradisional; Vol 20, No 1 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7746
2015-11-18T03:23:01Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
ANTIOXIDANT ACTIVITY DETERMINATION Garcinia dulcis (Roxb.) Kurz, Blumeamollis (D.Don) Merr., Siegesbeckia orientalis L., AND Salvia riparia H.B.K WHICH COLLECTED FROM TAMAN NASIONAL GUNUNG MERAPI USING DPPH (2,2-diphenyl-1-PIKRIL -HIDRAZIL) AND THIN LAYER
Santosa, Djoko
Faculty of Pharmacy, Universitas Gadjah Mada
Haresmita, Perdana Priya
Faculty of Pharmacy, Universitas Gadjah Mada
Mount Merapi National Park, antioxidant, DPPH, flavonoids, phenols
Penelitian ini bertujuan untuk menentukan aktivitas antioksidan ekstrak wasbenzen dan metanolik empat jenis tumbuhan, yaitu Garcinia dulcis (Roxb.)Kurz, Blumea mollis (D.Don) Merr., Siegesbeckia orientalis L.,dan Salvia riparia H.B.K hasil koleksi dari TNGM berdasarkan metode penangkapan radikal bebas dengan DPPH dan mengetahui profil kromatografi lapis tipisnya. Ekstrak dibuat dengan proses maserasi bertingkat dengan penyari wasbenzen dan metanol, uji aktivitas antioksidan dengan metode DPPH serta analisis kualitatif dengan kromatografi lapis tipis (KLT) untuk tumbuhan dengan aktivitas antioksidan tertinggi. Hasil penelitian menunjukkan bahwa diantara keempat ekstrak metanolik, ekstrak Garcinia dulcis (Roxb.) Kurz mempunyai aktivitas antioksidan tertinggi dengan nilai IC50 38,613µg/mL. Untuk ekstrak wasbenzen, ekstrak Garcinia dulcis (Roxb.) Kurz juga mempunyai aktivitas tertinggi dengan nilai IC50 139,381µg/mL. Analisis kualitatif ekstrak metanolik Garcinia dulcis (Roxb.) Kurz dengan kromatografi lapis tipis (KLT) menunjukkan bahwa ekstrak metanolik Garcinia dulcis (Roxb.) Kurz mengandung senyawa golongan fenol dan flavonoid.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 10:23:01
https://journal.ugm.ac.id/TradMedJ/article/view/7746
Majalah Obat Tradisional; Vol 20, No 1 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7747
2015-11-18T03:23:01Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
EFFECT OF Psidium guajava Linn. Leaves AND Anacardium occidentale Linn. Leaves ON WOUND HEALING TO Mus musculus Linn. SKIN
Hasibuan, Fitri Nirwana
Magister of Biology, Faculty of Mathematics and Natural Science, Diponegoro University
Yuniwarti, E. Yusuf Wachidah
Magister of Biology, Faculty of Mathematics and Natural Science, Diponegoro University
Suedy, S. Widodo Agung
Magister of Biology, Faculty of Mathematics and Natural Science, Diponegoro University
injury, mice, leaves, guava, cashew
Tujuan dari penelitian ini adalah untuk mengkaji khasiat daun jambu biji dan daun jambu mete sebagai obat alternatif untuk pengobatan luka. Penelitian ini menggunakan Rancangan Acak Lengkap (RAL) yang dibagi menjadi 5 perlakuan dengan 4 ulangan, K0 sebagai kontrol menggunakan B®, K1 menggunakan daun jambu biji dengan pelarut air, K2 menggunakan daun jambu biji dengan pelarut alkohol, K3 menggunakan daun jambu mete dengan pelarut air, K4 menggunakan daun jambu mete dengan pelarut alkohol. Metode yang digunakan adalah dengan cara menghaluskan masing-masing perlakuan K1, K2, K3 dan K4 sebanyak 100mg/200ml, kemudian diendapkan selama lima hari di dalam lemari es. Pasta obat didapatkan dengan memisahkan antara cairan dan endapan, kemudian pasta obat dapat dioleskan pada mencit yang telah diberi luka sepanjang 2 cm pada bagian punggung setiap pagi dan sore. variabel yang digunakan adalah lama penyembuhan, jumlah leukosit, neutrofil, limfosit dan rasio N/L. Hasil penelitian menunjukkan bahwa pada lama penyembuhan tidak terdapat beda nyata (P<0,05) antara kontrol dengan perlakuan yaitu pada kontrol selama 14,00±1,63 hari, daun jambu biji dengan pelarut air 12,25±4,50 hari, daun jambu biji dengan pelarut alkohol 10,50±4,35 hari, daun jambu mete dengan pelarut air 12,50±1,00 hari, dan daun jambu mete dengan pelarut alkohol 11,00±3,91 hari. Tingkat stres yang dihasilkan juga menunjukkan perbedaan yang tidak nyata (P<0,05) antara perlakuan dan kontrol yaitu pada kontrol N/L sebesar 1,95±0,11, daun jambu biji dengan pelarut air 1,98±0,20, daun jambu biji dengan pelarut alkohol 2,30±0,09, daun jambu mete dengan pelarut air 1,84±0,30, dan daun jambu mete dengan pelarut alkohol 1,95±0,20. Berdasarkan hal tersebut maka dapat disimpulkan bahwa daun jambu biji dan daun jambu mete baik dengan pelarut air maupun alkohol dapat digunakan sebagai obat penyembuh luka pada kulit.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 10:23:01
https://journal.ugm.ac.id/TradMedJ/article/view/7747
Majalah Obat Tradisional; Vol 20, No 1 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7748
2015-11-18T03:23:01Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
LOZENGES FORMULATION OF MIXED EXTRACT OF Curcuma xanthorrhiza Roxb AND Kaempferia galanga L USING COMBINATION OF FILLER AGENTS MANITOL – MALTODEXTRIN
Prasetyo, Widodo Ndaru
Faculty of Pharmacy, Universitas Gadjah Mada
Mufrod, Mufrod
Faculty of Pharmacy, Universitas Gadjah Mada
C. xanthorrhiza, K. galanga, filler agent, lozenges
Campuran rimpang temulawak dan kencur sering digunakan sebagai penambah nafsu makan.Namun bentuk sediaan yang selama ini digunakan kurang praktis dan tidak stabil dalam penyimpanan. Oleh karena itu perlu dibuat sediaan tablet hisap yang lebih praktis dan acceptable. Tujuan penelitian ini adalah untuk mengetahui pengaruh variasi bahan pengisi manitolmaltodextrin terhadap sifat fisik granul, sifat fisik tablet dan rasa tablet.Ekstrak temulawak dan kencur dibuat dengan metode perkolasi menggunakan etanol 70%, kemudian digranul dengan metode granulasi basah, dibuat menjaditiga formuladengan kombinasi bahan pengisi manitol-maltodekstrin (100:0, 85:15, 70:30). Hasil penelitian menunjukkan kombinasi bahan pengisi manitol-maltodextrin berpengaruh terhadap waktu alir, kompaktibilitas pada sifat fisik granul, dan sifat fisik tablet hisap, kecuali pada uji penampilan. Hasil uji tanggap rasa menunjukkan bahwa responden merasakan formula I asam, sedangkan formula II dan III menunjukkan rasa pahit.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 10:23:01
https://journal.ugm.ac.id/TradMedJ/article/view/7748
Majalah Obat Tradisional; Vol 20, No 1 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7749
2015-11-18T03:23:01Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
MUCOLYTIC IN VITRO ACTIVITY OF ETHANOLIC EXTRACT OF Hibiscus rosa-sinensis L. RED STRAIGHT CROWN
Permatasari, Dinar Hesti
Faculty of Pharmacy, Universitas Gadjah Mada
Murrukmihadi, Mimiek
Faculty of Pharmacy, Universitas Gadjah Mada
mucolytic, syrup, ethanolic extract, red shoe flower
Ekstrak etanolik bunga kembang sepatu warna merah telah terbukti dapat menurunkan viskositas mukus secara in vitro. Tujuan dari penelitian ini adalah untuk mengetahui besarnya pengaruh varian konsentrasi ekstrak dalam menurunkan viskositas mukus dalam sirup secara in vitro dan untuk mengetahui besarnya konsentrasi ekstrak yang sebanding dengan sirup asetilsistein 2,00%. Ekstrak etanolik diperoleh dengan menggunakan metode maserasi kemudian dibuat dalam sediaan sirup dengan konsentrasi ekstrak yang bervariasi (1,00; 1,25; 1,50; 1,75 dan 2,00%). Hasil penelitian menunjukkan bahwa sirup dengan konsentrasi ekstrak 1,00; 1,25; 1,50; 1,75; dan 2,00% tidak menunjukkan adanya aktivitas mukolitik secara in vitro dengan menurunkan viskositas mukus usus sapi dan tidak ada yang sebanding dengan aktivitas mukolitik asetilsistein 2,00%.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 10:23:01
https://journal.ugm.ac.id/TradMedJ/article/view/7749
Majalah Obat Tradisional; Vol 20, No 1 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7750
2015-11-18T03:23:01Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
OPTIMIZATION FORMULA DISPERSIBLE TABLETS OF GUAJAVA LEAF EXTRACT (Psidium guajava L.) WITH COMBINATION DISINTEGRANTS OF CROSCARMELLOSE SODIUM AND SODIUM STARCH GLYCOLATE
Rachmawati, Aditya Novera
Faculty of Pharmacy, Universitas Gadjah Mada
Wahyono, Wahyono
Faculty of Pharmacy, Universitas Gadjah Mada
Sulaiman, T. Nanda Saifullah
Faculty of Pharmacy, Universitas Gadjah Mada
Dispersible tablets, CCS-SSG, Psidium guajava, simplex lattice design
Khasiat dan keamanan daun jambu biji (Psidium guajava L.) sebagai antidiare telah diuji secara praklinik dan klinik. Bentuk sediaan yang ada saat ini kebanyakan susah ditelan, waktu disintegrasinya lama serta memiliki rasa dan bau yang kurang enak, sehingga perlu diformulasikan sediaan yang lebih akseptabel bagi anak-anak. Penelitian ini bertujuan untuk mengoptimasi formula tablet dispersible ekstrak air daun jambu biji menggunakan variasi bahan penghancur croscarmellose sodium (CCS) dan sodium starch glycolate (SSG) dengan metode simplex lattice design (SLD). Tablet dispersible dibuat 5 formula dengan metode granulasi kering yaitu formula I (100% CCS), II (75% CCS : 25% SSG), III (50% CCS : 50% SSG), IV (25% CCS : 75% SSG) dan V (100% SSG). Standarisasi ekstrak yang dilakukan meliputi uji organoleptik, susut pengeringan dan kromatografi lapis tipis (KLT). Tablet dispersible yang diperoleh, diuji sifat fisik keseragaman bobot, kerapuhan, kekerasan, waktu disintegrasi, waktu dispersi, waktu pembasahan serta KLT. Data dianalisis menggunakan software Design Expert® versi 7.1.4.0 untuk mendapatkan formula optimum. Hasil penelitian menunjukkan bahwa kombinasi bahan penghancur CCS-SSG mempengaruhi sifat fisik tablet. Semakin besar jumlah SSG maka semakin besar kekerasan, waktu disintegrasi, waktu dispersi dan waktu pembasahan tablet. Formula optimum yang diperoleh adalah formula dengan kombinasi 71% CCS dan 29% SSG.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 10:23:01
https://journal.ugm.ac.id/TradMedJ/article/view/7750
Majalah Obat Tradisional; Vol 20, No 1 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7751
2015-11-18T03:23:01Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
SCRINING IN SILICO ACTIVE COMPOUND OF Pachyrrhizus erosus AS ANTITIROSINASE ON Aspergillus oryzae (COMPUTATTIONAL STUDY WITH HOMOLOGY MODELING AND MOLECULAR DOCKING)
Lukitaningsih, Endang
Faculty of Pharmacy, Universitas Gadjah Mada
Wisnusaputra, Aditya
Faculty of Pharmacy, Universitas Gadjah Mada
Sudarmanto, B. S. Ari
Faculty of Pharmacy, Universitas Gadjah Mada
anti tyrosinase, homology modeling, Bengkoang (Pachyrrhizus erosus), molecular docking
Bengkoang telah banyak digunakan dalam industri kosmetika sebagai whitening agent. Berdasarkan penelitian Lukitaningsih (2009), bengkoang mengandung 6 senyawa aktif yang mampu berperan sebagai whitening agent dengan menghambat aktivitas enzim tirosinase dari jamur Aspergillus oryzae (TyrAo). Namun interaksi senyawa aktif bengkoang dalam menghambat enzim tirosinase belum dapat diketahui. Interaksi senyawa-senyawa aktif bengkoang dengan enzim TyrAo dapat diketahui dengan studi komputasional (in silico). Pemodelan interaksi senyawa aktif bengkoang dengan enzim TyrAo dilakukan dengan metode homology modeling dan molecular docking. Homology modeling dilakukan untuk memodelkan struktur tiga dimensi (3D) enzim tirosinase Aspergillus oryzae (TyrAo) melalui template berupa protein homolog yang sudah diketahui struktur 3D-nya yaitu enzim TyrAb (PDBID: 2Y9X). Model TyrAo digunakan sebagai target makromolekul dalam metode molecular docking. Metode molecular docking merupakan metode untuk menggambarkan posisi ligan (senyawa-senyawa aktif bengkoang) pada sisi aktif reseptor (model TyrAo). Berdasarkan docking yang dilakukan diketahui bahwa residu-residu yang banyak berpengaruh pada interaksi ligan pada sisi aktif adalah residu Thr275 yang berinteraksi secara ikatan hidrogen dengan ligan dan residu His294 yang berinteraksi secara hidrofobik pada cincin aromatik ligan. Penelitian in silico dan in vitro yang telah dilakukan memiliki korelasi (R2) sebesar -0,8366. Korelasi ini menandakan bahwa aktivitas senyawa-senyawa aktif pada bengkoang dalam menghambat enzim TyrAo memiliki hasil yang serupa pada penelitian yang dilakukan secara in silico dan in vitro.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 10:23:01
https://journal.ugm.ac.id/TradMedJ/article/view/7751
Majalah Obat Tradisional; Vol 20, No 1 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7752
2015-11-18T03:23:01Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
THE EFFECT OF ETHANOL EXTRACT of Ulva Lactuca L ON SGPT-SGOT ACTIVITY IN RAT
Pertiwi, Puteriragil Atma
Faculty of Pharmacy, University of Ahmad Dahlan
Widyaningsih, Wahyu
Faculty of Pharmacy, University of Ahmad Dahlan
Ulva lactuca L., AST, ALT, hepatoprotective
Kerusakan pada hepar dapat ditunjukkan oleh aktivitas enzim seluler yang semakin meningkat. SGOT dan SGPT adalah dua macam enzim yang paling sering dihubungkan dengan kerusakan sel hepar. Salah satu penyebab kerusakan hepar adalah senyawa radikal bebas yaitu CCl4. Ganggang hijau (Ulva lactuca L.) memiliki senyawa melatonin yang bersifat anti oksidan. Penelitian ini bertujuan mengetahui kemampuan ekstrak etanol ganggang hijau (Ulva lactuca L.) sebagai hepatoprotektor dengan menurunkan kadar SGOT dan SGPT akibat senyawa radikal bebas yaitu CCl4. Hewan uji dibagi menjadi 5 kelompok. Kelompok I (kontrol normal) hanya diberi makan dan minum, kelompok II diberi suspensi CMC-Na 1% sebagai kontrol pelarut, kelompok III diberi suspensi ekstrak etanol ganggang hijau dengan dosis 100mg/kgBB/hari, kelompok IV diberi suspensi ekstrak etanol ganggang hijau dengan dosis 200mg/kgBB/hari, dan kelompok V larutan pembanding tablet curcuma sebagai kontrol pembanding dengan dosis 200mg/kgBB/hari. Perlakuan dilakukan selama 21 hari secara per oral, pada hari ke-22 diberi suntikan CCl4 1,0mL/kgBB secara intraperitoneal kecuali kelompok I. Pengambilan darah melalui sinus orbitalis untuk diukur aktivitas SGOT dan SGPT. Hasil yang diperoleh dianalisis statistik menggunakan uji One Way ANOVA dan dilanjutkan dengan uji LSD dengan taraf kepercayaan 95 %. Hasil penelitian dapat disimpulkan bahwa ekstrak etanol ganggang hijau (Ulva lactuca L.) dapat menurunkan aktivitas SGOT dan SGPT pada tikus jantan putih yang diinduksi CCl4.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 10:23:01
https://journal.ugm.ac.id/TradMedJ/article/view/7752
Majalah Obat Tradisional; Vol 20, No 1 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7753
2015-11-18T03:23:01Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
THE EFFECT OF MEDIUM ON ENDOPHYTIC FUNGUS IP-2 GROWTH AND PRODUCTION OF ITS ACTIVE INHIBITOR HEME POLYMERIZATION METABOLITE
Purwantini, Indah
Faculty of Pharmacy, Universitas Gadjah Mada
Wahyono, Wahyono
Faculty of Pharmacy, Universitas Gadjah Mada
Mustofa, Mustofa
Faculty of Medicine, Universitas Gadjah Mada
Susidarti, Ratna Asmah
Faculty of Pharmacy, Universitas Gadjah Mada
endophytic fungi IP-2, fermentation media, heme polymerization inhibitors
Fungi endofit IP-2 adalah fungi endofit yang diisolasi dari tanaman Artemisia annua L. Fungi ini diketahui mempunyai aktivitas sebagai inhibitor polimerisasi hem. Dalam penelitian ini akan diteliti mengenai media yang sesuai untuk pertumbuhan maupun pembentukan metabolit aktifnya serta menentukan waktu yang sesuai untuk pemanenan metabolit aktifnya. Fungi endofit difermentasi dalam 4 jenis media selama 14 hari. Setiap hari dilakukan pengambilan sampel yang akan digunakan untuk mengetahui bobot sel keringnya yang selanjutnya digunakan untuk analisis pertumbuhannya serta digunakan untuk uji aktivitas inhibitor polimerisasi. Uji dilakukan dengan menggunakan metoda yang dikembangkan oleh Bassilico dkk (1998). Data bobot sel kering dan prosentase penghambatan inbihitor polimerisasi dianalisis sehingga diperoleh kurva pertumbuhan dan kurva produksi metabolitnya yang akan digunakan untuk menentukan waktu yang tepat untuk memanen metabolit aktifnya. Hasil penelitian menunjukkan bahwa dalam media PDB fungi endofit IP-2 menunjukkan pertumbuhan yang paling baik dan mampu menghasilkan metabolit aktif paling tinggi dibandingkan 3 media lainnya. Dalam media PDB, pada hari ke-7 terlihat jumlah metabolit aktif yang dihasilkan paling banyak sehingga waktu panen metabolit yang tepat adalah pada hari ke-7.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 10:23:01
https://journal.ugm.ac.id/TradMedJ/article/view/7753
Majalah Obat Tradisional; Vol 20, No 1 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7754
2015-11-18T07:48:41Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
ACTIVITIES OF YAM STARCH (Pachyrhizus erosus (L.) Urban) AS SUNSCREEN IN MOUSE AND THE EFFECT OF ITS CONCENTRATION TO VISCOSITY LEVEL
Zulkarnain, A. Karim
Faculty of Pharmacy, Universitas Gadjah Mada
Ernawati, Novi
Faculty of Pharmacy, Universitas Gadjah Mada
Sukardani, Nurul Ikka
Faculty of Pharmacy, Universitas Gadjah Mada
yam starch, cream, lotion, stability, sunscreen
Amilum bengkuang (Pachyrhizus erosus (L.) Urb.) dengan ukuran mikropartikel dan mempunyai sifat yang opaque dapat dimanfaatkan sebagai tabir surya fisik. Amilum bengkuang dibuat dalam sediaan krim dan losion dan digunakan secara topikal. Penelitian ini bertujuan untuk mengetahui pengaruh kenaikan konsentrasi amilum bengkuang terhadap viskositas sediaan krim dan losion serta aktivitasnya sebagai tabir surya pada mencit yang dilihat dari nilai Sun Protecting Factor (SPF). Amilum bengkuang diperoleh dengan metode pengendapan, selanjutnya dilakukan karakterisasi dan pengukuran diameter partikel. Pembuatan sediaan krim dan losion masing-masing dalam 3 formula dengan konsentrasi amilum bengkuang 15%, 20%, dan 25%, selanjutnya diamati viskositas dan stabilitas fisik dalam kondisi ekstrim panas dan dingin. Pengamatan selama penyimpanan meliputi warna, bau, homogenitas, dan viskositas. Aktivitas tabir surya ditentukan secara in vivo menggunakan mencit betina galur Swiss Webster yang diinduksi dengan 8-MOP selanjutnya dilihat nilai SPF. Data viskositas pada kondisi ekstrim panas dan dingin dianalisis dengan statistik pada taraf kepercayaan 95%. Hasilnya menunjukkan bahwa kenaikan konsentrasi amilum bengkuang menaikkan viskositas, sedangkan warna, bau, dan homogenitas sediaan tidak berubah selama penyimpanan. Sediaan krim dan losion dengan konsentrasi amilum 15%, 20%, dan 25%, memiliki aktivitas tabir surya secara in vivo pada mencit, dengan nilai SPF berturut-turut sebesar 1,22; 1,52; dan 2,38.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 14:48:41
https://journal.ugm.ac.id/TradMedJ/article/view/7754
Majalah Obat Tradisional; Vol 18, No 1 (2013)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7755
2015-11-18T07:48:41Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
THE TEST OF ANTIOXIDANT ACTIVITY FROM BAWANG MEKAH LEAVES (Eleutherine americana Merr.) USING DPPH (2,2-DIPHENYL-1-PICRYLHYDRAZYL) METHOD
Pratiwi, Dina
Department of Pharmacy, Faculty of Medicine, Universitas Tanjungpura Pontianak
Wahdaningsih, Sri
Department of Pharmacy, Faculty of Medicine, Universitas Tanjungpura Pontianak
Isnindar, Isnindar
Department of Pharmacy, Faculty of Medicine, Universitas Tanjungpura Pontianak
antioxidant activity, bawang mekah leaves, DPPH
Penggunaan dan permintaan terhadap tanaman obat tradisional saat ini semakin bertambah sehingga penelitian ke arah obat-obatan tradisional juga semakin meningkat. Salah satu tanaman yang berkhasiat sebagai obat yaitu bawang mekah (Eleutherine americana Merr.). Tanaman ini biasanya digunakan oleh masyarakat sebagai obat tradisional untuk mengobati berbagai penyakit. Penelitian ini bertujuan untuk mengetahui aktivitas antioksidan dari daun bawang mekah. Pengujian aktivitas antioksidan dilakukan dengan menggunakan metode DPPH (2,2-difenil-1-pikrilhidrazil) yang diawali dengan ekstraksi secara maserasi. Simplisia daun bawang mekah (150 g) direndam dengan etanol 70% selama 3x24 jam pada suhu kamar. Ekstrak etanol cair yang diperoleh diuapkan dengan rotary evaporator dan dipekatkan menggunakan waterbath sehingga diperoleh ekstrak kental. Kemudian terhadap ekstrak dilakukan skrining fitokimia serta uji pendahuluan dengan metode DPPH secara kromatografi lapis tipis (KLT) dengan fase gerak BAA (4:1:5). Aktivitas antioksidan diukur menggunakan spektrofotometer UV-Vis dan dibandingkan dengan vitamin C. Hasil skrining fitokimia menunjukkan ekstrak mengandung senyawa flavonoid, saponin, fenol dan tanin. Pada uji pendahuluan menggunakan KLT, diperoleh 3 spot yang divisualisasi dengan sinar UV 366 nm dan disemprot dengan DPPH 0,2%. Ketiga spot menunjukkan perubahan warna kuning dengan latar belakang ungu yang menandakan ekstrak positif memiliki aktivitas antioksidan. Hasil pengukuran secara spektrofotometri menunjukkan bahwa ekstrak mempunyai IC50 pada 31,97437 µg/ml, sedangkan vitamin C memiliki nilai IC50 yang lebih rendah (3,90186 µg/ml).
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 14:48:41
https://journal.ugm.ac.id/TradMedJ/article/view/7755
Majalah Obat Tradisional; Vol 18, No 1 (2013)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7756
2015-11-18T07:48:41Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
ANALYSIS OF CAROTENOID CONTENT OF CRUDE EXTRACT OF TONGKAT LANGIT BANANA FRUIT (Musa troglodytarum) USING NIR SPECTROSCOPY (NEAR INFRARED)
Samson, Efraim
Department of Biology, Universitas Kristen Satya Wacana
Semangun, Haryono
Department of Biology, Universitas Kristen Satya Wacana
Rondonuwu, Ferdy S.
Department of Biology, Universitas Kristen Satya Wacana
Department of Physics, Universitas Kristen Satya Wacana
Tongkat langit banana, Musa troglodytarum, Carotenoids, NIR Spectroscopy
Kandungan karotenoid yang terdapat dalam buah-buahan dan sayuran, berperan penting dalam mencegah penyakit manusia, termasuk penyakit kardiovaskuler, kanker dan penyakit kronis lainnya. Namun, banyak metode yang digunakan dalam penentuan komposisi kimia khususnya untuk bahan makanan membutuhkan waktu yang lama serta biaya cukup mahal. Saat ini sejumlah teknik instrumentasi telah dikembangkan untuk menentukan kandungan kimia dengan cepat. Salah satu contoh adalah teknik pengukuran dengan menggunakan spektroskopi NIR. Dalam penelitian ini, spektroskopi NIR digunakan untuk analisis pendugaan karotenoid ekstrak kasar buah pisang tongkat langit (Musa troglodytarum) yang panjang dan pendek. Hasil yang diperoleh menunjukkan pisang tongkat langit mengandung β-karoten, sebab pada area bilangan gelombang 5000 hingga 5500 cm-1 terlihat adanya kemiripan antara pola spektrum β-karoten marker dengan pola spektrum pigmen karotenoid pisang tongkat langit.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 14:48:41
https://journal.ugm.ac.id/TradMedJ/article/view/7756
Majalah Obat Tradisional; Vol 18, No 1 (2013)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7757
2015-11-18T07:48:41Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
SELECTIVITY OF PURIFIED EXTRACT FROM THE LEAVES OF Tithonia diversifolia (Hemsley) A.Gray AGAINST HELA CELLS
Wahyuningsih, M. Sri Hartati
Faculty of Medicine, Universitas Gadjah Mada
Syarif, Rul Afiyah
Faculty of Medicine, Universitas Gadjah Mada
Suharmi, Sri
Faculty of Medicine, Universitas Gadjah Mada
Murini, Tri
Faculty of Medicine, Universitas Gadjah Mada
Saputra, Firandi
Faculty of Medicine, Universitas Gadjah Mada
W, Adiguno Suryo
Faculty of Medicine, Universitas Gadjah Mada
Extraction, purification, T. diversifolia, HeLa cell, IC50
Tanaman kembang bulan [Tithonia diversifolia (Hemsley) A.Gray] merupakan salah satu tanaman yang digunakan dalam pengobatan tradisional untuk berbagai penyakit. Penelitian sebelumnya menyebutkan bahwa kembang bulan memiliki efek antiproliferasi pada sel kanker kolon (Col2). Ekstraksi dan partisi termonitor dengan uji aktivitas merupakan metode untuk mengambil senyawa aktif yang terkandung dalam ekstrak. Penelitian tentang selektivitas ekstrak terpurifikasi dari ekstrak aktif daun Kembang bulan terhadap sel HeLa belum pernah diteliti sebelumnya. Tujuan penelitian ini untuk mengetahui selektivitas ekstrak terpurifikasi daun Kembang Bulan pada sel HeLa dibandingkan dengan sel Vero dan menentukan nilai IC50 serta indeks selektivitasnya. Daun kembang bulan diekstraksi menggunakan pelarut Kloroform dan Metanol. Kedua ekstrak diuji efek sitotoksiknya pada sel Hela dengan beberapa konsentrasi (0,12 s/d 250μg/mL) menggunakan metode MTT (Mosmann, 1983). Pembacaan densitas optik dengan ELISA plate reader. Persentase kematian sel dihitung dan dianalisis dengan menggunakan regresi probit pada program SPSS 15 for Windows. Setelah diketahui nilai IC50 kedua ekstrak tersebut, dipilih salah satu yang memiliki IC50 lebih kecil kemudian dipurifikasi dengan Petroleum Eter (PE) dan diuji kembali pada sel HeLa, sari yang aktif diuji juga selektivitasnya dengan sel Vero, kemudian dihitung nilai IC50 nya. Nilai IC50 ekstrak metanol sebesar 1006,99μg/mL, ekstrak Kloroform sebesar 16,61μg/mL. Nilai IC50 sari larut PE sebesar 325,331μg/mL dan IC50 sari tidak larut PE sebesar 3,078μg/mL dan nilai IC50 pada sel vero adalah 80,30μg/mL. Nilai indeks selektivitas ekstrak terpurifikasi (sari tidak larut PE) adalah 26.09.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 14:48:41
https://journal.ugm.ac.id/TradMedJ/article/view/7757
Majalah Obat Tradisional; Vol 18, No 1 (2013)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7758
2015-11-18T07:48:41Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
CYTOTOXICITY STUDY OF MEZZETIAPARVIFLORABECC. WOODBARK
Mufidah, Mufidah
Faculty of Pharmacy, Hasanuddin University
Wahyudin, Elly
Faculty of Pharmacy, Hasanuddin University
Lawrence, Gatot S.
Faculty of Medicine, Hasanuddin University
Wahyuningsih, M. Sri Hartati
Faculty of Medicine, Universitas Gadjah Mada
Manggau, Mirianti A.
Faculty of Pharmacy, Hasanuddin University
Mezzetiaparviflora Becc., MTT, Cytotoxicity, Vero cell line, HeLa cell line
Aktivitas sitotoksik ekstrak larut aseton batang kayu MezzetiaparvifloraBecc. dievaluasi pada sel HeLa dan dibandingkan dengan sel normal vero untuk memastikan penggunaan sebagai tanaman obat tradisional untuk pengobatan tumor. Percobaan dengan sel normal vero menggunakan uji MTT menunjukkan persentase viabilitas sel sebanyak 96,8% pada konsentrasi 1000 ug dan tidak meningkat dengan meningkatnya konsentrasi ekstrak. Sedangkan pada percobaan dengan sel HeLa menunjukkan aktivitas sitotoksik yang rendah dengan persentase viabilitas sebesar 87,4% pada konsentrasi 1000μg/ml. Oleh karena itu ekstrak ini dikategorikan sebagai tidak beracun dan diperlukan studi selanjutnya untuk mengeksplorasi mekanisme yang bertanggung jawabterhadap efek antikanker dari tanaman tersebut.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 14:48:41
https://journal.ugm.ac.id/TradMedJ/article/view/7758
Majalah Obat Tradisional; Vol 18, No 1 (2013)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7759
2015-11-18T07:48:41Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
ISOLATION AND IDENTIFICATION OF ANTIOXIDANT COMPOUNDS IN FERN STEMS (Alsophila glauca J.Sm) USING DPPH METHOD (2,2-DIPHENYL-1-PICRYLHYDRAZYL)
Wahdaningsih, Sri
Department of Pharmacy, Faculty of Medicine and Public Health Sciences, Universitas Tanjungpura Pontianak
Wahyuono, Subagus
Faculty of Pharmacy, Universitas Gadjah Mada
Setyowati, Erna Prawita
Faculty of Pharmacy, Universitas Gadjah Mada
Stem fern, antioxidant, DPPH (2,2-diphenyl-1-picrylhydrazyl)
Stress oksidatif yang diinduksi oleh radikal bebas diketahui dapat mempengaruhi terjadinya berbagai penyakit degeneratif. Sebagai upaya dalam pencarian senyawa antioksidan alami maka telah dilakukan penelitian isolasi dan identifikasi senyawa antioksidan dari batang pakis (Alsophila glauca J.Sm) dengan metode DPPH. Penyarian batang pakis dilakukan secara maserasi dengan wasbenzen dan metanol. Ekstrak didapat dengan menguapkan pelarut wasbenzen dengan rotavapor. Setelah semua wasbenzen menguap bahan dimaserasi lagi dengan metanol. Dengan cara yang sama seperti pada wasbenzen hingga didapat ekstrak metanol. Ekstrak ini diuji aktivitas antioksidannya dengan metode DPPH secara KLT. Ekstrak yang aktif kemudian dipartisi dengan metanol 80%. Ekstrak yang larut dan ekstrak yang tidak larut dalam metanol 80% diuji aktivitas antioksidannya dengan metode DPPH (KLT). Ekstrak yang aktif difraksinasi dengan kromatografi cair vakum dengan menggunakan fase gerak dengan gradient kepolaran yang berbeda (wasbenzen : kloroform) dengan berbagai konsentrasi. Fraksi yang aktif diisolasi dengan metode KLT preparatif dan diperoleh senyawa yang kemurniannya diuji secara KLT. Isolat yang diperoleh diuji aktivitas antioksidannya dengan metode DPPH menggunakan alat spektrofotometri. Diketahui mempunyai aktivitas antioksidan penangkap radikal dengan IC50 178,4µg/mL.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 14:48:41
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/7759
Majalah Obat Tradisional; Vol 18, No 1 (2013)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7924
2015-11-18T07:48:41Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
ANTIFUNGAL ACTIVITIES OF BOILED CASHEW BARK (Anacardium occidentale L.) ON C. albicans IN ACRYLIC RESIN
Lidyawita, Ria
Faculty of Pharmacy, Universitas Gadjah Mada
Sudarsono, Sudarsono
Faculty of Pharmacy, Universitas Gadjah Mada
Harsini, Harsini
Faculty of Medicine, Universitas Gadjah Mada
Anacardium occidentale L., Antifungal, C. albicans, Denture stomatitis
Kurangnya perawatan gigi palsu berakibat pada tingkat kepadatan koloni Candida albicans yang dapat menyebabkan denture stomatitis. Mikroba tersebut sering dijumpai pada pemakai gigi tiruan dan dijumpai sebanyak 86% dari penderita denture stomatitis. Pada kulit batang jambu mete terdapat tanin, asam galat dan asam anakardat, yaitu komponen fenolik dengan aktivitas antijamur terhadap C. albicans. Penelitian ini meliputi pengumpulan bahan, perebusan, freeze drying, identifikasi fitokimia asam anakardat dan asam galat, penetapan kandungan fenolik total dengan metode Folin Ciocalteu, dan uji daya antifungi terhadap C. albicans pada plat resin akrilik. Plat resin akrilik direndam dalam suspensi C. albicans selama 24 jam, dibilas dengan Phosphate Buffer saline (PBS), direndam disertai agitasi selama 3 menit dalam larutan hasil freeze drying rebusan kulit batang jambu mete konsentrasi 12,5%b/v, 25%b/v, dan 50%b/v dengan stok 20mg/mL, divibrasi dalam NaCl fisiologis yang kemudian diinkubasi pada Agar Sabouraud selama 48 jam, diamati dan dihitung persentase penghambatan pertumbuhan C. albicans. Analisis statistik dengan Anava, dilanjutkan uji Kruskal-Wallis untuk mengetahui perbedaan antar kelompok perlakuan. Hasil penelitian menunjukkan bahwa rebusan kulit batang jambu mete konsentrasi 12,5%b/v, 25%b/v, dan 50%b/v memiliki daya hambat terhadap pertumbuhan C. albicans yang berbeda bermakna, yaitu berturut – turut 69,69%, 82,66%, 94,14% dan kandungan fenolik total yang berbeda bermakna, yaitu berturut – turut 35,18%b/b EAG, 31,64%b/b EAG, 29,47%b/b EAG.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 14:48:41
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/7924
Majalah Obat Tradisional; Vol 18, No 1 (2013)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7944
2015-11-18T07:48:41Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
ANTIMICROBIAL POTENCY OF ANT-PLANT EXTRACT (Myrmecodia tuberosa JACK.) AGAINST Candida albicans, Escherichia coli, AND Staphylococcus aureus
Efendi, Yuli Nurullaili
Faculty of Pharmacy, Universitas Gadjah Mada
Hertiani, Triana
Faculty of Pharmacy, Universitas Gadjah Mada
M. tuberosa ethanol extract, C. albicans, E. coli, S. aureus, antimicroba
Pentingnya pencarian sumber baru senyawa aktif untuk mengatasi penyakit infeksi mendorong dilakukannya penelitian untuk mengetahui aktivitas antimikroba ekstrak etanol tumbuhan Sarang semut. Tumbuhan ini telah secara luas dipergunakan dalam pengobatan tradisional masyarakat Papua Barat. Penelitian ini bertujuan untuk mengetahui potensi antimikroba ekstrak etanol tumbuhan Sarang semut terhadap C. albicans, bakteri E. coli, dan S. aureus dan mengetahui golongan senyawa aktif yang bertanggung-jawab terhadap aktivitas tersebut. Ekstrak etanol diperoleh dengan merendam serbuk kering M. tuberosa dalam etanol 70% teknik yang dilanjutkan dengan penguapan penyari. Skrining aktivitas antimikroba terhadap C. albicans, bakteri E. coli, dan S. aureus dilakukan terhadap ekstrak kental yang didapatkan dengan metode difusi padat. Pengujian dengan menggunakan metode mikrodilusi dilakukan untuk mengetahui harga KHM ekstrak, dilanjutkan pengukuran nilai KBM pada media padat yang sesuai. Untuk mengetahui golongan senyawa yang bertanggung jawab terhadap aktivitas antimikroba digunakan metode uji bioautografi kontak. Hasil penelitian menunjukkan harga KHM ekstrak terhadap C. albicans, E. coli, dan S. aureus masing-masing secara berurutan adalah 0,8%b/v; 0,8%b/v; dan 1,6% b/v. Nilai KBM terhadap C. albicans >6,4% b/v, E. coli 6,4% b/v, dan S. aureus 1,6% b/v. Hasil uji bioautografi pada KLT (silika gel 60 F254, toluen: aseton: metanol: asam formiat (26:8:5:1) v/v) menunjukkan bercak aktif terhadap E. coli dan S. aureus memiliki harga hRf 0 sedangkan bercak aktif terhadap C. albicans dan S. aureus pada hRf 53. Pada kedua bercak terdeteksi senyawa fenolik. Dapat disimpulkan bahwa ekstrak etanol M. tuberosa mengandung senyawa-senyawa yang berpotensi untuk dikembangkan sebagai antimikroba khususnya terhadap S. aureus.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 14:48:41
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/7944
Majalah Obat Tradisional; Vol 18, No 1 (2013)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7945
2015-11-18T07:48:41Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
INFUSE OF Costus speciosus (KOEN.) JE SMITH LEAF AS AN INHIBITOR OF SPERMATOZOA QUANTITY AND QUALITY OF MALE MICE BALB / C
Sari, Ika Puspita
Faculty of Pharmacy, Universitas Gadjah Mada
Rahayu, Siti
Faculty of Pharmacy, Universitas Gadjah Mada
Rizal, Dicky M.
Faculty of Medicine, Universitas Gadjah Mada
infusion infusa, pacing leaves, number spermatozoa, qualities spermatozoa
Costus speciosus(Koen.)J.E. Smith (Pacing) merupakan tanaman yang dimanfaatkan oleh masyarakat sebagai obat tradisional untuk kontrasepsi. Penelitian ini bertujuan untuk mengetahui pengaruh infusa daun Pacing terhadap spermatogenesis mencit jantan strain Balb/Cserta reversibilitas efeknya sebagai dasar dari pembuktian secara ilmiah kemampuan daun Pacing sebagai calon obat kontrasepsi khususnya pada individu jantan. Mencit dikelompokkan menjadi 6 kelompok masing-masing terdiri atas 6 ekor mencit. Infusa daun Pacing diberikan dengan variasi dosis yakni 275, 550 dan 1100 mg/kgBB, sementara itu kelompok kontrol terdiri atas kelompok pemberian akuades, kelompok pemberian minyak jagung dan kelompok Andriol dosis 5,2 mg/kgBB. Semua sediaan uji diberikan secara oral setiap hari selama 14 hari. Pada hari ke 15 hewan uji dalam semua kelompok dikorbankan masing-masing 3 ekor mencit kemudian dilakukan pembedahan, 3 ekor mencit sisanya dipelihara hingga hari ke 28 tanpa pemberian sediaan uji untuk mengetahui reversibilitas efek spermatogenesisnya. Pengamatan efek spermatogenesis dilakukan terhadap jumlah dan kualitas spermatozoa (motilitas, viabilitas dan morfologi). Data jumlah dan kualitas spermatozoa dianalisis secara statistik menggunakan uji non-parametrik Kruskal-Wallis, dilanjutkan dengan uji Mann-Whitney pada taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa pemberian infusa daun Pacing pada dosis 275, 550 dan 1100 mg/kg BB mampu menurunkan jumlah spermatozoa sebesar 16-38 % (P<0,05), namun tidak mengubah viabilitas dan morfologi spermatozoa. Selain itu, pada dosis 275 dan 550 mg/kg BB, infusa daun Pacing mampu menurunkan motilitas spermatozoa sebesar 36-39% (P<0,05). Kemampuan infusa daun Pacing dalam menurunkan jumlah dan motilitas spermatozoa bersifat reversible setelah 14 hari kemudian.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 14:48:41
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/7945
Majalah Obat Tradisional; Vol 18, No 1 (2013)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7946
2015-11-18T07:48:41Z
TradMedJ:ART
"151118 2015 eng "
2406-9086
1410-5918
dc
SHOOT CULTURE OF Scoparia dulcis, Lindernia anagalis, Lindernia ciliata AND THE EFFORT OF BIOREMEDIATION FOR HEAVY-METAL PB, CR, CD
Santosa, Djoko
Faculty of Pharmacy, Universitas Gadjah Mada
Sabila, Nurma
Faculty of Pharmacy, Universitas Gadjah Mada
Dewi, Irma Puspita
Faculty of Pharmacy, Universitas Gadjah Mada
Aini, Luthfia Nurul
Faculty of Pharmacy, Universitas Gadjah Mada
abioremediation, shoot culture, Scoparia dulcis, Lindernia anagalis, Lindernia ciliata
Telah dilakukan penelitian tentang kultur tunas Scoparia dulcis, Lindernia anagalis, Lindernia ciliata untuk mengetahui kemampuan penyerapan logam berat Pb, Cr dan Cd sebagai proses bioremediasi terhadap polusi logam berat.Tunas-tunas tersebut ditumbuhkan pada media Murashige-Skoog (MS) padat yang ditambah kinetin 2 ppm. Subkultur dilakukan terhadap semua jenis tunas pada umur 21 hari kemudian dikultivasi pada media MS padat ditambah logam berat 0, 2 dan 5 ppm selama 30 hari. Semua tunas mampu menyerap Cr tetapi tidak mampu menyerap Cd, tunas Lindernia anagalis dan Lindernia ciliata mampu menyerap Pb.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 14:48:41
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/7946
Majalah Obat Tradisional; Vol 18, No 1 (2013)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7981
2015-11-24T05:21:55Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
MACROSCOPY, MICROSCOPY, PHYTOCHEMICAL IDENTITY OF C. Mangga, C. Zedoaria, and K. Rotunda Rhizome
Mulyani, Sri
Faculty of Pharmacy, Universitas Gadjah Mada
Nugraheni, Novia Dwi
Faculty of Pharmacy, Universitas Gadjah Mada
Sari, Hendri Marinda
Faculty of Pharmacy, Universitas Gadjah Mada
Siswondo, A. Zulvita Anani
Faculty of Pharmacy, Universitas Gadjah Mada
macroscopic, microscopic, chemical content, temu putih
Di Indonesia tumbuhan dari suku Zingiberaceae digunakan secara luas untuk pengobatan tradisional, seperti Curcuma mangga, C. zedoaria, dan Kaempferia rotunda. Ketiganya di Indonesia dikenal dengan nama daerah temu putih. Penelitian ini bertujuan untuk mengetahui ciri makroskopi, mikroskopi, dan kandungan kimianya, agar dapat digunakan sebagai alat bantu identifikasi. Pemeriksaan makroskopi dilakukan dengan cara mengamati ciri-ciri dari rimpang baik dalam keadaan segar dan kering, sedang pemeriksaan mikroskopi dilakukan dengan melakukan pengamatan ciri-ciri mikroskopi dari rimpang segar dan serbuk. Ciri kandungan kimia dilakukan dengan pemeriksaan komponen penyusun minyak atsiri dengan metoda GC-MS.Hasil penelitian menunjukkan bahwa secara makroskopi ketiga bahan uji memiliki perbedaan rasa dan bau, pemeriksaan mikroskopi rimpang segar ketiganya memiliki perbedaan ketebalan jaringan gabus, warna sel minyak, dan hasil mikroskopi serbuk menunjukkan perbedaan ukuran amilum. Sebagai senyawa identitas dari minyak C. mangga, C. zedoaria, dan K. rotunda berturut-turut adalah mirsena, germakrona dan benzilbenzoat.
Faculty of Pharmacy, Universitas Gadjah Mada
2013-05-01 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/7981
Majalah Obat Tradisional; Vol 18, No 2 (2013)
ind
Copyright (c) 2013 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7982
2015-11-24T05:21:55Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
EFFECT OF TRAGACANTH AS BINDING AGENTS TO GINGER (Zingiber officinale Roxb.) LOZENGES
Taurina, Wintari
Department of Pharmacy, Faculty of Medicine, Universitas Tanjungpura, Pontianak
Syukri, Yandi
Department of Pharmacy, Faculty of Mathemathics and Natural Sciences, Universitas Islam Indonesia
Triastuti, Asih
Department of Pharmacy, Faculty of Mathemathics and Natural Sciences, Universitas Islam Indonesia
Ginger lozenges, tragacanth, wet granulation method
Jahe (Zingiber officinale Roxb.) merupakan salah satu tanaman obat yang berkhasiat sebagai obat batuk, pelega perut, obat rematik serta penawar racun, dan telah lama digunakan dalam bentuk jamu. Penelitian ini bertujuan untuk membuat sediaan obat tradisional yang lebih praktis, ekonomis, stabil, dan bernilai estetis dalam bentuk tablet hisap ekstrak jahe. Pertama dilakukan ekstraksi dari serbuk jahe, kemudian dibuat ekstrak kental dan diformulasi menjadi tablet hisap menggunakan metode granulasi basah dengan variasi konsentrasi bahan pengikat tragakan berturut-turut adalah 5%, 7,5%, dan 10%. Granul yang dihasilkan diuji sifat fisiknya berikut dievaluasi sifat fisik tablet dan tanggapan rasa serta waktu melarut di dalam mulut. Data yang diperoleh dianalisis menggunakan korelasi Pearson dengan taraf kepercayaan 99%. Semua formula dengan variasi konsentrasi bahan pengikat tragakan dapat menghasilkan sifat fisik tablet hisap yang baik dalam memenuhi persyaratan. Dari hasil penelitian terlihat bahwa semakin besar konsentrasi tragakan yang digunakan menyebabkan % penyimpangan bobot tablet dan kerapuhan semakin kecil, kekerasan akan semakin besar serta memperlama waktu melarut tablet. Diantara formula, tablet dengan konsentrasi tagakan 10% menghasilkan sifat fisik yang paling optimal dengan penyimpangan bobot 0,98%, kekerasan 10,18 kg, dan kerapuhan 0,11% serta waktu melarut 11,50 menit. Pada uji tanggapan rasa ketiga formula sebagian besar diterima responden dengan syarat meningkatkan kemanisan dan memperbaiki bentuk dan warnanya.
Faculty of Pharmacy, Universitas Gadjah Mada
2013-05-01 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/7982
Majalah Obat Tradisional; Vol 18, No 2 (2013)
ind
Copyright (c) 2013 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7983
2015-11-24T05:21:55Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
INTERACTION BETWEEN ACTIVE COMPOUNDS FROM Aegle marmelos CORREA AS ANTI INFLAMMATION AGENT WITH COX-1 AND COX-2 RECEPTOR
Agistia, Dani Dwi
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
Purnomo, Hari
Department Medicinal Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada
Tegar, Maulana
Department Medicinal Chemistry, Faculty of Pharmacy, Universitas Gadjah Mada
Nugroho, Agung Endro
Department of Pharmacology and Clinical Pharmacy, Faculty of Pharmacy, Universitas Gadjah Mada
Marmin, Antiinflamasi, Ligand, COX-1, Docking
Senyawa-senyawa yang terdapat dalam Aegle marmelos Correa memiliki aktivitas sebagai antiinflamasi. Tujuan penelitian ini adalah untuk mengetahui enam senyawa aktif Aegle Marmelos Correa, yaitu (E, R)-Marmin, skimmianine, (S)-aegeline, aurapten, zeorin, dan dustanin sebagai anti inflamasi pada target reseptor COX-1 dan COX-2. Metode: Docking molekuler dilakukan dengan PLANTS. Preparasi ligand menggunakan MarvinSketch, dengan menggambar senyawa MH2011 kemudian mengoptimasi. Preparasi protein dilakukan dengan program YASARA. Preparasi dilakukan dengan menghilangkan protocol docking (termasuk air jika esensial) yang tidak diperlukan. Setelah semua preparasi selesai, dilakukan docking PLANTS. Penurunan skor menunjukkan kestabilan ikatan dengan protein. Hasil: Marmin, skimmianine, aegeline, aurapten, zeorin, and dustanin berpotensial untuk dikembangkan sebagai agen anti inflamasi berdasarkan interaksinya dengan residu asam amino Arg120, Tyr 355, dan Ile523 pada COX-1 dan residu asam amino Ser353, Arg513, serta Ser530 pada COX-2. Kesimpulan: Berdasarkan hasil docking molecular, senyawa aktif Aegle marmelos Correa beraktivitas sebagai antiinflamasi.
Faculty of Pharmacy, Universitas Gadjah Mada
2013-05-01 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/7983
Majalah Obat Tradisional; Vol 18, No 2 (2013)
ind
Copyright (c) 2013 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7998
2015-12-14T09:21:42Z
TradMedJ:ART
"151214 2015 eng "
2406-9086
1410-5918
dc
EFFECT OF STORAGE LENGTH ON PHYSICAL AND CHEMICAL STABILITY OF HAIR GROWTH LOTION CONTAINING CANDLENUT EXTRACT (Aleurites moluccana L. Willd.)
Prasojo, Anas Putro Senu
Faculty of Pharmacy, Universitas Gadjah Mada
Mulyani, Sri
Faculty of Pharmacy, Universitas Gadjah Mada
Mufrod, Mufrod
Faculty of Pharmacy, Universitas Gadjah Mada
Lotion, candlenut (Aleurites moluccana L. Willd.), stability, hair growth
Etanolic extract 70% of candlenut which has 5% concentration have been reported to have hair restoring activity. This study aims to investigate the effect of length storage on physical stability and flavonoid content of hair restoring lotion candlenut extract so that produce an acceptable form. Lotion were made in variation of xanthan gum concentration 0.5%; 1%; 1.5%. Flavonoid content were measured by densitometry. Observation result of physical stability such as latched power, viscosity test, and flavonoid content were statistically analyzed. Result of formula orientation showed that lotion which has 1% concentration of xanthan gum was the best which has latched power less than 1 second, viscosity increased weekly, and segregation was not occur. Type of emulsion result was o/w. Densitometry result showed that there were decrease of relative flavonoid ontent before and after storage, but it was not significant (α = 0.58).
Faculty of Pharmacy, Universitas Gadjah Mada
2015-12-14 16:21:42
https://journal.ugm.ac.id/TradMedJ/article/view/7998
Majalah Obat Tradisional; Vol 17, No 1 (2012)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/7999
2015-12-14T09:21:42Z
TradMedJ:ART
"151214 2015 eng "
2406-9086
1410-5918
dc
APHRODISIAC EFFECTS OF RED GINGER (Zingiber officinale Rosc. red clone) ESSENTIAL OIL AND ESSENTIAL OIL FREE ETANOLIC EXTRACT IN MALE RATS
Anandita, Dipta Wana
Fakultas Farmasi, Universitas Gadjah Mada
Nurlaila, Nurlaila
Fakultas Farmasi, Universitas Gadjah Mada
Pramono, Suwijiyo
Fakultas Farmasi, Universitas Gadjah Mada
Aphrodisiac, red ginger, Zingiber officinale, coitus
Nowadays, many young men experience sexual disfunction due to many factors. Red ginger is traditionally used to solve problems related to sexual disfunction. Oleoresin compound of red ginger has been reported to function as aphrodisiac. This research aims to compare aphrodisiac effect of essential oil and essential oil free extract in male rats. Red ginger essential oil was obtained by destilation while the red ginger free of essential oil was extracted using ethanol 70%. Rats were divided into five groups, administered by : etanolic extract suspension (140 mg/kg BM), essential oil emultion (15 μl/kg BM), pasak bumi extract suspension as positive control (500mg/kg BM), red ginger powder suspension as positive control (2,5 g/kg BM), and CMC Na 1% suspension as negative control. All rats were treated orally for 32 days. Parameters measured were introduction, climbing, and coitus frequency. The result showed that essential oil increased libido but its activity was lower than pasak bumi. Etanolic extract of red ginger and red ginger powder did not increase libido and sexual activity as compared with negative control.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-12-14 16:21:42
https://journal.ugm.ac.id/TradMedJ/article/view/7999
Majalah Obat Tradisional; Vol 17, No 1 (2012)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8000
2015-12-14T09:21:42Z
TradMedJ:ART
"151214 2015 eng "
2406-9086
1410-5918
dc
HARD CANDY LOZENGES FORMULATION OF LEGUNDI (Vitex trifolia L.) LEAF EXTRACT
Alkarim, Mahfud
Faculty of Pharmacy, Universitas Gadjah Mada
Murti, Yosi Bayu
Faculty of Pharmacy, Universitas Gadjah Mada
Sulaiman, T. Nanda Saifullah
Faculty of Pharmacy, Universitas Gadjah Mada
Legundi, Vitex trifolia L., hard candy lozenges, sukrosa, glukosa
The purpose of this study is to determine the effect of sugar level variations (sucrose and glucose) to the physical properties and flavor, as well as to determine the level of consumer preferences on hard candy lozenges of Legundi leaf extract. Hard candy lozenges are produced using the fusion method to create three formulas contain combination of sucrose and glucose, which is FI (70%: 30%), FII (50%: 50%), and FIII (30%: 70%). Hard candy lozenges were tested the physical properties include: uniformity of weight, hardness, moisture content test, a soluble and TLC-densitometry and responsive taste test using a questionnaire with scoring assessment by respondents. The test results were analyzed using SPSS 11.0 for windows with a significance level (α) = 0.05. The results showed that the variation of sucrose and glucose levels affect the physical properties of hard candy lozenges of Legundi leaf extract that increases the hardness level, and increase the time to dissolve (p <0.05) but did not affect the water content of the tablet (p> 0.05). The more sucrose is used it increase the hardness of the tablets and dissolve into the longer time. Based on the perceptive sense test results subjectively of hard candy lozenges of Legundi leaf extract, Formula III is the most favored by respondents with sucrose and glucose ratio of 30%: 70%.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-12-14 16:21:42
https://journal.ugm.ac.id/TradMedJ/article/view/8000
Majalah Obat Tradisional; Vol 17, No 1 (2012)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8003
2015-12-14T09:25:14Z
TradMedJ:ART
"151214 2015 eng "
2406-9086
1410-5918
dc
THE EFFECT OF AVOCADO (Persea americana Mill.) SEED STARCH ONCENTRATION ENHANCEMENT AS A BINDER TO THE PHYSICAL CHARACTERISTICS OF THE GRANULE AND Imperata cylincrical (Linn.) ROOT EXTRACT TABLETS
Kartika, Dyah Hana
Sekolah Tinggi Ilmu Farmasi “YAYASAN PHARMASI” Semarang
Mutmainah, Mutmainah
Sekolah Tinggi Ilmu Farmasi “YAYASAN PHARMASI” Semarang
Mufrod, Mufrod
Faculty of Pharmacy, Universitas Gadjah Mada
Avocado seed starch, binders, tablets, roots of Imperata cylindra (Linn.), Physical characteristics of the granules and tablets
This research aims to determine the effect of avocado seed starch as a binder to the physical characteristics of the granule that is flow rate, angle of repose and moisture content as well as to the physical characteristics of tablets that is uniformity of weight, hardness, friability and disintegration time. Root of Imperata cylindrical (Linn.) extract was obtained by remaceration process usingethanol 70 % as a solvent. Tablets were made by wet granulation method with increase the avocado seed starch concentration as binder tablet (FI: 10 %, FII: 20 %, and FIII: 30 %) then the physical characteristics of granule and tablets were tested. Data were analyzed theoretically by comparing toliterature and statisticaly using one way ANOVA with a level of confidence up to 95 %, followed by Scheffe test. The results showed that the granule’s flow rate andangle of repose decrease byincreasing of avocado seed starch concentrations as a binder, while will increase the moist content of granules. Increasing of avocado seed starch concentration as a binder will increase the hardness and disintegration time of tablets as well, while friability of tablets are decrease and weight of tablets are more uniform.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-12-14 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8003
Majalah Obat Tradisional; Vol 17, No 2 (2012)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8004
2015-12-14T09:25:14Z
TradMedJ:ART
"151214 2015 eng "
2406-9086
1410-5918
dc
EFFECT OF KEPEL LEAVES’ EXTRACT (Stelechocarpus burahol (BL) Hook f. & Th.) CONCENTRATIONON THE ANTIOXIDANT ACTIVITY AND ITS PHYSICAL PROPERTIES WITHIN CREAM FORMULATION
Suwandi, Anita Oktaviani
Faculty of Pharmacy, Universitas Gadjah Mada
Pramono, Suwidjiyo
Faculty of Pharmacy, Universitas Gadjah Mada
Mufrod, Mufrod
Faculty of Pharmacy, Universitas Gadjah Mada
Stelechocarpus burahol (BL) Hook f. & Th., cream, antioxidant, physical properties
This research was aimed to determine the effect of concentrations of Kepel leaves’ (Stelechocarpus burahol (BL) Hook f. & Th.)extract to antioxidant activity and physical properties of cream. Kepel leaves’ extract were made by infundation method. The antioxidant activity was tested by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical scavenging method. Cream was made in three formulas with variation concentrations of Kepel leaves’ extract (2,5; 5,0; 7,5%b/b) using w/o basis. Physical stability parameters tested in this research were homogenity, dispersive power, adhesion, and viscosity. Data were then analyzed statistically by ANOVA One Way and Turkey Test at 95% level of significance. The results showed that concentration of Kepel leaves’ extract as an active ingredient cause different color, odor, and viscosity of the cream. The concentrationdifference of Kepel Leaves’ extract as an active ingredient was not affected the homogenity, adhesion, and the separation ratio of the cream. The difference concentration was not cause affected daya sebar cream unless the formula II (5.0% w/w) and formula III (7.5% w/w). Increasing concentration of Kepel leaves’ extract caused a different antioxidant activity unless the formula II (5.0% w/w) and formula III (7.5% w/w).
Faculty of Pharmacy, Universitas Gadjah Mada
2015-12-14 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8004
Majalah Obat Tradisional; Vol 17, No 2 (2012)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8005
2015-12-14T09:25:14Z
TradMedJ:ART
"151214 2015 eng "
2406-9086
1410-5918
dc
SYRUP FORMULATION OF EXTRACT OF DAUN LEGUNDI (Vitex trifolia L.)
Lisprayatna, Lalita
Faculty of Pharmacy, Universitas Gadjah Mada
Murti, Yosi Bayu
Faculty of Pharmacy, Universitas Gadjah Mada
Sulaiman, T. Nanda Saifullah
Faculty of Pharmacy, Universitas Gadjah Mada
Vitex trifolia. Leaves, viteksikarpin, propylene glycol
Daun legundi (Vitex trifolia) has been used by Indonesian people as antiasthma. This research aims to produced the good quality syrup of extract of daun legundi and also to evaluate the relative level change of viteksikarpin in syrup after formulation. Syrup of extract of daun legundi was made for 4 formulas of variation of propylene glycol (PG) level. Formula 1 (PG 11%), formula 2(PG 12%), formula 3 (PG 13%) and control (without PG). Each formula was tested for physical characteristic (organoleptic, viscosity, time of flow, flavor test), relative level of viteksikarpin against extract. The result showed that increase of propylene glycol level was increased the viscosity syrup and time of flow while the percentage of relative level of viteksikarpin against extract was decreased. In this research, formula 1 (PG 11%) was the selected formula because it has a good flavor and appearance, less viscosity, least precipitate, highest relative level of viteksikarpin and acceptable in the market.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-12-14 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8005
Majalah Obat Tradisional; Vol 17, No 2 (2012)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8009
2015-12-14T09:26:34Z
TradMedJ:ART
"151214 2015 eng "
2406-9086
1410-5918
dc
FORMULA OPTIMIZATION OF LOZENGES CONTAINING Alpinia galanga (L.) Stuntz RHIZOME EXTRACT WITH A COMBINATION OF MANNITOL AND SUCROSE SWEETENERS USING SIMPLEX LATTICE DESIGN METHOD
Aufiya, Duhita
Faculty of Pharmacy, Universitas Gadjah Mada
Pramono, Suwijiyo
Faculty of Pharmacy, Universitas Gadjah Mada
Mufrod, Mufrod
Faculty of Pharmacy, Universitas Gadjah Mada
galangal, lozenges, mannitol, sucrose, simplex lattice design
Galangal (Alpinia galanga (L.) Stuntz) has many benefits for the health. Galangal is used as a tonic traditionally, but the dose is unknown, so it is necessary to be formulated. Lozenges was selected for the formulation of galangal because its practical and comfortable form. Taste is an important parameter for the lozenges so a mixture of mannitol and sucrose are used in this formulation. This research aims to optimize the lozenges formulation using a mixture of mannitol and sucrose with Simplex Lattice Design method. Galangal rhizome was extracted by maceration using 70% ethanol. Lozenges were made in three formulas, Formula A (100%: 0%), Formula B (50%: 50%), and Formula C (0%: 100%) with SLD in wet granulation method. Granule mass was sieved through the sieve no. 10 and dried in an oven at 60°C, then dried granule was mixed homogeneously with magnesium stearate-talk and tested for its physical properties include flow rate and compactibility. The granule was compressed into tablets and tested for its physical properties, including weight uniformity, hardness, friability, dissolving time, and tasting test. The data which was obtained were used to create a profile of physical properties of the granule and the taste of the tablet. Optimum formula was selected based on the highest total response. The tablet was created based on the optimum formula and tested physical properties of granule and tablets. The data was obtained and compared theoritically and statistically using the T-Test. The result showed that optimum formula of galangal rhizome extract lozenges is a formula with ratio 25% maintol : 75% sucrose. The optimum formula of the flow rate and granule compactibility and significantly different between verification and SLD calculations, while the dissolved time and the respon of tablets flavor are not significantly different. The dominant composition of sucrose can increase granule's compactibility response, dissolving time, and tasting test of lozenges, lowering the response speed of the flow of granules.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-12-14 16:26:34
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8009
Majalah Obat Tradisional; Vol 17, No 3 (2012)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8010
2015-12-14T09:26:34Z
TradMedJ:ART
"151214 2015 eng "
2406-9086
1410-5918
dc
LOZENGES FORMULATION OFTURMERIC EXTRACT (Curcuma domestica) WITH COMBINATION OF FILLER AGENTS MANITOL – AMYLUM MANIHOT
Haryanti, Farida
Faculty of Pharmacy, Universitas Gadjah Mada
Purwantini, Indah
Faculty of Pharmacy, Universitas Gadjah Mada
Sulaiman, T. Nanda Saifullah
Faculty of Pharmacy, Universitas Gadjah Mada
turmeric rhizome, filler agents, simplex lattice design, lozenges
Hydroalcoholic extract of turmeric is recommended as supplement to prevent atherosclerosis, but the common dosage form in traditional practices is less practical and unacceptable. It is therefore recommended to be mad into new dosage form, lozenges. This research aims to determine the effect of filler agent manitol-amylum manihot variations to the physical characteristics of granule, tablet, and the flavor of the tablet. The optimum formula was evaluated with simplex lattice design method. The hydro extract of turmeric was made using aquadest 80OC while the alcoholic extract using ethanol 96%. Hydro alcoholic extract was made by mixing both extracts, wet granulated and formulated into five formulas with combination of manitol-amylum manihot ratio. The result showed that the combination of manitol-amylum manihot had affected the tablet hardness, solubility time and flavor acceptance. The formula with the combination manitol-amylum manihot 80% : 20% was found to be the optimum formula.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-12-14 16:26:34
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8010
Majalah Obat Tradisional; Vol 17, No 3 (2012)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8011
2015-12-14T09:26:34Z
TradMedJ:ART
"151214 2015 eng "
2406-9086
1410-5918
dc
ANTIOXIDANT ACTIVITY ANALYSIS OF Cynara scolimus L., Artemisia china L., Borreria repensDC.,Polygala paniculata L. FROM TAMAN NASIONAL GUNUNG MERAPI USING DPPH (2,2-DIFENIL-1-PIKRILHIDRAZIL) RADICAL SCAVENGING ANALYSIS
Murwanto, Paulus Eko
Faculty of Pharmacy, Universitas Gadjah Mada
Santosa, Djoko
Faculty of Pharmacy, Universitas Gadjah Mada
Cynara scolimus L., Artemisia china L., Borreria repens DC., Polygala paniculata L. DPPH, antioxidant
Taman Nasional Gunung Merapi (TNGM) has various biodiversities which need to be explored. This study aims to analyze antioxidant activity four plants from Asteraceae, Rubiaceae and Polygalaceae which were collected from TNGM. Antioxidant activity is analyzed by DPPH radical capturing method using spectrophotometer. Sampel is extracted gradually by wasbenzen and methanol. DPPH radical capturing analysis shows that IC50 score of Cynara scolimus L. wasbenzen extract is 496,16g/mL; Artemisia china L. 407,86g/mL; Borreria repens DC. 984,04 g/mL and Polygala paniculata L. 633,34 g/mL. The IC50 score of methanol extract Cynara scolimus L. is 334,94g/mL; Artemisia china L. 199,99g/mL; Borreria repens DC. 402,28 g/mL and Polygala paniculata L. 77,00 g/mL . Thin layer chromatography analysis shows that methanol extract of Polygala paniculata L. contains flavonoid.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-12-14 16:26:34
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8011
Majalah Obat Tradisional; Vol 17, No 3 (2012)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8012
2017-01-24T07:38:19Z
TradMedJ:ART
oai:jurnal.ugm.ac.id:article/8013
2015-12-14T09:26:34Z
TradMedJ:ART
"170209 2017 eng "
2406-9086
1410-5918
dc
LOZENGES FORMULATION OFTURMERIC EXTRACT (Curcuma domestica) WITH COMBINATION OF FILLER AGENTS MANITOL – AMYLUM MANIHOT
Sulaiman, T. Nanda Saifullah
Faculty of Pharmacy, Universitas Gadjah Mada
Purwantini, Indah
Faculty of Pharmacy, Universitas Gadjah Mada
Haryanti, Farida
Faculty of Pharmacy, Universitas Gadjah Mada
turmeric rhizome, filler agents, simplex lattice design, lozenges
Hydroalcoholic extract of turmeric is recommended as supplement to prevent atherosclerosis, but the common dosage form in traditional practices is less practical and unacceptable. It is therefore recommended to be mad into new dosage form, lozenges. This research aims to determine the effect of filler agent manitol-amylum manihot variations to the physical characteristics of granule, tablet, and the flavor of the tablet. The optimum formula was evaluated with simplex lattice design method. The hydro extract of turmeric was made using aquadest 80OC while the alcoholic extract using ethanol 96%. Hydro alcoholic extract was made by mixing both extracts, wet granulated and formulated into five formulas with combination of manitol-amylum manihot ratio. The result showed that the combination of manitol-amylum manihot had affected the tablet hardness, solubility time and flavor acceptance. The formula with the combination manitol-amylum manihot 80% : 20% was found to be the optimum formula.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-12-14 16:26:34
https://journal.ugm.ac.id/TradMedJ/article/view/8013
Majalah Obat Tradisional; Vol 17, No 3 (2012)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8014
2015-11-18T03:23:01Z
TradMedJ:ART
"160713 2016 eng "
2406-9086
1410-5918
dc
ANTIOXIDANT ACTIVITY ANALYSIS OF Cynara scolimus L., Artemisia china L., Borreria repensDC.,Polygala paniculata L. FROM TAMAN NASIONAL GUNUNG MERAPI USING DPPH (2,2-DIFENIL-1-PIKRILHIDRAZIL) RADICAL SCAVENGING ANALYSIS
Santosa, Djoko
Faculty of Pharmacy, Universitas Gadjah Mada
Murwanto, Paulus Eko
Faculty of Pharmacy, Universitas Gadjah Mada
Cynara scolimus L., Artemisia china L., Borreria repens DC., Polygala paniculata L. DPPH, antioxidant
Taman Nasional Gunung Merapi (TNGM) has various biodiversities which need to be explored. This study aims to analyze antioxidant activity four plants from Asteraceae, Rubiaceae and Polygalaceae which were collected from TNGM. Antioxidant activity is analyzed by DPPH radical capturing method using spectrophotometer. Sampel is extracted gradually by wasbenzen and methanol. DPPH radical capturing analysis shows that IC50 score of Cynara scolimus L. wasbenzen extract is 496,16g/mL; Artemisia china L. 407,86g/mL; Borreria repens DC. 984,04 g/mL and Polygala paniculata L. 633,34 g/mL. The IC50 score of methanol extract Cynara scolimus L. is 334,94g/mL; Artemisia china L. 199,99g/mL; Borreria repens DC. 402,28 g/mL and Polygala paniculata L. 77,00 g/mL . Thin layer chromatography analysis shows that methanol extract of Polygala paniculata L. contains flavonoid.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-18 10:23:01
https://journal.ugm.ac.id/TradMedJ/article/view/8014
Majalah Obat Tradisional; Vol 20, No 1 (2015)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8015
2015-12-07T08:42:24Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
ANTIBACTERIAL COMPOUNDS PRESENT ON n-HEXANE EXTRACT OF TEMPUYUNG LEAVES ( Sonchus arvensis L.)
Sukadana, I Made
Department of Chemistry, Faculty of Mathematics and Science, University of Udayana
Santi, Sri Rahayu
Department of Chemistry, Faculty of Mathematics and Science, University of Udayana
Sonchus Arvensis L., antibacterial, isolation, identification
Isolation of antibacterial compounds from n-hexane extract of tempuyung leaf (Sonchus Arvensis L.) has been carried out. n-hexane extract (9,15 g) showed antibacterial inhibition zone of 9 mm on Staphylococcus aureus and 8 mm on Escherichia coli at concentration of 1000 ppm. Fractionation of n-hexane extract using column chromatography resulted on 5 fractions (F1, F2, F3, F4, and F5). Antibacterial test of the fractions toward Staphylococcus aureus and Escherichia coli showed that fraction F2 and F4 positive toward Staphylococcus aureus and Escherichia coli. Fraction F3 showed just positive toward Escherichia coli while fraction F5 just positive toward Staphylococcus aureus. Further separation using preparative thin layer chromatography (TLC) technical resulted in 3 fractions (F4.1, F4.2 and F4.3 fractions), in which F4.1 fraction indicates the most active inhibition growth test again bacterial with diametric inhibition zone of 9 mm towards Staphylococcus aureus and 11 mm for Escherichia coli within concentration of 100 ppm. Gas Chromatography–Mass Spectroscopy (GC-MS) was employed in order to identity the F4.1 fraction. From WILEY7 database, it was identified that isolate are mixture of 2 compound components namely bis (2-ethylhexyl) ester and possibility of new compound included triterpenoid group with molecule formula C32H66O6.
Faculty of Pharmacy, Universitas Gadjah Mada
2011-01-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8015
Majalah Obat Tradisional; Vol 16, No 1 (2011)
eng
Copyright (c) 2011 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8016
2015-12-07T08:42:24Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
THE EFFECT OF CAULIFLOWER (Brassica oleracea L. var botrytis L.) JUICE PRETREATMENT ON DICLOFENAC ACTIVITY IN INFLAMATION THERAPY
Sunarsih, Endang Sri
Faculty of Medicine, University of Diponegoro
Palupi, Dwi Hadi Setya
Sekolah Tinggi Ilmu Farmasi "Yayasan Pharmasi"
Hapsari, Indriati
Sekolah Tinggi Ilmu Farmasi "Yayasan Pharmasi"
cauliflower juice, inflammation, diclofenac, rat
A drug therapy together with food consumption could influence the drug effectiveness. This research aimed to know the influence of cauliflower (Brassica oleracea L. var botrytis L.) juice pretreatment on diclofenac activity in inflammation therapy to wistar white male rats. Thirty rats divided into 6 groups randomized design. Group I (negative control) was only given aquadest, while group II (positive control) was given treatment with sodium diclofenac orally and the dose was 5,04 mg/kg BW. Group III was given pretreatment cauliflower juice with the dose of 20 ml/kg BW. It was given one hour before treatment with sodium diclofenac. Group IV was given pretreatment cauliflower juice with the dose of 20 ml/kg BW and it was given one day before treatment with sodium diclofenac. Group V was given pretreatment cauliflower juice with single dose 20 ml/kg BW for 3 days before treatment with sodium diclofenac, and group VI was given pretreatment cauliflower juice with single dose 20 ml/kg BW for 5 days before treatment with sodium diclofenac. Thirty minutes after treatment with sodium diclofenac, the rat’s paw was injected with 1% carrageenan 0,10 ml subplantary. Edema volume was measured from 0th hour to 5th hour after carrageenan injection used the pletismometer. The result of statistic analysis of AUC and DAI data have shown that cauliflower juice pretreatment given one hour before treatment with sodium diclofenac did not show significant different with positive control. The research showed that cauliflower juice pretreatment for 3 and 5 days was capable to reduce antiinflamation activity of diclofenac, therefore inflammation therapy with diclofenac was not maximum.
Faculty of Pharmacy, Universitas Gadjah Mada
2011-01-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8016
Majalah Obat Tradisional; Vol 16, No 1 (2011)
eng
Copyright (c) 2011 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8017
2015-12-07T08:42:24Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
CYTOTOXICITY, ANTIPROLIFERATIVE ASSAYS, AND EXPRESION OF P53 AND BCl2 OF ETHANOLIC FRACTION FROM TEA (Camellia sinensis (L.) O.K.) LEAVES INFUSE TO HeLa CELLS
Nurani, Laela Hayu
Faculty of Pharmacy, University of Ahmad Dahlan
Camellia sinensis, HeLa, p53, bcl-2
Tea (Camellia sinensis (L.) O.K.) is one of medical plant traditionally used by society as anticancer. The aim of this research is to evaluate the citotoxic and antiproliferative effect of ethanolic fraction of tea (Camellia sinensis (L.) O.K.) and its effect on P53 and bcl-2. Tea were collected and extracted by infundation and fractioned with ethanol as solvent. Citotoxicity test was done by incubating HeLa cell at a density of 2.104 with treatment using extract in concentration of 250; 125 ; 62,5; 31,25; 15,63; and 7,81 µg/mL during 24 hours. Antiproliferative test were done by calculating doubling time which was equaling a life cell on treatment sample concentration 31,25 µg/mL; 15,63 µg/mL; 7,81 µg/mL; and 3,91 µg/mL with cell control at 24, 48 and 72 hours. Imunohistochemistry assay was used in LC50 with p53 and bcl-2 antibody. P53 and bcl-2 expression is compared to control. The result indicated that ethanol fraction of tea (Camellia sinensis (L.) O.K.) leaf infuse has citotoxicity effect on HeLa cell with LC50 of 24,45 µg/mL. The result of doubling time test indicated that doubling time value for a solvent control is 74,11 hours and a cell control is 78,22 hours. While, with treatment 31,25 µg/mL; 15,63 µg/mL; 7,81 µg/mL; and 3,91 µg/mL resulted on negative slope then did not produce doubling time. The extract can induce p53 expression and inhibit bcl-2 expression compared to control.
Faculty of Pharmacy, Universitas Gadjah Mada
2011-01-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8017
Majalah Obat Tradisional; Vol 16, No 1 (2011)
eng
Copyright (c) 2011 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8018
2015-12-07T08:42:24Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
ANTIOXIDANT SCREENING ACTIVITY OF SEVERAL INDONESIAN MEDICINAL PLANTS USING 2,2-DIFENIL 1-1 PICRYLHIDRAZYL(DPPH)
Wulansari, Dewi
Center of Biological Research, Indonesian Institute of Science
Chairul, Chairul
Center of Biological Research, Indonesian Institute of Science
Antioxidant, medicinal plant, free radical, DPPH
Antioxidant is a compound useful to prevent oxidative damage generated by free radical in human body, thus protecting body from many diseases. The aim of this research is to investigate antioxidant activity of some Indonesian medicinal plants. A total of 37 methanolic extracts of Indonesian plants have been screened for their antioxidant activity by in vitro method using the free radical 2,2-diphenyl-1-picrylhydrazyl (DPPH). Vitamin C was used as positive control. Of the samples investigated, 29 exhibited radical scavenging properties more than 50% at 1000 ppm. Five extracts exhibited high activities i.e. bark extracts of Sapium baccatum and Leucosyke capitellata, leaves extracts of Ardisia crispa, Glochidion cauliflorum, and Glochidion superbum.
Faculty of Pharmacy, Universitas Gadjah Mada
2011-01-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8018
Majalah Obat Tradisional; Vol 16, No 1 (2011)
eng
Copyright (c) 2011 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8019
2015-12-07T08:42:24Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
EXAMINATION OF ANALGETICS EFFECT OF EXTRACT BOESENBERGIA PANDURATA(Roxb.) SCHLECHTER TO SWISS FURROW MALE MICE
Winarti, Lina
Department of Pharmacy, University of Jember
Wantiyah, Wantiyah
Department of Pharmacy, University of Jember
analgetic activity examination, Boesenbergia pandurata (Roxb.) Schlechter extract, amount of writhing, protection percentage, analgetic efectivity percentage
This research is conducted to evaluate analgetics effect of Boesenbergia pandurata (Roxb.) Schlechter extract using Witkin et al method. This method is conducted to see the elimination effect of pain after the administration of acetate acid by intraperitoneal injection to swiss furrow male mice. Pain effect by the administration of acetate acid causing stomach wall contraction, till the head and feet pulled rear and abdomen touch the cage room base. This symptom is named by writhing reflex and this symptom can be eliminated with an analgesic. Analysis was done by comparing the amount of writhe that happened after the giving of Boesenbergia pandurata (Roxb.) Schlechter extract with acetosal as positive comparator and aquadest as negative control. Writhing effect calculated during 30 minute after the administration of acetate acid by intraperitoneal injection. Boesenbergia pandurata (Roxb.) Schlechter extract between dose 15; 30 and 60 mg / kgBW have significant difference ( P<0.05). In the dose-effect relation it can be seen that more higher the dose give more higher effect. The amount of writhing in Boesenbergia pandurata (Roxb.) Schlechter extract dose 30 mg / kgBW not significantly differ with Acetosal, while Boesenbergia pandurata (Roxb.) Schlechter extract dose 15 mg / kgBW and 60 mg / kgBW have significant difference with Acetosal. Boesenbergia pandurata (Roxb.) Schlechter extract dose 15 mg / kgBB showing the amount of writhing larger than Acetosal and Boesenbergia pandurata (Roxb.) Schlechter extract dose 60 mg / kgBB has amount of writhing fewer than Acetosal. From the calculation of protection percentage administration significant difference between group. Boesenbergia pandurata (Roxb.) Schlechter extract dose 30 mg / kgBW and 60 mg / kgBW do not show significant difference with Acetosal. While from effectiveness percentage analysis also showing significant difference between group except Boesenbergia pandurata (Roxb.) Schlechter extract dose 30 mg / kgBW with Acetosal 130 mg / kgBW. So from overall of analysis resulting that analgetics activity of Boesenbergia pandurata (Roxb.) Schlechter extract start from dose 30 mg / kgBW have effective effect to reduce the pain induced with acetatic acid which equivalent with Acetosal as positive control. More great the dose give higher activity. From this research the dose of Boesenbergia pandurata (Roxb.) Schlechter extract 60 mg / kgBW have analgetics activity stronger than Acetosal
Faculty of Pharmacy, Universitas Gadjah Mada
2011-01-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8019
Majalah Obat Tradisional; Vol 16, No 1 (2011)
eng
Copyright (c) 2011 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8020
2015-12-07T08:43:28Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
ANTI-INFLAMMATORY ACTIVITYY OF Piper crocatum Ruiz & Pav. LEAVES METANOLIC EXTRACT IN RATS
Fitriyani, Atik
Faculty of Pharmacy, University of Jember
Winarti, Lina
Faculty of Pharmacy, University of Jember
Muslichah, Siti
Faculty of Pharmacy, University of Jember
Nuri, Nuri
Faculty of Pharmacy, University of Jember
extract P.Crocatum, anti-inflammatory effect, Acetosal
Inflammation is a natural respon for tissue damage. To reduce inflammation, people used a NSAID (Non Steroid Antiinflammatory Drugs). This agent caused many side effects. One of medicinal plant empirically used for traditional medicine is Piper Crocatum Ruiz & Pav. This plant is potential to be developed as medicine for anti-inflammatory because its contains flavonoid, saponin, tannin, and alkaloid. The aim of this study was to investigate the anti-inflammatory effect of the P.Crocatum extract using carrageenan-induced rat oedema test. For the anti-inflammatory activity measurement, five different groups were established and piper extract was administered in three different doses : 25, 50 and 100 mg/kgBW. Acetosal was used as a reference agent. It was found that reduction in the flammation was 77.58% for acetosal, 72.37% for 25 mg/kg extract, 85.60% for 50mg/kg, and 81.02% mg/kg for 100mg/kg extract. Extract of P. Crocatum at 50 mg/kg showed the strongest anti-inflammatory activity among the doses used. The results showed that P. Crocatum extract posseses promising anti-inflammatory effect.
Faculty of Pharmacy, Universitas Gadjah Mada
2011-01-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8020
Majalah Obat Tradisional; Vol 16, No 1 (2011)
eng
Copyright (c) 2011 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8033
2015-11-24T05:21:55Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
INFLUENCE OF TEA LEAVES Camelia sinensis L. PHENOLIC CONTENT AS PREFERENCE FACTOR OF EMPOASCA SP. (Homoptera : Cicadellidae)
Saiful, Saiful
Faculty of Agricultural, Universitas Muhammadiyah Palu
Sudarsono, Sudarsono
Faculty of Pharmacy, Universitas Gadjah Mada
total phenol content, repellent, detterent, Empoasca sp.
Golongan senyawa fenolik adalah metabolit sekunder yang terdapat di seluruh bagian tumbuhan yaitu buah, kulit, akar, batang dan daun tumbuhan berfungsi sebagai pertahanan kimia (chemical defence) bersifat repellent dan detterent terhadap serangga herbivora. Golongan flavonoid lainnya yaitu katekin. Katekin dan turunannya (gallokatekin) pada daun teh diketahui berfungsi sebagai mekanisme ketahanan terhadap Empoasca sp. (Homoptera: cicadellidae). Untuk mengetahui kadar fenol pada tanaman teh Camelia sinensis L. klon Kiara dan klon Pasir Sarongge (PS) serta pengaruh golongan senyawa fenolik terhadap preferensi Empoasca sp. Hasil penelitian menunjukkan semakin tinggi polifenol pada pucuk daun teh semakin tidak disukai Empoasca sp.
Faculty of Pharmacy, Universitas Gadjah Mada
2013-05-01 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8033
Majalah Obat Tradisional; Vol 18, No 2 (2013)
ind
Copyright (c) 2013 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8036
2015-11-24T05:21:55Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
MOUTHWASH FORMULATION OF BASIL OIL (Ocimum basilicum L.) AND IN VITRO ANTIBACTERIAL AND ANTIBIOFILM ACTIVITIES AGAINST Streptococcus mutans
Yosephine, Ardiana Dewi
Faculty of Pharmacy, Universitas Gadjah Mada
Wulanjati, Martha Purnami
Faculty of Pharmacy, Universitas Gadjah Mada
Saifullah, Teuku Nanda
Faculty of Pharmacy, Universitas Gadjah Mada
Astuti, Puji
Faculty of Pharmacy, Universitas Gadjah Mada
mouthwash, antibiofilm, Streptococcus mutans, Ocimum basilicum L.
Kemangi (Ocimum basilicum L.) mengandung minyak atsiri yang dilaporkan memiliki aktivitas antibakteri. Berdasarkan sifat antibakterinya, maka minyak atsiri daun kemangi dapat dikembangkan menjadi mouthwash untuk mencegah timbulnya plak gigi. Penelitian ini bertujuan untuk mengetahui pengaruh pemberian tween 80 (emulgator) dan gliserin (stabilizer) pada sifat fisik mouthwash, mengetahui perbandingan tween 80 dan gliserin yang menghasilkan mouthwash dengan stabilitas terbaik, serta melihat aktivitasnya sebagai agen antibakteri dan antibiofilm terhadap Streptococcus mutans secara in vitro. Minyak atsiri diperoleh dengan cara distilasi air dan uap air. Minyak atsiri kemudian diformulasi menjadi sediaan mouthwash dan dilakukan variasi jumlah tween 80 serta gliserin untuk mengetahui pengaruhnya terhadap sifat fisik sediaan. Aktivitas antibakteri dan antibiofilm diuji dengan metode mikrodilusi. Hasil penelitian menunjukkan bahwa tween 80 berpengaruh signifikan terhadap kenaikan viskositas dan gliserin berpengaruh signifikan terhadap kenaikan massa jenis bila keduanya ditambahkan lebih dari 2,5 mL dalam 50 mL mouthwash. Dari lima formula yang dibuat, formula dengan perbandingan tween 80 dan gliserin = 3,75 mL : 1,25 mL ditemukan sebagai formula terbaik. Mouthwash daun kemangi menunjukkan aktivitas antibakteri dan antibiofilm terhadap Streptococcus mutans secara in vitro. Sediaan tersebut memiliki nilai KHM sebesar 0,1 % v/v dengan % penghambatan pertumbuhan bakteri sebesar 87,50 ± 3,33 %, sedangkan kadar optimum minyak atsiri daun kemangi yang mampu menghambat serta mendegradasi biofilm berturut-turut adalah 0,1 % v/v dan 0,2 % v/v dengan % penghambatan dan degradasi biofilm sebesar 77,52 ± 0,82 % dan 57,64 ± 6,09 %.
Faculty of Pharmacy, Universitas Gadjah Mada
2013-05-01 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8036
Majalah Obat Tradisional; Vol 18, No 2 (2013)
ind
Copyright (c) 2013 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8038
2015-11-24T05:21:55Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
FORMULATION LAZONGE OF GUAVA LEAVES (Psidium guava L.) CONTAINING FLAVONOIDS WITH A COMBINATION OF EXCIPIENTS MANNITOL – SUCROSE
Juliantoni, Yohanes
Faculty of Pharmacy, Universitas Gadjah Mada
Mufrod, Mufrod
Faculty of Pharmacy, Universitas Gadjah Mada
Lozenges, guava leaf, manitol, sucrose
Senyawa flafonoid yang terdapat pada daun jambu biji diketahui memiliki aktivitas sebagai antibakteri. Penelitian ini bertujuan untuk memformulasikan ekstrak daun jambu biji menjadi sediaan yang acceptable. Eksrak daun jambu buji diformulasikan menjadi sediaan tablet hisap dengan kombinasi bahan pengisi manitol-sukrosa. Metode Simple Lattice Desaign digunakan untuk mengetahui pengaruh kombinasi bahan terhadap sifat fisik granul dan tablet dan prediksi formula optimal. Ekstrak dibuat dengan cara maserasi simplisia menggunakan etanol 70%, dikentalkan dan dikeringkan menggunakan alat freeze dryer. Tablet hisap dibuat dalam 5 formula berdasarkan kombinasi monitol-sukrosa yaitu: F1(0%:100%) ; F2(25%:75%); F3(50%:50%); F4(75%:25%); F5(25%:75%) menggunakan bahan pengikat larutan gelatin 5%. Granul kering yang dihasilkan diuji sifat alirnya, dicampur homogenya dengan lubrikan dan dikempa. Tablet yang dihasilkan diuji keseragaman bobot, kekerasan tablet, kerapuhan dan tanggapan rasa. Persamaan Simple lattice desaign dan formula optimum diprediksi menggunakan software Desaign Expert versi 8.0.5.2. Tablet hisap masing-masing formula yang dihasilkan telah memenuhi syarat beberapa sifat fisik tablet hisap. Perubahan proporsi kombinasi manitol-sukrosa mempengaruhi sifat fisik granul maupun tablet. Berdasarkan analisis menggunakan software Desaign Expert versi 8.0.5.2, formula optimal diberikan oleh kombinasi manitol-sukrosa 82,28%:17.72%.
Faculty of Pharmacy, Universitas Gadjah Mada
2013-05-01 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8038
Majalah Obat Tradisional; Vol 18, No 2 (2013)
ind
Copyright (c) 2013 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8041
2015-11-24T05:21:55Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
PHYSICAL STABILITY AND ACTIVITY OF CREAM W/O ETANOLIK FRUIT EXTRACT MAHKOTA DEWA (Phaleria macrocarph (scheff.) Boerl,) AS A SUNSCREEN
Shovyana, Hidayatu Hana
Faculty of Pharmacy, Universitas Gadjah Mada
Zulkarnain, A. Karim
Faculty of Pharmacy, Universitas Gadjah Mada
Mahkota dewa, cream, sun screen
Krim ekstrak etanolik buah mahkota dewa (Phaleria macrocarph (scheff.) Boerl,) telah diuji aktivitasnya sebagai tabir surya secara in vivo. Penelitian ini bertujuan untuk mengetahui aktivitas tabir surya dan stabilitas fisiknya. Penentuan aktivitas tabir surya dilakukan dengan menentukan nilai SPF (Sun Protection Factor) secara ini vivo pada mencit galur Swiss Weber. Uji ini digunakan 1 kelompok tanpa perlakuan, 1 kelompok control negatif (basis), dan 4 kelompok perlakuan. Kelompok perlakuan adalah krim w/o ekstrak etanolik buah mahkota dewa. Nilai SPF merupakan perbandingan DEM pada kulit yang diberi krim tabir surya dengan DEM pada kulit tanpa krim. Uji stabilitas fisik krim meliputi uji viskositas, daya sebar, daya lekat, dan rasio pemisahan. Data dianalisis menggunakan SPSS untuk mengetahuipengaruh penambahan ekstrak dan lama penyimpanan terhadap kestabilan krim. Hasil penelitian menunjukkan bahwa krim mahkota dewa kadar 4,6,d dan 10% memiliki aktivitas sebagai tabir surya dengan nilai SPF sebesar 1,25; 1,56; 2,4; dan 3,05. Selain itu mahkota dewa memiliki stabilitas fisik yang baik selama penyimpanan.
Faculty of Pharmacy, Universitas Gadjah Mada
2013-05-01 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8041
Majalah Obat Tradisional; Vol 18, No 2 (2013)
ind
Copyright (c) 2013 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8043
2015-11-24T05:21:55Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
DETERMINATION OF ALKALOID CONTENT OF ETHANOLIC EXTRACT OF Hibiscus rosa-sinensis L. FLOWER
Murrukmihadi, Mimiek
Faculty of Pharmacy, Universitas Gadjah Mada
Wahyuono, Subagus
Faculty of Pharmacy, Universitas Gadjah Mada
Marchaban, Marchaban
Faculty of Pharmacy, Universitas Gadjah Mada
Martono, Sudibyo
Faculty of Pharmacy, Universitas Gadjah Mada
alkaloid, ethanolic extract, Hibiscus rosa-sinensis L.
Bunga kembang sepatu (Hibiscus rosa-sinensis L.) secara tradisional digunakan sebagai peluruh dahak. Berdasarkan atas Bioassay Guided Fractionation, fraksi aktif berhasil dipisahkan dan alkaloid merupakan kandungan utama fraksi. Oleh karena itu alkaloid digunakan sebagai senyawa penanda (marker) ekstrak etanol Hibiscus rosa-sinensis L. Nilai viskositas digunakan sebagai model untuk aktivitas peluruh dahak, dengan asetil sistein sebagai kontrol positif. Selanjutnya penetapan kadar alkaloid dalam ekstrak etanol dilakukan secara KLT-Densitometri (n=5), kadar alkaloid dibandingkan dengan kurva baku dari alkaloid (marker) hasil isolasi (Y=12,1360X+2901,4474). Kadar alkaloid dalam ekstrak etanol kembang sepatu (Hibiscus rosa-sinensis L.) sebagai 2,35 ± 0,67 %.
Faculty of Pharmacy, Universitas Gadjah Mada
2013-05-01 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8043
Majalah Obat Tradisional; Vol 18, No 2 (2013)
ind
Copyright (c) 2013 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8045
2015-11-24T05:21:55Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
ANTIBACTERIAL ACTIVITY ESSENTIALS OILS PONTIANAK ORANGE PEELS AGAINST Staphylococcus aureus and Escherichia coli
Sari, Rafika
Department of Pharmacy, Faculty of Medicine, Universitas Tanjungpura, Pontianak
Mustari, F. Nour Aulia
Department of Pharmacy, Faculty of Medicine, Universitas Tanjungpura, Pontianak
Wahdaningsih, Sri
Department of Pharmacy, Faculty of Medicine, Universitas Tanjungpura, Pontianak
antibacterial, essential oils, Pontianak oranges peels, S.aureus, E.coli
Penyakit infeksi dapat disebabkan oleh bakteri patogen diantaranya Staphylococcus aureus dan Escherichia coli. Salah satu tanaman yang dapat dimanfaatkan sebagai antibakteri adalah minyak atsiri kulit buah jeruk Pontianak (Citrus nobilis Lour. var. microcarpa). Penelitian ini bertujuan untuk mengetahui aktivitas antibakteri terhadap bakteri uji Staphylococcus aureus dan Escherichia coli dengan metode disc diffusion. Penelitian yang dilakukan bersifat eksperimental dengan menggunakan Rancangan Acak Lengkap (RAL) Faktorial kemudian hasil dianalisis menggunakan program CoStat metode Two Way ANOVA dan diuji lanjut menggunakan LSD (Least Significant Different). Hasil penelitian menunjukkan bahwa minyak atsiri kulit buah jeruk Pontianak mengandung flavonoid, saponin, dan triterpenoid. Berdasarkan hasil analisis, menunjukkan bahwa minyak atsiri dari kulit buah jeruk Pontianak memiliki aktivitas antibakteri terhadap bakteri Escherichia coli dan Staphylococcus aureus, dimana rata-rata zona hambat terhadap bakteri diperoleh dari konsentrasi 0,5; 1,5; dan 2,5 mg/mL secara berurutan 16,33; 18; dan 21 mm dan 15; 16, dan 19 mm. Konsentrasi terbaik yang memberikan zona hambat terbesar terhadap kedua jenis bakteri adalah konsentrasi 2,5 mg/mL.
Faculty of Pharmacy, Universitas Gadjah Mada
2013-05-01 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8045
Majalah Obat Tradisional; Vol 18, No 2 (2013)
ind
Copyright (c) 2013 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8047
2015-11-24T13:34:33Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
FLAVONOID AND TANNIN ANALYSIS WITH MICROSCOPY – MICROCHEMICAL METHOD
Mulyani, Sri
Faculty of Pharmacy, Universitas Gadjah Mada
Laksana, Toga
Faculty of Pharmacy, Universitas Gadjah Mada
analysis, flavonoids, tannins, microscopy-microchemical
Studies have been conducted to analyze flavonoids and tannins using microscopy-microchemical methods. Materials used for the analysis of flavonoid were orange peel, sonchi leaves, and orthosiphon leaves, with N. exaltata as negative control. While guajava leaves, guazama leaves, and areca seed were used for the analysis of tannins, with ginger rhizome as negative control. The study was conducted in a way of material sliced crosswise, subsequently given the identification reagent for flavonoids (ammonia, NaOH, AlCl3, sitroborat). On the other hand, using FeCl3, fast blue salt, and prusian blue for tannins. Having examined under a microscope, the area which gave a positive reaction to the reagent used, indicate the test materials contained compounds that were analyze. The results showed that this microscopy-microchemical method can be used to analyze the presence of flavonoids / tannins in the test material. Presence of flavonoid compounds will give yellow color with ammonia, NaOH, AlCl3, sitroborat reagent, while blue to black for the tannins with FeCl3, red with fast blue salt, and blue with prusian blue.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:34:33
https://journal.ugm.ac.id/TradMedJ/article/view/8047
Majalah Obat Tradisional; Vol 16, No 3 (2011)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8048
2015-11-24T13:34:33Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
ANALYSIS ANTIOXIDANT ACTIVITY AND TOTAL ANTHOCYANIN CONTENT IN EXTRACT AND LIPOSOME OF ROSELLE (Hibiscus Sabdariffa L.) CALYX
Juniarka, I Gede Agus
Faculty of Pharmacy, Universitas Gadjah Mada
Lukitaningsih, Endang
Faculty of Pharmacy, Universitas Gadjah Mada
Noegrohati, Sri
Faculty of Pharmacy, Universitas Gadjah Mada
rosella, liposome, stability, antioxidan anthocyanin
The form of liposome formulations is one of strategies to overcome the limitations of anthocyanin extract stability of roselle calyx (Hibiscus sabdariffa L.). In this study, antioxidant comparative analysis of antioxidant activity and total anthocyanin content from rosella extract and its liposomes form was conducted. Preparation of extract made by maceration using the best solvent consisted of methanol : formic acid (97:3) (v/v). From this procedure, the obtained extract was about 26.40% and antioxidant activity was 19.99%. Liposome formulations of methanol extract from roselle calyx were prepared by reverse phase method using rosella extract as aqueous phase and egg lecithin as the lipid phase. The stability of exctract and liposome form of rosella calyx was compared. The stability study showed that there was a reduction of antioxidant activity (DPPH test) 11.25 % for the roselle extract, while the liposome form was 0.93% after 30 days storage in low temperature (0-50C) and saturated nitrogen. In addition, total anthocyanin content (pH differential method test) decreased approximately 39.12 % for the roselle extract and 0.72% for the liposome form. More over, the liposome had lower level of irritation than the form of extract. Therefore, it can be concluded that anthocyanin encapsulated by liposome was more stable, safe, and effective than the anthocyanin of the rosella calyx extract.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:34:33
https://journal.ugm.ac.id/TradMedJ/article/view/8048
Majalah Obat Tradisional; Vol 16, No 3 (2011)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8049
2015-11-24T13:34:33Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
THE ANTIDIABETICS OF COMBINATION METFORMIN AND PURIFIED EXTRACT OF Andrographis paniculata (Burn).F.NESS IN HIGH FRUCTOSE-FAT FED RATS
Syamsul, Eka Siswanto
Faculty of Pharmacy, Universitas Gadjah Mada
Academy of Pharmacy Samarinda
Nugroho, Agung Endro
Faculty of Pharmacy, Universitas Gadjah Mada
Pramono, Suwijiyo
Faculty of Pharmacy, Universitas Gadjah Mada
A. paniculata, metformin, insulin resistance
Sambiloto (A. paniculata) merupakan tanaman yang dapat digunakan untuk mengatasi penyakit Diabetes Mellitus (DM). Dalam terapi DM terdapat kemungkinan pemakaian bersama-sama dengan Obat Hipoglikemik Oral (OHO), misalnya: metformin. Penelitian ini bertujuan untuk mengetahui potensi kombinasi ekstrak terpurifikasi herba sambiloto dan metformin terhadap peningkatan efek penurunan kadar glukosa darah pada tikus DM tipe 2 resisten insulin. Penelitian ini dilakukan menggunakan metode acak lengkap pola searah. Hewan uji yang digunakan dibagi 4 kelompok, kelompok 1: pemberian metformin 45 mg/kg BB (kontrol positif), kelompok 2: pemberian ekstrak terpurifikasi herba sambiloto 434,6 mg/kg BB, kelompok 3: pemberian kombinasi metformin 45 mg/kg BB dengan ekstrak 434,6 mg/kg BB (kombinasi 1), dan kelompok 4: pemberian metformin 22,5 mg/kg BB dengan ekstrak 434,6 mg/kg BB (kombinasi 2). Hewan uji DM tipe 2 resisten insulin dibuat dengan pemberian fruktosa 1,8 g/kg BB dan makanan kaya lemak selama 50 hari. Penetapan kadar glukosa darah menggunakan reagen kit. Pengamatan ekspresi GLUT-4 pada sel otot menggunakan teknik imunohistokimia. Resistensi insulin pada tikus diuji menggunakan 3 parameter, yaitu: (1) uji kadar glukosa darah preprandial dan postprandial; (2) aktivitas hipoglikemik glibenklamid; dan (3) pengamatan ekspresi protein GLUT-4 pada jaringan otot. Hasil menunjukkan bahwa hewan uji telah resisten insulin. Hasil uji aktivitas antidiabetes menunjukkan bahwa persen daya hipoglikemik kombinasi 1 dan 2 lebih rendah dibandingkan dengan pemberian metformin atau ekstrak terpurifikasi secara tunggal (p<0,05). Dari hasil di atas disimpulkan bahwa kombinasi ekstrak sambiloto terpurifikasi dengan metformin tidak meningkatkan potensi antidiabetes dari penggunaan tunggalnya.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:34:33
https://journal.ugm.ac.id/TradMedJ/article/view/8049
Majalah Obat Tradisional; Vol 16, No 3 (2011)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8050
2015-11-24T13:34:33Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
ANTIMICROBIAL ACTIVITY OF BIOACTIVE METABOLITES OF (Lentinula edodes (Berk.) Pegler) CULTURED ONTHREE TYPES OF FERMENTATION MEDIA
Ekowati, Nuraeni
Faculty of Biology, University of Jenderal Soedirman
Faculty of Biology, Universitas Gadjah Mada
Kasiamdari, Rina Sri
Faculty of Biology, Universitas Gadjah Mada
Pusposendjojo, Nursamsi
Faculty of Agriculture, Universitas Gadjah Mada
Soegihardjo, C.J.
Faculty of Pharmacy, University of Sanata Dharma
Lentinula edodes, fermentation medium, antimicrobial, bioactive metabolites
Shiitake mushroom (Lentinula edodes (Berk.) Pegler) is one of the fungi that has potentially been used as food and medicinal mushroom. Cultivation in liquid medium by the fermentation process has been developed over recent years. Four isolates of L.edodes (isolates from Malang, Cianjur, Lembang and Yogyakarta) cultured on three types of fermentation media (KM: Medium Kauffman; GYMT: Glucose, Yeast Extract, Malt Extract, Thiamin; YEMR: Yeast extract, Malt Extract, Rice bran). Bioactive metabolites extracted from mycelium biomass and fermentation culture filtrates were tested using pathogenic microbial Staphylococcus aureus ATCC 25923, Escherichia coli ATCC 35218, Candida albicans ATCC 10231, and Trichophyton mentagrophytes. Inhibition zone diameter data were analyzed using analysis of variance followed by Duncan test at 5% error rate. The results showed that bioactive compounds in the culture filtrates and mycelium biomass of L. edodes extracted using chloroform, ethyl acetate and water, were able to inhibit S. aureus, E. coli and C. albicans, but were not able to inhibit T.mentagrophytes. Isolates of L.edodes from Lembang and Yogyakarta cultured on Kauffman Medium gave the best results with inhibitory zones of 24.97 to 31.14 mm. Microbes which gave the most sensitive of bioactive compounds test from L. edodes were C. albicans followed by E. coli, S. aureus and T. mentagrophytes. Bioactive metabolites from L. edodes inhibited potentially pathogenic microbes from the group of bacteria and yeasts but did not inhibit the fungus.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:34:33
https://journal.ugm.ac.id/TradMedJ/article/view/8050
Majalah Obat Tradisional; Vol 16, No 3 (2011)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8051
2015-11-24T13:34:33Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
THE ACTIVITY OF RADICAL SCAVENGING OF 2,2-DIPHENYL-1-PYCRILHYDRAZIL BY ETHANOLIC EXTRACTS OF (Tinospora crispa (L.) Miers) STEM AND ITS FRACTIONS
Irianti, Tatang
Faculty of Pharmacy, Universitas Gadjah Mada
Puspitasari, Andayana
Faculty of Pharmacy, Universitas Gadjah Mada
Suryani, Ema
Faculty of Pharmacy, Universitas Gadjah Mada
radical scavenging, Tinospora crispa (L.) Miers, DPPH
We have investigated the antiradical activity of ethanolic extracts of Brotowali stem (Tinospora crispa (L.) Miers) using radical scavenging assay or DPPH radical. Thin layer chromatograms were also studied to estimate the group of compounds that have antiradical activity. The ethanolic extract, ethyl acetate and water fractions were showed coumarin and flavonoid. The DPPH antiradical efficiency values of ethyl acetate fraction was the highest antiradical activity namely 200 µg/ mL of this fraction was able to inhibit 53.57% DPPH radicals.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:34:33
https://journal.ugm.ac.id/TradMedJ/article/view/8051
Majalah Obat Tradisional; Vol 16, No 3 (2011)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8052
2015-11-24T13:34:33Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
THE ANTIINFLAMMATORY ACTIVITY OF HOT WATER EXTRACT OF CASHEW NUT’S STEM BARK (Anacardium occidentale L.) ON RAT PAW OEDEMA INDUCT BY CARRAGEENAN
Veriony, Lini
Faculty of Pharmacy, Universitas Gadjah Mada
Sudarsono, Sudarsono
Faculty of Pharmacy, Universitas Gadjah Mada
Nugroho, Agung Endro
Faculty of Pharmacy, Universitas Gadjah Mada
Cashew nut stem bark (Anacardium occidentale L.), anacardic acid, phenolic, hot water extract, anti-inflamatory
Hot water extract of cashew nut’s stem bark (Anacardium occcidentale L.) traditionally has been used as mouth washer to heal mouth and gum inflammation. Combination of aqueous extract of cashew nut stem bark and grape juice have anti-inflammatory activity. Cashew nut stem bark contains gallic acid and anacardic acid that had anti-inflammatory effect. The aim of this research is to observe anti-inflammatory activity of the hot water extract of Cashew nut’s stem bark compared to Indomethacin Non-Steroid Anti-inflammatory Drug. This research’s process consists of collection of the stem barks, extraction of the stem bark by hot water, qualitative analysis of gallic acid and anacardic acid, measurement total phenolic value, free radical scavenging and antiinflamatory activity assay. The hot water extract of stem bark was used to observe anti-inflammatory activity by 1.25; 2.5; 5g/kgBB and as positive control was indomethacin 10 mg/kgBB. The result of edema volume determination were counted as area under curve value and anti-inflammatory percentage and then the data were analyzed to evaluate the data difference of every group. The anti-inflammatory activity of 1.25; 2.5 and 5g/kgBB were not significantly different compared to 10 mg/kg BB indomethacin. The total phenolic concentration was gradually decreasing with the increasing concentration of the hot water extract of stem bark. The free radical scavenging DPPH activity at 1mg/mL hot water extract of stem bark was poorly than gallic acid 1mg/mL.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:34:33
https://journal.ugm.ac.id/TradMedJ/article/view/8052
Majalah Obat Tradisional; Vol 16, No 3 (2011)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8053
2015-11-24T13:34:33Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
FREE RADICAL SCAVENGING ACTIVITY OF (Alsophila glauca J. Sm)
Wahdaningsih, Sri
Faculty of Medicine and Health Science, University of Tanjungpura
Setyowati, Erna Prawita
Faculty of Pharmacy, Universitas Gadjah Mada
Wahyuono, Subagus
Faculty of Pharmacy, Universitas Gadjah Mada
fern stem, antioxidant, DPPH (2,2-Difenil-1 pikrilhidrazin)
Free radical induced oxidative stress that influences the occurrence of various degenerative diseases such as cancer, coronary heart disease and premature aging. In the case that body's antioxidant defense system does not have excessive antioxidants, additional natural antioxidant via food or other nutrients intake is needed. Stems of ferns are known to contain phenolic compounds that are known to have antioxidant activity. A study has been carried out to determine antioxidant potential of stems of ferns of western Borneo origin compared to quercetin as positive control. Initially, material was macerated gradually with wasbenzen followed by methanol. The extract obtained was filtered and evaporated to dryness to give dried wasbenzen extract and methanol extract respectively. Antioxidant properties of extracts were performed by DPPH on TLC. Active (wasbenzen) extract was then partitioned with 80% methanol to give soluble and insoluble fractions. Active (insoluble) fraction was further fractionated by vacuum liquid chromatography using a gradient mobile phase with increasing polarity (wasbezen: chloroform). Fractions obtained were tested antioxidant activity, and isolation was done by preparative TLC. The purity of isolated antioxidant compound was confirmed by TLC, and antioxidant potential was measured spectroscopically with DPPH method. This compound displayed antioxidant activity, but its value was lower than that of quercetin used as a positive control (IC50 178.4 v / s 2.17 microg / mL)
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:34:33
https://journal.ugm.ac.id/TradMedJ/article/view/8053
Majalah Obat Tradisional; Vol 16, No 3 (2011)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8054
2015-11-24T13:34:33Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
ISOLATION AND IDENTIFICATION OF ANTIOXIDANT COMPOUND OF PERSIMMON LEAVES (Diospyros kaki Thunb.) USING DPPH (2,2 DIPHENYL-1-PIKRILHIDRAZIL) METHOD
Isnindar, Isnindar
Faculty of Medicine and Nursing, University of Tanjungpura
Wahyuono, Subagus
Faculty of Pharmacy, Universitas Gadjah Mada
Setyowati, Erna Prawita
Faculty of Pharmacy, Universitas Gadjah Mada
Leaf persimmon (Diospyros kaki Thunb.), isolation, antioxidant, DPPH (2,2-diphenyl-1-pikrilhidrazil)
Antioxidant is a substance which in small concentrations can significantly inhibit or prevent the oxidation of the substrate. One plant is efficacious as an antioxidant is the persimmon (Diospyros kakiThunb.) where is cultivated widely in East Asia, Spain and Indonesia. In the search for natural antioxidant compounds, have been studied isolation and identification of antioxidant compound of persimmon leaves (DiospyroskakiThunb.) using DPPH (2,2-diphenyl-1-pikrilhidrazil) method. The extraction of persimmon leaves was carried out by maceration method with wasbenzen. Extracts obtained by evaporating the solvent wasbenzenon a rotary evaporator and were then re-macerated with methanol. Antioxidant activity of extracts was evaluated with DPPH 0.2% method. Active extract was partitioned with chloroform, methanol, distilled water and tested for antioxidant activity by DPPH 0.2% method. The active compound was purified byPTLC and the purified active isolates was then confirmed by TLC. The antioxidant activity (IC50) active isolates were analyzed using spectrophotometry. Analysisof active isolates resulted the antioxidant activity (IC50) of 100.00 ug / ml. UV-Vis spectrum of the active isolates showed absorption at λmax 285 and 401 nm. Infrared spectra (KBR) showed absorption at 3446 cm-1 (OH), 2926 cm-1 (CHaliphatic), 1456 cm-1 (CH2), 1384 cm-1 (CH3), 1255 cm-1, and 1115 cm-1 (C-0-C), 1631 cm-1 (C=Caromatic). GC-MS spectra gave two peaks with retention time 11.408 minutes with a similarity index of 79.243% with the molecular ion (M•)+ appeared at m/z 178, 163, 147. These spectra are expected to be methyl eugenol. Furthermore, the peak with retention time 12.982 minutes with a similarity index of 20.757%, producing a mass spectrum with molecular ion peaks (M•)+appeared at m/z 192, 177, 161. These spectra are expected to be myristicin compound.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:34:33
https://journal.ugm.ac.id/TradMedJ/article/view/8054
Majalah Obat Tradisional; Vol 16, No 3 (2011)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8055
2015-11-24T13:34:33Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
IDENTIFICATION OF COMPOUND ANTIFUNGI OF Sandoricum koetjape. Stem AND ACTIVITY TO Candida albicans
Warsinah, Warsinah
Faculty of Medicine and Health Science, University of Jenderal Soedirman
Kusumawati, Eka
Faculty of Medicine and Health Science, University of Jenderal Soedirman
Sunarto, Sunarto
Faculty of Medicine and Health Science, University of Jenderal Soedirman
Candidaalbicans, Fractionation, Identification, Sandoricumkoetjape
Kecapi (Sandoricum koetjape (Burm.f.) Merr) was one of the traditional medicine from family Meliaceae and had compounds such as flavonoids, saponins and polyphenols that can be used as antifungal. The objective of this research to know compounds contained from S. koetjape bark that has potential as an antifungal toward C. albicans.Research carried out two stages using extraction by maceration with methanol and fractionation by column chromatography method used the solvent n-hexane, chloroform, ethyl acetate and methanol. The next step was examined its activity toward C. albicans and identification of fraction active compounds with the Gas Chromatograpy-Mass Spectrometry (GC-MS). The results of this research showed that methanol extract from S. koetjape bark had antifungal activity againstC. albicans (39,654%). Next, the methanol extract was fractionated and fractionation results were obtained six fractions with the greatest antifungal activity of fraction III, followed by fraction V (39.66% b/v), fraction IV (39.02% b/v), fraction II (37.91% b/v), fraction VI (35.48 % b/v) and fraction I (33.72% b/v). Identification of compounds with GC-MS in fraction III and compounds contained have potential as an antifungal are α-gurjunene, trans-caryophyllene, aromadendrene, α-humulene, β-caryophyllene, δ-Cadinene, alloaromadendrene, octadecanoic acid (as. stearat), hexadecanoic acid metil ester (metil palmitate), hexadecanoic acid (as. palmitate), 9-octadecenoic acid metil ester (metil elaidate), 9-octadecenoic acid (as. oleat).
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:34:33
https://journal.ugm.ac.id/TradMedJ/article/view/8055
Majalah Obat Tradisional; Vol 16, No 3 (2011)
eng
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8062
2015-12-07T07:45:03Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
ANTIANGIOGENIC ACTIVITY OF METHANOLIC EXTRACT FROM AKAR PASAK BUMI (Eurycoma longifolia, Jack) ON CHORIOALLANTOIC MEMBRANE OF CHICKEN EMBRYO INDUCED BY bFGF
Salamah, Nina
Faculty of Pharmacy, University of Ahmad Dahlan
Sugiyanto, Sugiyanto
Faculty of Pharmacy, Universitas Gadjah Mada
Hartati, Mae Sri
Faculty of Medicine, Universitas Gadjah Mada
Hayati, Farida
Department of Pharmacy, Islamic University of Indonesia
angiogenesis; Eurycoma longifolia; pasak bumi; CAM; bFGF
Angiogenesis is a neovascularization process which its function is to supply nutrient and oxygen for cell survival. Pasak bumi is one of the Indonesian original plants having anti cancer activity. Extract of methanol, buthanol, kloroform, and water akar pasak bumi was proved to have cytotoxic effect on cancer cell culture. This research aim to know activity of antiangiogenic extract of metanol akar pasak bumi at chorioallantoic membrane of chicken embryo. Antiangiogenic activity test conducted by divide 8-9 days old egg embrio into 8 groups. Group I as paper disc control, group II as bFGF control, group III as bFGF control + DMSO 0.8% solvent, group IV, V, VI, VII, and VIII as groups of treatment. Each groups of treatment were gave by bFGF 1ng/µL extract of metanol akar pasak bumi respectively with concentration 20, 40, 60, 80 and 100 µg/mL concentration. After 3 days incubation, then the chorioallantoic membrane macroscopic and microcospically analized. The antiangiogenic activity extract of metanol akar pasak bumi at chorioallantoic membrane bFGF induce at used concentration, that is start from 40 µg/mL concentration. The increases of the concentration also increase the prohibition activity to angiogenesis.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-01-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8062
Majalah Obat Tradisional; Vol 15, No 1 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8063
2015-12-07T07:45:03Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
POTENCY OF CITRUS PEELS (Citrus aurantiifolia (Cristm.) Swingle) ETHANOLIC EXTRACT AS CHEMOPREVENTIVE AGENT THROUGH DOWNREGULATION OF c-Myc EXPRESSION AND INHIBITION OF 7.12-DIMETHYLBENZ[a]ANTRACHENE INDUCED FEMALE SPRAGUE DAWLEY RATS BREAST CELL PROLIFERATION
Pratiwi, Dewi
Faculty of Pharmacy, Universitas Gadjah Mada
Hastuti, Novi
Faculty of Pharmacy, Universitas Gadjah Mada
Nur W, Niken
Faculty of Pharmacy, Universitas Gadjah Mada
Armandari, Inna
Faculty of Pharmacy, Universitas Gadjah Mada
Ikawati, Muthi’
Faculty of Pharmacy, Universitas Gadjah Mada
Hermawan, Adam
Faculty of Pharmacy, Universitas Gadjah Mada
Meiyanto, Edy
Faculty of Pharmacy, Universitas Gadjah Mada
proliferation; Citrus aurantiifolia; AgNOR; Immunohistochemistry; c-myc
The using of natural-based medicine is growing rapidly in societies. Besides being cheap and affordable, natural-based medicine is relatively safer than the synthetic drugs. Peel of Citrus aurantifolia (Cristm.) Swingle) is one of the chemopreventive agent which contain flavonoids have potency as anticarcinogenic agent. This study is designed to study the potency of Citrus aurantifolia peel ethanolic extract in proliferation inhibition of Rattus norvegicus mammary cell of Sprague Dawley strain which is induced by 7,12-Dimethylbenz[a]anthracene (DMBA). Rats were divided into five groups consist of DMBA treatment, CMC-Na treatment, extract 1500 mg/kgBW treatment, treatment of DMBA+ extract 750 mg/kgBW and DMBA+ extract 1500 mg/kgBW. At the beginning of the tenth week of the study, breasts was isolated and stored in 10% formalin buffer. Observation of cell proliferation was done by AgNOR method. C-Myc expression observed using immunohistochemistry (IHC). Observation of mammary cell with AgNOR method indicated that the treatment of Citrus aurantifolia peel ethanolic extract can inhibit cell proliferation significantly. Dosage 1500 mg/kgBW gave higher inhibition effect than dosage 750 mg/kgBW. IHC result showed that treatment of Citrus aurantifolia peel ethanolic extract decrease the expression of c-Myc. Dosage 750 mg/kgBW gave lower decreasing effect than dosage 1500 mg/kgBW. Citrus aurantifolia peel ethanolic extract inhibited the proliferation of mammary cell induced DMBA through the inhibition of c-Myc expression in dose dependent phenomena so that it is a potential chemopreventive agent.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-01-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8063
Majalah Obat Tradisional; Vol 15, No 1 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8064
2015-12-07T07:45:03Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
THE EFFECT OF PINEAPPLE STEM ETHANOL EXTRACT OF (Ananas comosus (L.) TO APOPTOSIS OF ORAL TONGUE SGUAMOUS CELL CARCINOMA CELL LINE
Naritasari, Fimma
Faculty of Dentistry, Universitas Gadjah Mada
Susanto, Hendri
Faculty of Dentistry, Universitas Gadjah Mada
Supriatno, Supriatno
Faculty of Dentistry, Universitas Gadjah Mada
oral tongue squamous cell carcinoma; pineapple stem ethanol extract; apoptosis
Squamous cell carcinoma is one of the most common malignancy in the oral cavity. Currently, cancer treatment using surgery, radiotherapy, chemotherapy and its combination. New strategy for cancer therapy is by searching herbal which can induce apoptosis. Bromelain is one of a potential anticancer agent that can induce apoptosis. Pineapple stem is one of a potential herbal medicine because of its bromelain enzyme content. The aim of this study was to investigate the relation between pineapple stem ethanol extract and apoptosis of oral tongue squamous cell carcinoma cell line. Oral tongue squamous cell carcinoma cell line was treated with three concentrations under IC50 of pineapple stem ethanol extract, including 5.000, 5.500, 6.000 µg/ml. In apoptosis test, cell stained with fluorochrome ethidium bromide and acridine orange after 24 hours incubation. Fluorescence microscope was used for counting the cell. Viable cell would be stained green and apoptotic cell would be stained yellow to orange. Probit analysis was used to determine IC50 of pineapple stem ethanol extract on Oral tongue squamous cell carcinoma cell line. Pearson analysis used to know the correlation between pineapple stem ethanol extract concentration and Oral tongue squamous cell carcinoma cell line apoptosis. The result showed that there was a positive correlation between concentration of pineapple stem ethanol extract and apoptotic cell (r=0,999, p<0,05). In conclusion, pineapple stem ethanol extract treatment induced apoptosis of oral tongue squamous cell carcinoma and the increases of pineapple stem ethanol extract concentration followed by the increase of apoptosis induction.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-01-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8064
Majalah Obat Tradisional; Vol 15, No 1 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8065
2015-12-07T07:45:03Z
TradMedJ:ART
"100101 2010 eng "
2406-9086
1410-5918
dc
EFFECTS OF DRYING METHODS IN GAINING OF EXTRACTIVE, PHENOLIC CONTENT AND ANTIOXIDANT ACTIVITY IN Gynura pseudochina (Lour.)
Rivai, Harrizul
Faculty of Pharmacy, University of Andalas
Nurdin, Hazli
Faculty of Mathematics and Science, University of Andalas
Suyani, Hamzar
Faculty of Mathematics and Science, University of Andalas
Bakhtiar, Amri
Faculty of Pharmacy, University of Andalas
Antioxidant; Gynura pseudochina (L.) DC; drying methods; phenolic compounds
Effects of drying methods in gaining of extractive, phenolic content and antioxidant activity in Gynura pseudochina (Lour.) DC leaves have been investigated. The drying methods tested were air-drying at ± 25 oC, oven-drying at 40 OC, oven-drying at 60 OC, microwave oven-drying and fresh samples as control. Results revealed that drying of the fresh plant caused the decrease of extractive obtainability, phenolic content and antioxidant activity. There were significant differences among drying methods (P < 0.05). Among the drying methods tested, the highest extractive obtainability was by microwave oven-drying, whereas the highest phenolic concentration and antioxidant activity were by air-drying at ± 25 oC.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-01-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8065
Majalah Obat Tradisional; Vol 15, No 1 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8066
2015-12-07T07:45:03Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
EFFECTS OF FLAVONOIDS ISOLATED FROM ORANGE JASMINE (Murraya paniculata [L.] Jack.) ON HISTAMINE RELEASE FROM MAST CELLS
Nugroho, Agung Endro
Faculty of Pharmacy, Universitas Gadjah Mada
Riyanto, Sugeng
Faculty of Pharmacy, Universitas Gadjah Mada
Sukari, Mohamad Aspollah
Faculty of Science, Universiti Putra Malaysia
Maeyama, Kazutaka
Department of Pharmacology, Informational Biomedicine, Ehime University
flavonoid; Murraya paniculata; histamine; mast cells
Murraya paniculata [L.] Jack. (Kemuning) is a plant that grows widely in some areas of Indonesia. Studies related to this plant have been widely explored especially isolation of its active compounds. The plant contents several active compounds such as flanovoids. In the study, three flavonoid isolated from M. paniculata were evaluated for their effect on histamine release from mast cells (RBL-2H3 cells). These compounds were 3,3’,4’,5,5’,6,7,8-octamethoxyflavone; 3,3’,4’,5,5’,6,7-heptamethoxyflavone and 3, 3’, 4’, 5, 5’, 7–hexamethoxyflavone. The histamine inducers used in the study were DNP24-BSA dan thapsigargin, inducing the histamine release immunologically and non-immunologically, respectively. In the study, heptamethoxyflavone and hexamethoxyflavone did not influence the histamine release from mast cells significantly. However, octamethoxyflavone could increase the histamine release from RBL-2H3 cells in absence and presence the histamine inducers. The flavanoid could increase the release of histamine up to 50 %. Based on the results, polymethoxy moieties at the structure of flavonoid have a significant role to emerge the histamine-release stimulating effect from mast cells.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-01-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8066
Majalah Obat Tradisional; Vol 15, No 1 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8067
2015-12-07T07:45:03Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
ISOLATION AND IDENTIFICATION OF ANTIBACTERIAL COMPOUND FROM Eupatorium odoratum L. LEAVES AND ITS ACTIVITY AGAINST Staphylococcus aureus ATCC 25923 AND Escherichia coli ATCC 25922
Hasnawati, Hasnawati
Faculty of Mathematics and Science, University of Haluoleo
Prawita, Erna
Faculty of Pharmacy, Universitas Gadjah Mada
Eupatorium odoratum L.; chalcone; antibacterial compound; E. coli
The leaves of Eupatorium odoratum L. have been used traditionally to cure abscess due to infectious diseases, leech bite and wound healing. It was suspected that the leaves contain antibacterial compounds. The dried powder of E. odoratum leaves is extracted by Soxhlet using washbenzene and followed by methanol. Both extracts are tested to determine their antibacterial activity by using diffusion test. The active extract is fractionated by vacuum liquid chromatography. The active compound (2.3) obtained from the active fraction is purified by using preparative thin layer chromatography. The UV-Vis spectrum of (2.3) (chloroform) showed λmax at 291nm. The IR spectrum of the active compound (2.3) (KBr) .0displayed signals (-OH), (-CHalifatik), (-CHalifatik), (-C=O), (C=C), (CH2), (CH3), (epoksi), and (C-O). 1H-NMR spectrum (CDCl3) showed signals at δ 1.26 ( 3H,s); 3.65 (3H,s); 3.82 (3H,s); 3.93 (3H,s); 4.53 (1H,d); 5.0 (1H,d); 5.7 (1H,d); 6.06 (1H,d); 6.14 (1H,d); 6.93 (1H,d); 7.05 (1H,dd); dan 7.16 (1H,d) ppm. This spectrum is suspected to be an epoxide chalcone type of compound. The active compound (2.3) at minimum loading of 250 µg showed bacterial growth inhibition on S. aureus and E. coli at diameter 9.5 and 7.2 mm respectively.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-01-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8067
Majalah Obat Tradisional; Vol 15, No 1 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8069
2015-12-07T08:02:10Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
CYTOTOXIC ACTIVITY OF FRACTION n-HEKSANA : CHLOROFORM FROM METHANOLIC EXTRACT OF Rhizopora mucronata STEM BARKS ON MYELOMA CELL-LINES
Harwoko, Harwoko
Department of Pharmacy, University of Jenderal Soedirman
Utami, Esti Dyah
Department of Pharmacy, University of Jenderal Soedirman
Rhizopora mucronata; cytotoxic; myeloma;
The Mangrove plants have been long used for traditional medicine to cure gastroenteritis and cancer theraphy. Rhizopora mucronata, one species of Mangrove, is still rare studied that have potention as anti cancer. This research goals was to fractination bioactive compound of R. mucronata with various organic solvent and cytotoxicity test of those fraction n-heksana : chloroform to cancer cell myeloma. The extract from R. mucronata stem barks was made by maseration with methanol. The extracts was partitioned with chloroform, etil acetate, and methanol. The fraction of chloroform was fractination with n-heksana : chloroform (3 : 2). The fraction respectively was examined their cytotoxicity on Myeloma cell-line by MTT methods. Result showed that fraction n-heksana : chloroform from methanolic extract of R. mucronata stem barks was toxic on Myeloma cell, with the IC50 value was 15 µg/mLand phytochemical study showed that the active fractions contained flavonoid and terpenoid.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-05-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8069
Majalah Obat Tradisional; Vol 15, No 2 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8070
2015-12-07T08:02:10Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
FORMULATION CREAM CONTAINING ESSENTIAL OIL OF Curcuma heyneana : PHYSICAL CHARACTERISTICS TEST AND IN VITRO ANTIFUNGAL ACTIVITY AGAINST Candida albicans
Rahmawati, Dewi
Faculty of Pharmacy, University of Muhammadiyah Surakarta
Sukmawati, Anita
Faculty of Pharmacy, University of Muhammadiyah Surakarta
Indrayudha, Peni
Faculty of Pharmacy, University of Muhammadiyah Surakarta
essential oil; Curcuma heyneana; cream; O/W; W/A
Essential oil of Curcuma heyneana Val & Zijp has antifungal activity against Candida albicans with minimum inhibitory concentration (MIC) 0.25% v/v. Characteristic of essential oil is easy to evaporate from the skin surface. Therefore, it should be formulated in cream. The research aims to know influence of cream formulations of Curcuma heyneana oil toward physical characteristics and antifungal activity against Candida albicans. Essential oil was obtained by water and steam destillation. Cream were made into oil in water base (O/W) and water in oil base (W/O) containing 10% of Curcuma heyneana oil. Cream were tested for physical characteristics (such as viscosity, spreadability, protection ability, adhesive time, pH) and also in vitro antifungal activity against Candida albicans. Observation for antifungal activity was done after incubation for 48 hours. Data for physical characteristics and antifungal activity were analyzed using one way anava and continued with t-LSD test (p=0,95). The research indicated that W/O cream containing Curcuma heyneana oil had higher viscosity than O/W cream. Spreadability and protection ability O/W cream was better than W/O cream. Adhesive time of W/O cream was longer than O/W cream. Both of O/W and W/O cream had pH level of 6-7. O/W cream containing Curcuma heyneana oil had better inhibition zone against Candida albicans than cream W/O, they were 1.84±0.071 cm and 1.70±0.074 cm, respectively.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-05-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8070
Majalah Obat Tradisional; Vol 15, No 2 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8071
2015-12-07T08:02:10Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
TEST OF ANTIOXIDANT ACTIVITY AND DETERMINATION OF TOTAL ANTHOCYANIN CONTENT IN RIND OF MANGOSTEEN (Garcinia mangostana L)
Supiyanti, Wiwin
Sekolah Tinggi Ilmu Farmasi Yayasan Pharmasi
Wulansari, Endang Dwi
Sekolah Tinggi Ilmu Farmasi Yayasan Pharmasi
Kusmita, Lia
Sekolah Tinggi Ilmu Farmasi Yayasan Pharmasi
anthocyanin; rind of mangosteen; Garcinia mangostana L.; antioxidants
Mangosteen fruit with latin name Garcinia mangostana L. got the nickname Queen of tropical fruit (Tropical Fruits queen). Most of the mangosteen fruit is consumed in fresh condition. Mangosteen rind purple black. Mangosteen rind allegedly containing anthocyanin compounds. This study aimed to determine antioxidant activity of bark extracts of the mangosteen fruit and the total anthocyanin content in the rind of the mangosteen fruit. Object of research is that the mangosteen rind has been good to consume. Mangosteen rind mashed and then soaked with methanol containing 1% HCl. Antioxidant activity test mangosteen rind extract includes a qualitative test of antioxidant activity of extracts of TLC plates sprayed mangosteen rind 0.1 mM DPPH reagent produces patches of pale yellow with a purple background. Quantitative test of antioxidant activity based on the parameters EC50 using DPPH method with the comparison of vitamin C. Obtained EC50 value of 8.5539 tg/mL and EC50 values of vitamin C is 3.3676 tg/mL. Average total anthocyanin content in the skin of the mangosteen fruit is 59.3 mg in 100 grams of the mangosteen rind.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-05-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8071
Majalah Obat Tradisional; Vol 15, No 2 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8072
2015-12-07T08:02:10Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
ASCORBIC ACID CONTENT ON Hibiscus sabdariffa L. CALLUS AT VARIOUS CONSENTRATION OF SUCROSE IN MS MEDIA
Nurchayati, Yulita
Facullty of Mathematics and Science, University of Diponegoro
Afiah R, Fathiyah
Facullty of Mathematics and Science, University of Diponegoro
rosella callus; ascorbic acid; sucrose
Ascorbic acid is an antioxidant which is found in rosella flower. Its production through conventional method needs long time and wide field, so an alternative method through development of callus invitro is conducted. Sucrose is a main carbon source in MS medium and this sugar plays role as precursor for the formation of ascorbic acid. This study was conducted to determine the effect of sucrose concentration on callus formation and production of ascorbic acid. Sucrose was added to the media at the concentration of 20 g/L, 30 g/L, 40 g/L and 50 g/L. The culture was incubated for 42 days with twice subculture every 10 days. Analysis of ascorbic acid was perfomed using iodometry. The results showed that the callus was grow better in sucrose of 20 g/L while the ascorbic acid production was better in sucrose 50 g/L.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-05-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8072
Majalah Obat Tradisional; Vol 15, No 2 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8073
2015-12-07T08:02:10Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
LOZENGE FORMULATION OF GAMBIER (Uncaria gambir (Hunter) Roxb.) EXTRACT WITH VARIOUS CONCENTRATION OF GUMMI ACACIAE
Chabib, Lutfi
Department of Pharmacy, Islamic University of Indonesia
Triastuti, Asih
Department of Pharmacy, Islamic University of Indonesia
Irianti, Rischi Dwi
Department of Pharmacy, Islamic University of Indonesia
Gambier; Uncaria gambir (Hunter) Roxb.; gummi acaciae; direct compresion; lozenges
Gambier is one of the medicinal plants commonly used for chewing, which has benefits for oral health and dental. Gambier has a property as a remedy lozenges, stomachache, toothache, and can be used to prevent the formation of dental plaque. This research aims to create a dosage of dried extract in the form of lozenges. In these lozenges we used variations gummi acaciae to obtain a binder concentration that can produce tablets that meet the requirements. Gambier extract was obtained by remaceration, with 80% ethanol. Tablets formulated with a binder concentration gom arabic 10%, 17.5% and 25% using direct compression method. The results showed that the concentration variation can minimize the variation of the gummi acaciae weight uniformity, increased hardness, lower % fragility, and increase the time dissolved lozenges. All of tablets have good physical tablets properties. The third formula is the most acceptable by the respondents in terms of color, taste, and time of dissolution.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-05-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8073
Majalah Obat Tradisional; Vol 15, No 2 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8074
2015-12-07T08:02:10Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
Effect of Ethanolic Extract of "Jahe Merah" Rhizome in Macrophage Phagocytosis on Mice Infected by Listeria monocytogenes
Bintari, YS
Faculty of Pharmacy, Universitas Gadjah Mada
Sudarsono, Sudarsono
Faculty of Pharmacy, Universitas Gadjah Mada
Yuswanto, Agustinus
Faculty of Pharmacy, Universitas Gadjah Mada
jahe merah; macrophage; phagocytosis; mice; Listeria monocytogenes
Ginger is one of some medicinal plants that has been used to make someone still in the comfortable condition e.g. headache, diarrhea, flatulent, cold, cough, and masuk angin. The purpose of this study was to determine effects of pungent principle extract of the “jahe merah” rhizome in the relation of increasing macrophage phagocytic activation. This method was done by using latex beads.( Leijh et al., (1986) The effects of 10 mg/kgBW, 25 mg/kgBW, and 100 mg/kgBW of pungent principle extract of “jahe merah” rhizome ethanolic extract, that extracted by 70%v/v Ethanol was decided according to the number of latex which was phagocyted by macrophages. The conditioning time was done in 20 days. At 15th day, mice were infected by Listeria monocytogenes. The data was analyzed by Kolmogorov-Smirnov, and one way ANOVA with by 95% confidence level. As the specific parameter of the pungent principle extract were: pungent principle spot 1 : 2 = 6.8:1; 3.27%w/w GAE (Gallic Acid Equivalent) as total phenolic concentration, and IC50 14.57 mg/mL. The result was shown that 100 mg/kgBW of 70% ethanolic extract of “jahe merah” rhizome, was capable to increase macrophage activity. The effect of increasing macrophage phagocytosis of 100 mg/kgBW of 70% ethanolic extract of jahe merah was the same with Echinacea extract and Levamisol.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-05-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8074
Majalah Obat Tradisional; Vol 15, No 2 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8075
2015-12-14T09:37:32Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
FORMULA OPTIMATION OF SENGGUGU ROOT BARK EXTRACT LOZENGES (Clerodendrum serratum (L.) Moon.) AS MUCOLYTIC AGENT WITH LACTOSE – SORBITOL FILLER COMBINATION
Wahyono, Wahyono
Faculty of Pharmacy, Universitas Gadjah Mada
Kurniati, Luthfia Hanifah
Faculty of Pharmacy, Universitas Gadjah Mada
Sulaiman, TN Saifullah
Faculty of Pharmacy, Universitas Gadjah Mada
senggugu, lactose, sorbitol, simplex lattice design, lozenges
Kulit akar senggugu memiliki khasiat sebagai pengencer lendir (mukolitik) dan sudah digunakan secara empiris.Perlu dibuat sediaan tablet hisap agar dapat digunakan dengan nyaman dan praktis. Simplisia bentuk serbuk diekstraksi dengan maserasi menggunakan pelarut etanol 70%. Tablet hisap ekstrak kulit akar senggugu dioptimasi dengan menggunakan campuran bahan pengisi laktosa-sorbitol, yaitu formula A (100% laktosa), formula B (100% sorbitol), dan formula C (50% laktosa : 50% sorbitol). Tablet dibuat dengan metode granulasi basah. Formula optimum didapatkan dari hasil uji sifat fisik granul dan tablet hisap menggunakan metode SLD, lalu diuji sifat fisik meliputi sifat alir granul, kekerasan, waktu larut, tanggapan rasa serta dilakukan analisa kuantitatif kadar zat aktif dalam tablet hisap. Dari sifat fisik tablet hisap formula prediksi (SLD) dan verifikasi (optimum) diuji statistik menggunakan t-Test. Hasil penelitian menunjukkan bahwa kombinasi bahan pengisi laktosa-sorbitol dapat mempengaruhi sifat fisik granul dan tablet hisap kulit akar senggugu.Interaksi bahan pengisi laktosa-sorbitol dalam campuran dapat menaikkan respon kekerasan dan tanggap rasa tablet hisap, menurunkan respon kecepatan alir granul dan waktu larut tablet hisap. Formula optimum yang diperoleh dari campuran laktosa-sorbitol adalah 100% sorbitol : 0% laktosa.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8075
Majalah Obat Tradisional; Vol 20, No 2 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8076
2015-12-14T09:37:32Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
COMPARISON OF METHODS OF PRODUCING BEE PROPOLIS PURIFIED EXTRACT BASED ON TOTAL FLAVONOID CONTENT USING RUTIN AS STANDARD
Pramono, Suwijiyo
Faculty of Pharmacy, Universitas Gadjah Mada
Puspitasari, Agustina Dian
Faculty of Pharmacy, Universitas Gadjah Mada
propolis, flavonoid, purified extract, Zou method
Propolis adalah campuran kompleks lilin lebah, gula, dan getah pepohonan kumpulan lebah madu (Apis mellifera). Salah satu kandungan yang memiliki efek farmakologis adalah flavonoidnya. Flavonoid dapat diperoleh dalam kadar dan kemurnian tinggi dengan dibuat ekstrak terpurifikasi. Ekstraksi dilakukan dengan etanol 96%, dilanjutkan purifikasi dengan etanol 30%, kloroform dan etil asetat untuk metode I, serta air panas dan etil asetat untuk metode II. Kandungan flavonoid dideteksi dengan Kromatografi Lapis Tipis, fase diam silika gel 60F 254, fase gerak n-butanol-asam asetat-air (3:1:1 % v/v). Kadar flavonoid total diukur dengan spektrofotometer UV-Vis berdasarkan metode Zou, dihitung sebagai rutin. Hasil dianalisis dengan paired sample t-test. Rerata kadar flavonoid total dari ekstrak propolis belum terpurifikasi yaitu 1,23 ± 0,37 %b/b. Metode I dan II dapat digunakan untuk mempurifikasi ekstrak etanol 96% propolis, menghasilkan kadar flavonoid total yaitu 9,97 ± 1,57 (8,1 kali) dan 11,78 ± 1,30 %b/b (9,6 kali). Uji paired sample t-test dengan SPSS 16.00 menunjukkan efektivitas purifikasi dengan nilai Sig = 0,048.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8076
Majalah Obat Tradisional; Vol 20, No 2 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8077
2015-12-14T09:37:32Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
OPTIMIZATION OF LOZENGES FORMULA OF SENGGUGU ROOT BARK (Clerodendrum serratum L. Moon) EXTRACTS FOR MUCUS DILUENT (MUCOLITIC) IN COMBINATION WITH MANNITOL-LACTOSE-SUCROSE FILLERS
Sulaiman, T.N. Saifullah
Faculty of Pharmacy, Universitas Gadjah Mada
Puspita, Desi Elvira Cindy
Faculty of Pharmacy, Universitas Gadjah Mada
Wahyono, Wahyono
Faculty of Pharmacy, Universitas Gadjah Mada
senggugu root bark, sucrose, mannitol, lactose, optimization, lozenges, Simplex Lattice Design, Design Expert 7.1.5
Senggugu root bark (Clerodendrum serratum L. Moon) is known as a mucolitics. Senggugu root bark is made in the dosage form of lozenges in combination with sucrose, mannitol and lactose in order to obtain good flavor and comfortable when consumed. The purpose of this study was to determine the effect of the combination of fillers on the physical properties of granules and tablets as well as the composition of the combination of excipients to produce lozenges of extract of senggugu root bark with optimum physical properties. Seven formulas were made with a combination of mannitol, lactose and sucrose as follows: FI (100%: 0%: 0%), FII (0%: 100%: 0%), FIII (0%: 0%: 100%), FIV (50 %: 50%: 0%), FV (50%: 0%: 50%), FVI (0%: 50%: 50%), and FVII (33.3%: 33.3%: 33.3% ). The results showed that the combination of mannitol, lactose and sucrose affected the physical properties of the granules and lozenges of extract of senggugu root bark i.e. flow rate, hardness, dissolved time, compactibility and perceptive sense. The dominant amount of sucrose and lactose can improve the physical properties of granules and tablets. The optimal composition of the combination of mannitol, lactose and sucrose obtained from Design Expert 7.1.5. program was 5.491%: 37.387%: 57.122%, respectively.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8077
Majalah Obat Tradisional; Vol 20, No 2 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8078
2015-12-14T09:37:32Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
REPELLENT ACTIVITY TEST OF ESSENTIAL OIL OF BASIL LEAVES (Ocimum basilicum (L.) f. Citratum Back) AGAINST Aedes aegypti LOTION AND PHYSICAL CHARACTERISTICS TESTS OF THE LOTION
Murrukmihadi, Mimiek
Faculty of Pharmacy, Universitas Gadjah Mada
Fajarini, Dias Anita
Faculty of Pharmacy, Universitas Gadjah Mada
Aedes aegypti, essential oils, repellent, lotion, Ocimum basilicum
Minyak atsiri daun kemangi memiliki aroma khas berfungsi sebagai repelan sehingga dapat digunakan sebagai anti nyamuk. Lotion dianggap lebih praktis, mudah aplikasinya, mudah dioleskan, mudah dituang, dan lebih efisien sehingga repelan dibuat dalam bentuk lotion. Tujuan penelitian ini adalah mengetahui aktivitas repelan minyak atsiri daun kemangi (Ocimum basilicum (L.) f. Citratum Back) terhadap nyamuk Aedes aegypti dalam sediaan lotion. Daun kemangi dikeringanginkan, kemudian disuling dengan distilasi air dan uap selama ±6 jam untuk memperoleh minyak atsiri. Minyak atsiri dengan konsentrasi 15%v/v, 25%v/v, dan 35%v/v dalam etanol 95% dibuat dalam sediaan lotion. Sediaan lotion diuji sifat fisik (homogenitas, viskositas, daya sebar, dan daya lekat) dan uji aktivitas repelan. Data yang didapat dianalisis menggunakan analisis Kolmogorov Smirnov test, Anava 2 jalan, uji Turkey HSD test. Uji aktivitas repelan dianalisis dengan Anava 1 jalan dilanjutkan uji Turkey dengan taraf kepercayaan 95%.Hasil penelitian menunjukkan bahwa dengan kenaikan konsentrasi (15%v/v, 25%v/v, 35%v/v dalam etanol 95%),lotion yang dihasilkan homogen dan mudah dituang. Lotion dengan kenaikan konsentrasi minyak atsiri daun kemangi tersebut mempengaruhi aktivitas repelan yang berbeda bermakna dengan waktu perlindungan berturut-turut adalah 3522, 4611, dan 5435 detik.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8078
Majalah Obat Tradisional; Vol 20, No 2 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8079
2015-12-14T09:37:32Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
CHEWABLE LOZENGES OF LEGUNDI LEAF EXTRACT (Vitex trifolia L. ) WITH VARIATIONS IN THE PROPORTION OF BASE GLYCERINE-GELATIN
Sulaiman, TN. Saifullah
Faculty of Pharmacy, Universitas Gadjah Mada
Aryani, Dina
Faculty of Pharmacy, Universitas Gadjah Mada
Murti, Yosi Bayu
Faculty of Pharmacy, Universitas Gadjah Mada
-
Daun Legundi (Vitex trifolia L.) sering digunakan dalam ramuan obat tradisional di Indonesia sebagai antiasma. Penggunaan tanaman ini dalam pengobatan dirasa kurang praktis dan kurang dapat diterima, sehingga perlu dibuat dalam sediaan yang lebih sesuai, misalnya chewable lozenges. Bahan yang dapat memberi tekstur kenyal adalah gliserin dan gelatin sebagai basisnya. Tujuan penelitian ini adalah untuk mengetahui pengaruh variasi proporsi basis (gliserin dan gelatin) terhadap sifat fisik chewable lozenges dan ketersediaan kembali viteksikarpin dari sediaan. Chewable lozenges diproduksi dengan metode cetak tuang (molding mixture method). Formula yang dibuat adalah tiga formula dengan variasi proporsi gliserin dan gelatin yaitu Formula I (10 % : 90 %), Formula II (20 % : 80 %) dan Formula III (30 % : 70 %). Chewable lozenges yang terbentuk diuji sifat fisik meliputi : uji keseragaman bobot, waktu melarut, kadar air, dan KLT-densitometri, serta uji tanggap rasa menggunakan kuesioner oleh responden. Data yang diperoleh dianalisis statistik dengan analisis varian satu jalan taraf signifikansi (α) = 0,05. Hasil penelitian menunjukkan bahwa adanya variasi proporsi gliserin dan gelatin mempengaruhi sifat fisik chewable lozenges ekstrak daun legundi, yaitu semakin besar proporsi gelatin memperlama waktu larut (signifikansi < 0,05), dan semakin besar proporsi gliserin menyebabkan kadar air chewable lozenges semakin tinggi (signifikansi < 0,05). Formulasi chewable lozenges berpengaruh pada ketersediaan kembali viteksikarpin dari sediaan, semakin besar proporsi gelatin maka kadar relatif viteksikarpin yang diperoleh kembali menjadi lebih rendah. Berdasarkan hasil uji tanggap rasa chewable lozenges ekstrak daun legundi secara subjektif, Formula II (20 % : 80 %) paling disukai oleh responden.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8079
Majalah Obat Tradisional; Vol 20, No 2 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8080
2015-12-14T09:37:32Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
THE EFFECT OF HARVESTING TIME AND DEGREE OF LEAVES MATURATION ON VITEKSIKARPIN LEVEL IN LEGUNDI LEAVES (Vitex trifolia L.)
Murti, Yosi Bayu
Faculty of Pharmacy, Universitas Gadjah Mada
Dwinatari, Indrawan Kurnia
Faculty of Pharmacy, Universitas Gadjah Mada
Legundi leaves, viteksikarpin, harvesting time, degree of maturation
Legundi (Vitex trifolia L.) merupakan salah satu tanaman tradisional Indonesia yang belum banyak diteliti dan belum dikembangkan menjadi fitofarmaka. Daun legundi dapat digunakan untuk terapi pada penderita asma dengan kadar optimum. Oleh karena itu perlu dilakukan optimasi pemanenan daun legundi yang meliputi waktu dan tingkat maturasi daun. Optimasi waktu pemanenan dengan cara memanen daun legundi pada waktu yang berbeda yaitu pagi, siang, dan sore hari, sedangkan untuk optimasi tingkat maturasi daun dengan cara memanen daun nomor satu sampai lima dari ujung termuda, selanjutnya ditetapkan waktu pemanenan dan daun yang memberikan kadar viteksikarpin optimum. Pengukuran kadar viteksikarpin ditetapkan secara KLT-densitometri kemudian dianalisis menggunakan software Wincats dan Microsoft Office Excel 2007. Kadar viteksikarpin dalam daun legundi paling tinggi dipanen pada sore hari, kemudian siang hari, dan paling rendah pada pagi hari. Kadar viteksikarpin dalam daun legundi paling tinggi pada daun ke-2, kemudian daun ke-1, daun ke-3, daun ke-4, dan paling rendah pada daun ke-5. Pemanenan yang memberikan kadar viteksikarpin optimum yaitu pemanenan di sore hari dengan memetik daun ke-1 sampai daun ke-3.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8080
Majalah Obat Tradisional; Vol 20, No 2 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8081
2015-12-14T09:37:32Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
ISOLATION AND DETERMINATION OF KEMBANG SEPATU FLOWERS (Hibiscus rosa-sinensis L.) ETHANOLIC EXTRACT USING SPECTRODENSITOMETRY
Murrukmihadi, Mimiek
Faculty of Pharmacy, Universitas Gadjah Mada
Pamungkas, Kurniati
Faculty of Pharmacy, Universitas Gadjah Mada
H. rosa-sinensis flowers, compound isolation, concentration determination, alkaloid, spectrodensitometry
Penelitian ini bertujuan untuk melakukan isolasi dan mengetahui kadar relatif alkaloid pada bunga kembang sepatu (Hibiscus rosa-sinensis L.) secara spektrodensitometri. Ekstrak etanolik bunga kembang sepatu dibuat dengan metode maserasi menggunakan pelarut etanol 70%. Pemisahan senyawa alkaloid dilakukan dengan fraksinasi menggunakan VLC (Vaccum Liquid Chromatography) dan menggunakan campuran pelarut metanol-etilasetat dengan variasi konsentrasi. Hasil isolasi selanjutnya digunakan sebagai kurva baku, kemudian ditetapkan kadar sampel ekstrak bunga kembang sepatu secara spektrodensitometri dengan menggunakan fase diam silika gel dan fase gerak etil asetat: metanol (1:5 v/v). Kadar relatif alkaloid dihitung menggunakan kurva baku Y = 29023,75 X + 5809,35, r = 0,996. Didapatkan kadar relatif alkaloid sebesar 0,210 ± 0,035 % dan dapat diijadikan sebagai suatu senyawa penanda.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8081
Majalah Obat Tradisional; Vol 20, No 2 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8082
2015-12-14T09:37:32Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
EFFERVESCENT TABLETS FORMULATION OF GINGER RHIZOME (Zingiber officinale Rosc.) WITH VARIATION OF CITRIC ACID AND TARTARIC ACID LEVEL
Mufrod, Mufrod
Faculty of Pharmacy, Universitas Gadjah Mada
Kartikasari, Sekararum Diah
Faculty of Pharmacy, Universitas Gadjah Mada
Murti, Yosi Bayu
Faculty of Pharmacy, Universitas Gadjah Mada
Zingiber officinale Rosc. (jahe), effervescent tablet, variation of acid source
Jahe (Zingiber officinale Rosc.) memiliki khasiat sebagai anti emetik. Rimpang jahe biasanya dikonsumsi sebagai minuman penyegar instan seduhan sehingga perlu dibuat menjadi sediaan yang lebih efektif, efisien dan menarik. Penelitian ini bertujuan untuk memformulasikan rimpang jahe menjadi sediaan tablet effervescent dengan menggunakan variasi kadar asam sitrat dan asam tartrat. Ekstrak kering jahe dibuat dengan metode ekstraksi menggunakan etanol 70% dan diuapkan dengan spray dryer. Ekstrak dibuat menjadi 5 formula dengan variasi jenis dan jumlah sumber asam menggunakan metode peleburan. Granul yang diperoleh diuji sifat fisiknya meliputi uji waktu alir, indeks tap, sudut diam, daya serap air, kompaktibilitas, densitas massa, kadar air dan kadar fenolik total. Granul tersebut ditablet dan diuji sifat fisik tablet meliputi keseragaman bobot, kerapuhan, kekerasan, waktu larut, uji tanggap rasa, dan kadar fenolik total. Data yang diperoleh dianalisis menggunakan Anova One Way dengan taraf kepercayaan 95%. Hasil uji sifat fisik granul dan tablet effervescent ekstrak rimpang jahe menunjukkan bahwa formula III (50% asam sitrat) merupakan formula terbaik karena memenuhi persyaratan fisik granul dan tablet. Sedangkan formula V (100% asam tartrat) merupakan formula yang memberikan kestabilan kadar fenolik yang paling besar.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 00:00:00
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8082
Majalah Obat Tradisional; Vol 20, No 2 (2015)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8083
2015-11-24T13:16:37Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
FLAVONOID PROFILE OF Clitoria ternatea Linn
Ponnusamy, Selvamaleeswaran
Research and Development centre, Bharathiar University, Coimbatore,Tamil Nadu, India
Gnanaraj, Wesely Ebenezer
Department of Botany, Arignar Anna Government Arts College, Namakkal,Tamil Nadu, India
Antonisamy, Johnson Marimuthu
Department of Botany, St. Xavier’s College (Autonomous), Palayamkottai, India
Clitoria ternatea; HPTLC; Chromatography; Flavonoids
The present study was aimed to reveal the flavonoid profile of Clitoria ternatea L leaves, stem and seeds using the HPTLC analysis. The powdered leaves, stem and seed samples were extracted with 150 mL of methanol for 8-12 h by using the Soxhlet apparatus. The Ethyl acetate-butanone-formic acid-water (5:3:1:1) was employed as mobile phase for flavonoids. The developed plate was sprayed with 1% ethanolic aluminium chloride as spray reagent and dried at 100°C in hot air oven for 2min. The plate was photo-documented at UV 366 nm and daylight using Photo-documentation chamber.The methanolic extract of stem, leaves and seeds of Clitoria ternatea showed the presence of 24 bands with 18 different Rf values with range 0.01 to 0.96. Out of 24 bands, 10 bands with seven Rf values viz., 0.04, 0.10, 0.28, 0.37, 0.49, 0.65 and 0.85 were idenified as flavonoids, the other bands were noted as unknown metabolites. Developed HPTLC chromatogram of Clitoria ternatea methanolic extracts of vegetative and reproductive parts could be used efficiently for identification, and quality assessment of the plant in the pharmaceutical industries. These profiles may used as chemical marker to solve plant systematic problems.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:16:37
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8083
Majalah Obat Tradisional; Vol 19, No 1 (2014)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8084
2015-11-24T13:16:37Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
RADICAL SCAVENGING ACTIVITY OF MILK BANANA (Musa paradisiaca L.) and AMBON BANANA (Musa paradisiaca L.) ON RADICAL 2,2-diphenyl-1-pikril hidrazil (DPPH)
Saputro, Anjar Hermadi
Faculty of Pharmacy, Universitas Gadjah Mada
Sudarsono, Sudarsono
Faculty of Pharmacy, Universitas Gadjah Mada
milk banana, ambon banana, an antioxidant, DPPH
Kandungan gizi termasuk vitamin A dan C pada pisang susu dan pisang ambon diprediksikan berpotensi sebagai antioksidan. Penelitian ini bertujuan untuk mengetahui potensi antiradikal antara buah pisang susu dan pisang ambon dengan vitamin C sintetik sebagai pembanding sehingga didapatkan seberapa besar kemampuan daya antioksidan pisang susu dan ambon yang dihasilkan dalam pengonsumsian buah bila diinginkan kekuatan antioksidan yang dihasilkan oleh vitamin C murni pada kadar tertentu. Penelitian ini terdiri atas pengukuran aktivitas antioksidan dengan metode 2,2-Difenil-1-Pikrilhidrazil (DPPH) sehingga didapatkan data aktivitas antioksidan dan Inhibitory Concentration50 (IC50)dari pisang susu, pisang ambon dan vitamin C sintetik. Dari hasil penelitian dapat disimpulkan bahwa sebanyak 2418,84g pisang susu berpotensi sebagai antioksidan yang setara dengan potensi antioksidan 1 gram vitamin C bila diukur dengan pereaksi DPPH 0,4mM, sedangkan sebanyak 9229,79g pisang ambon berpotensi sebagai antioksidan yang setara dengan potensi antioksidan vitamin C 1 gram bila diukur dengan pereaksi DPPH 0,4mM.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:16:37
https://journal.ugm.ac.id/TradMedJ/article/view/8084
Majalah Obat Tradisional; Vol 19, No 1 (2014)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8085
2015-11-24T13:16:37Z
TradMedJ:ART
"151124 2015 eng "
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1410-5918
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AN ANTIHYPERURICEMIA EFFECT OF ETHANOL EXTRACT OF TUNJUK LANGIT RHIZOME (HELMYNTHOSTACHYS ZAYLANICA LINN HOOK) ON SWISS MALE MICE
Fitrya, Fitrya
Department of Pharmacy, University of Sriwijaya
Muharni, Muharni
Department of Pharmacy, University of Sriwijaya
in vivo, Helminthostachys zaylanica, hipouricemia
Akar tumbuhan tunjuk langit (Helminthostachys zeylanica) sebagai ramuan obat tradisional diantaranya sebagai obat kanker dan mengobati radang. Beberapa penelitian membuktikan bahwa akar tumbuhan tunjuk langit menunjukkan aktivitas antioksidan, antikaker, neoroprotektif dan antiinflamasi. Penelitian ini bertujuan untuk melihat efek hipourikemia ekstrak etanol akar tunjuk langit terhadap kadar asam urat darah mencit yang diinduksi dengan jus hati ayam dibanding alopurinol. Penelitian ini menggunakan mencit putih jantan galur Swiss yang dibagi kedalam 10 kelompok (tiap kelompok 5 ekor) terdiri dari Kelompok I : Normal (CMC-Na 0,5% 10 mL/kg BB), Kelompok II : Kontrol negatif, Kelompok III-VI : Kontrol positif (alopurinol dengan dosis 5, 10, 20 dan 40 mg/kg BB) dan Kelompok VII-X : Ekstrak etanol dengan dosis 20, 40, 80, dan 160 mg/kg BB. Perlakuan terhadap hewan percobaan adalah selama 30 hari. Hewan uji diukur kadar asam urat serumnya pada hari ke-0 kemudian diberi jus hati ayam 25 ml/kg BB dua kali sehari ditambah melinjo (2g/kg BB perhari) dimulai dari hari ke-0 sampai hari ke 30. Pada hari ke-10 dan 15 kadar asam urat hewan uji diukur. Pada hari ke-15 sampai hari ke-30 dimulai pemberian sediaan uji per oral masing-masing dosis dengan tetap diberikan jus hati ayam. Kadar asam urat hewan uji diukur pada hari ke 20, 25 dan 30. Kemudian dihitung persentase penurunan kadar asam uratnya. Hasil uji secara in vivo menunjukkan ekstrak etanol akar tunjuk langit dosis 80 mg/kg BB memiliki potensi 91,95% dibanding alopurinol dosis 20 mg/kg BB dan ekstrak etanol dosis 160 mg/kg BB memiliki potensi 91.77% dibanding alopurinol dosis 40 mg/kgBB. Berdasarkan hubungan persen efek hipourikemia terhadap konsentrasi ekstrak aktif maka nilai ED50 ekstrak aktif adalah 135,76 mg/kg BB.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:16:37
https://journal.ugm.ac.id/TradMedJ/article/view/8085
Majalah Obat Tradisional; Vol 19, No 1 (2014)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8086
2015-11-24T13:16:37Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
HOMA-IR INDEX EVALUATION ON ANTIDIABETES MELLITUS EFFECT OF Andrographis paniculata (Burm. f.) Nees PURIFIED EXTRACT AND ANDROGRAPHOLIDE
Adriawan, Ignatius Ryan
Faculty of Medicine, Universitas Gadjah Mada
Andrie, Mohamad
Faculty of Medicine, Tanjungpura University
Susilowati, Rina
Faculty of Medicine, Universitas Gadjah Mada
Pramono, Suwidjiyo
Faculty of Pharmacy, Universitas Gadjah Mada
Nugroho, Agung Endro
Faculty of Pharmacy, Universitas Gadjah Mada
purified extract of Andrographis paniculata, Andrographolide, HOMA-IR index, High fat and fructose diet
Diabetes mellitus (DM) tipe 2 dipicu oleh diet tinggi lemak dan fruktosa (DTLF) dan diawali dengan resistensi insulin dan hiperinsulinemia kompensatori, yang dapat dilihat dari indeks homeostatic model assessment – insulin resistance (HOMA-IR). Salah satu tanaman tradisional yang dapat digunakan untuk mengatasi DM adalah Andrographis paniculata (Burm. f.) Nees, dengan senyawa aktif utama andrografolid. Penelitian ini bertujuan untuk mengetahui efek ekstrak terpurifikasi A. paniculata dan andrografolid pada indeks HOMA-IR tikus Wistar dengan DTLF. Penelitian bersifat kuasi-eksperimental dan analisis data menggunakan uji Kruskal-Wallis. Hasil menunjukkan bahwa ekstrak terpurifikasi A. paniculata dan andrografolid dapat menurunkan indeks HOMA-IR, dan penurunan terbesar (82,05%) dihasilkan oleh ekstrak terpurifikasi A. paniculata dengan dosis 1303,8 mg/kgBB.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:16:37
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8086
Majalah Obat Tradisional; Vol 19, No 1 (2014)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8087
2015-11-24T13:16:37Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
CYTOTOXIC ACTIVITY OF ETHANOLIC EXTRACT OF Persea americana Mill. LEAVES ON HeLa CERVICAL CANCER CELL
Mardiyaningsih, Ana
D3 Diploma of Pharmacy, Poltekkes Bhakti Setya
Ismiyati, Nur
D3 Diploma of Pharmacy, Poltekkes Bhakti Setya
Persea americana Mill., Cervical cancer, HeLa cell line, cytotoxic activity, thin layer chromatography
Kanker leher Rahim merupakan salah satu kanker yang paling terjadi pada wanita. Angka kematian yang tinggi menunjukkan bahwa kemoterapi tidak memberikan hasil yang baik sehingga perlu dikembangkan strategi untuk mengatasinya. Daun alpukat (Persea americana Mill) adalah salah satu produk alami yang memiliki aktivitas antioksidan dengan menangkap radikal bebas dan memiliki aktivitas sitotoksik. Tujuan dari penelitian ini adalah untuk mengevaluasi aktivitas sitotoksik dari ekstrak etanolik daun alpukat pada sel kanker leher Rahim HeLa dan mengidentifikasi senyawa aktif yang ada pada daun alpukat. Senyawa aktif dari daun alpukat diekstraksi dengan metode maserasi menggunakan etanol 96%. Uji kromatografi dilakukan untuk mengidentifikasi senyawa aktif. Aktivitas sitotosik ekstrak etanolik daun alpukat dilakukan dengan uji MTT. Hasil skrining fitokimia menunjukkan bahwa daun alpukat mengandung senyawa golongan flavonoid, alkaloid dan saponin. Ekstrak etanol daun alpukat menghambat pertumbuhan sel HeLa dengan nilai IC50 sebesar360 µg/ml.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:16:37
https://journal.ugm.ac.id/TradMedJ/article/view/8087
Majalah Obat Tradisional; Vol 19, No 1 (2014)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8088
2015-11-24T13:16:37Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
OPTIMATION OF GREEN TEA WASTE AXTRACTION USING MICROWAVE ASSISTED EXTRACTION TO YIELD GREEN TEA EXTRACT
Handayani, Dwi
Faculty of Pharmacy, University of Indonesia
Mun’im, Abdul
Faculty of Pharmacy, University of Indonesia
S. Ranti, Anna
Faculty of Pharmacy, University of Indonesia
antioxidants, extract green tea, green tea oil, green tea waste, MAE
Indonesia memiliki kekayaan keanekaragaman hayati yang besar, namun sayangnya potensi sumber daya alam yang tersedia masih sedikit yang diteliti dan digunakan dalam pengobatan. Salah satu tanaman yang banyak dibudidayakan di Indonesia adalah teh. Ampas teh adalah limbah dari industri teh, tersedia cukup banyak dan belum dimanfaatkan secara optimal. Penelitian ini bertujuan untuk mendapatkan kondisi ekstraksi ampas teh hijau menggunakan metode MAE (Microwave Assisted Extraction) yang paling efektif sehingga dihasilkan ekstrak teh hijau yang mempunyai antioksidan optimum. Dari hasil penelitian, kondisi optimum MAE adalah daya 450 W, pelarut etanol 60%, perbandingan simplisia – pelarut 1 : 8 dan lama ekstraksi 8 menit untuk menghasilkan ekstrak dengan kadar polifenol optimum. Ekstrak yang diperoleh mempunyai kadar air 7,83 %; pH 5,5; dan polifenol total, flavonoid total, asam galat, katekin, EGCG serta kofein berturut-turut sebesar 7,1%; 1,04%; 2,76%; 0,83%; 5,18% dan 1,03%. Dibandingkan dengan acuan yang ada, ekstrak yang diperoleh memenuhi syarat sebagai bahan baku kosmetik.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:16:37
https://journal.ugm.ac.id/TradMedJ/article/view/8088
Majalah Obat Tradisional; Vol 19, No 1 (2014)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8089
2015-11-24T13:16:37Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
ACTIVE CUTANEOUS ANAPHYLAXIS-INHIBITORY ACTIVITY OF THE N-HEXANE EXTRACT OF Morinda citrifolia L. FRUITS IN HEPATITIS B VACCINE-INDUCED WISTAR RATS
Sasmito, Ediati
Faculty of Pharmacy, Universitas Gadjah Mada
Nugroho, Agung Endro
Faculty of Pharmacy, Universitas Gadjah Mada
V. Sagala, Yose
Faculty of Pharmacy, Universitas Gadjah Mada
Morinda citrifolia L., inflammatory cells, active cutaneous anaphylaxis reaction
Mengkudu (Morinda citrifolia L.) adalah salah satu tanaman berkhasiat obat yang sudah tidak asing lagi penggunaannya dalam pengobatan. Mengkudu telah diketahui memiliki efek farmakologis, antara lain efek analgesik, antihipertensi, dan imunomodulator. Penelitian ini bertujuan untuk membuktikan bahwa ekstrak n-heksana buah mengkudu dapat menghambat reaksi anafilaksis kutaneus aktif pada tikus Wistar jantan yang diinduksi vaksin hepatitis B. Penelitian ini menggunakan metode anafilaksis kutaneus aktif, kemudian dilanjutkan dengan pengamatan histopatologinya. Uji anafilaksis kutaneus aktif dilakukan selama dua minggu. Hewan uji dibagi menjadi lima kelompok yaitu kelompok : kontrol tanpa perlakuan, perlakuan dengan ekstrak n-heksana buah mengkudu dosis 50mg, 100mg, dan 200mg/kgBB tikus, dan kelompok yang diberi kromolin dosis 2,16mg/kg BB tikus sebagai kontrol positif. Hewan uji disensitisasi 2 kali (1x setiap minggu) dengan vaksin hepatitis B (dosis 145µg/kgBB tikus) secara subkutan pada bagian punggung. Pada minggu kedua, hewan uji diambil secara acak 4 ekor setiap kelompok untuk dikurbankan dan diambil jaringan kulit beserta jaringan hatinya. Jaringan dibuat sebagai preparat histopatologi dengan pewarnaan hematoxyllin-eosin. Hasil penelitian menunjukkan bahwa pemberian ekstrak n-heksana buah mengkudu dosis 50mg, 100mg, dan 200mg/kgBB pada tikus jantan Wistar yang diinduksi vaksin hepatitis B dapat menghambat reaksi anafilaksis kutaneus aktif.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:16:37
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8089
Majalah Obat Tradisional; Vol 19, No 1 (2014)
ind
Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8090
2015-11-24T13:16:37Z
TradMedJ:ART
"151124 2015 eng "
2406-9086
1410-5918
dc
THE COMPARISON OF EXTRACTION METHOD AND SOLVENT VARIATION ON YIELD AND ANTIOXIDANT ACTIVITY OF Brassica oleracea L. var. capitata f. rubra EXTRACT
Senja, Rima Yulia
Faculty of Pharmacy, Universitas Gadjah Mada
Issusilaningtyas, Elisa
Faculty of Pharmacy, Universitas Gadjah Mada
Nugroho, Akhmad Kharis
Faculty of Pharmacy, Universitas Gadjah Mada
Setyowati, Erna Prawita
Faculty of Pharmacy, Universitas Gadjah Mada
Red cabbage extract, extraction method, solvent
Kubis ungu (Brassica oleracea L. var. capitata f. rubra) memiliki kandungan antosianin tinggi sehingga potensial sebagai antioksidan alami. Dalam penelitian ini, aktivitas antioksidan ekstrak kubis ungu dievaluasi secara kuantitatif dengan metode spekstroskopi menggunakan pereaksi DPPH (1,1-difenil-2-pikrilhidrazil) untuk mendapatkan nilai IC50. Penelitian ini dibagi menjadi dua tahap. Pertama, perbandingan variasi pelarut terhadap rendemen ekstrak serbuk kubis ungu dan panjang gelombang (λ) maksimum, menggunakan ekstraksi secara maserasi. Jenis pelarut yang digunakan (etanol 70%, 80%,95%, 96%) yang ditambahkan asam sitrat 3%. Kedua, perbandingan variasi metode ekstraksi dalam suasana netral terhadap aktivitas antioksidan secara kuantitatif. Hasil penelitian menunjukkan maserasi serbuk kubis ungu dengan pelarut etanol 96% (suasana asam) menghasilkan rendemen tertinggi. Soxhletasi kubis ungu segar dengan pelarut etanol 96% (suasana netral) memiliki (λ) maksimum 288,5 nm dan IC50 sebesar 168,78 µg/mL.
Faculty of Pharmacy, Universitas Gadjah Mada
2015-11-24 20:16:37
application/pdf
https://journal.ugm.ac.id/TradMedJ/article/view/8090
Majalah Obat Tradisional; Vol 19, No 1 (2014)
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Copyright (c) 2015 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8091
2016-12-23T07:27:25Z
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2016-12-23T07:27:25Z
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oai:jurnal.ugm.ac.id:article/8095
2020-07-24T06:30:31Z
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oai:jurnal.ugm.ac.id:article/8096
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oai:jurnal.ugm.ac.id:article/8097
2016-12-23T07:27:25Z
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TradMedJ:ART
oai:jurnal.ugm.ac.id:article/8131
2015-12-07T08:32:21Z
TradMedJ:ART
"151127 2015 eng "
2406-9086
1410-5918
dc
MUTAGENIC EFFECT OF ETHANOL EXTRACT OF Jatropha curcas L SEED SOLID WASTE OBTAINED FROM RESIDUAL FUEL VEGETABLE PROCESSING (BIOFUEL)
Wahyuningrum, Retno
Faculty of Pharmacy, University of Muhammadiyah Purwokerto
Wirasutisna, Komar Ruslan
School of Pharmacy, Bandung Institute of Technology
Elfahmi, Elfahmi
School of Pharmacy, Bandung Institute of Technology
Wibowo, Marlia Singgih
School of Pharmacy, Bandung Institute of Technology
Jatropha curcas; seedcake; mutagenicity effect
Jatropha curcas seed contains viscous oil that can be used for soap making, cosmetic and as biofuel. It contains phorbol ester that was toxic. Biofuel production of Jatropha curcas seed left seedcake from mechanical press process. For safety evaluation, mutagenicity test was carried out. The seedcake was extracted by maceration method at room temperature with methanol and the mutagenic effect was evaluated by Ames test against Salmonella typhimurium TA 1535 with or without S9 metabolic activator. Methanolic extract of Jatropha curcas seedcake had no mutagenicity effect against S. typhimurium TA 1535.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-09-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8131
Majalah Obat Tradisional; Vol 15, No 3 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8132
2015-12-07T08:29:25Z
TradMedJ:ART
"151127 2015 eng "
2406-9086
1410-5918
dc
ENHANCING ANTIFUNGAL CANDIDA ALBICANS ACTIVITY OF Piper bettle LINN. LEAF ESSENTIAL OIL OINTMENT THROUGH FORMATION OF COMPLEX WITH B-CYCLODEXTRIN INCLUSION
Astuti, Ika Yuni
Faculty of Pharmacy, University of Muhammadiyah Purwokerto
Hartanti, Dwi
Faculty of Pharmacy, University of Muhammadiyah Purwokerto
Aminiati, Ani
Faculty of Pharmacy, University of Muhammadiyah Purwokerto
Piper betle essential oil, inclusion complex, thin layer chromatography, powder X ray diffraction, antigfungal activity, candida albicans
The Piper betle leaf essential oil have antimicrobial activity to Candida albicans (Wirna, 2006). However, the essential oil is unstable to high temperature, light, and oxygen. One approach to improve the stability and facilitate in developing various dosage forms is by preparing the essential oil in complex inclusion using β-cyclodextrin (Szejtli, 1989). The aim of the present study was to investigate the effect of inclusion complex formation of the Piper betle leaf essential oil in b-cyclodextrin formulated to polyethylene glicol ointment to antifungal candida albicans activity. The essential oil of Piper betle leaf was isolated by destillation. The inclusion complex of the essential oil - β-cyclodextrin was prepared by coprecipitation of the essential oil : β- cyclodextrin 1:1 (w/w). The inclusion complex formation was studied with thin layer chromatography (TLC) and powder X-ray diffraction (XRD). In the TLC, sillica gel was used as solid phase, whereas toluen-ethyl acetate (7:3) and methanol-water (7:3) was used as mobile phase, then the Rf value of the essential oil and inclusion complex was compared. The XRD pattern of β-cyclodextrin, physical mixtures and inclusion complex was compared, too. Then, the polyethylene glicol ointment of the essential oil and inclusion complex was prepared. The physical properties and antifungal activity was compared. The TLC result showed that the essential oil of Piper betle leaf with methanol-water (7:3) as mobile phase had Rf 0,69 and the inclusion complex had Rf 0,81. With toluen-ethyl acetate (7:3), Rf of the essential oil were 0,63; 0,73; 0,85, whereas the inclusion complex were 0,66; 0,80, and 0,91. The powder XRD analysis showed that the pattern of β-cyclodextrin, physical mixture and inclusion complex were different. The differences of Rf from TLC and XRD patterns were showed that the Piper betle leaf essential oil was complexed with β cyclodextrin. The physical properties studies showed that the homogenity of both formulation was good. The inclusion complex ointment had spreading property, adhering property and antifungal activity which better than the essential oil ointment. The potency of antifungal activity of the inclusion complex of the essential oil - β-cyclodextrin was not significant different than miconazol ointment.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-09-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8132
Majalah Obat Tradisional; Vol 15, No 3 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8133
2015-12-07T08:32:21Z
TradMedJ:ART
"151127 2015 eng "
2406-9086
1410-5918
dc
AWAR–AWAR (Ficus septica Burm. f.) LEAVES ETHANOLIC EXTRACT INDUCED APOPTOSIS OF MCF-7 CELLS BY DOWNREGULATION OF Bcl-2
Sekti, Dewi Arum
Faculty of Pharmacy, Universitas Gadjah Mada
Mubarok, Muhammad Fithrul
Faculty of Pharmacy, Universitas Gadjah Mada
Armandani, Inna
Faculty of Pharmacy, Universitas Gadjah Mada
Junedy, Sendy
Faculty of Pharmacy, Universitas Gadjah Mada
Meiyanto, Edy
Faculty of Pharmacy, Universitas Gadjah Mada
Awar-awar (F. septica Burm. f.), MCF-7 cells, apoptosis, Bcl-2
Alkaloid fenantroindolisidin showed cytotoxic activity against several cancer cells. Ethanolic leaf extract of Awar-awar proven to increase the cytotoxic activity of chemotherapy agent doxorubicin in MCF-7 cells. This study aims to determine the effect of Ethanolic leaf extract of Awar-awar in apoptosis induction of breast cancer cells MCF-7.Awar-awar leaf powder was extracted by maceration using 70% ethanol and then concentrated to obtain concentrated of Awar-awar. Apoptosis induction was examined by double staining method and the expression of Bcl-2 protein was observed by immunohistochemistry method. Single treatment of Awar-awar leaf extract showed the ability of extracts to induce apoptosis and downregulated the expression of Bcl-2 protein. Ethanolic leaf extract of Awar-awar is potential as co-chemotherapeutic agent.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-09-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8133
Majalah Obat Tradisional; Vol 15, No 3 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8134
2015-12-07T08:29:25Z
TradMedJ:ART
"151127 2015 eng "
2406-9086
1410-5918
dc
FORMULATION OF ANTIOXIDANT LOTION CONTAINING WATER EXTRACT OF GREEN TEA LEAF (Camellia sinensis L)
Faramayuda, Fahrauk
Department of Pharmacy, University of Jenderal Ahmad Yani
Alatas, Fikri
Department of Pharmacy, University of Jenderal Ahmad Yani
Desmiaty, Yesi
Department of Pharmacy, University of Jenderal Ahmad Yani
extract, lotion, antioxidant
The research about lotion dosage formulation that contain green tea leaf water extract with concentration 0.0002%, 0.002%, 0,02%, and 8.6% had been done. The basic of the EC50 value. Which calculated from linierity regression equation between tested solution concentration and scavenging percent. The lotion formulation began with lotion base orientation included organoleptic, homogeinity, pH, and viscosity. It showed that lotion base with glyceryl monostearate 5.5% have the best pH stability, viscosity, and consistency. Evaluation of lotion dosage include physical evaluation (organoleptic, homogeinity, viscosity and stability) and chemical evalution (pH and the stability of lotion antioxidant activity. Antioxidant activity stability evaluation of lotion with 1,1-diphenyl-2-picrylhidrazyl solution using spectrophotometri UV-Visible showed, that formula which contain 0.02% and 8.6% green tea leaf water extract gave the best radical scavenging activity and stable during storage.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-09-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8134
Majalah Obat Tradisional; Vol 15, No 3 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8135
2015-12-07T08:32:21Z
TradMedJ:ART
"151127 2015 eng "
2406-9086
1410-5918
dc
EFFECT OF PUNGENT PRINCIPLE CONTAINING EXTRACT OF Zingiber officinale Roxb. RHIZOME ON MACROPHAGE ACTIVITY OF MALE MICE INFECTED WITH Listeria monocytogenes
Mellawati, Dyah
Faculty of Pharmacy, Universitas Gadjah Mada
Sudarsono, Sudarsono
Faculty of Pharmacy, Universitas Gadjah Mada
Yuswanto, Agustinus
Faculty of Pharmacy, Universitas Gadjah Mada
jahe emprit Rhizome, phagocytosis, macrophage, levamisol, Echinacea Extract
Immunomodulator has been used as one of the attention to develop herbal medicine. Jahe is one of herbal medicine which is usually used to heal various diseases. Jahe Emprit is one of local name of Jahe in Indonesia. The aim of this research was to investigate the effect of pungent principle containing extract of Jahe emprit rhizome on the macrophage activity compared by levamisol and Echinacea extract. Extraction was done by 70%v/v ethanol. The concentrations of extract were 5 mg/kg B.W; 25 mg/kg B.W; and 100 mg/kg B.W. CMC Na 1,5%w/v was used as solvent control. Levamisol 2,5 mg/kg B.W and Echinacea extract 10 mg/kg B.W were used as positive control. The macrophage ability were measured according to Leijh et al., (1986) that was basically on the amount of latex which was captured by macrophage. The extracts were orally administrated to the Swiss male mice at the dose of 0,2 ml/20 g B.W. The Macrophage activity was analyzed by one way ANOVA at 95% of confidence level. The extract which had specification of 35% relative concentration of pungent principles; 3,554% ± 0,145 % W/W total phenolic substances; 13,70 mg/ml antioxydative value (IC50) at the concentrations of 5 mg/kg B.W and 25 mg/kg B.W could increase phagocytosis ability. The ability of the extract at the concentration 25 mg/kg.B.W had no different with either the Levamizol 2,5 mg/kg. B.W., and Echinacea extract 2,5 mg/kg. B.W.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-09-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8135
Majalah Obat Tradisional; Vol 15, No 3 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8136
2015-12-07T08:29:26Z
TradMedJ:ART
"151127 2015 eng "
2406-9086
1410-5918
dc
ACTIVITY OF AMBON BANANA (Musa paradisiaca var. sapientum) STEM EXTRACT IN OINTMENT FORMULATION ON THE WOUND HEALING PROCESS OF MICE SKIN (Mus musculus albinus)
Febram, Bayu
Faculty of Veterinary Medicine, Bogor Agricultural University
Wientarsih, Ietje
Faculty of Veterinary Medicine, Bogor Agricultural University
Pontjo, Bambang
Faculty of Veterinary Medicine, Bogor Agricultural University
ointment, extract, banana stem, wound healing, mice
The objective of the present research is to study the activity of banana stem extract in ointment solutions in the acceleration of wound healing process on mice skins. Totally of 45 mice strain DDY 4-6 weeks old were devided in negativecontrol group (placebo ointment), positive control group (Betadine® ointment) and Ambon banana stem extract ointment.All mice were aseptically wounded 1-1,5 cm in the anterior region of back skin using a sterile scalpel. The wound wassmeared with the ointment. The pathology anatomy observations was done inday 3, 5, 7, 14 and 21 post wounded. Parameters of the gross lesions (pathology anatomy) observations warecolour of the wound, blood coagulations, scab formations and size of the wound. Parameter for (microscopic lesions) histopathology were infiltrations of inflammatory cells (neutrophils, lymphocytes,macrofages), neo-capillarizations, re-epitelization percentage and the thickness of fibroblast. All quantitative data were measure using ANOVA and continue with Duncan Test, moreover, the qualitative data were presented descriptively. The result shows that gross lesions observations, the extract ointment group was faster in scab formations and covers the wound without trace compared to the negative control group. The statistical test on the infiltrations of inflammatory cells parameter of the extract ointment group significantly different (P<0.05) compared to the negative control group. Histopatologycal observations shows that Ambon banana stem extract in ointment solutions can increase the infiltrations of inflammatory cells, neo-capillary formations, re-epitelizations percentage andacceleration of fibroblast formations. Base on the result the Ambon banana stems extract in ointment solutions can accelerate the wound healing process and it seems that this solution could be developed and uses for the medical purposes.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-09-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8136
Majalah Obat Tradisional; Vol 15, No 3 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
oai:jurnal.ugm.ac.id:article/8137
2015-12-07T08:32:21Z
TradMedJ:ART
"151127 2015 eng "
2406-9086
1410-5918
dc
EFFECT GARGLING WITH CENTELLA’S EXTRACT CONCENTRATION 40% AND 50% TO INHIBIT THE SPECIFIC ACTIVITY GLUTATHIONE S-TRANSFERASE IN SALIVA GINGIVITIS PATIENS
Prameswari, Dhika Paramita
Faculty of Dentistry, Universitas Gadjah Mada
Handajani, Juni
Faculty of Dentistry, Universitas Gadjah Mada
gingivitis, saliva, glutathione S-transferase, flavonoid, Centella asiatica (L.) Urban
Glutathione S-transferase (GST) is enzyme in saliva that catalyzes the nucleophilic conjugation reaction of glutathione (GSH) with many diverse electrophilic substrates and has major mechanism in detoxification process of xenobiotics. Pegagan (Centella asiatica (L.) Urban) is contain flavonoid as a xenobiotic component that have an ability to inhibit the specific activity of GST. This study was aiming to understand the effect of gargling with centella’s extract concentration 40% and 50% to inhibit the specific activity of GST in saliva of gingivitis patient. Under ethical clearance from local Ethics Committee from Medical Faculty, Gadjah Mada University, 30 patients of moderate ingivitis divided into two groups: 20 person as treated group were instructed to gargle centella’s extracts concentration 50% and 60%, and 10 person as positive control group was given Hexetidine 0.1%. Every subject was gargling as much as 5 ml every morning (after woke up) and every night (before sleeping) for 5 days constitutively. Saliva was collected before and after treatment. GST’s specific activity was measured using spectrophotometer at 340 nm wavelength and gingivitis status was using Gingival Index (GI). This study showed that using centella’s extract concentration 50% could down regulate the GST’s specific activity saliva and GI score. In conclusion, centella’s extract concentration 50% was indicating better compare with concentration 40% to accelerate of gingivitis healing.
Faculty of Pharmacy, Universitas Gadjah Mada
2010-09-01 00:00:00
https://journal.ugm.ac.id/TradMedJ/article/view/8137
Majalah Obat Tradisional; Vol 15, No 3 (2010)
eng
Copyright (c) 2010 Majalah Obat Tradisional
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